Objective:To investigate the protective role of Shenfu injection(SF) in ischemia-reperfusion injury of transplanted livers in rats.Methods:48 male SD rats,weighted 200~230g,were selected and divided into control group and SF group at random.All of them were performed liver transplantation.2h,4h and 6h after the transplantation,they were analyzed for level of biliation,serum alanine aminotransferase,tumor necrosis factor-?(TNF-?),NF-?B expression in hepatic tissue and the morphological observation of hepatic tissue under light/electron microscope.Results:At each phase,biliation in SF group was significantly higher than in control group(P

By dynamical collecting respirator's gas press signal on line and drawing gas press signal wave,this system can measure respirator's press values and analyze press's stability. The system can be used as a teaching demonstration system to display respirator's working status for students.

Objective To investigate the inhibitory effects of hepatitis B virus (HBV) S gene-specific antisense locked nucleic acid (LNA) on HBV replication and expression in HepG_22.2.15 cells,and to screen the effective short sequence of LNA.Methods Four different lengths of short sequence of antisense locked nucleic acid which were complementary to the initiator of HBV S gene were designed,synthesized and transfected by cationic liposomes into HepG_22.2.15 cells.The HBsAg and HBV DNA of supematant was tested by enzyme linked immunoadsorbent assay(ELISA) and real-time fluorescent quantitative PCR(FQ-PCR) at 24,48 and 72 hours after treatment.LNA's cyto-toxicity on cell was evaluated by MTT method.Results Four different lengths of short sequence of LNA inhibi-ted the expression of HBsAg and the replication of HBV DNA with the inhibition rates of 46.58%,54.38%,72.43% ,69.92% and 27.09% ,28.77% ,34.71% ,32.68% respectively after 72 hours.There's no obvious tox-icity on cell.Conclusion Antisense LNA that targeting at HBV S gene has strong inhibition on HBV in vitro,and the optimal length of LNA sequence might be in the range of 15 base to 25 base.It has a therapeutic potential in the treatment of patients infected with HBV.

Objective To develop a mouse model of cutaneous protothecosis.Methods Totally,48 BALB/c mice were randomly and equally divided into four groups:high-and low-concentration immunosuppressive groups-immunosuppressed mice inoculated with P.zopfii var.portoricensis suspension of 1 × 109 and 1 × 106 colony forming units (CFU) conidia/ml respectively,high-concentration healthy group-healthy mice inoculated with P.zopfii var.portoricensis suspension of 1 × 109 CFU conidia/ml,and control group-healthy mice inoculatedwith sodium chloride physiological solution.The P.zopfii suspension or sodium chloride physiological solution was subcutaneously inoculated to the abdominal skin of mice,with the inoculation volume being 200 μl.Skin appearance at the inoculation site was observed,and four mice were sacrificed in each group on day 7,14 and 28 after the inoculation.Skin specimens were resected from the inoculation sites of mice and subjected to pathological and mycological examinations.Results Mice in the three experiment groups inoculated with the P.zopfi suspension were all infected,with the appearance of papules and abscess at the inoculation sites of all mice as well as ulcer and crusts in some mice.Meanwhile,no mice were infected in the control group.Significant differences were noted in the diameter of skin lesions between the three time points in these experiment groups (all P ＜ 0.05),and the largest diameter of lesions was observed on day 7,which was (6.75 ± 1.09) mm in the high-concentration immunosuppressive group,(5.88 ± 1.17) mm in the low-concentration immunosuppressive group,and (5.96 ± 0.99) mm in the high-concentration healthy group.Comparisons of lesion diameter between the three experiment groups revealed a statistical difference on day 28 (F =8.91,P ＜ 0.05),with the largest lesion diameter observed in the high-concentration immunosuppressive group (4.38 ± 0.86 mm),but no statistical difference was found on day 7 or 14 (both P ＞ 0.05).Pathology of skin specimens consistently revealed necrosis,abscess and granuloma formation in the three experiment groups.Spores were found in the lesions of infected mice by haematoxylin-eosin staining,periodic acid-Schiff staining,and direct microscopy.Culture of tissue samples from the experiment groups grew Prototheca.Conclusion The mouse model of protothecosis can be established by subcutaneous inoculation of Prototheca suspension in the abdominal skin of healthy or immunosuppressed mice.

