Wheezing is one of the most common respiratory symptoms in infant,which caused by diseases including bronchiolitis,interstitial pneumonia,asthma,etc.The infection of virus is the main and most important factor inducing wheezing in infants,the role of bacteria is not clear yet.In recent years,some researches found that bacteria usually colony and infect in the respiratory of children with asthmatic disease that plays important role of the wheezing prognosis in the early neonatal,but this is still controversial.So this article will overview researches of the role of bacterial colonization/infection in wheezing illness in recent years.

Objective:To understand the prevalence of caries in the children aged 12 years in Fujian Province and to compare the influence of two kinds of dental probes examinations on caries detect rate.Methods:Based on oral health investigation of WHO and national oral health survey standards,multi-stage stratified sampling was used to randomly select 792 children in Fujian province to accept examination separately by two dental probes.Results:The prevalence of dental caries of youth aged 12 years in Fujian province has no statistical significance in different gender or between city and countryside.But the caries prevalence of female was higher than that of male when used by sharp dental probe.About 23.23% was found in different detection rates when using two dental probes.Compared to the sampling investigation of Fujian province in 2000 year,the caries prevalence has no obvious increase.Conclusion:The sharp probe has higher detection rate than CPI probe.

Objective To investigate the effect of insulin glaring(IG)and neutral protamine hagedorn(NPH)on metabolic control and islet cell function in type 2 diabetic patients who secondly failed to sulfonylureas(SU)treatment.Methods Fifty-two diabetic patients with second SU failure were studied in our hospital during May,2004 to Nov,2004,and they were divided into two groups:twenty treated with glipizide extended release tablets(GERT)plus IG injection,while thirty-two with GERT plus NPH.Fasting and postprandial blood glucose,HbA_1c,fasting and postprandial C-peptide were determined in all patients at different following points.Results Blood glucose decreased obviously and similarly in two groups;however,hypoglycemia occurred less in IG group.Interestingly,fasting and postprandial C-peptide significantly increased in IG group,but didn't change in NPH group.Conclusion Combination treatment with IG or NPH has good effect on metabolic control in second SU failure patients;and moreover,IG improves B-cell function.

Objective: To compare the influence of two explorers on the enamel of artificial initial caries. Methods: Built an artificial initial caries model in vitro. Compared the influence of two explorers on the enamel of artificial initial caries. Results: 1.The holes probed by sharp explorer were deep and narrow. The holes probed by CPI explorer were shallow and wide. 2. The average strain energy produced by CPI explorer was larger than sharp explorer(Z=-2.021,P=0.043), But there was no significant difference because the strain energy produced by two explorers were in the same rate range. Conclusion: The damage on the enamel of artificial initial caries caused by sharp explorer is not larger than that caused by CPI explorer. So we suggest that the sharp explorer can still be used in sampling survey and clinical examination. But a gentle probing action is strongly recommended.

A magnetoelastic Staphylococcus aureus (S.aureus) wireless sensor was fabricated to detect S.aureus in different liquid medium.After 0.2 mL bacteria with proper concentration was taken into the tes ting cuvette containing 2 mL liquid medium, the resonance frequency of magnetoelastic sensor changed with the bacteria growth.Via changing the concentration of beef extract and peptone, sensor had the most frequen cy response in culture medium CM2-2 incubated with 2 × 10~5 cells/mL S.aureus.The results indicated that this sensor can be used to determine S.aureus concentrations of 3 × 10~3 to 2×10~7 cells/mL in culture medium and 10~4 to 10~7 cells/mL in milk, with the detection limits of 10~3 cells/mL and 104 cells/mL, respectively.The sensor frequency shift was correlated to the logarithmic value of the S.aureus concentration, and the correlation coefficient was 0.98 in milk and 0.99 in culture medium.

Objective: To explore the clinical efficacy of a new drug for acute coronary syndrome (ACS), ticagrelor in treating the patients with acute ST-segment elevation myocardial infarction (STEMI), and to compare the effect between ticagrelor and clopidogrel in order to obtain the prospective information of ticagrelor in clinical practice.
Methods: A total of 307 STEMI patients were divided into 2 groups, Treatment group, the patients received ticagrelor with aspirin, n=157 and Control group, the patients received clopidogrel with aspirin, n=150. With a period of treatment, the major adverse cardiac events (MACE), rate of thrombosis re-formation and routine blood test result were compared between 2 groups.
Results: Treatment group presented the better LVDD, LVEF, platelet level and ST-segment recovery than those in Control group, P<0.05 or P<0.01. Treatment group showed slightly higher rate of side effect such as dififcult breathing and bleeding, while those reaction did not affect the treatment or special management for the patients.
Conclusion: The clinical effect of ticagrelor is similar to clopidogre for improving the platelet level and cardiac function in STEMI patients, while the clinical advantage reported in abroad should be further studied.

Diabetic patients with secondary sulfonylureas failure were shifted to glipizide plus glargine ( n = 20) or neutral protamine hagedorn (n =32). Sixteen weeks later, blood glucose and HbAIC were decreased and serum C peptide level increased in both groups. Hypoglycemia occurred less frequently and?-cell function was improved in glargine group.

