OBJECTIVE: To compare safety and efficacy between anterior cervical decompression and fusion procedures as well as compound titanium rete implantation for the treatment of cervical spondylotic myelopathy using Meta analysis. DATA SOURCE: The relative articles were retrieved from Chinese Biomedical Literature Database (CBM) from January 1998 to December 2008, CNKI from January 1994 to December 2008, and VIP from January 1989 to December 2008. DATA SELECTION: A clinically randomized controlled study was used to evaluate safety and efficacy between anterior cervical decompression and fusion procedures as well as compound titanium rete implantation for the treatment of cervical spondylotic myelopathy. Control or vertebroplasty was excluded. MAIN OUTCOME MEASURES: JOA score, complication, and bone fusion rate. RESULTS: Six clinically control studies were included in the final analysis containing 443 patients. Among the patients, 190 cases were treated with anterior cervical decompression and fusion procedures alone, and 253 with anterior cervical decompression and fusion procedures as well as compound titanium rete implantation. All the 6 controls were performed with Meta analysis. ① Efficacy of anterior cervical decompression and fusion procedures as well as compound titanium rete implantation was superior to that of anterior cervical decompression and fusion procedures [OR=2.30, 95%, CI (1.32, 4.01), P

The effects of endotoxin on protein phosphorylation, calcium transportationand fibrinogen receptor exposure in human platelets were studied. The proteins from ~(32)P-labled human platelets were separated by SDS-polyacrylamide gel electroporesis, then theprotein phosphorylation were observed with autoradiographic method. The result showedthat endotoxin rapidly induced phosphorylation of 20kD protein. An enhanced phosphory-lation of 20kD protein was seen in the presence of extracellular Ca~(2+). Meanwhile, endo-toxin induced an increase of the cytoplasmic free Ca~(2+) concentration and promoted ~(45)Ca_(2+)uptake by platelets. In addition, the platelets to which FITC-labled fibrinogen was boundhad increased. It suggested that the mechanism of endotoxin induced platelet aggregationinvolved in Ca~(2+) mobilization and protein phosphorylation, which caused the fibrinogenreceptor exposure.

Objective To design a hospital broadcast system with infrared remote control using phonetic chip ISD4004.Methods The author used AT89S52 and infrared remote control module to achieve speech recording and playing in hospital.After simply analyzing ISD4004 working-principle,the author designed the software and hardware modules,and realized a digital voice storage and playback.Results The hospital broadcast system was built based on ISD4004 voice chip and infrared remote control.Conclusion The system provides a low-cost and high-performance hospital automatic broadcast solution and realizes intelligent and automation broadcast.

Objective To design a new multilevel distributed community-oriented health monitoring based on two serial ports MCU of W77E58.Methods Multi-machine serial communication with high-performance and full-duplex was realized by using the standard RS422 serial interface,which involved several parts in,such as detection and processing module of physiological parameters,communication module and display processing module of the host computer.With the different functional modules and software and hardware connection,multilevel distributed health monitoring was realized.Results RS422,being a simple,easy to control and low-cost communication mode,implemented the communication between these modules.Finally,the distributed measurement & control system with one PC and several MCU systems was implemented.Conclusion A low-cost,high-performance community-oriented health monitoring solution was realized,which can be easily applied to mobile monitoring at home.

Objective:To investigate the effect of microRNA-146a (miR-146a) on apoptosis of human retinal microvascular endothelial cells (HRMECs) induced by high glucose and its possible molecular mechanism.Methods:HRMECs were cultured in vitro with 5.5 mmol/L D-glucose in the normal control group and 25 mmol/L D-glucose in the high glucose group for 48 hours, respectively.Normally cultured HRMECs were transfected by miR-146a mimics in the high glucose+ miR-146a mimics group or corresponding mimics control in the high glucose+ mimics control group by lipofection and cultured with 25 mmol/L D-glucose for 48 hours, respectively.Real-time fluorescent quantitative polymerase chain reaction (PCR) was performed to detect the expression level of miR-146a.MTT assay and flow cytometry were used to detect the activity and apoptosis of HRMECs, and Western blot was employed to detect the expression levels of apoptosis-associated protein B-cell lymphoma factor-2 (Bcl-2), Bcl-2 related X protein (Bax) and nuclear factor-κB (NF-κB) signaling-related proteins NF-κB p65 and p-NF-κB p65. Results:The relative expression levels of miR-146a were 1.00±0.10, 0.22±0.02, 0.21±0.02 and 0.88±0.09, and the cell viability was (100.00±10.06)%, (68.41±6.67)%, (67.91±6.74)% and (90.46±8.97)%, and the apoptosis rates were (3.11±1.02)%, (27.28±3.56)%, (27.44±4.03)% and (7.29±2.11)% in the normal control group, high glucose group, high glucose+ mimics control group and high glucose+ miR-146a mimics group, respectively.The relative expression levels of miR-146a and the cell viability were significantly lower, and the cell apoptosis rate was significantly higher in the high glucose group than those in the normal control group, with statistical significant differences (all at P<0.05). The relative expression levels of miR-146a and the cell viability were significantly higher, and the cell apoptosis rate was significantly lower in the high glucose+ miR-146a mimics group than those in the high glucose group and the high glucose+ mimics control group, and the differences were statistically significant (all at P<0.05). The relative expression levels of Bax and p-NF-κB p65 protein were significantly higher, the relative expression level of Bcl-2 protein was significantly lower in the high glucose group than those in the normal control group, showing statistically significant differences (all at P<0.05). The relative expression levels of Bax and p-NF-κB p65 protein were significantly lower, and the relative expression level of Bcl-2 protein was significantly higher in the high glucose+ miR-146a mimics group than those in the high glucose group and the high glucose+ mimics control group, and the differences were statistically significant (all at P>0.05). There was no significant difference in the relative expression of NF-κB p65 protein among the groups ( F=0.106, P=0.955). Conclusions:Overexpression of miR-146a may inhibit the apoptosis of HRMECs induced by high glucose, and its mechanism may be related to the inhibition of NF-κB signaling pathway activation.

