OBJECTIVE: To investigate the preventive effect of Antine Capsule on the pain(EIP) in patients receiving root canal therapy.METHODS: The medication group(n=60) were assigned to receive Antine Capsule for 3 days after root canal preparation,while the Control group(n=69) to receive routine therapy.The incidence and degree of time-dependent EIP within 7 days after operation were observed.RESULTS: The EIP incidence rate and the degree of pain in the medication group were significant lower than in routine RCT group(P

Objective Through To analyzing analyze the pathogen distribution and resistance analysis of elderly patients with pulmonary infection,in order to provide basis for clinical rational administration.Methods The clinical data of 285 elderly patients diagnosed as pulmonary infection were selected,and their sputum specimens cultivation were collected for statistical analysis,and the pathogenic bacteria distribution and drug resistance were analyzed.Results 292 strains of pathogenic bacteria were detected from 285 patients,of which 204 strains of gramnegative bacteria,accounting for 69.8％,mainly including pseudomonas aeruginosa,acinetobacter baumannii and klebsiella pneumoniae,while 84 strains of gram-positive bacteria,accounting for 28.8％,mainly including staphylococcus aureus and 4 strains of fungus,accounting for 1.4％.Different pathogens showed different degrees of antimicrobial resistance:pseudomonas aeruginosa,acinetobacter baumannii,klebsiella pneumoniae and e.coli had highly sensitivity for imipenem and meropenem,which were secondly sensitive to amikacin and highly resistant to ampicillin;Staphylococcus aureus and epidermis staphylococcus were all sensitive to linezolid and vancomycin,which were highly resistant to benzyl penicillin.Conclusion Gram-negative bacilli is the main bacteria for patients with pulmonary infection,which has higher antimicrobial drug resistant rate,and antimicrobial agents should be rationally used according to drug susceptibility results.

Objective To explore the effect and method of the theory teaching of general practice course for eight-year program of clinical medical students. Methods The course of General Medicine was set up for 87 students from two grades in their fourth school year. All of them attended a closed-book exami-nation when they finished the lectures. The satisfaction rates, necessity and the suggestion of teaching and assessment were collected from the questionnaire. Results The examination results of the two grade students were (80.92±7.26) and (83.76±5.75); The satisfaction rate was 40.2％ (35/87); Necessity of the courses was between 3.6 to 4.1 (5-point-Likert-scale). The way of teaching method students chose was dif-ferent by course content. Conclusion The theory teaching of general practice course for eight-year program of clinical medical students needs more reform from curriculum designing, teaching methods, faculty train-ing and evaluation methods.

Objective： To study the application of DSA in the diagnosis and treatment of ENT diseases. Methods： The diagnostic and therapeutic roles of DSA in ENT patients admitted from November 1995 to December 1999 were retrospectively studied. Results： Therapeutic vascular embolization using DSA was performed in 9/10 patients with severe epistaxis. The treatment was successful in 8/9 patients with a successful rate of 88.89%; embolization of tumor supplying vessels using DSA as a preoperative measure for reducing operative blood loss in 3 patients with nasopharyngeal fibrohemangioma obtained a total success; diagnosis was clarified in 2 patients using DSA. No patients were with severe complications. Conclusion： DSA is not only a safe and effective measure for diagnosis and therapy, but also effective in differential diagnosis of space occupying lesions. Preoperative selective embolization of tumor supplying arteries can reduce operative blood loss.

Objective To investigate the efficacy of entecavir after partial embolization in patients with hepatitis B cirrhosis complicated with hypersplenism.Methods From February 2016 to October 2016,the clinical data of 92 patients with hepatitis B cirrhosis and hypersplenism in the First People 's Hospital of Taizhou were retrospectively analyzed.Among them,46 cases were treated with lamivudine antiviral therapy after partial embolization as the control group.46 cases were treated with entecavir tablets after partial embolization as the observation group.After 36 weeks of contact therapy,liver fibrosis was compared between the two groups:procollagen Ⅲ (PC-Ⅲ),serum hyaluronic acid (HA),collagen Ⅳ (Ⅳ-C).The liver function of the two groups were compared:total bilirubin (TBil),alanine transaminase (ALT),albumin (ALB),aspartate aminotransferase (AST).The the control of hepatitis B virus were compared between the two groups:HBeAg/anti-HBe negative,negative HBV DNA.Results Liver fibrosis:the levels of PC-Ⅲ,HA and Ⅳ-C in the observation group were (93.6 ± 31.3) U/L,(83.2 ± 25.4) U/L and (85.5 ± 25.4) μ mol/L,respectively,which were significantly lower than those in the control group [(148.6 ± 24.4) U/L,(152.2 ± 34.5) U/L and (146.1 ± 36.6) μmol/L],the differences were statistically significant (t =3.457,3.848,4.065,all P ＜ 0.05).Liver function:the levels of TBil,AST and ALT in the observation group were (21.4 ± 5.4) μmol/L,(60.1 ± 20.6) U/L,(52.4 ± 15.4) U/L,respectively,which were significantly lower than those in the control group [(51.1 ± 6.7) μmol/L、(116.4 ± 25.5) U/L、(110.9 ± 20.5) U/L],the differences were statistically significant(t =4.106,3.763,2.945,all P ＜ 0.05).The ALB in the observation group was significandy higher than that in the control group [(31.2 ± 1.5) g/L vs.(35.9 ± 2.8) g/L,t =2.966,P ＜0.05].Hepatitis B virus control:the observation group and control group had 13 cases and 2 cases in HBeAg/antiHBe negative,respectively,the difference was statistically significant (x2 =5.035,P ＜ 0.05);the observation group and control group were 15 cases,2 cases in HBV DNA negative,respectively,the difference was statistically significant (x2 =5.364,P ＜ 0.05).Conclusion The treatment of entecavir by partial embolization of patients with hepatitis B cirrhosis complicated with hypersplenism can well control the liver function and hepatitis B virus replication and reverse the control of liver fibrosis.Antiviral therapy after embolization has an important role.

