Objective To evaluate the clinical outcomes of open reduction and locking plate fixation with or without auto-iliac graft for humeral shaft nonunion.Methods Between January 2008 and May 2012,65 cases of humeral shaft nonunion were treated in our department.They were 48 men and 17 women,24 to 54 years of age (39.8 years).Of them,43 were atrophic and 22 hypertrophic.The radial nerve was exposed and protected during operation.After the nonunion portion was debrided,the humeral length was recovered.Next the fracture sites were fixated by a locking plate.The defected area was filled with auto-iliac graft if necessary.The visual analogue scale (VAS) scores and The Short Form-36 (SF-36) scores were documented after operation; the functional recovery was evaluated using Constant scoring at the last follow-up.Results The patients obtained a mean follow-up of 13.8 months (from 10 to 32 months).Of them,50 achieved clinical bone union after an average of 3.8 months (from 3 to 7 months).The remaining 6 cases showed no sign of union 9 months postoperatively.Of the 50 patients with union,the mean VAS score was decreased significantly from the preoperative 5.1 ± 3.2 points to the postoperative 1.4 ± 0.8 points,and the mean SF-36 score was increased significantly from the preoperative 45.7 ± 16.9 points to the postoperative 72.9 ± 18.5 points (P ＜ 0.05).The function was scored at the last follow-up as excellent in 34 cases,good in 12,fair in 2 and poor in 2,giving an excellent to good rate of 92.0％.The radical nerve injury was repaired within 3 months postoperation in 3 patients.Rotation deformity occurred in 3 cases without obvious functional limitation.No implant complications such as plate breakage and screw loosening were observed.Conclusion Open reduction and locking plate fixation with or without auto-iliac graft is one effective treatment of humeral shaft nonunion,but damage to the radial nerve should be avoided and fixation of 6 layers of cortex should be maintained at both fracture ends during operation

Objective: To study the anatomic structure of the nasopalatine duct and the neighboring bone,provide reference for implant surgery in the incisor region. Methods: Manual quantitative measurement of the nasopalatine duct were performed in 73 maxilla specimens (38 from male and 35 from female). Results: The angle between the anterior wall of nasopalatine duct and the nasal floor was 114.80??11.00?, the angle between the cortical plate of anterior maxilla and the nasal floor 107.28??10.75?, the length of the nasopalatine duct was (15.63?2.18) mm in male, and ( 14.59 ?1.96) mm in female, the width of nasal opening of the duct was ( 4.92 ?1.46) mm in male, and (5.73?1.50) mm in female, the width of oral opening of the duct was (3.23?0.60) mm in male and (3.83?0.92) mm in female, at the level of nasal floor, the distance between the anterior wall of the duct and cortical plate of anterior maxilla was (6.15?1.90) mm. Conclusion: In front of nasopalatine duct, implants should be implanted parallel to nasopalatine duct,with an angle about 115? between the implant direction and nasal floor. The bone in front of nasopalatine duct was thin,the diameter of implants applied here should be less than 4 mm.

Objective To investigate the response of multiple myeloma (MM) cells to 8-chloroadenosine 3',5'-monophosphate (8-Cl-cAMP) and the impact of arsenic trioxide (As2O3) on the above reaction.Methods MM-derived cell lines RPMI8226 and U266 were used as in vitro models.Cell apoptosis was evaluated according to cellular morphology and DNA content measured by flow cytometry.Meanwhile,rhodamine 123 (Rh123) staining and flow cytometry assay were used to detect the changes of mitochondrial transmembrane potentials (△ψm) in MM cells before and after the treatment.The synergic effects of 8-Cl-cAMP and As2O3 were evaluated by King' s formula.Results The 8-Cl-cAMP could induce growth inhibition of RPMI8226 and U266 cells in dose and time-related manners.The 8-Cl-cAMP could trigger apoptosis and △ψm collapse in MM cells through cellular morphology and flow cytometry analysis.As2O3 accelerated 8-Cl-cAMP-mediated apoptosis of RPMI8226 cells,but there were few synergic effects observed.Conclusion 8-Cl-cAMP could induce cell proliferation inhibition and apoptosis in MM cells.Mitochondria may be one of targets in 8-Cl-cAMP-mediated apoptosis.Furthermore,As2O3 catalyzes 8-Cl-cAMP-induced apoptosis.

