Objective To develop developer with fast determining stopped spot of electrolyte electrode in wartime.Methods According to certain proportion configuration,the developer was prepared by using relative reagent and distilled water.Through injector to take 1ml developer and curve syringe needle,then straight insertion the type needle of electrolysis meter and pour into the developer to fill suction tube.Results The reagent can be seen various electrodes change and fast display the electrode breakdown anywhere.Conclusion The color developing reagent is low-cost,efficient and convenient to use.

The abilities of self-study,thinking and communication are much more emphasized in the problem based learning(PBL)than in the conventional curriculum.From the view of the inherent relationship between the ability training and PBL,this paper probes into the characteristics of PBL to find the way of fitting the situation of our country.It is essential to the successful teaching and training more high quality students.

Objective To investigate the effects of penehyclidine hydrochloride injection combined with safflower injection on TLRs/NF-κB signaling pathway and T cell subsets in mononuclear cells of patients with organophosphorus pesticide poisoning. Methods 80 cases of organophosphate poisoning patients from June 2014 to June 2016 in onglu first people's hospital were selected,randomly divided into control group and observation group, the control group was treated with penehyclidine hydrochloride,observation group were treated with Honghua injection based on the treatment of control group. The effects were compared between two groups of TLRs/NF-κB in peripheral blood mononuclear cells pathway and T cell subsets in peripheral blood, and compared two groups of patients with cholinesterase activity. Results On the third,fifth and 7th day of treatment,cholinesterase activity in both groups was higher than that before treatment,the activity of cholinesterase in the observation group was significantly higher than that in the control group (P<0.05).After treatment,compared with before treatment,the levels of TLR2,TLR4 and NF-κB in the two groups were significantly decreased,and TLR2,TLR4,NF-κB levels in the observation group were lower than those in the control group, the difference was statistically significant (P<0.05),Th17,Th17/Treg levels in the observation group were significantly lower,and lower than the control group,Treg content was significantly higher,and higher than the control group,the difference was statistically significant (P<0.05). Conclusion Penehyclidine hydrochloride injection combined with safflower injection can help to reduce Th17,Th17/Treg levels,increased Treg cell content,regulate the inflammatory response, and promote recovery of cholinesterase activity.

Objective To investigate the effect of ketamine on the phosphodiesterase (PDE) activities in lung tissue and leukocytes in rats. Methods Twenty SD rats weighing 140-160 g were randomly divided into two groups of 10 animals each : (A) ketamine group received intraperitoneal ketamine 10 mgkg-1 and (B) control group received equal volume of normal saline ip. Thirty minutes after ketamine administration the animals were sacrificed by exsanguination. Leukocytes were separated and 25 mg of lung tissue was obtained for determination of PDE activities by HPLC. Results The PDE activities in both lung tissue and leukocytes were lower in ketamine group than those in control group ( P

Objective To observe the level of social support and self-efficacy of the breast cancer patients with postoperative chemotherapy, and study the relationship between them. Methods 76 patients were investigated with Social Support Rating Scale and Strategies Used by Patients to Promote Health (SUPPH). Results The total and every dimension scores of SUPPH and Social Support decreased in the patients. The total and every dimension scores of Social Support positively correlated with every dimension scores of SUPPH (P<0.01). Conclusion The level of self-efficacy and social support may relate each other.

Objective To investigate the effect of standardization regional lymphadenectomy in radical resection of ductal adenocareinoma in the pancreatic head.Methods On the basis of routine panereaticoduodenectomy(whipple proceduce),we performed the standardization regional lymphadenectomy,the emphasis of the procedure was the resection of wide nodes,particularly the mesenteric root lymph nodes(14abcd),the paraaortic lymph nodes(16a2b1),the hepatoduodenal ligament lymph nodes(12abpeh),hepatic artery(Group 8)and coeliacus lymph nodes(Group 9). Results Eleven patients underwent this procedure,there was no operative mortality,1 case occurred pancreatic fistula,the lymph nodes metastasis occurred in 7(63.6%)patients,the posterior pancreaticoduodenal lymph nodes(Groupl3)and superior mensenteric artery(Groupl4)was the predominant metastatic site of the tumor,the rate of the second site metastasis to lymph nodes was 57.1%.of the 11 cases follow-up 1 died. Conclusion The radical pancreaticoduodenectomy combined with wide resection of standardization regional lymph nodes is a safe and effective procedure of the treatment of adenocarcinoma of the head of the pancreas.but the long-term survivalrate must be valued objectively with many cases.

