Objective To evaluate mesh plug hernia repair in clinical application.Methods One hundred and eight cases of mesh plug hernia repair and eighty five cases of Bassini repair were retrospectively reviewed in our hospital from Aug. 1998 to Feb. 2000. Results Mesh plug hernia repir patients enjoyed less blood loss, earlier up and about, shorter postoperative febrile period and hospital stay, less postoperative dysuria ratio(all P

Objective To investigate the psychological status of family members of patients with depression, and discuss the health education pathway. Methods The families of 200 inpatients with major depression accepted systemic health education about depression according to the investigation of the self-designed questionnaire. They were assessed with Self-rating Depression Scale (SDS) and Self-rating Anxiety Scale (SAS) when the patients admitted and accepted 8 weeks of treatment. Results The score of SDS was more than 50 in 76.5% families of patients, and it was 79.0% for SAS when admission. The scores of SDS and SAS reduced after education (P<0.01). Conclusion Health education can improve the psychology of family members of patients with depression.

Objective To discuss the drug resistance and the vicissitude of Pseudomonas aerugi-nosa in Intensive Care Unit (ICU), in order to provide basis for rational use of antibiotics in clinic. Methods Identification of bacteria was performed by applying ATB Expression Identification System produced by BioMerieux Company; drug susceptibility tesr was done by using K-B method. The drug resistance of Pseudomonas aeruginosa was compared between ICU and general wards; and then the difference of drug resistance was investigated between 2006 and 2007. Resnlts The more sensitive an-tibiotics to Pseudomonas aeruginosa in ICU of our hospital were as follows, amikacin (resistance rate 6.7%-9.4%), eiprofloxacin (resistance rate 7.7%-24.7%), cefoperazone/sulbactam (resistance rate 11.5%-34%), ceftazidime (resistance rate 11.5%-37.7%), aztreonam (resistance rate 20.2%-40.6%). The drug resistance rate of ICU was higher than that of the general wards, and the drug re-sistance rates to ciprofloxacin, ceftazidime, cefoperazone/sutbaetam,meropenem, imipenem and aztreo-nam in 2007 were significantly higher than those in 2006 (P<0.01). Conclusion The drug resistance rate of Pseudomonas aeruginosa in ICU of our hospital was very high,and it is rising year by year. So it is important to retrospect the results of the drug resistance periodically and provide the up-to-the-mi-nute data of epidemiology and vicissitude of drug resistance.

Objective To evaluate the role of myocardial contrast echocardiography (MCE) to select target vessel, to monitor the opacification zone and to avoid complications during transcoronary ablation of septal hypertrophy(TASH) for hypertrophic obstructive cardiomyopathy(HOCM). Methods In 37 HOCM patients cardiac apex four chamber view, parasternal left ventricular long axis view and left ventricular short axis view were recorded after injection of the myocardial agents and absolute ethanol 5 minutes later respectively. And the opacification zones of the myocardial agents and absolute ethanol were observed. Results Among 37 patients,2 patients gave up treatment because of no appropriate vessel to provide ablation by coronary Doppler flow imaging.Two of 37 patients gave up treatments and did not undergo myocardial contrast echocardiography because of diffused coronary arteries in coronary angiography. Among 33 patients who underwent MCE, 5 patients gave up TASH including one of the opacification of left ventricular mammary muscles, one of the opacification of right ventricular and right ventricular mammary muscles, one for the opacification zone out of the target area and two for the large opacification zone. Other 28 patients underwent TASH successfully. Just after operations and 7 days, left ventricular outflow tract pressure gradient decreased at no less than 50%. Distribution zone of absolute ethanol was smaller than that of myocardial opacification zone in some patients. Conclusions MCE is a credible reference for selection of target vessels in TASH for HOCM.

0. 05 ). For detection of pancreatic duct dilation and lymph node metastasis, EUS was better than CT ( P=0.039,P=0.004). The accuracy of EUS in determing peripancreatic fat infiltration and organic invasion was correlated with tumor size (P=0.015,P=0.022) , pancreatic duct dilation was correlated with the location of tumors ( P

Objective To explore whether Cl2MDP-loaded gelatin particles can induce the depletion of macrophage or can depress the immunity of macrophage in SD rat models of immune thrombocytopenic purpura(ITP) to treat the ITP rats.Methods SD rats were intravenously injected with 150 ?L of anti-platelet serum once per 24 hr to induce the ITP model.The platelet count was persistently less than 50?109L-1 in these models.After intravenous injection of a group dose of Cl2 MDP-gelatin particles,the platelet counts of these models were examined at the time of 4 hr,24 hr,48 hr,72 hr and 96 hr,respectively.The pathological change of macrophages in spleen was observed by electron microscopy.Results Cl2MDP-loaded gelatin particles can significantly increase the platelet counts of ITP models from less than 50?109L-1 to mean 180?109L-1 in 24 hr after injection.The pathological changes of macrophages were seen under electron microscope,such as degeneration, necrosis and so on.Furthermore,rats pre-treated with Cl2 MDP-loaded gelatin particles can evade the decrease of platelet counts when they were injected anti-platelet serum.Conclusion Cl2 MDP-loaded gelatin particles can promptly,effectively restoreplatelet counts of ITP models to physiological level with a dose dependent manner.So,the targeting therapy of drug-loaded gelatin particles offers a new approach to treat ITP.

