Objective To explore the correlative factors of sudden death in patients suspected with pulmonary thromboembolism (PTE) in emergency room (ER).Methods A retrospective analysis was conducted.The clinical data of 12 patients with sudden death suspected with PTE (sudden death group) in ER of the Air Force General Hospital from January 2011 to June 2014 were analyzed.The non-sudden death group included 35 patients during the same time period who were diagnosed with PTE based on findings of CT pulmonary arteriography (CTPA) and showed no sudden death in ER.Factors,including sex,age,previous operation,tumor,syncope,dyspnea,bilateral or unilateral edema of lower extremity,heart rate (HR),white blood cell count (WBC),D-dimer,arterial partial pressure of oxygen (PaO2),arterial partial pressure of carbon dioxide (PaCO2) and typical clinical manifestation of electrocardiogram (SⅠTⅢQⅢ),were compared between the two groups.The potential predictors of sudden death of PTE were analyzed by logistic regression analysis.Results Young age (years old:51.3±15.5 vs.62.3±14.4),lower PaO2 [mmHg (1 mmHg =0.133 kPa):49.9± 12.3 vs.62.7± 10.2],higher HR (bpm:122.0± 19.5 vs.89.1 ± 18.5) and higher WBC (× 109/L:13.8 ± 6.9 vs.7.2 ± 2.5) were found in sudden death group as compared with those in non-sudden death group (P ＜ 0.05 or P ＜ 0.01).There was no significant differences in D-dimer level and PaCO2 between sudden death group and non-sudden death group [D-dimer (pg/L):986 (891,3 230) vs.2089 (598,3 397),PaCO2 (mmHg):33.0 (28.6,43.4)vs.36.5 (32.9,41.0),both P ＞ 0.05].The syncope,antineoplaston treatment or tumor metastasis within 6 months,operation in previous 4 months,bilateral asymmetrical edema in sudden death group were more than those of the non-sudden death group,and chest pain was less (P ＜ 0.05 or P ＜ 0.01).Difference in gender,dyspnea and typical SⅠTⅢQⅢ in electrocardiogram were not significant between the two groups (all P ＞ 0.05).It was shown by multiple logistic regression analysis that higher HR [odds ratio (OR) =1.124,95％ confidence interval (95％CI) =1.024-1.235,P =0.014] and higher WBC (OR =1.347,95％CI =1.043-1.738,P =0.022) were identified as independent risk factors of sudden death for PTE.Conclusions Gender,dyspnea,typical S Ⅰ TⅢQⅢ in electrocardiogram,PaCO2 and D-dimer seem unrelated to sudden death of patients with PTE.Young age,chest pain,syncope,bilateral asymmetrical edema,antineoplaston treatment or tumor metastasis within 6 months,operation in previous 4 months and low PaO2 were potential predictors of sudden death according to the univariate analysis.Higher WBC and higher HR are independent risk factors of sudden death for PTE patients.

OBJECTIVETo evaluate the postoperative complications of microscopic and conventional Palomo varicocelectomy in the treatment of varicocele in army personnel.

METHODSA total of 260 army personnel with varicocele were randomized to receive microscopic varicocelectomy (group A, n=130) and conventional Palomo varicocelectomy (group B, n=130). The postoperative recurrence and complications (scrotal edema, testicular pain and testicular atrophy) were compared between the two groups.

RESULTSAfter 1 year of follow-up, the recurrence rates in groups A and B were statistically comparable (5.3% vs 3.8%, P>0.05). The incidences of testicular atrophy and scrotal edema were significantly lower in group A than in group B (0.7% vs 3.1%, P<0.05; 3.1% vs 14.6%, P<0.05), and the rate of testicular pain relief was significantly higher in group A (90.7% vs 67.7%, P<0.05).

CONCLUSIONMicroscopic varicocelectomy can be a good choice in the treatment of varicocele in army personnel.

