Objective To understand the dietary intake and nutritional status of pregnant women, and try to give a reasonable suggestion to promote the development of fetus. Methods 227 pregnant women in Changsha city were enrolled in this study. Their serum levels of vitamins were detected and their dietary intake were investigated. Results The ratio of the energy which was provided by dietary protein, carbohydrate and fat was appropriate. But the intake of dietary calcium, vitamin B1 and vitamin B2 was very low. With the development of gestation there were a remarkable rise in serum vitamin E level and decrease in serum folacin level, which was extremely obvious in the third trimester. It was about 40% of the pregnant women that the serum vitamin C level was lower than normal level. Conclusions Pregnant women tend to be lack of folacin, vitamin C and vitamin B 2.

Objective: To investigate the effects of resveratrol(RES)on the bone mineral density (BMD) in ovariectomized female rats. Method: Forty-eight SD female rats were randomly divided into 6 groups and treated respectively as follows:group A, sham operated; group B, ovariectomized (OVX); group C, OVX supplemented with 0.03 mg/(kg bw ?d)diethylstilbestrol; and group D, E , F: OVX rats supplemented with RES at 5, 15 and 45 mg(/kg bw ?d). The duration of exposure was 90 d and the BMDs of rats were measured by dual-energy X-ray absorptiometry (QDR-4500A). Results: BMDs at all measured sites of group B were significantly lower than those of group A. And compared with group B, BMDs of the total body, lumbar vertebrae and femur of group D, E, F were increased significantly by RES. Conclusion: The bone loss of the ovariectomized female rats could be inhibited by RES. It seemed that the inhibitory effects of 45 mg/(kg bw ?d)RES on bone loss of ovariectomized female rats were better than the other 2 dosages or 0.03 mg/(kg bw ?d)diethylstilbestrol in this experiment.

Objective To investigate the effect of equol on ERK mediated signal transduction pathway and to clarify its mechanism of proliferation inhibition on human ovarian carcinoma cell line SKOV-3. Method SKOV-3 cells were treated with 10-8,10-7,10-6,10-5,5?10-5,10-4 mol/L equol for 24,48 and 72h. After pretreatment with 10 ?mol/L U0126 an ERK signaling pathway inhibitor or ICI182,780,estrogen receptor inhibitors for 1h,then treatment with 50 ?mol/L equol for 2h,the cell viability was examined and the expressions of ERK and p-ERK protein were determined using Western blotting. Results Equol was demonstrated to inhibit SKOV-3,proliferation time-and dose-dependently. The expression of p-ERK protein was decreased in dose-dependent manner,while the expression of total ERK was unchanged. Both the single use of U0126,or ICI182,780,and combined with equol could decrease the expression of p-ERK protein. Conclusion Equol could inhibit proliferation in ovarian carcinoma cell lines SKOV-3. Its inhibitory effect appears to be due to down-regulation of p-ERK protein.

Objective To evaluate the effects of the different treatments for vitiligo in the special sites. Methods 122 cases (375 leuk oplakiae) of vitiligo in special sites were randomly divided into 5 groups:hair germ grafting (HGG) group (42 cases), resurfacing (RS) group (52 cases), follicular scraping injection (FSI) group (35 cases), liquid nitrogen freezing (LNF) group (30 cases) and external medication (EM) group (45 cases). Efficacy of the treatments was observed and evaluated in different groups and all the data were statistically analyzed. Results Effective rates were 97.1 % in HGG group, 94.7 % in RS group, 59.7 % in FSI group, 57.1 % in LNF group and 45.6 % in EM group.There were very significant differences between different groups ( ? 2 = 111.7, P

Objective To establish a flow cytometry-based assay for the detection of monocyte-me-diated antibody-dependent cell-mediated cytotoxicity ( ADCC ) .Methods P815 cells double stained with PKH26 and carboxyfluorescein succinimidyl ester ( CFSE ) were used as target cells and coated with P 815 specific antibodies to form antigen-antibody complexes .The peripheral blood mononuclear cells were isolated as effector cells and co-cultured with the antigen-antibody complexes .The CD3-CD14+PKH26+CFSE-cell population were gated by flow cytometry .Optimized effector/target cell ratio and incubation time for killing assay were identified .Monocyte-mediated ADCC in 23 patients with chronic HCV infection and 22 healthy subjects were analyzed .Results The monocyte-mediated ADCC could be evaluated through analyzing the CD3-CD14+PKH26+CFSE-cells with flow cytometry .The optimized effector/target cell ratio was 10 ∶1 and the optimized time for incubation was 4 h.Monocyte-mediated ADCC was inhibited in patients with chronic HCV infection as compared with healthy subjects (P=0.009).Conclusion A flow cytometry-based assay for the detection of monocyte-mediated ADCC was established , which could be used as a fast , sensitive and safety method for the evaluation of monocyte-mediated ADCC during viral infections and the research and de-velopment of drugs .