BACKGROUND: The rehabilitation of maxillary loss is based on maxillary prosthesis,and the most difficult problem is that retention and support of maxillary prosthesis can hardly be achieved. The employment of implants in the zygoma region provides good basis for the retention and support of maxillary prosthesis. However,during implantation in the zygoma the pe ripheral tissues may be injured, and even induce failed implantation.OBJECTIVE: To study the bone structure of the zygoma so as to provide reference for implantation in this region.DESIGN:Randomized sampling for repeated observation measurement based on maxilla specimens.SETTING: The research was completed in the prosthodontic department of stomatological college and department of human anatomy of a military medical university.PARTICIPANTS:The experiment was carried out in the Department of Human Anatomy,Fourth Military Medical University of Chinese PLA from March to May 2002. A total of 73 adult maxilla specimens(38 from males and 35 from females) were provided by the Department of Human Anatomy,Fourth Military Medical University of Chinese PLA.METHODS: Quantitative measurement of bone structure of the zygoma region was made with the cursor ruler in the 73 specimens. T-test was used to analyze the measurement data.MAIN OUTCOME MEASURES: The data of measurement: bone thickness of the zygoma, width of the lower part of the zygoma, length of the zygoma, and the distance between lower part of the zygoma and orbital floor.RESULTS: ① Bone thickness gradually decreased from the interior-inferior borderline to the exterior-superior part of the zygoma. In males, the average bone thickness 10 mm,12 mm,14 mm and 16 mm away from the interior-inferior borderline of the zygoma was 6.97 mm, 6.45 mm, 5.75 mm and 5. 01 mm, respectively, in the upper part, and 7.03 mm, 6.69 mm, 6.28 mm and 5.89 mm in the lower part. In females, the average bone thickness 4 mm,6 mm, 8 mm and 10 mm away from the interior-inferior borderline was 6.95 mm,6. 55 mm,6.28 mm and 5.31 mm in the upper part,and 6.60 mm,6. 39 mm,6.26 mm and 5.75 mm in the lower part. ② The lower part of the zygoma was 21.39 mm wide in males and 17.20 mm in females. ③ The average length of the zygoma was 21. 12 mm in males and 19.58 mm in females. ④ The average distance between lower part of the zygoma and orbital floor was 4. 17 mm in males and 3.71 mm in females.CONCLUSION:In maxillary loss,the zygoma is an optimal region for double implants at a distance of more than 15 mm. In males, the implants of 3.6 -4.0 mm in diameter and 10 - 12 mm or 14 - 16 mm in lengthcan be employed; in females, the implants of 3.4 - 3.8 mm in diameter and 4 - 6 mm or 8- 10 mm in length can be employed.The differences in bone quantity between males and females are statistically significant in the zygoma,males' better than females', so they should be treated differently during clinical practice.

Objective To evaluate the clinical efficacy and toxicity of EPOCH regimen in the treatment of elderly patients with peripheral T-cell lymphoma. Methods Twenty-eight elderly patients with pathologically diagnosed peripheral T-cell lymphoma were treated with EPOCH regimen, including 96-hour continuous infusion of etoposide 50 mg/m2, epirubincin 12 mg/m2 and vincristin 0.4 mg/m2 on daysl through 4,cyclophosphamide 750 mg/m2 given as intravenous bolus on day 5 and prednisone 60 mg/m2 administered orally on daysl through 5. The EPOCH regimen was repeated very 21 days. Clinical efficacy and safety profiles of EPOCH regimen was systemically reviewed and analysed. Results All the 28 patients received a total of 85 cycles of EPOCH regimen. The median cycles was two courses. Fifteen patients achieved complete response, while five cases obtaining partial response. The overall response rate was 71.4 %. The median survival time was 20 months. In newly diagnosed patients, complete response rate (CR) as well as partial response (PR) and overall response rate (OR) reached 64.7 %, 23.5 % and 88.2 %, respectively, which was significantly higher than that in refractory cases, whose CR, PR and OR were 36.4 %, 9.1% and 45.5 %(λ 2 = 5.99, P ＜0.05). In addition, the median survivalduration of newly diagnosed patients was longer than that of refractory cases, whose median survival time was 24 and 13 months, respectively. The major adverse events was myelosuppression with grade 3-4 neutropenia and thrombocytopenia in 53.6 % and 50.0 % cases.Non-hematologic toxicities were moderate and uncommon. The frequency of adverse effects in de novo patients showed little difference in comparison with that in refractory ones (P＞0.05). Conclusion EPOCH regimen was an effective and well tolerated therapeutic schedule for elderly patients with peripheral T-cell lymphoma.

Objective: To evaluate the physical properties of SY-1 silicone elastomer with various ratios of cross-linker to base. Methods: The ratios of cross-linker to base were set at 0.5 %、1 %、 1. 5 %、 2 % 、2. 5 % respectively while other conditions were kept same, then the major physical properties of the elatomer were studied with a tensile tester and ASTMD 2240 method. Results: The elongation(%) due to tension, tensile strength (MPa), laceration strength (kN/m) and hardness (A) of SY-1 silicone elastomer with the ration of 0. 5% of cross-linker to base were 540?47,4. 3?0. 28, 14. 4?2.4 and 18. 0?3. w; those of that with 1% 440 ?54,4.0?0.32,18. 2?3.1 and 24. 0?3. 61 with 1.5% 430?51, 4.1?0. 29, 13.6?2.2 and 25.0?2.1; with 2% 350?46, 2.5?0. 33, 9.0?2.1 and 28.0?1.9; with 2.5% 280?36, 2.0?0. 3, 11.0?2.5 and 29.0? 2.8, respectively. Conclusion: The physical properties of SY-1 silicone elastomer with a ratio of 1% of crosslinker to base are more suitable for prothesis fabrication.

