Objective To investigate risk factors of hypertension for urban residents in Zunyi city ,and to provide a useful theo-retical support to the firstly prevention of hypertension in this area .Methods A case-control study was used meanwhile samples were selected by stratified sampling method .chi-square analyse and Logistic regression analyse were used to analyse the data .Re-sults 555 pieces of effective files were collected ,including 326 hypertension files and 229 health files .Single factor analysis showed that 12 factors were meaningful to the morbidity of hypertension in people living in Zunyi city (P< 0 .05) ,Multi-factor uncondi-tioned Logistic regression analysis showed that there were 6 independent factors as age ,hyperlipidemia ,diabetes mellitus ,abdominal obesity ,high salt diet ,family history of hypertension(P<0 .05) .Conclusion In preventing people in Zunyi city from falling to hy-pertension ,ministries might particularly stronger the propaganda and education in people who has those risk factors so that we can control steerable factors on purpose ,and then to prevent complications that may caused by hypertension .

Quantum dots (QDs) are novel photo-stable semiconductor nanocrystals with wide excitation spectra and narrow, symmetrical emission spectra. QDs can be used as molecular probes by conjugating to a wide range of biological targets, including proteins, peptides and nucleic acids. It has been widely used in bio-labeling, fast detection and biological imaging. In this review, we focus on the applications of QDs in tissue engineering and its potential bio-safety issues.
Diagnostic Imaging ; Humans ; Molecular Probes ; Nucleic Acids ; Peptides ; Proteins ; Quantum Dots ; Tissue Engineering

BACKGROUND:Light-cured composite resin is used for dental crown defective repair in terms of both function and morphology.
OBJECTIVE:To investigate the therapeutic effects of light-cured composite resins on crown defects.
METHODS:Relevant literatures concerning light-cured composite resins for repair of crown defects were retrieved. Literatures which met the study standard were deeply analyzed. Meanwhile, fol ow-up results and literature results were compared for patients receiving light-cured composite resins for repair of crown defects to identify the feasibility of light-cured composite resins for repair of crown defects.
RESULTS AND CONCLUSION:Based on the included data, the successful rate of light-cured composite resins for repair of crown defects was over 90%. Clinical fol ow-up of 12 patients undergoing light-cured composite resin showed that the repaired crowns were stable and fixed, and exhibited a symmetrical appearance with the contralateral namesake tooth of the adjacent tooth, and there was a normal bite relationship. During the fol ow-up study, 19 teeth repaired from 12 patients had no loosing, no shedding, no percussion pain and normal bite relationship. X-ray films showed no apical shadows. Light-cured effects were limited to the upper and middle parts but not to the apical part. Therefore, a better therapeutic effect can be obtained.

Objective To investigate and evaluate the clinical effect of the application of medical adhesive in limited septoplasty by endoscope.Methods 57 patients were selected to undergo operation of limited septoplasty by endoscope.The patients were divided into packing group and bonding group.The packing group had 29 patients,whose nasal cavity was packed with vaseline gauze.While the bonding group had 28 patients,their nasal septum mucosa was adhesive fixed by medical adhesive,and the nasal cavity without packing.The indexes included the cardinal symptoms(nasal pain and headach,sleep status),signs (seepage amount of bleeding,mucosal edema),degree of discomfort in nasal cavity processing after operation(pain and bleeding) and length of stay were compared between the two groups.Results The above indexes in the bonding group were apparently lightened than the packing group and the length of stay was shortened.The differences were statistically significant (all P ＜ 0.05).Conclusion In operation of limited septoplasty by endoscope,the using of medical adhesive replacing nasalpacking could reduce the trauma on patients by operation and lighten the pain.The curative effect is satisfactory and it has excellent clinical application value.