This study was aimed to screen antibacterial agents against 9 pathogenic bacteria from 240 extracts of 60 traditional Chinese medicinals ( TCM ) . And the minimum inhibitory concentration ( MIC ) test was applied on extracts with positive results. The disk diffusion was employed to screen the antibacterial activity preliminar-ily among 240 extracts. The MICs of active extracts were tested by liquid culture method (double dilution method). The results revealed that 104 extracts show antibacterial activity on one or more strains, 20 of them show strong inhibition on three commonly seen bacteria ( MIC < 0 . 2 mg/mL ) . It was concluded that ethanol , acetone and hexane extracts of 11 TCMs including Salvia miltiorrhiza, Cnidium monnieri, Polygonum tinctorium, Taraxacum mongolicum, Morus alba, Glycyrrhiza uralensis, Curcuma longa, Arnebia euchroma, Lobelia chinen-sis , Chrysanthemum indicum and Buddleja officinalis show strong inhibition on Bacillus subtilis , Bacillus cereus , Staphylococcus aureus .

Objective To establish an high performance liquid chromatography method for determination of 2-indole ketone derivative and its related substances. Methods Agilent Eclipse XDB-C18(250 mm×4.6 mm,5 μm) column was adopted.The mobile phase was acetonitrile-water with gradient elution mode at a flow rate of 1. 0 mL?min-1; the column temperature was 35 ℃;the injection volume was 20 μL and the detection wavelength was set at 257 nm. Results 2-indole ketone derivative ID and related substances could be well separated. The 2-indole ketone derivative had good linear correlation ( r=0.999 4) within the range of 40-300 μg?mL-1 . It had a good precision ( RSD<1%) . The limit of detection was 8 ng. Conclusion The method is accurate,simple,sensitive and selective,which can be used for the quality control of 2-indole ketone derivative and related substances.

Objective To explore the value and advantage of intracranial pressure (ICP) monitoring in the treatment of hypertensive intracerebral hemorrhage (HICH) through minimally invasive surgery. Methods Seventy-three HICH cases were randomly selected and then divided into control group and treatment group. Thirty-four of them in control group received soft-channel minimally invasive hematoma removal. The head of the soft-channel was placed in the center of the hematoma. One third to half of the initial hematoma was extracted during the operation. Urokinase was injected into the soft channel to dissolve the hematoma. Thirty-nine of them in treatment group underwent the same operation procedure but with ICP monitoring to control aspirating hematoma during the operation. The target ICP was 15 mmHg. The aspiration of hematoma may stop once the ICP down to the target. Treatment such as urokinase injection was adjusted according to the value of ICP monitoring throughout the operation. The incidence of rebleeding, hematoma evacuation time and the average length of stay between two groups were compared. Results The results showed that one fifth of hematoma extracted was enough for a desired ICP. There found no rebleeding case in treatment group while 4 cases in control group and the difference had statistical significance. The hematoma evacuation time and the average length of stay between two groups had statistical significance. Conclusion Continuous ICP monitoring combined with directional software channel minimally invasive surgery changes the concept of empirically intraoperative hematoma evacuation and postoperative drug injection and proposes the idea of controlling drainage. It can shorten the course, provide sensitive and objective indicators and basis, reduce the secondary brain injury and improve prognosis.

Objective To investigate the effect on free portal pressure (FPP), postoperative complications and rebleeding of devascularization procedure with intact portasystemic collateral shunts. Methods The clinical data of 121 patients with portal hypertension undergoing devascularization in our hospital from 1994 to 2004 were retrospectively analyzed. Patients were divided into two groups(96 cases and 25 cases) , respectively according to whether or not the portasystemic collateral shunts were reserved during operation. Results (1) Free portal pressure (FPP) decreased significantly after devascularization in both two groups [(47?8) cmH2O vs. (37?5) cmH2O, (41?7) cmH2O vs. (37?6) cmH2O; P

OBJECTIVE To investigate the distribution of acute appendicitis pathogens and their drug resistance,and to guide clinically the selection of reasonable antibiotics.METHODS Germicultures and drug sensitivity tests were conducted for the pus specimens from the 116 patients with acute appendicitis by ATB Expression bacteria(identifier).RESULTS Pathogens in 75 cases of 116 were found,with a positive rate of 64.7%,totally 85 strains of bacteria were isolated from clinical samples.Gram-negative bacilli had 72 strains,accounted for 84.7% and Gram-positives had 13 strains,(accounted) for 15.3%.The first three were Escherichia coli(54.1%),Pseudomonas aeruginosa(4.7%),and Klebsiella pneumoniae(4.7%).Sensitivity tests showed that resistance rates of E.coli to cefapirin,imipenem,compound(piperacillin),cefoxitin,and amikacin were 0,2.2%,10.9%,17.4%, and(23.9%).CONCLUSIONS Dominant pathogens in patients with acute(appendicitis) are intestinal bacilli,with(predominance) of E.coli;more emphasis should be placed on pathogen detections and drug sensitivity tests so that effective antibiotics could be selected and resistant bacteria be reduced and nosocomial infections be prevented.