Objective: To explore the operation method and clinical effect of treatment for bilateral cleft lip with improved straight line closure. Methods: On the basis of straight line closure, the vermilion and anterior lip pear were repaired with the muscle-vermilion labial flap of lateral lip. In order to close nasal floor, flap-C from lateral wall of anterior lip and flap-G from nasal wing base were designed, meanwhile, nasal mucosa flap-D and flap-H from two sides of nasal base fissure were designed. The function of the orbicularis oris was repaired by cutting off the anomaly adhesion of the orbicularis oris. Results: The post-operational upper lip was thickened together with good shape and vermilion color, there was no obvious dynamic abnormality, nasal floor was closed well. Conclusion: The improved straight line closure can obtain good shape of lip, reduce the whistling deformities, oronasal fistula and dynamic abnormality.

Objective To assess the prediction value of Pmax and Pd on idiopathic paroxysmal atrial fibrillation(IPAF) and the possible mechanism of amiodrone preventing the recurrence of IPAF.Methods Pmax and Pd were measured in 60 patients with IPAF and 60 healthy control subjects by 12-lead surface electrocardiography. 30 of patients with IPAF were assigned to treatment with amiodrone for 6 months. Pd?Pmax and recurrence of IPAF were observed and compared with control group during the treatment.Results Pmax and Pd were found to be significantly higher in patients with IPAF than in healthy subjects[(126?17)ms vs(102?11)ms; (50?9)vs(29?8)ms,P

[Objective]To study the biocompatibility of nacre on cultured human osteoblasts in vitro.[Method]The iliac bone of human were added with collagenase-trypsin,a lot of osteoblasts were released out.Then the osteoblasts were purified and cultured.Subsequently,the 3rd generation cultured human osteoblasts were inoculated with the extract of three materials:nacre,hydroxyapatite,and rubber.The appearance of osteoblast was observed under the phase contrast microscope.Microstructure of the osteoblast was observed under transmission electron microscope.Additionally,to determine whether these three materials affect cell proliferation,the numbers of BrdU-labeled cells were examined at three groups.MTT assay was tested to observe the viability of human osteoblasts.[Result]Under the phase contrast microscope,the appearance of the osteoblasts in two groups were spindle or triangle like,and black nodes were seen in confluent cell layer 19 days after cultured.The observation of electron microscopy showed that the nucleus of the osteoblasts in two groups were big and round,and there were a large number of mitochondrion and rough endoplasmic reticulum in two groups.Stereological analyses of the numbers of BrdU-labeled cells revealed that females produced more cells than males in the dentate gyrus but not in the subventricular zone.[Conclusion]The nacre have no harmful effect to character of biology of human osteoblasts in vitro.

Objective To analyze the problem existing in the process of health records informatization con-struction and explore solution on the basis of situation of health record informatization in Yanta district of Xi'an city. And to provide bases for acceleration of residents'health records informatization construction in Xi'an.Methods Send 10 investigators to study and investigate the hospital information management system of 10 full-time public universities in Yanta district.Results There were a total of 25 711 faculties in 10 full-time public universities and 19 809 facul-ties had established electronic files,the filing rate was 77.04% A.On the whole,it seemed that the teaching staff in the filing rate(78.91%)was higher than that of the retired workers filing rate(73.78%),the two groups had statisti-cally significant difference(χ2 =88.164,P <0.05)B.Conclusion Xi'an Yanta district university faculty of electron-ic health records has exceeded the national average level,but there are still many problems need to be improved.We should strengthen government investment,the establishment of the region's unified information management standards, increase publicity and education efforts,and actively create the region's resource information sharing platform.