AIM: To study the alterations of heme oxygenase-1 mRNA in vascular smooth muscle cells(VSMC) induced by lipopolysaccharide(LPS) and the role of heme oxygenase(HO)/carbon monoxide(CO)pathway in the disorders of regulation of cardiovascular system by LPS. METHODS: LPS (final concentrations 10 mg/L,30 mg/L and 50 mg/L) was added in cultured VSMCs for 6 h respectively or 10 mg/L LPS for 9 h and 18 h. MDA content, LDH release and the rate of trypan blue uptake of VSMC were measured. HO-1 mRNA expression was examined by Northern Blot. RESULTS: VSMC HO-1 mRNA expression was increased gradually with the increasing of LPS concentration. When final concentration of LPS was 50 mg/L, the HO-1 mRNA expression of VSMC was increased by 176.7% compared with control. When LPS final concentration was 10 mg/L, the HO-1 mRNA expression increased gradually along with the culture time. When cultured for 18 h, the HO-1 mRNA expression of VSMC was increased by 195.6% compared with control. Only at LPS 50 mg/L for 6 h and 10 mg/L for 18 h, the rate of trypan blue uptake，MDA content and LDH release were significantly increased. CONCLUSION: LPS can induce the HO-1 mRNA expression of VSMC and that were dose-dependent and time-dependent. The inducible HO may play an important role in the pathogenesis of vascular system under LPS.

Objective To evaluate the clinical characteristics and surgical risks for elderly patients with gastric cancer. Methods The clinical data from 607 patients, who received surgical therapy for gastric cancer between January 2006 and December 2009, were retrospectively analyzed.Among them, 267 patients were over 65 years of age, and the other 340 patients (≤65 years of age)were served as control. Complete surgical resection was done by doctors in same group. The preoperative complications such as cardiopulmonary disease and post-operative complications as well as pathologic patterns were compared between two groups. Results Coexisted diseases were found more in elderly patients than in controls (68.91% vs 31.63 %, P＜0.01). Whereas there was no significant difference between two groups with respect to post-operative complication [28. 19% (75/267)vs 25.59 % (87/340)]. However, the incidence of cardiopulmonary complications, especially pulmonary infection, was higher in elderly patients than in controls (17.23% vs 5.29% ,P＜0.01). The digestive tract complications were related to the pattern of radical operation. Post-operative complication occurred less in patients treated with D1 radical operation than those with D2 and D3 radical operation.Conclusions The incidence of post-operative complication in elderly patients with gastric cancer was closely related to the surgical method. The elderly patients may has less complications if they were operated with D1 radical method which can reduce risk of complication and improve the quality of life.

AIM: To study the alterations of heme oxygenase-1 mRNA in vascular smooth muscle cells(VSMC) induced by lipopolysaccharide(LPS) and the role of heme oxygenase(HO)/carbon monoxide(CO)pathway in the disorders of regulation of cardiovascular system by LPS. METHODS: LPS (final concentrations 10 mg/L,30 mg/L and 50 mg/L) was added in cultured VSMCs for 6 h respectively or 10 mg/L LPS for 9 h and 18 h. MDA content, LDH release and the rate of trypan blue uptake of VSMC were measured. HO-1 mRNA expression was examined by Northern Blot. RESULTS: VSMC HO-1 mRNA expression was increased gradually with the increasing of LPS concentration. When final concentration of LPS was 50 mg/L, the HO-1 mRNA expression of VSMC was increased by 176.7% compared with control. When LPS final concentration was 10 mg/L, the HO-1 mRNA expression increased gradually along with the culture time. When cultured for 18 h, the HO-1 mRNA expression of VSMC was increased by 195.6% compared with control. Only at LPS 50 mg/L for 6 h and 10 mg/L for 18 h, the rate of trypan blue uptake,MDA content and LDH release were significantly increased. CONCLUSION: LPS can induce the HO-1 mRNA expression of VSMC and that were dose-dependent and time-dependent. The inducible HO may play an important role in the pathogenesis of vascular system under LPS. [

Objective To investigate the intervening effect of movable cupping on sub-health status. Methods Movable cupping was performed on bilateral bladder meridians and the Du meridian in 12 patients with sub-health status. The sub-health status rating scale score, the visual analogue pain intensity scale score, hemorheological parameters and immunoglobulins were observed before and after movable cupping.Results The sub-health status rating scale score, the visual analogue pain intensity scale score, whole blood viscosity 200 and plasma viscosity decreased after treatment compared with before in the patients, and there were statistically significant differences (P<0.05). Whole blood viscosity 1, whole blood viscosity 2 and whole blood viscosity 3 decreased and IgG, IgA and IgM increased after treatment compared with before in the patients, but there were no statistically significant differences (P>0.05). Conclusion Movable cupping has clinically a certain improving effect on bodily sub-health status.

