let-7e is one member of let-7 miRNA family, a class of endogenous noncoding RNA which consists of 22 nucleotides. The recent miRNA profiles investigation and analysis results showed that let-7e could be involved in embryonic stem cells differentiation, tumorigenesis in cancer, temporal lobe epilepsy and sleep loss, lipopolysaccharide induced inflammation by regulating its target mRNA expression. This paper gives a review of the important biological function of let-7e.

Objective To explore the effects of repetitive transcranial magnetic stimulation (rTMS) on hippocampus metabolic changes in rats with temporal lobe epilepsy. Methods A chronic temporal lobe epilepsy model was established by use of lithium-pilocarpine in rats. The hippocampus metabolism of the rats were detected by 1H-MRS method before and after the rTMS and compared with that of the normal controls. Relative ratios of N-acetylaspartate (NAA), choline (Cho), glutamate (Glu) and ?-aminobutyric acid (GABA) over creatine (Cr) were calculated. Results Compared with normal control rats, NAA/Cr and GABA/Cr ratios decreased significantly and Cho/Cr ratios increased significantly in hippocampus of the rats with temporal lobe epilepsy. After rTMS NAA/Cr and GABA/Cr ratios increased significantly and Cho/Cr ratios decreased significantly in hippocampus of rats with temporal lobe epilepsy. However, rTMS did not significantly affect the Glu/Cr ratio in hippocampus of the rats with temporal lobe epilepsy. Conclusion rTMS could improve the hippocampus metabolism and exert a neuroprotective effect against brain damage induced by epilepsy.

Long-term potentiation(LTP)is an important form of synaptic plasticity and an objective indicator to investigate learing and memory synaptic mechanisms.With the development of brain slice technology,more and more experiments associated with LTP are carried out on brain slices,which aim to investigate the mechanism in biology and the change in physiology or biochemistry are carried out on the brain slice.This paper gives an overview of recent advances in research of LTP with technology of brain sliceby suchexamples as follows:The regulated expression mechanisms of long-term potentiation at CA1 synapses,the characteristics of LTP induced in hippcampal slices and its relation with the slice-recovery conditions,the enhancement of the magnitude of early longterm potentiation at CA1 hippocampal synapse by the activation of dopamine receptor,and the enhancement of associative long-term potentiation by the activation of β-adrenergic receptors at CA1 synapses in rat hippocampol slices.

BACKGROUND:Meniscus injury is a common disease, whose curative effect is not obvious. The clinical application of tissue engineering technology is gradual y rising, and mesenchymal stem cells-stents complex is expected to be used for the treatment of meniscus injury.
OBJECTIVE:To review the application process of mesenchymal stem cells-stents complex in meniscus injury, in order to provide reference for further study and clinical practice.
METHODS:A computer-based online search of PubMed database, VIP database, Wanfang database and China National Knowledge Infrastructure between 1980 and 2013 was performed to search related articles on mesenchymal stem cells-stents complex in meniscus injury. The key words were“mesenchymal stem cell, meniscus injury, tissue engineering, scaffold, stent”in English and“mesenchymal stem cell, meniscus injury, tissue engineering”in Chinese. For the articles in the same field, we preferred the ones published recently or in authorize journals. Meta-analysis and repetitive studies were excluded. 282 articles were included, and final y 59 articles were included according to inclusion criteria.
RESULTS AND CONCLUSION:Nowadays, numerous experiments showed that mesenchymal stem cells could differentiate into chondrocytes both in vivo or vitro. Mesenchymal stem cells-stents complex could be effective to meniscus injury. Although this technique is stil in exploratory stage, it has promising application prospects in treatment of meniscus injury, because it is characterized by convenience to col ect samples, vitality and no antigenicity.