Objective:To study the stress distribution of Magsoft (cushion type) and Magnedisk magnetic attachment. Methods: 2 overdentures were prepared on each of 5 edentulous jaw models. The overdentures were pared and retained with Magnedisk and Magsoft magnetic attachments respectively.Photoelastic analysis was used to study the stress distribution for the implanted complete overdentures. Results: Under the same load, around the implants, the stress under the overdentures retained with Magsoft was lower than that with Magnedisk. Compared with Magnedisk, the stress around the abutments under the overdenture retained with Magsoft were distributed in a more even manner. Conclusion: Magsoft magnetic attachment has better biomechanical characteristic than Magnedisk and can distribute stress more evenly throughout the whole supporting structure for its special cushioned design.

Objective:To investigate the clinical characteristics and outcome of novel coronavirus pneumonia (COVID-19)patients with different body mass index (BMI), and to provide the basis for disease assessment and prognosis.Methods:The clinical data of 541 patients with COVID-19 diagnosed in Xiaogan Hospital Affiliated to Wuhan University of Science and Technology from January 16 to March 28, 2020 were collected. The patients were divided into normal weight group, overweight group, and obesity group according to BMI. The clinical characteristics and outcomes of the three groups were compared. The correlation between BMI and clinical classification was analyzed by ordinal logistic regression.Results:There were 288 cases (53.23%) in normal weight group, 193 cases (35.67%) in overweight group, and 60 cases (11.09%) in obesity group. Compared with normal weight group, overweight and obesity groups displayed higher proportion of hypertension, with increased levels of white blood cells, neutrophils, C reactive protein, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and triglyceride in laboratory test results, and higher proportion of severe and critical illness ( P<0.05 or P<0.01). After adjusting for age, gender, and underlying diseases, regression analysis showed that higher BMI predicted more severe clinical classification ( OR=1.079, 95% CI 1.010-1.154). Conclusion:In COVID-19 patients, overweight and obese patients are more likely to develop into severe and critical illness, suggesting that obesity may be an important risk factor affecting the clinical outcome of COVID-19.

OBJECTIVE:To virtually screen lignanoid compounds with inhibitory effect of histone deacetylase(HDAC)by vir-tual screening method. METHODS:Using“lignanoid”as keyword,requiring CNKI,VIP,PubMed and other database,lignanoid compounds were collected as ligand to establish ligand base, histone deacetylase receptor HDAC2 (PDB code:4LXZ) and HDAC8 (PDB code:1T69) were selected from PDB database,and then ligands and 3D active site of receptors were docked by SYBYL-X 2.0 software. The affinity of receptors to ligand was reflected by total score. RESULTS:345 lignanoid compounds, 4LXZ and 1T69 primary ligand were used to establish ligand base which included 347 ligands. Ligands No.275,271,110,200, 056,258,181,129,037,270,187 were demonstrated good affinity with receptors HDAC2 and HDAC8. Ligands and receptors residue were docked via hydrogen bond. CONCLUSIONS:Lignanoid compounds have inhibitory effect on HDAC;virtual screen-ing method is effective in natural product activity prediction,which can provide quick access to and theoretical guidance for new pharmacological studies of lignanoid compounds.

Objective To reveal the pathophysiological changes of sepsis, the plasma metabonomics of septic rats was determined and differential metabolites were determined by gas chromatography-mass spectrometry (GC-MS). Methods Male Spraguee-Dawley (SD) rats about 8 weeks were randomly divided into sham group (n = 18) and sepsis group (n = 24). Cecal ligation and puncture (CLP) was used to build sepsis model, while cecum was kept intact only in the sham group. 6 hours after the operation, rats were anesthetized, and blood was harvested through heart thoracotomy. Then the plasma metabonomics was detected by GC-MS and metabolic profile analysis was performed to find the relative differential metabolites.Results Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) showed that the metabolic profiling of the sepsis group was significantly different from the sham group. 259 kinds of metabolites were got by GC-MS, and 69 kinds of differential metabolites were found between sham group and sepsis group, in which 23 differential metabolites were related to amino acid metabolism. Compared with sham group, the contents of putrescine, N-glutamic acid, hydroxynorvaline, 3-cyanuric acid, D-alanyl-D-alanine and urea in the plasma of septic rats increased significantly, which ratios of sepsis/sham group were 10.876, 6.394, 2.800, 2.226, 1.323, 1.203, respectively (allP < 0.05). On the other hand, the contents of oxygen generation of proline, citrulline, glutamine, su-beta-hydroxy aspartic acid, citric acid, N-methyl-DL-alanine, serine, lysine, threonine, N-formyl-L-methionine, methionine, alanine, nicotinuric acid, N-methyl-L-glutamic acid, trans-4-hydroxy-L-proline, proline, L-glutamic acid in the plasma of septic rats decreased significantly, which ratios of sepsis/sham group were 0.858, 0.853, 0.834, 0.816, 0.816, 0.814, 0.813, 0.801, 0.793, 0.792, 0.774, 0.766, 0.748, 0.727, 0.716, 0.674, 0.603, respectively (allP < 0.05).Conclusions Through the GC-MS analysis of plasma metabonomics of septic rats, we found abnormal energy metabolism changes. The content of amino acid in plasma might be a method to evaluate the energy metabolism status of sepsis.