1.Clinical Observation of Simple Obesity Treated by Electroacupuncture: A Report of 80 Cases
Journal of Acupuncture and Tuina Science 2005;3(4):42-43
Eighty simple obesity patients were treated by electoacupuncturing Tianshu(ST 25), Shuidao(ST 28), Shuifen(CV 9), Guanyuan(CV 4), Daheng(SP 15), Huaroumen(ST 24),Daimai (GB 26), Quchi(LI 11), Hegu(LI 4), Liangqiu(ST 34) and Zusanli(ST 36). The results showed remarkable effect in 14 cases, middle effect in 38 cases, common effect in 23 cases and failure in 5 cases.
2.An analysis of postoperative complications in aged patients receiving pancreaticoduodenectomy
Chinese Journal of Hepatobiliary Surgery 2011;17(1):23-25
Objective To analyze the postoperative complications in aged patients receiving pancreaticoduodenectomy. Methods Pancreaticoduodenectomy was employed for the treatment of 52 aged patients. Of the 52 patients with a mean age of 70.5(65-76), 32 were male and 20 female. Postoperative pathological examination confirmed that 24 patients suffered from Vater,s ampullary and duodenal cancers, 16 from pancreatic carcinoma and 12 from cholangiocarcinoma. Results The mortality was 7.7%. Severe complications included bleeding (upper gastrointestinal tract or abdominal cavity),MOF, ARDS, postsurgical gastroparasis syndrome(PGS), mental disorder, pancreaticogastrostomy leak and biliary-enteric anastomotic leak et al. Conclusion MOF is the main lethal complication. Incidence of PGS and mental disorder is high.
3.Effects of MIP-1β and TGF-β antibody on the expansion of CD34+ cells from cord blood in stroma-contact culture system
Yingli WU ; Yijun ZHANG ; Linsheng ZHANG
Journal of Third Military Medical University 2001;23(3):352-355
Objective To study the effects of MIP-1β and TGF-β antibody on CD34+ cells from cord blood in stroma-contact culture system. Methods Immunomagnetic selected CD34+ cells were inoculated onto the pre-established irradiated human stroma layer. MIP-1β, TGF-β antibody (20 μg/ml) and MIP-1β+anti-TGF-β (5 μg/ml) were added on day 0 and day 5. On day 5 and day 10, cell counting, hematopoietic progenitor cells count were made by semi-solid culture and CD34+ cells were assayed by FACS. Results On day 5, no significant difference of total cell number was observed as compared with the control group (P>0.05), but the number of CD34+cells, CFC, and HPP-CFC in TGF-β antibody and MIP-1β+ TGF-β antibody groups was significantly higher than that in the control (P<0.05). On day 10, the number of total cell, CD34+ cells, CFC, and HPP-CFC in TGF-β antibody and MIP-1β+ TGF-β antibody groups was significantly higher than that in the control (P<0.05). No significant difference was observed between groups MIP-1β and control either on day 5 or day 10 (P>0.05). Conclusion MIP-1β has no significant effect on the CD34+ cells as compared with the control while the CD34+ cells can be expanded 1-3 folds with TGF-β antibody (20 μg/ml) or MIP-1β+ TGF-β antibody (5 μg/ml) in 10 days. There are synergic interactions between MIP-1β and TGF-β antibody.
4.Processing method, extraction technology and quality evaluation analysis of Paeonialactiflora Pall
Bijuan WANG ; Yijun ZHAO ; Haiqun ZHANG
Chinese Journal of Biochemical Pharmaceutics 2017;37(2):43-45
Objective To study the processing method,extraction technology and quality evaluation analysis of Paeonialactiflora Pall, so as to provide reference for selecting the best method and improving its quality .Methods One year and 3 years Chinese herbal medicine Paeonialactiflora Pall was used.The use of raw materials and cooked products for processing methods to paeoniflorinas the representative , and the content was determined by high performance liquid chromatography.With the optimization of orthogonal test, the methods for the determination of effective components of radix paeoniaealba were summarized and the quality control methods were analyzed .Results The content of paeoniflorin in raw paeonialactiflora pall was (6.33 ±0.52)%,and the content of paeoniflorinin cooked Paeonialactiflora Pall was (5.74 ±0.45)%, which was with statistical significance(P <0.05).The content of paeoniflorin in raw paeonialactiflora pall was (5.90 ±0.38)%,and 4.85 ±0.45% in three years cooked paeonialactiflora pall, and the differenceswere with statistical significance(P<0.05).Extracted three times with 70% ethanol, time 65 min, 6 times the amount of solvent,the paeoniflorin high cream rate was highest.By means of induction, several active ingredients should be screened out to control the content of paeonialactiflora pall to publish multiple indicatorsquality control methods .Conclusion The preparation method of Chinese herb paeonialactiflora pall has showed that the active ingredient content is morein raw herb.To establish quality control method of multiple indicators of paeonialactiflora pall based on raw herb,gradually improve the quality of more comprehensive paeonialactiflora pall quality control model.To optimize the extraction of Chinese herbal medicine,provide scientific reference for the sustainable utilization and industrial production of paeonialactiflorapall.