Adolescent ; Adult ; Groin ; surgery ; Humans ; Male ; Microsurgery ; adverse effects ; methods ; Postoperative Complications ; Treatment Outcome ; Urogenital Surgical Procedures ; adverse effects ; Varicocele ; surgery ; Young Adult

Objective To investigate the effects of long-term exposure to three new types of light emitting diode (LED) sources on the behavior, learning, and memory of rats. Methods A total of 160 specific pathogen-free SD rats were divided into eight groups as followed, trichromatic fluorescent lamps color temperature control group, violet-chip full-spectrum white LED group, blue-chip white LED group, and blue-chip full-spectrum white LED group based on the light sources types, with color temperature of 4 000 K and 6 500 K groups in each group using the 4×2 factorial design. There were 20 rats in each group, with half of the rats were males and half females. Rats were exposed to artificial lighting, and the illumination was set at 750 lx. The rats in each group were exposed to different lighting environments for 12 hours per day for 24 weeks. The open-field and step-down tests were conducted in rats after 24 weeks exposure, followed by sacrifice of rats and measurement of organ coefficients. Differences in body weight, organ coefficients, and neurobehavioral indexes of rats in different groups were compared. Results The spleen coefficient of female rats decreased in blue-chip white LED of 6 500 K color temperature group, and the liver coefficient of male rats decreased in the violet-chip full-spectrum white LED of 4 000 K color temperature, blue-chip full-spectrum white LED of 4 000 K color temperature, and blue-chip full-spectrum white LED of 6 500 K color temperature groups, compared with the same-sex rats in trichromatic fluorescent lamps with same-color temperature control group (all P<0.05). The result of different types of light sources compared in the open-field test showed that the index of total distance and movement speed of female rats in the blue-chip full-spectrum white LED group were lower than those in the other three groups, and the time cost to the central area was longer than that in the blue-chip white LED group and the violet-chip full-spectrum white LED group (all P<0.05). The total distance and movement speed of male rats in the blue-chip full-spectrum white LED group were longer or higher than those in the violet-chip full-spectrum white LED group (all P<0.05). Based on the comparison of color temperature, the time and total distance of male rats in 6 500 K color temperature group were lower than that in the 4 000 K color temperature group (both P<0.05). In the step-down test, both male and female rats in the blue-chip full-spectrum white LED group made more errors compared with other three groups with the same gender (all P<0.05). Conclusion Based on the experimental conditions of this study, the blue-chip full-spectrum white light LED affects behavior, learning and memory of the rats, and trichromatic fluorescent lamp has the lowest effect on neurobehavior. The color temperature also affects behavior of the rats, and high color temperature has higher risk.

Objective To study the factors influencing prognosis in the patients with recurrent glioblastoma muhiforme (GBM) and to investigate the effect of retreatemt.Methods The retrospective analysis method was adopted to collect the clinical and follow up data in 36 cases of recurrent GBM retreatment in the neurosurgery department of this hospital from March 2008 to March 2013.The prognosis influencing factors were analyzed.Results The univariate analysis results showed that the gender,resection degree,treatment mode and initial scheme had the influence on the progression free survival(P＜0.05).The resection degree had an impact on the overall survival(P＜0.05).The multivariate analysis results showed that KPS score,resection degree and treatment mode had effect on the progression free survival(P＜0.05).The resection degree had an influence on the overall survival (P＜0.05).Conclusion If the patients with recurrent GBM still hasthe chance of operation whole excision,the re-treatment can reach the effect for relieving the symptoms,improving the quality of life and prolonging the survival period.

Objective To analyze the relationships of the expression of retinoid X receptor alpha(RXRA)and nuclear receptor interaction protein 1(NRIP1)in colorectal cancer tissues with the fea-tures of clinical pathology and prognosis.Methods A total of 106 specimens from colorectal cancer tissues of patients during surgery were included in colorectal cancer group(n=106),and corre-sponding paracancer tissue specimens were included in paracancer group(n=106).The expressions of RXRA and NRIP1 in colorectal cancer tissues were detected by immunohistochemistry.Multivariate Cox regression analysis was used to investigate th4e effects of RXRA and NRIP1 expressions on the prognosis of patients with colorectal cancer.Results The positive expression rates of RXRA and NRIP1 in colorectal cancer group were 66.04％,69.81％,which were significantly higher than 33.96％and 30.19％in the para-cancer group(P＜0.05).The positive expression rates of RXRA and NRIP1 in patients with pathological stage Ⅲ,low differentiation,serous infiltration and lymph node metastasis were higher than those in patients with pathological stage Ⅱ,medium and high differ-entiation,no serous infiltration and lymph node metastasis(P＜0.05).The 3-year overall survival rates of patients with pathological stage Ⅱ,low differentiation,no serous infiltration,no lymph node metastasis,negative RXRA and NRIP1 were higher than those of patients with pathological stage Ⅲ,medium and high differentiation,serous infiltration,lymph node metastasis,positive RXRA and NRIP1(P＜0.05).Multivariate Cox regression analysis showed serous membrane infiltration(HR=2.687;95％CI,1.531 to 3.156),positive RXRA(HR=3.743;95％CI,2.217 to 5.992),positive NRIP1(HR=2.641;95％CI,1.124 to 4.757)were influencing factors for prognosis in patients with colorectal cancer(P＜0.05).Conclusion RXRA and NRIP1 are highly expressed in color-ectal cancer,which are closely related to pathological stage,differentiation degree and metastasis,which can be used as biomarkers to assist in evaluating the prognosis of patients.