Objective To evaluate out-patient individual health education prescription by diabetes nurse specialist on related knowledge of type-2 diabetes mellitus and blood glucose.Then combined with patient satisfaction surveys to explore the intervention value of out-patient individual health education prescription.Methods 100 out-patients with type-2 diabetes mellitus were chosen,and gave them individual health education prescription by evaluate individual condition and existing problems with previous treatment protocol unchanged,the prescription content included individual condition,diet,exercise,medication instructions and disease condition monitoring.The grasp of related knowledge of type-2 diabetes mellitus and satisfaction degree with diabetes nurse specialist were investigated by questionnaires.Results 3 months after individual health education,The grasp of related knowledge of type-2 diabetes mellitus,blood glucose and 2 hours' postprandial blood glucose greatly alleviated.Total satisfaction degree of patients reached 94%.Conclusions Paying attention to individualized instruction and out-patient individual health prescription in prevention and treatment of type-2 diabetes mellitus accords with humanization of nursing service,and is a powerful measure to improve health education quality.

The relationship between the hepatic ischemia/reperfusion (I/R) injury and the balance of nitric oxide/endothelins (NO/ET) was studied. The changes of the ratio of NO/ET and the hepatic injury were observed in a rat hepatic I/R model pretreated with several tool drugs. In the acute phase of hepatic I/R injury, the ratio of plasma NO/ET was reduced from 1.58 +/- 0.20 to 0.29 +/- 0.05 (P < 0.01) and the hepatic damage deteriorated. NO donor L-Arg and ET receptor antagonist TAK-044 could alleviate the hepatic I/R injury to some degree, whereas NO synthase inhibitor L-NAME aggravated the damage. It was concluded that the hepatic I/R injury might be related with the disturbance of the NO/ET balance. Regulation of this balance might have an effect on the I/R injury.
Arginine ; Endothelins/*blood ; Liver/*blood supply ; NG-Nitroarginine Methyl Ester ; Nitric Oxide/*blood ; Receptors, Endothelin/antagonists & inhibitors ; Reperfusion Injury/*blood

The effects of estradiol and tamoxifen on the proliferation of estrogen receptor positive cells and the relationship between the tamoxifen tolerance and cell origin were investigated. The tissues of human endometrium and breast cancer were randomly selected following dissection for primary cell culture. After the breast cancer cells and endometrial cells were treated with 1 x 10(-8) mol/L estradiol and/or 1 x 10(-6) tamoxifen, 3H-labelled thymine nucleotide was used to trace the kinetics of cell proliferation. There was no significant difference in the inhibition on the human endometrial cells between tamoxifen-treated group (6.3%) and control group (6.4%), but tamoxifen could significantly inhibit the proliferation of the human breast cancer cells (45.84%) as compared with control group (52.72%). Moreover, tamoxifen could significantly stimulate the proliferation of tamoxifen resistant breast cancer cells (9.64%) as compared with control group (6.32%). Estradiol could significantly stimulate the proliferation of all the three kinds of cells as compare with control group. The combined use of estradiol and tamoxifen could inhibit the proliferation of the endometrial cells and breast cancer cells as compared with estradiol used alone, but on the tamoxifen resistant breast cancer cells, they could more significantly stimulate the proliferation than E2. It was concluded that E2 could stimulate the proliferation of these three kinds of cells. However, the inhibitive effects of tamoxifen on the proliferation of these cells were dependent on the estradiol.
Antineoplastic Agents, Hormonal/*pharmacology ; Breast Neoplasms/*pathology ; Cell Division/drug effects ; Cells, Cultured ; Drug Interactions ; Endometriosis/pathology ; Endometrium/*pathology ; Estradiol/*pharmacology ; Tamoxifen/*pharmacology ; Tumor Cells, Cultured

Objective To study chromatography fingerprints of Zanthoxylum echinocarpum by HPLC.Methods Diamonsil C18 column(250 mm?4.6 mm,5 ?m) was used with acetonitrile-water gradient elution,at flow-rate of 1.0 mL/min,detection wavelength at 278 nm.Results HPLC was used to establish fingerprint chromatograph of water extraction from ten batches of Z.echinocarpum,which had been harvested from the same area in Zhangjiajie at the same time.The result showed that 11 peaks were common.Conclusion The method could be used for the quality control of Z.echinocarpum.