Objective To explore the expression of plasminogen activator inhibitor-3 (PAI-3) in normal adult skin. Methods Immunohistochemistry and RT-PCR were used to detect the expressions of PAI-3 and uPA. Results mRNA of PAI-3 and uPA were detected. PAI-3 was mainly expressed in stratum basale, stratum spinosum and stratum granulosum, and its expression enhanced in the well differentiated keratinocytes of the epidermis. uPA was located in basal layer. Conclusion PAI-3 exists in normal adult skin and is related with the differentiation of epidermal keratinocytes.

Objective To study the regulating roles of PAI 2 in the differentiation mechanism of the human epidermis. Methods Human skins were take from the early, middle and late human embryos respectively and observed with immunocytochemistry and in situ hybridization techniques. The cell culture and dot blot were also used in the observation of the materials from late embryo. Results 1 PAI 2 exhibits a very high experssion in the development of embryonic period, with the highest level in the middle embryonic phase while the transcripts of PAI 2 still keep a rather high level in the late human embryonic stage. 2 PAI 2 is mainly localized in the superficial, more differentiatied layers of the epidermis.3 PAI 2 is localizated in peripheral cytoplasm of the vitro or vivo keratinocyte.Conclusion PAI 2 is involved in the regulation of the keratinocyte differentiation. [

AIM: To investigate the effect of electro-acupuncture (EA) at Zusanli (ST36) on proinflammatory factors induced-multiple organ dysfunction in rats with sepsis. METHODS: Sixty four male Wastar rats were used to develop the sepsis model by cecal ligation and puncture (CLP). The animals were randomly divided into 4 groups (n=16 in each group): CLP+EA (CLP/EA), CLP+sham EA (CLP/SEA), vagotomy+ CLP+SEA (VA/CLP/SEA) and vagotomy+CLP+EA (VA/CLP/EA). Zusanli point (ST36) was electroacupunctured with constant voltage (2-100 Hz, 2 mA for 0.5 h) 20 min after CLP surgery. Bilateral cervical vagotomies were performed in rats in VA/CL/SEA and VA/CLP/EA groups. Twelve hours after CLP, animals were sacrificed and liver, kidney and jejunum were harvested for evaluating the contents of tumor necrosis factor-α (TNF-α), myeloperoxidase (MPO) and diamine oxidase (DAO). The rate of water content (WCR) of the organs was determined. At the same time, the plasma levels of alanine aminotransferase (ALT) and creatinine (Cr) in each group were also detected. RESULTS: The levels of ALT and Cr in plasma, as well as TNF-α, MPO and WCR in organ tissues were markedly lower, and the activity of DAO in jejunum tissue was obviously higher than that in CLP/SEA group at 12 h after CLP (all P<0.05). The levels of ALT, Cr, TNF-α, MPO and WCR in VA/CLP/SEA group and VA/CLP/EA group were significantly higher, the activity of DAO was obviously lower than that in CLP/SEA group (all P<0.05). No statistical difference in all above measurements between VA/CLP/EA group and VA/CLP/SEA) group was observed (all P>0.05). CONCLUSION: The results indicate that EA at Zusanli point obviously decreases the levels of TNF-α in liver, kidney and jejunum tissues after CLP, and alleviates the tissue edema and dysfunction of those organs. Vagotomy decreases or eliminates the effects of EA, suggesting that activation of cholinergic anti-inflammatory pathway is one of the main mechanisms to induce the effects of EA at ST36 on CLP sepsis.

Objective To study the expression of ?-catenin and cox-2 in the bulge cells of hair follicle and investigate the relationship of their expression on cell proliferation. Methods The hair follicle was prepared from the resected cheek skin of 20 Wistar rats aged 7 days. The bulge cells were resected from the intact hair follicle and cultured in vitro. Immunocytochemical technique (ICC) was applied to detect ?-catenin and cox-2 expression in bulge cells at culture day 3, 5, 8, 13. Results ?-catenin and cox-2 strongly expressed in bulge cells and the expression correlated with the culture days. ?-catenin appeared in both plasma and nucleus, while cox-2 only in nucleus. Conclusion ?-catenin and cox-2 were correlated with bulge cells proliferation, and cox-2 might be the target gene of ?-catenin signaling pathway in nucleus.