Objective? To expLore the knowLedge, attitude and practice status quo of puLmonary rehabiLitation among nurses in respiratory department, and to provide theoreticaL basis for cLinicaL training and quaLity controL. Methods? From JuLy to August 2018, a totaL of 134 respiratory nurses who participated in a continuing education training course in Sichuan Province were convenientLy seLected as the research objects. The questionnaire of knowLedge, attitude and practice of Lung rehabiLitation of nursing staff was used to investigate them. The univariate anaLysis of knowLedge, attitude and practice of Lung rehabiLitation of nursing staff were anaLyzed by t test and variance anaLysis. ResuLts? The totaL score of knowLedge, attitude and practice in puLmonary rehabiLitation among nurses was (101.63±19.58). The average scores of the three dimensions from high to Low were attitude (4.07±0.98), practice (3.08±1.09) and knowLedge (3.03±1.01). There were statisticaL differences in the totaL score of knowLedge, attitude and practice of nurses with different professionaL titLes and whether they had received Lung rehabiLitation training or not (P＜0.05). ConcLusions? The knowLedge, attitude and practice of puLmonary rehabiLitation of nursing staff in respiratory department need to be improved. Nursing administrators shouLd strengthen guidance and training of puLmonary rehabiLitation and strengthen nurses' abiLity to impLement puLmonary rehabiLitation so as to ensure the effective impLementation of puLmonary rehabiLitation in cLinicaL work.

Objective:To explore whether there is Warburg effect in keloid fibroblasts (KFs) , hypertrophic scar fibroblasts (HSFs) and atrophic scar fibroblasts (ASFs).Methods:KFs, HSFs, ASFs and NFs were isolated and cultured, respectively, in vitro. Glucose consumption and lactate production of the cells in each group were tested and compared. The mRNA expression of key glycolytic enzymes was detected, including hexokinase 2 (HK2), fructose-2, 6-biphosphatase 3 (PFKFB3), pyruvate kinase isoform M 2 (PKM2) and lactate dehydrogenase A (LDHA). In addition, the effect of 2-deoxy-D-glucose(2-DG) on the proliferation of KFs and NFs were detected and compared.Results:Glucose consumption and lactate production in KFs were 45.5% and 38.1% higher than those in NFs respectively ( P<0.01), while there was no statistical difference in glucose consumption and lactate production between ASFs and NFs, HSFs and NFs ( P>0.05). The mRNA expression of HK2, PKM2 and LDHA genes in KFs were 4.7, 2.7 and 1.8 times higher than those in NFs ( P<0.05), respectively, while the gene expressions in ASFs and HSFs had no statistical differences from those in NFs ( P>0.05). Under the action of 2-DG, the cell proliferation activity in KF group and NF group were reduced by 37.5% and 27.0%, respectively, indicating that the inhibition effect of glycolysis inhibitor on the proliferation activity of KFs was significantly higher than that of NFs. Conclusions:There is Warburg effect in KFs and there are no similar phenomena in HSFs and ASFs.

Objective To analyze the relationships of the expression of retinoid X receptor alpha(RXRA)and nuclear receptor interaction protein 1(NRIP1)in colorectal cancer tissues with the fea-tures of clinical pathology and prognosis.Methods A total of 106 specimens from colorectal cancer tissues of patients during surgery were included in colorectal cancer group(n=106),and corre-sponding paracancer tissue specimens were included in paracancer group(n=106).The expressions of RXRA and NRIP1 in colorectal cancer tissues were detected by immunohistochemistry.Multivariate Cox regression analysis was used to investigate th4e effects of RXRA and NRIP1 expressions on the prognosis of patients with colorectal cancer.Results The positive expression rates of RXRA and NRIP1 in colorectal cancer group were 66.04％,69.81％,which were significantly higher than 33.96％and 30.19％in the para-cancer group(P＜0.05).The positive expression rates of RXRA and NRIP1 in patients with pathological stage Ⅲ,low differentiation,serous infiltration and lymph node metastasis were higher than those in patients with pathological stage Ⅱ,medium and high differ-entiation,no serous infiltration and lymph node metastasis(P＜0.05).The 3-year overall survival rates of patients with pathological stage Ⅱ,low differentiation,no serous infiltration,no lymph node metastasis,negative RXRA and NRIP1 were higher than those of patients with pathological stage Ⅲ,medium and high differentiation,serous infiltration,lymph node metastasis,positive RXRA and NRIP1(P＜0.05).Multivariate Cox regression analysis showed serous membrane infiltration(HR=2.687;95％CI,1.531 to 3.156),positive RXRA(HR=3.743;95％CI,2.217 to 5.992),positive NRIP1(HR=2.641;95％CI,1.124 to 4.757)were influencing factors for prognosis in patients with colorectal cancer(P＜0.05).Conclusion RXRA and NRIP1 are highly expressed in color-ectal cancer,which are closely related to pathological stage,differentiation degree and metastasis,which can be used as biomarkers to assist in evaluating the prognosis of patients.