Objective To detect infection of Angiostrongylus cantonensis and examine effection of treatment to prepare monoclonal antibodies(McAbs). Methods Six-week-old BALB/c mice were imrnunized by the intraperitoneal injection of e/s antigens of Angiostrongylus cantonensis. Fusion of splecn cells from immunized mice with prepared SP2/0-Ag14 myeloma cells was performed in RPMI 1640. Fused cells were suspended in RPMI 1640 containing 1% HAT and 20% fetal calf serum and dispensed into 96-well cell culture plates. The supernatants of clones were screened by ELISA with sera of patients of angiostrongyliasis.Distribution of cohere antigen of 12D5 and 21B7 monoclonal antibodies was analyzed with immunohistochemistry. Two McAbs ( 12D5 and 21B7) were applied to detect the circulating antigen (CAg) in the sera of rats infected with A. cantonensis and angiostrongyliasis patients respectively by double antibody sandwich ELISA.Results 12D5 McAb was identified as IgG1 and 21 B7 McAb was IgM. Western blot result showed two McAbs could used to identified 55 × 103 protein of adult worms of A. cantonensis. Cohere antigen of 12D5 and 21B7 monoclonal antibodies were distributed on intestine surface of A. cantonensis. The detection rates of CAg in the sera of infected rats 100% (48/48), the detection rates of CAg in the sera of angiostrongyliasis patients was 100% (32/32). No cross-reaction to sera of patients with other infection of parasites, such as clonochiasis, fasiolopsiasis, ancylostomiasis, trichinosis, anisakiasis as well as schsitosomiasis, and health srea did not reacted with 12D5 and 21B7 McAbs,and detaction rate of antibody of angiostrongyliasis patients only reached 75% (24/32) with antigen of A. cantonensis. Conclusion Cohere antigen of 12D5 and 21B7monoclonal antibodies were antigens of enteric epithelium. Sandwich ELISA with 12D5 and 21B7 McAbs showed high specificity act as detecting CAg of A. cantonensis in sera of infection animal and patients. It is apparent that Sandwich ELISA with 12D5 and 21 B7 is not only rapid and simple without requirement of special instrument, but also rather sensitive and specific for the detection of current infection with A. cantonensis.

Objective To explore the characteristics of attenuated plaque (AP) in culprit lesions for acute coronary syndrome (ACS) in elderly patients.Methods This study included 166 ACS patients meeting the conditions from Jan.1, 2013 to Sep.31, 2014.Clinical data, vascular lesions determined by coronary angiography and intravenous ultrasound (IVUS)-demonstrated characteristics of culprit plaque, which included presence of AP, maximum attenuation arc, extravascular elastic membrane area, lumen area, plaque area, plaque burden and remodeling index, were recorded.Results Compared with non-elderly group, elderly group had a higher proportion of multiple coronary-artery lesions (50.0％ vs.35.1％, x2=6.525, P =0.038), more attenuation plaques (62.0 ％ vs.45.9％, x2 =4.245, P=0.039), a larger maximum attenuation arc[(142±80)° vs.(115±54)°, t=5.254, P=0.000], larger plaque area[(14.2±3.9)mm2 vs.(12.3 ± 4.1) mm2, t=2.325, P=0.022], more plaque burden [(81.1±14.2)％ vs.(76.4±13.5)％, t=2.025, P=0.042] and higher remodeling index [(1.19±0.17) vs.(1.09±0.13), t=4.245, P=0.031].Conclusions The onset of ACS is more closely related with the unstable plaque in elderly patients, and strengthening the measures for plaque stabilization will be helpful in the prevention and treatment of ACS in the elderly.

Objective To identify the species of Biomphalaria snails collected in Shenzhen reservoir,based on the mitochondrial 16S rDNA sequences.Methods The 16S rDNA fragments were amplified by PCR from the genome DNA of Biomphalaria snails,and inserted in plasmid pMD-18T for sequencing.The sequence of 16S rDNA fragment and its phylogenetic relationships with those of other species of Biomphalaria snails were analyzed with BLAST and MEGA4 software.Results The amplified 16S rDNA fragment of the Biomphalaria snails was about 466 bp in length.As aligned with the corresponding sequences of the related Biomphalaria species,the identity of nucleotides was 99％ with 1 isolate of Biomphaltria straminea (B.straminea),98％ with 3 isolates of B.kuhniana,95％ with 1 isolate of B.intermedia,and 94％ with 1 isolate of B.edisoni.Based on the 16S rDNA sequence,the results of phylogenetic analysis with neighbor-joining (NJ) and unweighted pair-group method with arithmetic means (UPGMA) indicated that the snails had close genetic relationships with the B.straminea isolate (Genbank accession NO.AY030213.1) Conclusion The Biomphalaria snails collected in Shenzhen reservoir could be classified as B.straminea based on the characteristics of 16S rDNA sequence.