BACKGROUND: It is of great significance to understand the effects of different components of blood pressure on the occurrence of cerebrovascular diseases and administer proper decompression treatments in various situations.OBJECTIVE: To study the relationship of increased pulse pressure with the occurrence and severity degree of cerebral infarction by analyzing the data of pulse pressure recorded in hospitalized patients with acute cerebral infarction.DESIGN: Case-control analysis.SETTING: First Affiliated Hospital of Harbin Medical University.PARTICIPANTS: A total of 300 patients hospitalized at the First Affiliated Hospital of Harbin Medical University between June 2002 and January 2003 for ischemic cerebral infarction were enrolled, including 196males and 104 females aged (57.9±11.9) years. Another 199 persons who came to the same hospital for physical examination at the same period were set as normal group, including 110 males and 89 females aged (55.9±12.4)years.METHODS: ① Measurement of blood pressure: Systolic pressure and diastolic pressure of each person were recorded with mercury sphygmomanometer at the site of brachial artery of the right upper extremity and pulse pressure was calculated. ② Evaluation of neurological impairment:Upon hospital admission, each patient with ischemic cerebral infarction was evaluated with the stroke scale made by the National Institute of Health (NIHSS), in which a higher score means more severe infarction.MAIN OUTCOME MEASURES: ① Comparison of blood pressure parameters of subjects in the two groups. ② Distribution of different pulse pressure levels in the two groups. ③ Comparison of pulse pressure of subjects of different age in the two groups. ④ Results of non-parameters tests of neural function loss in cerebral infarction patients with different pulse pressure levels.RESULTS: ① Mean systolic pressure: It was significantly higher in the case group than in control group [(152±22), (133±19) mm Hg, t' =10.494,P ＜ 0.01]. Mean diastolic pressure: It was higher in the case group than in control group [(93±14), (81±11) mm Hg, t' = 10.129, P ＜ 0.01]. Meanpulse pressure: It was higher in the case group than in control group [(59.61±11.86), (51.93±14.10) mm Hg, t' =5.612, P ＜ 0.05]. Pairwise corre lation analysis showed that Pearson correlation coefficient between pulse pressure and systolic pressure was 0.789 (P ＜ 0.01); Pearson correlation.coefficient between pulse pressure and diastolic pressure was 0.169 (P ＜ 0.01). Therefore, there was a close correlation between pulse pressure and systolic pressure. ② Pulse pressure was distributed between 60 mm Hg and 69 mm Hg in most subjects in the case group, which accounted for 27.7%.It was 40-49 mm Hg in control group, which accounted for 35.7%. It indicated that the incidence rate was high when the pulse pressure was over 60 mm Hg in cerebral infarction. ③ Pulse pressure increased with age.The level of pulse pressure in 40-69 years case group was higher than that in 40-69 control group [(54±16), (45±9)mm Hg, t=4.86, P=0.000]. ④ Patients with cerebral infarction of different levels of pulse pressure were given non-parameter tests of neurological impairment evaluation. The resuits of Kruskal-Wallis test were χ2=4.779, P=0.572 ＞ 0.05; results of median test were as x2=8.365, P=0.213 ＞ 0.05. The results of the two non-parameter tests suggested that there was no significant differences in hospitalization evaluation, that is, although the pulse pressure increased obviously in cerebral infarction, the degree of increase had no correlation with the severity of neurological impairment.CONCLUSION: The increase of pulse pressure is related to the occurrence of cerebral infarction and is also an important factor for evaluating cerebral infarction. However, pulse pressure change is not related to the severity of cerebral infarction.

Objective: To investigate the effects of enamel matrix pr ot eins(EMPs) and purified EMPs(EMD) on the proliferation and protein synthesis act ivity of rat ectomesenchymal stem cells in vitro. Methods: C ell culture technique and 3H-leucine label assay were used to measure the pr oliferation and protein synthesis activity of rat ectomesenchymal stem cells exp osed to the EMPs- or EMD-conditioned culture media with different concentratio n. Results: Both EMPs and EMD at 50～200 mg/ml increased the pro l iferation and protein synthesis of the cells in 10-day-culture. EMPs and EMD a t 150 mg/ml showed the strongest effects(P

Objective Studies on IMRT technique demonstrates that the improved dose homogeneities throughout the breast as well as reduction in dose to the heart and the ipsi-and control-lateral lungs. Methods Based on the physical principle of rolling tangential technique, a treatment plan for a patient has been planned by using Pinnacle aperture-based inversion software. The rolling tangential IM-beam was pre-determined at each pre-selected gantry angle with normally angle separation of 5?, 10? or 15?. Each beam's width was so chosen that the inner-and outer-edge of the beam should be tangential to the inner chest wall and target outer surface respectively. For the starting and ending tangential beam, a pair of opposing beam was designed. The beam's orientation was suggested to be reversed to its opposite angle when the tangential beam's gantry angle reached 90? or 270?. Each beam's shape and size were adjusted on its BEV map. For each tangential beam, 7 sub-segment beams were chosen for each tangential beam. Then, planning system was allowed to do optimization according to the clinical dose prescriptions. Results The calculated dose distributions in target(CTV), left & right lungs, and heart showed that the volume received the prescribed dose 50?Gy V_ 50 was 0.95; The volume in target enclosed with 95%, 105%, 110%, 115% isodose lines V_ 95% , V_ 105% , V_ 110% , V_ 115% was 0.98, 0.84, 0.53, 0.18, respectively. The volume of V_ 10 ,V_ 20 , V_ 30 , V_ 40 V_ 50 , in the lungs and heart were significantly decreased when compared to the current IMRT techniques published in the literature(Table 1).Conclusions The rolling tangential IM-segment irradiation technique for the breast developed by the author is able to offer not only a better dose distribution and better dose conformity throughout the target, but also reduce significantly the dose to the lungs and heart, with the patient's set up and localization process being very simple. The treatment planning for individual patient is optimal in any treatment planning system with Aperture-Optimization inverse software. This technique is certainly to offer more benefit to the patient if some measures are taken to limit or to follow the movement of the chest wall during irradiation.

