AIM:To establish the quality standard of the composition of Yuquan Micropill(Radix Rehmanniae,Fructus Schisandrae Chinensis,Radix Puerariae lobatae,etc.). METHODS: The composition of Yuquan Micropill were identified by TLC and the content was determined by HPLC.Puerarin could be separated through Diamonsil~(TM) C_(18) column(5 ?m,250 mm?4.6 mm) with methanol-water(1.2∶3 by volume) as a mobile phase.The flow rate was 1.0 mL/min,the detection wavelength was set at 250 nm and the column temperature was maintained at 40 ℃.?-Schizandrin could be separated through Kromasil KR100-3.5 C_(18) column(100 mm?4.6 mm) with methanol-water(3.5∶1 by volume) as a mobile phase.The flow rate was 0.6 mL/min and the detection wavelength was set at 254 nm. RESULTS: The spots in the TLC were clear and could be well separated,the blank and adjuvant contrast could not be interfered.HPLC showed a good peak form,good resolution and could not be interfered by the foreign matter.The average recovery of puerarin was 99.24%,RSD=0.84%,RSD of precision was 0.95%,RSD of reproducibility was 0.86%,RSD of stability was 0.44%.The average recovery of ?-schizandrin was 99.74%,RSD=0.78%,RSD of precision was 1.89%,RSD of reproducibility was 1.76%,RSD of stability was 1.82%. CONCLUSION: The method is simple and has good resolution,strong specificity and good reproducibility,It can be used for the quality control of the composition of Yuquan Micropill.

Objective:To establish a method for analysis of residual organic solvents (methanol, ethanol, acetone, ethyl acetate) in Ginkgo biloba Leaves for injection.Methods: The contents of residual solvents in Ginkgo biloba Leaves for injection were determined by headspace GC on HP 5 column, with FID detector, high purity nitrogen as the carries gas.Results: There was a good linearity ( r :0.9909～0.9999). The RSD of precision and accuracy was less than 5%, the average recovery rate was in the range of 93.06%～113.78%.Conclusions: The method was simple, quick and accurate and can be used for quality control of Chinese patent medicine.

Objective To study the antitumor fraction from liquid submerged fermentation of Ganoderma sinense. Methods The antitumor active fractions were extracted from the hyphostroma which was produced by liquid submerged fermentation and their antitumor activities were observed by mice transplant tumor model. The total polysaccharides were tested by phenol-sulphuric acid method and the contents of reducing sugars were measured by DNS method. The cytotoxic activities were tested by MTT method in vitro. Results The optimum fraction was the intracellular soluble precipitation (sample B) by water extracting with alcohol sedimentation. The contents of total polysaccharide and reducing sugar in the optimum active fraction from liquid submerged fermentation of G. sinense were 88.4% and 3.15%, respectively, but no protein. The inhibitory ratios were 63.94% and 58.32% on the growth of H22 and Lewis tumor transplanted in mice by ig administration in the concentration of 40.5 mg/kg, respectively. The IC50 on four tumor cells of A549, LoVo, CEM, and QGY-7703 were 160, 29.28, 45.06, and 37.38 ?g/mL, respectively, which suggested that the active fraction had weak cytotoxic on LoVo, CEM, and QGY-7703. Conclusion The active fraction from liquid submerged fermentation of G. sinense is polysaccharide which has better inhibitory rate on the growth of H22 and Lewis tumor transplanted in mice but their cytotoxicities are weak.