Enzyme linked lectin assay (ELLA) and lectin cytochemical staining are employed to study of WGA receptor on human sperm membrane of 44 fertile and 40 unexplained infertile males in this experiment. Binding capacity of sperm from fertile and infertile males, on the average, showed 16.3?10~7 and 9.39?10~7 WGA-HRP molecules per sperm, respectively (P

A Review Angiogenesis plays a key role in progression of prostate cancer. Antigiogenesis becomes a new treament target for prastate cancer. In this review, we focus on the current knowledge of angiogenesis and tumor angiogenesis inhibitor in prastate cancer. [

Thymus, lymph node and intestine (two pieces each) were taken from 20 adult guinea pigs and 10 adult rats. One piece was frozen in liquid nitrogen (-70℃) and cryostat sections were stained with Janszen and Nugteren (1971) method to demonstrate prostaglandin synthetase activity, Another piece was fixed in methanol, and paraffin sections were stained with HE and toluidin blue to demonstrate mast cell. Teased preparation of mesentery were stained by the same methods to show enzyme activity. Indomethacin inhibiting test was used as control. It is proven that the brown deposit represented the prostaglandin synthetase activity. These deposit existed in cytoplasm of mast cell, while and was absent in nucleus and specific granules. Prostaglandin synthetase positive mast cells distributed widely in lamina propria, axis of villi and submucosal layer in intestine, capsule, septa and medulla in thymus; capsule, trabecullum and paracortical zone of lymph node.

NM2 3genewasconsideredasatumormetastasissuppressor,itisalsoinvolvedintumor pathogenesis ,therearemoredetailedstudiesinbreastcarcinoma .Thisreviewfocusesontherelationshipbe tweenNM2 3geneandthemetastasisinbreastcarcinoma .

AIM: To explore the cellular mechanisms by which GLP-1(7-36)NH 2 stimulates insulin secretion in ?-cells. METHODS: Using adherent cell analysis and sorting cytometer (ACAS) by DiBAC 4(3) as a probe,effects of glucagon-like peptide-1(7-36)NH 2 on membrane potential was investigated in cultured neonatal rat pancreatic islet ?-cells,and radio immunological methods were used to determine the amount of insulin secretion in different glucose concentrations. RESULTS: In the presence of 2.8 mmol/L glucose,GLP-1(7-36)NH 2 could not increase insulin secretion and could not change membrane potentials of ?-cells;In the presence of 5.6,16.7 mmol/L glucose,GLP-1(7-36)NH 2 produced a marked increase in insulin secretion and enhanced the depolarization of ?-cells.In 16.7 mmol/L glucose, EGTA or nifedipine completely blocked GLP-1(7-36)NH 2-induced insulin secretion and changes in membrane potentials of ?-cells. CONCLUSION: GLP-1(7-36)NH 2 stimulates insulin secretion in a glucose concentration-dependent manner,it can enhance the depolarization of ?-cells caused by glucose.

(man and ram)The interstitial tissue was assessed by both parameters:(A″—A′)/N:ratio of interstitial tissue area and the number of seminiferous tubules;(A″—A′)/A′:ratio of interstitial tissue area and the total cellular area of seminiferous tubules.There is also a siginificant difference between various species for these parameters: the value of guinea pig is the smallest, while the value of swine is the largest.It was proved that the value of (A″—A′)/N of every species is closely related to the value of (A″—A′)/A′, and eight linear regressional equations were established. All these results have provided important data for further study of male reproductive physiology and male contraception research.

Objective To evaluate the effect of shielding at the outside of radiation field by measuring the dose absorbed by the tissue outside the field in radiation therapy.Methods The dose absorbed by the tissue at 1cm offset the field was measured ofter setting radiation field and dose.The dose was measured again with shielding at the outside of radiation field in same field size and with the same prescription dose for comparison.Results The doses with or without shielding had distinct differences(P

Nucleoside analogues have been the mainstay of clinical treatment of herpes simplex virus 1 (HSV-1) infections since their development. However, the emergence of drug resistant strains has underlined the urgency of the discovery of novel anti-HSV-1 drugs. Natural products, which provided many novel drug leads, are known to be an important source of anti-HSV-1 agents. Herein, we present an overview of natural products with anti-HSV-1 activities isolated from a variety of plants reported in recent years. Several different compounds, mainly belonging to the three groups of polysaccharides, polyphenols and terpenes, showed antiviral effects against HSV-1, indicating their potential to be promising anti-HSV-1 agents.

BACKGROUND:Polyhydroxyethyl methacrylate has excel ent optical properties and good hydrophilicity which has been widely applied in biological materials, such as contact lenses, intraocular lenses. In previous
experiments, artificial corneas made of polyhydroxyethyl methacrylate subcutaneously implanted or implanted into normal rabbit cornea have showed good biocompatibility and tear resistance.
OBJECTIVE:To evaluate the histopathological results of the porous skirt of new type one-piece keratoprosthesis made of polyhydroxyethyl methacrylate implanted to alkali burned rabbit corneas.
METHODS:New blood vessels and wal eyes formed in New Zealand rabbits at 3 months after alkali burned rabbit corneas. The porous discs of polyhydroxyethyl methacrylate were inserted into the lamel ar pocket of
alkali-burned corneas and the corneas were observed clinical y, histological y and ultrastructural y at 2, 8, 16 and 28 weeks after implantation,.
RESULTS AND CONCLUSION: Histopathology suggested that mild inflammatory reaction and no calcification were seen in al specimens, fibroblasts and deposition of col agens were found in the pores of the dics at 2 weeks after implantation;stable connection with cornea was formed by the end of 16 weeks;the pores were almost completely fil ed with new tissue, the number of cel s decreased, and mature fibers were mainly found at 28 weeks. Scanning electron microscope showed new tissue grew into the pores which were closely connected with the corneas. Transmission electron microscope exhibited cytoplasm migrating into the material was rich in rough endoplasmic reticula, showing strong synthetic function, col agen, proteoglycans, and other extracel ular matrix deposition. These findings indicate that the porous skirt of polyhydroxyethyl methacrylate implanted into the alkali burned rabbit corneas al owed corneal cel s migration, proliferation, secretion of the deposition of extracel ular matrix and the formation of new tissue to complete the stable connection with cornea, showing a better biocompatibility.

Objective To investigate the clinical efficacy of total parathyroidectomy and autologous transplantation(PTX+ AT) for treating secondary hyperparathyroidism(SHPT) in the patients with uremic maintenance hemodialysis and postoperative calcium supplementation.Methods The clinical data in 57 patients with SHPT treated with PTX+ AT in the urology department of the First Affiliated Hospital of Nanjing Medical University from March 2015 to August 2015 were retrospectively analyzed.The changes of serum intact parathyroid hormone(iPTH),calcium and clinical symptoms were observed before and after operation.Results Serum iPTH levels at different time points of immediately after operation,postoperative 20 min,2 d,4 d were(288.57 ±194.71),(185.00±99.28),(83.83 ± 58.26),(17.91± 15.60) pg/mL respectively,which were significantly decreased compared with (2395.80 ± 829.04) pg/mL before operation (P＜ 0.05);blood calcium at postoperative 4,8,24,72 h was (2.34 ± 0.32),(2.16±0.37),(2.16±0.27),(2.25±0.25) mmol/L respectively,which were significantly decreased compared with(2.61±0.25)mmol/L before operation(P＜0.05);the symptoms such as bone pain,skin itching and so on were significantly improved.Conclusion PTX+AT can effectively treat uremic SHPT,and postoperative timely calcium supplementation can reduce the occurrence of hypocalcemia.