In order to study life science,the cell culture device is the foundation contributing to the research.With the development of technology in the area,cell culture devices have been significantly improved,and different kinds of devices were designed and novel devices also have been invented.This article provides an overview of the history and present situation of the cell culture devices.The future trend is prospected.

Royal jelly (2.5g/kg/d igX8d) increased the clearance rate of iv charcoal particles in normal mice, and completely antagonized the decrease of clearance rate caused by cortisone acetate. It also potentiated the delayed type hypersensitivity ( DTH ) reaction of footpad induced by SRBC in normal mice, and entirely antagonized the inhibition of DTH reaction caused by eye lophosphamide. Royal jelly (2.5 g/kg/d ig X 7 d ) had no marked influence on hemolysin formation in normal mice and immunosuppressed mice caused by eye lophosphamide.

In order to explore the mechanism of cardiac damage by PTH, the release of PGI2 from heart cells was measured. The results showed that bPTH ( 1-34) increased the release of 6-keto-PGFla from heart cells in a dose dependent manner. Calcium inophone A23187 also increased the 6-keto-PGF1? release, while EDTA and verapamil reduced it. These suggest that the PGI2 synthesis in heart cells was affected by intra and extracellular calcium. The significance of bPTH( 1-34) induced increase of PGI2 synthesis might be associated with interference of energy metabolism, and then, cell damage.

This paper reported the estimation of free calcium concentration in rabbit resting lymphocytes, and the effect of (-) Daurisoline (8701) on the cyto-plasmic free calcium concentration by using fluorescent indicator Fura -2/Am estimating technique. Results were compared with the effect of the Ca2-ionophore A23187.The results indicated that the free calcium concentration of resting lymphocytes was 109. 6nmol ? L-1, after 10-5 mol? L-1,10-4.5 mol ? L-1,and 10-4 mol ? L-1-)Daurisoline administration, the cyto-plasmic free calcium concentration increased to 178. 8%, 204. 4%and 817. 5% respectively, while same concentration A23187 only led the increase of 164. 6% , 198.4% and 327% respectively.Results suggested that ( - )Daurisoline may be a Ca2- agonist and may induce the cytotoxicity effect on lymphocytes at high concentr ation.

Objective To investigate the protective effect of exercise on osteopenia female rats caused by ovariectomy and its mechanism. Methods Twenty-four female rats aged 6 months were randomly divided into 3 groups: control group(CON),ovariectomized model group(OVX) and exercise group(Ex).The ovariectomy operation was performed with rats in OVX and Ex groups. Rats in Ex group were trained on a treadmill at a velocity of 18m/min for 1h, once daily for 5 days a week. Each group of rats were sacrificed at 12 weeks postoperatively and Dual-energy X-ray absorptiometry was carried out to measure the bone mineral density(BMD) of the femur. IGF-1 proteins in trabecular bone of proximal femur were examined by using immunochemical stain and the intensity of IGF-1 was determined by use of imaging analysis techniques. The apoptotic osteocytes in the trabecular bone of proximal femur were detected by using terminal-deoxynucleotidyl mediated nick end labeling (TUNEL) technique. Results The BMD of CON, Ex and OVX group was (0.170?0.011)g/cm2,(0.154?0.013)g/cm2 and(0.167?0.013)g/cm2, respectively. The difference of the BMD level between Ex and CON groups was not significant, however, the BMD level in OVX group was significantly lower than that of the Ex and CON groups (P

0.05 ). CONCLUSION: Shenmai Injection can increase the immunity function.