OBJECTIVE To explore the application value of the cortical bone packing in external auditory bony canal and attic reconstruction in tympanoplasty.METHODS A total of 30 patients admitted during the period of middle ear cholesteatoma patients in our hospital from March 2014 to March 2016,were randomly divided to observation group and control group,the radical mastoidectomy with canal wall down was adopted in the control group,the radical mastoidectomy with canal wall down combined with bone autograft in the external auditory meatus and bony powder mastoid obliteration in observation group,the postoperative out-come between two groups were compared.RESULTS There was no significant difference between the speech frequency average air bone conduction and air conduction threshold before the treatment in two groups.There was significant difference between the speech frequency average air bone conduction and air conduction threshold after the treatment(P＜0.05),the air conduction threshold significantly reduced,the patients in the observation group were significantly lower than the control group,there was no recurrence of cholesteatoma in two groups of patients before and after the treatment during follow-up period;the dry ear time in observation group was significantly lower than that of the control group,and dry ear the incidence was significantly higher than that of the control group,the difference is statistically significant(t=2.758,4.865,P＜0.05).CONCLUSION Radical mastoidectomy with canal wall down combined with the cortical bone packing in external auditory bony canal and attic reconstruction,can effectively presesre the patient hearing,it should be recommended.

OBJECTIVETo evaluate the clinical efficacy of YANG's pricking-cupping therapy for knee osteoar thritis (KOA). Methods This was a multi-center randomized parallel controlled trial. One hundred and seventy one patients with KOA were randomly allocated to a pricking-cupping group (89 cases) and a conventional acu puncture group (82 cases). Neixiyan (EX-LE 4), Dubi (ST 35) and ashi points were selected in the two groups. Patients in the pricking-cupping group were treated with YANG's pricking-cupping therapy; the seven-star needles were used to perform pricking at acupoints, then cupping was used until slight bleeding was observed. Patients in the conventional acupuncture group were treated with semi-standardized filiform needle therapy. The treatment was given for 4 weeks (from a minimum of 5 times to a maximum of 10 times). The follow-up visit was 4 weeks. The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and the visual analogue scale (VAS) were adopted for the efficacy assessments.

RESULTSThe pain score, stiffness score, physical function score and total score of WOMAC were all reduced after 4-week treatment and during follow-up visit in the two groups (all P<0. 0001). Except that the difference of stiffness score between the two groups was not significant after 4-week treatment (P>0. 05), each score and total score of WOMAC in the pricking-cupping group were lower than those in the conventional acupuncture group after 4-week treatment and during follow-up visit (P<0. 0001, P<0. 01). After 2-week treatment, 4-week treatment and during follow-up visit, the VAS was all reduced compared with that before treatment (all P<0. 0001) ; with the increase of the treatment, the reducing trend of VAS was more significant (P<0. 0001). The scores of VAS in the pricking-cupping group were lower than those in the conventional acupuncture group after 4-week treatment and during follow-up visit (P < 0. 01, P <0. 0001). CONCLUSION The YANG's pricking-cupping and conventional acupuncture therapy can both significantly improve knee joint pain and function in patients with KOA, which are relatively safe. The pricking cupping therapy is superior to conventional acupuncture with the identical selection of acupoints.

Acupuncture Therapy ; Adult ; Aged ; Arthralgia ; physiopathology ; therapy ; Combined Modality Therapy ; Female ; Humans ; Knee Joint ; physiopathology ; Male ; Medicine, Chinese Traditional ; methods ; Middle Aged ; Osteoarthritis, Knee ; physiopathology ; therapy ; Treatment Outcome