In this experiment, we introduced human interleukin-2(lL-2)gene and Neomycin resistance (Neo~R) gene into a human lung adenocarcinoma cell line GLC, using a retroviral vector PLXSN. The positive cells obtained by selecting in G418 were compared with the wild-type cells. We observed their growth characteristics in vitro and the quantity of IL-2 in the culture supernatants. PCR result demonstrated the integration of foreign genes into tumor genome DNA after transfection and permanent existence. Under electron microscope, what can be seen was the surface villi of trans-duced-cells are less and shorter. It deserves further investigation to determine whether the change of cell surface influence metastatic ability of cells.

Objective To investigate the mRNA expression of ciliary n eurotrophic factor on the retina during injury and repair of optic nerves in rat s. Methods Thirty-five healthy SD rats were randomly divided into 3 groups: 5 in the control group, 15 in the simply transected optic nerve g roup and 15 in the optic nerve-sciatic nerve anastomosis group. The simply tran sected and optic nerve-sciatic nerve anastomosed models were set up, and the re tinal tissues of all of the rats were taken out after 3, 7 and 14 days, respecti vely; and the mRNA expression of CNTF in the 3 groups were observed by semi-qua ntitative reversal transcription-polymerase chain reaction method. Res ults A minimum expression of CNTF mRNA was found in the reti nae of the control group, and the increased rates of expression were found in th e retinae of the simple transection of optic nerve group with the increase rate of 100%, 594%, and 485% on the 3rd, 7th, and 14th day respectively after the ope ration, while in optic nerve-sciatic nerve anastomosis group, the increase rate s were found to be 258%, 752% and 515% on the 3rd, 7th, and 14th day respectivel y after the operation. Conclusion Retinal neurons can respond to axonal reaction of retinal ganglion cells by up-regulate endogenous CNTF aft er the injury of the optic nerves, which may provide a theoretic base for the application of the exogenous CNTF.

Objective To investigate the effects of Shuxuetong Injection (SXT ) on expressions of cell apoptosis and TLR4 a-round ischemic area after focal cerebral infarction in rats and to discuss its neuroprotective mechanism on ischemia-induced brain in-jury .Methods The SD rats were subjected to establish the model of middle cerebral artery occlusion (MCAO)by nylon monofila-ment suture ,then were randomly divided into the sham-operated group ,the model group and the SXT treatment group ;the cell ap-optosis and the expressions of TLR4 mRNA and protein around ischemic area at 12 ,24 ,48 ,72 h after cerebral ischemia were detec-ted respectively by TUNEL test mediated with DNA ,RT-PCR and immunohistochemistry .Results In the model group ,the number of TUNEL positive cells ,the expressions of TLR4 mRNA and protein were gradually increased at 12 h ,reached the peak at 24 h , then decreased and were still higher than those in the sham-operated group(P<0 .01);in the SXT treatment group ,these expres-sions after 24 h were lower than those in the model group (P<0 .05)and declined as the treatment time increase(P<0 .05) .Conclu-sion In subacute stage of cerebral ischemia injury ,apoptosis is related with the expression of TLR4 ,SXT may inhibit apoptosis , down-regulate the expression of TLR4 around ischemic area ,this may be one of the mechanisms of neuroprotection .

Objective To assess the value of two-dimentional strain/strain rate (2D-S/SR) imaging compared with endomyocardial biopsy (EMB) in detecting acute rejection (AR) reaction after heart transplantation (HT). Methods Twenty-five patients who required HT underwent echocardiography within 12 h after EMB. Ten patients of grade 0 AR were regarded as group A, 8 patients with grades Ⅰa-Ⅰb were considered as group B, 7 patients with grades ≥Ⅱa were considered as group C. Thirty age-matched normal controls were considered as group D. The longitudinal, radial and circumferential stain and strain rate of different ventricular wall in systolic were measured and compared among groups. Results Compared with the group D, longitudinal stain (LS) of basal-septum, circumferential strain rate (CSR) of ant-septum at the level of mitral valve, radial strain (RS) of all segments at the level of mitral valve reduced in groups A, B and C. Conclusion The RS of 2D-S/SR is an effective and sensitive technique for the detection of acute rejection, and longitudinal stain can be used as an early index of AR for grade ≥Ⅱ after HT.