Objective To investigate the effects of captopril on reperfusion arrhythmias in atherosclerotic rabbits.Methods A total of 32 New Zealand white rabbits were randomly divided into the control group,cholesterol food group(CF group) and captopril plus cholesterol food group(Cap group).The levels of endothelin(ET),malonaldehyde(MDA) and the content of nitric oxide(NO) were measured in different stage.The duration of ventricular tachycardia and the incidence of ventricular premature beats in 30 minutes after reperfusion were detected.Results The levels of ET and MDA were significantly decreased and the content of NO were significantly increased in the Cap group compared with that of the CF group.The average duration of ventricular tachycardia and the incidence of ventricular premature beats in 30 minutes after reperfusion were significantly reduced in the Cap group compared with the CF group.Conclusion The incidence of arrhythmias induced by the ischemia reperfusion injury may be decreased with the treatment of captopril in the atherosclerotic rabbits.

[Objective] To observe the protective effect of Yugan Tablet (YT) on D-galactosamme-induced acute hepatic injury in mice. [ Methods ] Mice were randomly allocated to normal control group, model group, Ganpi Kang Capsule group and YT groups (low-, moderate- and high-dosage YT). Serum levels of aspartate transaminase (AST) and albumin (ALB) and clotting time (CT) were examined to evaluate the effects of YT and Ganpi Kang Capsule on mice with D-galactosamine-induced acute hepatic injury. [Results] Serum level of AST was increased ( P

AIM: To investigate the regulatory effects of lipopolysaccharide binding protein(LBP)on activation of p38 signaling pathway induced by lipopolysaccharide(LPS)in alveolar macrophages. METHODS: The LBP from actue phase rat serum was purified by ammonium sulphate precipitation, Bio-Rex70 resin and the MonoQ column. Rat alveolar macrophages were exposed to LPS (0 01 mg/L or 1 mg/L) the various concentrations of LBP(0 mg/L, 0 01 mg/L, 0 1 mg/L,1 mg/L and 10 mg/L) Western blotting were used to detect phospho-p38 in alveolar macrophages RESULTS: SDS-PAGE analysis indicated that the purified preparation of rat LBP showed homogeneity and the molecular weight was 60 kD.The binding of lipopolysaccharide to mononuclear cells were enhanced by purified rat LBP. Stimulation of rat alveolar macrophages with LPS at concentration of 0.01 mg/L was LBP dependent. LBP at concentrations up to 1 mg/L was able to increase the activation of p38. However , when LBP concentrations were further increased to 10 mg/L, the phosphorylation levers of p38 were lower as compared with that in the presence of 1 mg/L. Stimulation of rat alveolar macrophages with LPS at concentrations of 1 mg/L was LBP-independent. CONCLUSION: The activation of p38 induced by LPS at lower concentration(0.01 mg/L ) was LBP-dependent, meanwhile, LPS at higher concentration (1 mg/L ) was LBP-independent.

Objective In order to understand whether recombinant human growth hormone(r-hGH) can improve the idiopathic short stature(ISS) boys' final adult height(FAH).Methods We measured 16 cases diagnosed as ISS boys FAH, divided into 2 groups, of which 9 cases were not given r-hGH treatment, treatment group ,7 patients received r-hGH treatment, a dose of 0.1 ～ 0.13 U· kg-1·d-1, before going to sleep subcutaneous injection, duration of treatment was 6 ～ 20 months ( 11.9 ± 5.2 months).Results The treatment group compared with the average FAH FAH observation group had significantly improved the average(t =2.219,P<0.05).Conclusion r-hGH can improve the ISS boys FAH, but early treatment, the longer the course the more obvious effects.