Objective To investigate the application value of pancreatic duct guide wire and transpancreatic septotomy with precutting technique in difficult endoscopic retrograde cholangiopancreatography.Method258 patients who underwent difficult endoscopic retrograde cholangiopancreatography from April 2014 to April 2016 were selected as study subject, 128 patients among them received the technique of pancreatic duct guide wire, the other 130 patients received transpancreatic septotomy with precutting techniques. The success rate, intubation time and incidence of complications were compared between these two methods for cannulation.Result There was no signiifcant difference in preoperative clinical data between the two groups, the success rate did not differ signiifcantly between the two groups (93.75 % vs 93.85 %). Compared with transpancreatic septotomy with precutting techniques group, pancreatic duct guide wire group is less intubation time consuming (5.92 ± 0.69 vs 12.81 ± 3.67) min, the difference was statistically significant (t = -2.27,P < 0.05). 25 patients experienced complications, with 6 cases of acute pancreatitis, 2 cases of biliray tract infection in pancreatic duct guide wire group, and 8 cases of acute pancreatitis, 3 cases of hemorrhage,6 cases of biliray tract infection in transpancreatic septotomy with precutting techniques group. The pancreatic duct guide wire group had a signiifcantly lower incidence of complications (6.25 % vs 13.08 %). the difference was statistically signiifcant (χ2 = 3.27,P < 0.05). The incidence of acute pancreatitis did not differ signiifcantly between the two groups (4.69 % vs 6.15 %).ConclusionsPancreatic duct guide wire and transpancreatic septotomy with precutting techniques both can further improve the success rate of bile duct cannulation with ERCP. The incidence of acute pancreatitis did not differ significantly between two groups. But pancreatic duct guide wire group is less intubation time consuming, and had a significantly lower incidence of complications. Because of the convenience and safety of the pancreatic duct guide wire technique, and the insertion of the pancreatic duct does not increase the risk of postoperative acute pancreatitis. We think that this method is more worthy of Clinical promotion.

Objective To detect the expression of peripheral blood free prostate specific antigen (fSPA),total prostate specific antigen (tSPA),prostate specific antigen density (PSAD) and prostate cancer gene 3 (PCA3) in prostate disease,and the significance of combined detection of fSPA,tSPA and PCA3.Methods 67 patients with prostate cancer,75 patients with prostatic hyperplasia and 70 healthy male were selected as the research objects from Dec.2014 to Jul.2016.The serum level of fSPA and tSPA was detected by chemiluminescence immune staining method.The prostate volume was tested by ultrasonic sound and PSAD value was calculated.The total RNA was extracted by Trizol,and the serum PCA3 mRNA expression was detected by RT-PCR.The specificity and sensitivity of combined detection of fSPA,tSPA and PCA3 were analyzed.Results The serum levels of fSPA,tSPA,PSAD and PCA3 in prostate cancer were significantly higher than those in patients with prostatic hyperplasia and healthy male,and they were higher in patients with hyperplasia of prostate than in healthy male,and the differences had statistical significance (P＜0.01).The serum levels of fSPA,tSPA,PSAD and PCA3 were higher in patients with Gleason score ≥7 points and in T3-T4 stage than in patients with Gleason score ＜7 and in T1-T2 stage,and the difference had statistical significance (P＜0.01).The serum levels of fSPA,tSPA,PSAD and PCA3 were positively correlated with Gleason score and TMN pathological stage,and the difference had statistical significance (P＜0.01).The AUC value of fSPA,tSPA,PSAD and PCA3 in diagnosis of prostate cancer was 0.53,0.57,0.63 and 0.75,and the AUC value of combined detection was 0.92.The combined detection efficiency was higher than the single index.The specificity of fSPA,tSPA,PSAD and PCA3 was 67.16％,68.66％,73.13％ and 85.07％,and the sensitivity was 71.64％,70.15％,74.63％ and 82.09％ respectively.The specificity of combined detection was 97.01％,the sensitivity was 92.54％,and the difference had statistical significance (P＜0.01).Conclusion The serum level of fSPA,tSPA,PSAD and PCA3 is increased in prostate disease,and is negatively correlated with Gleason score and TMN pathological stage.The combined detection of fSPA,tSPA,PSAD and PCA3 can improve the sensitivity and specificity of prostate disease diagnosis,and is of high clinical value.

Objective To investigate the correlation between Dishevelled protein expression and the proliferation and invasion of glioma cells.Methods 67 cases of brain glioma specimens were collected.The expression of Dishevelled protein was detected with immunohistochemical method.The immunoreactivity score (IRS) of Dishevelled protein,and proliferation index (PⅠ) and invasion index (Ⅱ) were measured and their correlations were analyzed.Results The positive rate of Dishevelled protein in glioma was 65.7 ％ (44/67).IRS,PⅠ and Ⅱ were 4.15±3.13,(30.93±17.92) ％,(20.38±13.36) ％,respectively.Both PⅠ and Ⅱ significantly increased with an increase in the pathological grade of brain glioma (P ＜ 0.001).Furthermore,PⅠ and Ⅱ were significantly higher in the Dishevelled protein-positive group than those in the Dishevelled protein-negative group [(38.27±17.60) ％ vs (16.02±8.92) ％ of PⅠ and (30.03±13.81) ％ vs (10.63±4.41) ％ of Ⅱ,respectively,P ＜ 0.001].PⅠ and Ⅱ of glioma cells were positively correlated with IRS of Dishevelled protein (r =0.940 between PⅠ and IRS,and r =0.953 between Ⅱ and IRS,respectively).Conclusion Dishevelled protein plays an important role in the proliferation and invasion of brain malignant glioma.