Aim To establish a method for recording tension changes in small artery in vitro.Methods The vessel segments of intestine were dissected from the mesenteric artery of rats and mounted onto Myograph.The internal circumferences of the segments were normalized with Chart5 software.Then the internal diameters of the segments and initial setting of tension were calculated with self-made Excel file.Following these preparation steps, the tension changes induced by several stimulations were recorded by Myograph.Results Normalization procedure provided the data of lumen diameters of small arteries,which was convenient for initial setting of tension and guaranteed the uniformity of the experimental conditions.Myograph could record the tension changes of small arteries induced by several classic stimulations.Conclusion Precise recording method for tension changes in small arteries in vitro is successfully established.

Objective To investigate the correlation of clinical and pathological characteristics of bladder transitional cell carcinoma(BTCC) with platelet-derived endothelial cell growth factor (PD-ECGF). Methods Expression of PD-ECGF,VEGF and MVD were examined in the specimens of 52 cases of BTCC by Envision immunohistochemical staining.All the 52 cases were followed up via interview for a period of 5 years. Results Of the 52 cases 50 provided complete relevant data.The 5-year postoperative tumor-free survival rate was 42.9%(21/49) and overall survival rate was 78.0%(39/50).The positive expression rate of PD-ECGF in G 1 tumors was 17.6%(3/17);in G 2,59.3%(16/27) and in G 3,87.5%(7/8)(P

Objective: To investigate the effect of demethylase obesity-associated protein(FTO) on the expression of tumor necrosis factor-α-stimulating gene-6(TSG-6) mRNA demethylation in keloid tissue and its role in scar formation. Methods: Fresh keloid tissue(experimental group) and normal skin tissue(control group) were randomlyselected, and the expression level of TSG-6 in the experimental group and control group was detected by immunofluorescence method. A probe expressing TSG-6 mRNA sense and antisense chain were constructed for RNA pulldown, followed by Western blot detection of samples in each group with N6-methyladenine(m6A) antibody. Real-time fluorescence quantitative polynucleotide chain reaction(qPCR) and Western blot were used to detect the expression of TSG-6 mRNA demethylase and methylase in the experimental group and control group. The expression levels of FTO in experimental group and control group were detected by immunofluorescence assay. Results: Immunofluorescence results showed that the expression level of TSG-6 in the experimental group was lower than that in the control group(P<0.01). Pulldown test results of biotin-labeled TSG-6 RNA showed that the m6A expression level of TSG-6 mRNA in the experimental group was lower than that in the control group(P<0.01). The results of qPCR showed that the expression level of FTO mRNA in the experimental group was higher than that in the control group(P<0.01). Western blot analysis showed that FTO protein expression in the experimental group was higher than that in the control group(P<0.001). Immunofluorescence results showed that the expression level of FTO in experimental group was higher than that in control group(P<0.001). Conclusion The demethylase FTO catalyzes the demethylation of TSG-6 mRNA and promotes the formation of keloid, which may provide a new idea and direction for clinical treatment of keloid.

Objective: To investigate the effect of secreted protien acidic and rich in cysteine(SPARC) gene on the proliferation and apoptosis of human keloid fibroblasts and its mechanismin vitro. Methods: The lentiviral vector carrying SPARC shRNA(LV2 N-shRNA-SPARC,sh-SPARC group)and empty plasmid(LV2-NC,sh-NC group)were transfected into human keloid fibroblasts(HKF) respectively. Real-time PCR(qRT-PCR) and Western blot were performed to examine the expression of SPARC mRNA and protein in HKF. MTT assay was used to estimate the cell proliferation. The cell cycle and apoptosis of HKF were detected by flow cytometry. The expression of TGF-β1 and TβRⅠ proteins in HKF were detected by Western blot. Results: (1) After transfection of SPARC shRNA lentiviral vector, the expression of SPARC mRNA and protein in HKF were significantly down-regulated compared with those in the control groups(P<0.05).(2) MTT assay showed that the proliferation of sh-SPARC groups decreased compared with the control groups(P<0.05).(3) Flow cytometry results showed that the S phase cell ratios of sh-SPARC groups were reduced while the apoptosis rates increased compared with the control groups(P<0.05).(4) Western blot showed that the protein expression levels of TGF-β1 and TβRⅠsignificantly decreased in shSPARC groups. Conclusion SPARC gene silencing inhibits the proliferation blocks cell cycle progression and promotes cell apoptosis in HKF, which may be related to the down-regulation of the TGF-β signaling pathway.

【Objective】 To evaluate and analyze the impact of COVID-19 epidemic on inventory of red blood cells (RBCs)in local and municipal blood stations in China, and to provide reference for the management of public health emergencies. 【Methods】 Relevant data from 2018 to 2021 were collected, and the differences in the volume of qualified RBCs, the usage efficiency of inventory RBCs, the average daily distribution of RBCs,the blood distribution rate of RBCs prepared by 400 mL whole blood, the difference in the average storage days of RBCs at the time of distribution, the average daily inventory of RBCs and the time of the average daily inventory of RBCs to maintain the distribution in 24 local and municipal blood stations in China during the COVID-19 epidemic and non-epidemic periods were retrospectively analyzed. 【Results】 Compared with non-epidemic periods, the volume of qualified RBCs [(117 525.979 ±52 203.175)U] and the average daily distribution of RBCs [( 156. 468 ± 70. 186) U ] increased significantly, but the usage efficiency of inventory RBCs decreased(97.24%±0.51%) significantly (P<0.05).There was no significant difference in the blood distribution rate of RBCs prepared by 400 mL whole blood(73.88%±20.30%), the average storage days of RBCs distribution(13.040 ±3.486), the average daily stock quantity of RBCs[(2 280.542 ±1 446.538) U ] and the time of the average daily inventory of RBCs to maintain the distribution[(15.062 ±7.453) d] (P>0.5). 【Conclusion】 During the COVID-19 epidemic, the inventory management of RBCs operated well, the overall inventory remained relatively stable, the stock composition and storage period showed no significant change.