Objective To investigate the clinical effect and hearing loss of endolymphatic sac decompression(ESD)combined with one or two semicircular canal obstruction(SCO)in treating the patient with stage Ⅲ or stageⅣ Meniere disease.Methods Forty-three patients with stage Ⅲ or stage Ⅳ Meniere disease,who failed to respond to conventional conservative treatment and had the expectation of preserving residual hearing function,were enrolled in the study.They were divided into three groups according to the operation they underwent:ESD combined with lateral and posterior semicircular canal obstruction(ESD+LPSCO)13 cases,ESD combined with lateral semicircu-lar canal obstruction(ESD+LPSCO)14 cases,and ESD only 16 cases.Data of vertigo,ear fullness and tinnitus be-fore and after operation was collected,analyzed and compared.Hearing function before and after the operation was also evaluated.Results The number of vertigo attacks in the three groups(ESD+LPSCO,ESD+LSCO,and ESD)were all significantly reduced after operation.The vertigo control rate were 92.3％,78.6％and 62.5％re-spectively.Compared with pre-operation,the vertigo severity post-operation in the three groups were also signifi-cantly reduced.And the improvement rate of vertigo severity after ESD+LPSCO and ESD+LSCO were both 100％,which were significantly higher than that of ESD(68.8％).The discomfort of tinnitus and ear fullness in the three groups were significantly improved compared with that of pre-operation.The improvement rates of tinnitus in the three groups were 46.2％,50.0％and 43.8％respectively,with no significant difference.The improvement rate of ear fullness in the three groups were 61.5％,57.1％and 50.0％respectively,with no significant difference either.The proportion of patients with decreased hearing after operation in the three groups was 15.4％,7.1％and 18.8％,respectively,and the differences were insignificant.Conclusion ESD combined with one or two SCO can effectively control vertigo and other symptoms of patients with stage Ⅲ or Ⅳ Meniere's disease who had failure to conventional conservative treatment,and can preserve the residual hearing function in considerable extent.

The expression of dentin sialophosphoprotein (DSPP) is the marker for cells differentiated into odontoblasts. This study attempted to analyze the DSPP promoter and build the reporter LacZ expression system driven by this promoter, which allows convenient and quick detection of odontoblast cells. First, we separated the human dental mesenchymal cells in which the expression of DSPP can be effectively induced by dexamethasone. Second, four 5' flanking regions of human DSPP gene (- 4 000-+54, -2 500-+54, -1 447-+54 and -1 027-+54) were analyzed, the results showed that the highest promoter activity lied in the -2 500-+54 region. The promoter activity is reduced when the 5' flanking region was extended from -2 500 to -4 000 bp upstream from the transcription start site; The promoter activity are also decreased when the 5' flanking regions were shorted from -2 500 to -1 447 bp and from -1 447 to -1 027 bp, indicating that potential suppresser elements are lied in the region between -4 000 and -2 500 bp and potential activator elements are lied in the region between -2 500 and -1 027 bp. Then we constructed the lentiviral report vector phDSPP-LacZ containing the -2 500-+ 54 promoter region in front of the LacZ gene. The expression of LacZ was detected using X-Gal staining in both human dental mesenchymal cells and immortalized human dental mesenchymal cells infected with phDSPP-LacZ. The phDSPP-LacZ lentiviral vector may provide a more convenient method to detect the expression of DSPP in human odontogenic differentiation, tooth development and tooth regeneration studies.
Cell Differentiation ; Extracellular Matrix Proteins ; genetics ; Genes, Reporter ; Humans ; Lac Operon ; Odontoblasts ; cytology ; Phosphoproteins ; genetics ; Promoter Regions, Genetic ; Sialoglycoproteins ; genetics