Objective To investigate the effect of oral fluid resuscitation on circulatory oxygen dynamic parameters in dogs with burn shock.Method Eighteen male Beagle dogs were surgically prepared for the cannulation of carotid cartery and jugular vein,subjected to flame injury 50%total body surface area(TBSA)with fullthick ness 24 hours later,and they then randomly divided into 3 equal groups.The oral fluid resuscitation group (OR group)was intragastrieally injected with infusion of glucose-electrolyte solution(GES)according to parkland formula 0.5h after burn with a dose of 4mL·kg-1·% TBSA-1.Intravenous (IV) GES resuscitation group (VR group)was intravenously injected with infusion of GES as the same dose as OR group,and no-fluid resuscitation (NR)group did not receive any treatment during the first 24 hotrs.In the second 24 hours,all dogs received Ⅳ fluid resuscitation.At the end of 72 hours of injury.the mortality in each group was recorded.The mean arterial arterial pressure(MAP),hematocrit(HCT)and blood lactic acid(LAC)were determined,and blood gas analysis was evaluated for oxygen delivery(DO2),oxygen consumption(VO2)and oxygen uptake(O2ext)before injury and 2,4,8,24,48 and 72 hours after injury.Results Burn injury resulted in a 77.1%decrease in MAP,and a 48.5% increase in HCT and 533.7%increase in LAC in NR group,followed by pngressively lowering of DO2,VO2 and Oext till all animals died with in 24 hours after burn.MAP and HCT levels oftwo resuscitation groups gradually returned to the pre-injury levels within 72 hours after burn,but the LAC levels sill remained significantly higher than the pte-injury levels(P＜0.01).The MAPs of OR group were higher at corresponding intervals within 24 hours post burn than those of NR group(P＜0.01),but they were lower than those of VR group(P＜0.01).The serum LAC in OR group was markedly lowered than that in NR group,but it was higher than that in VR group.Twenty-four hours after burn injury,the DO2 level in OR group showed no significant differences compared with that of the VR group,but the levels of the VO2 and Oext were still much lower than those of VR group (P＜0.01).At the end of 72 hours,3 dogs of NR group died and none of IV group died.Caadusions Oral fluid resuscitation improves oxygen dynamic,alleviates hyperlactacidemia and reduces the mortality of animals with severe burn shock.

Objective To exPlore the effect of mammalian target of raPamycin ( mTOR ) inhibitor eVerolimus on radiosensitiVity of human non_small cell lung cancer cell line in vitro by using eVerolimus to inhibit mTOR signaling Pathway of A549. Methods Human non_small cell lung cancer cell line A549 was subjected to radiation alone or in combination with eVerolimus treatment. The 50%inhibition concentration ( IC50 ) of eVerolimus in A549 cells was detected by methylthiazol tetrazolium ( MTT) assay in vitro. EVerolimus at the 20%inhibition concentration ( IC20 ) was used to Pretreat A549 cells for 24 h. Cells were then irradiated by X_ray with 2,4,6,8 Gy. The cell surViVal fraction was comPuted by clone formation. Cell surViVal curVe was fitted by multitarget one_hit model, and mean lethal dose ( D0 ), dose quasithreshold ( Dq ), surViVal fraction at 2 Gy ( SF2 ), and sensitization enhancement ratio (SER) were calculated. The exPression ofγ_H2AX was determined by Western blotting and then the relatiVe gray Values were analyzed. Results EVerolimus significantly imProVed the sensitiVity of A549 cells to radiation. The D0 , Dq and SF2 of eVerolimus+irradiation grouP were significantly lower than those of irradiation grouP. The SER was 1. 36. The residual amount of γ_H2AX Protein in the eVerolimus + irradiation grouP was significantly higher than that of the irradiation grouP. Conclusion EVerolimus inhibiting mTOR signaling Pathway can increase the radiosensitiVity of A549 cells.