5.Therapeutic Effect Evaluation of Arsenious Acid-FOLFOX4 Combined Chemotherapy in Metastatic Liver Cancer
Yijun ZHANG ; Yongqiang WENG ; Jigang QIU
Chinese Journal of Bases and Clinics in General Surgery 2003;0(04):-
0.05),but the benefit was significantly different (P
6.X-ray diagnosis of stress fracture of the pubic ramus
Changbin QUAN ; Kun YOU ; Yijun ZHANG
Chinese Journal of Radiology 2000;0(11):-
Objective To study the X-ray findings and evaluate the value of X-ray in diagnosis of stress fracture of the pubic ramus. Methods The X-ray findings of 66 cases (18 males and 48 females, ranging in age from 18-21 years, mean age 19.6 years) with stress fracture of the pubic ramus were retrospectively analyzed. Fifty-five cases were military recruits undergoing basic training, and 11 army veterans were undergoing intensive goose step training. Results Sixty-two cases had stress fractures of the inferior pubic ramus including right-sided in 26, left-sided in 30, and bilateral in 6. Four had ipsilateral inferior and superior pubic rami fractures. With 2 weeks after onset, the radiographs were normal in 3 cases. The 66 cases had either a slight transverse fissure (34 cases) or a small dense callus (65 cases) or both during the examination period of 4 weeks to 8 months. Then the calcium resorbed at the margins of the fracture and at the same time the callus increased and surrounded the bone. Finally, the resorptive zone disappeared and the callus became homogeneous. This callus formation persisted for several months and disappeared gradually. Conclusion X-ray examination is the first imaging method of choice for detecting stress fracture of the pubic ramus. With combining clinical data, correct diagnosis can be made in the majority.
7.Construction of the fusion protein between EGFP and IgG-binding peptide
Hongming YANG ; Yijun ZHANG ; Peng GAO
Chinese Journal of Marine Drugs 2001;0(05):-
Objective Making the fusion protein of IgG-binding peptide with enhanced green fluorescent protein(EGFP) and determining its bioactivity.Methods The enhanced green fluorescent protein(EGFP) gene was cloned into pEZZ 18 vector containing ZZ peptide gene to construct expression vector pSpA-EGFP-His.The fusion protein was expressed in E.coliDH5? and its bioactivity was detected by competitive ELISA and fluorescence properties.Results The fusion protein migrated at approximately 42kD in SDS-PAGE,which correspond to the theoretical molecular weight.The spectra of SpA-EGFP fusion protein was similar to what was reported.SpA-EGFP competed with SpA-Peroxidase to bind IgG.Conclusion The plasmid pSpA-EGFP-His correctly expressed in E.coli.The fusion protein retains the bifunctional effects of EGFP and IgG-binding activity.
8.Expression and function of plasminogen activator inhibitor-3 in normal adult skin
Cheng ZHANG ; Tian YANG ; Yijun ZENG
Journal of Third Military Medical University 2003;0(08):-
Objective To explore the expression of plasminogen activator inhibitor-3 (PAI-3) in normal adult skin. Methods Immunohistochemistry and RT-PCR were used to detect the expressions of PAI-3 and uPA. Results mRNA of PAI-3 and uPA were detected. PAI-3 was mainly expressed in stratum basale, stratum spinosum and stratum granulosum, and its expression enhanced in the well differentiated keratinocytes of the epidermis. uPA was located in basal layer. Conclusion PAI-3 exists in normal adult skin and is related with the differentiation of epidermal keratinocytes.
9.Mesenchymal stem cells-stents complex in repair of meniscus injury in knee joint
Yijun LIU ; Qiuxia ZHANG ; Jing TIAN
Chinese Journal of Tissue Engineering Research 2014;(7):1088-1093
BACKGROUND:Meniscus injury is a common disease, whose curative effect is not obvious. The clinical application of tissue engineering technology is gradual y rising, and mesenchymal stem cells-stents complex is expected to be used for the treatment of meniscus injury.
OBJECTIVE:To review the application process of mesenchymal stem cells-stents complex in meniscus injury, in order to provide reference for further study and clinical practice.
METHODS:A computer-based online search of PubMed database, VIP database, Wanfang database and China National Knowledge Infrastructure between 1980 and 2013 was performed to search related articles on mesenchymal stem cells-stents complex in meniscus injury. The key words were“mesenchymal stem cell, meniscus injury, tissue engineering, scaffold, stent”in English and“mesenchymal stem cell, meniscus injury, tissue engineering”in Chinese. For the articles in the same field, we preferred the ones published recently or in authorize journals. Meta-analysis and repetitive studies were excluded. 282 articles were included, and final y 59 articles were included according to inclusion criteria.
RESULTS AND CONCLUSION:Nowadays, numerous experiments showed that mesenchymal stem cells could differentiate into chondrocytes both in vivo or vitro. Mesenchymal stem cells-stents complex could be effective to meniscus injury. Although this technique is stil in exploratory stage, it has promising application prospects in treatment of meniscus injury, because it is characterized by convenience to col ect samples, vitality and no antigenicity.
10.Design and application of exposure remoter of mobile X-ray machine
Lianqiang ZHANG ; Yaoliang XU ; Yijun ZOU
Chinese Medical Equipment Journal 2004;0(08):-
Mobile X-ray system is a very important equipment in hospital,but it's difficult to protect the user from the exposure of the X-ray while the equipment is in use.This paper introduces a circuit that is able to control the exposure by remote controller and it can solve the problem of the protection better.The efficiency is proved to be good by experiments.