Objective:To explore whether there is Warburg effect in keloid fibroblasts (KFs) , hypertrophic scar fibroblasts (HSFs) and atrophic scar fibroblasts (ASFs).Methods:KFs, HSFs, ASFs and NFs were isolated and cultured, respectively, in vitro. Glucose consumption and lactate production of the cells in each group were tested and compared. The mRNA expression of key glycolytic enzymes was detected, including hexokinase 2 (HK2), fructose-2, 6-biphosphatase 3 (PFKFB3), pyruvate kinase isoform M 2 (PKM2) and lactate dehydrogenase A (LDHA). In addition, the effect of 2-deoxy-D-glucose(2-DG) on the proliferation of KFs and NFs were detected and compared.Results:Glucose consumption and lactate production in KFs were 45.5% and 38.1% higher than those in NFs respectively ( P<0.01), while there was no statistical difference in glucose consumption and lactate production between ASFs and NFs, HSFs and NFs ( P>0.05). The mRNA expression of HK2, PKM2 and LDHA genes in KFs were 4.7, 2.7 and 1.8 times higher than those in NFs ( P<0.05), respectively, while the gene expressions in ASFs and HSFs had no statistical differences from those in NFs ( P>0.05). Under the action of 2-DG, the cell proliferation activity in KF group and NF group were reduced by 37.5% and 27.0%, respectively, indicating that the inhibition effect of glycolysis inhibitor on the proliferation activity of KFs was significantly higher than that of NFs. Conclusions:There is Warburg effect in KFs and there are no similar phenomena in HSFs and ASFs.

OBJECTIVETo analyze the polio immunity level of persistent population in Beijing, 2012.

METHODSA total of 1 676 subjects residing more than 6 months in Beijing were selected by stratified random cluster sampling design in 2012. Demographic characteristics, history of oral poliovirus vaccine (OPV) immunization were investigated by questionnaire. All 5 ml blood sample were collected for testing of polio neutralizing antibody using the method of microcell neutralization. The positive rate and the geometric mean titer (GMT) of polio neutralizing antibody type I, II and III were analyzed in different groups.

RESULTSThe positive rate of type I, II and III were 98.2% (1 645/1 676), 98.1% (1 644/1 676), 97.6% (1 635/1 676); The GMT were 1:130.2, 1: 113.4 and 1: 79.7. Three types of positive rates in<15 years group (99.7% (664/666), 99.8% (665/666), 99.5% (663/666)) were higher than those of ≥ 15 years group (97.1% (981/1 010), 96.9% (979/1 010), 96.2% (972/1 010)), the differences were significant (all the values of P < 0.01); The GMT in<15 years group (1:325.9, 1:250.5, 1:190.7) were higher than that of ≥ 15 years group (1: 71.1, 1: 67.2, 1: 44.8), the difference was significant (all the values of P < 0.01). The positive rate (99.0%-100%) and GMT (1: 128.8-1: 300.7) in vaccination information confirmed population were higher. The highest positive rate (all were 100%) and GMT(1: 409.7-1: 636.7) were observed in children who vaccinated three times.

CONCLUSIONThe polio antibody of healthy population was at a high level in Beijing in 2012; Especially the age groups of < 15 years which were covered by vaccines.Immunization barrier had been formed firmly to interrupt the transmission of wild poliovirus and vaccine-derived poliovirus.

Adaptive Immunity ; Adolescent ; Antibodies, Neutralizing ; Antibodies, Viral ; Child ; Humans ; Poliomyelitis ; Poliovirus ; Poliovirus Vaccine, Oral ; Vaccination ; statistics & numerical data