ObjectiveTo validate the judgment criteria of the validity scale for the assessment system on the character and temperament approach of command officers,and to describe the theory of this scale.MethodsUsing the way of imitating and field testing,correction of the criteria was verdicted based on the binary distribution theory.Results ①According the imitating research,about the 22 pairs of couple-items,the correct ratio,which is completely based on guessing,is about 4.9％,not reach 5％.②According the field testing,about the 22 pairs of couple-items,the correct ratio,which is completely based on guessing,is about 7.6％,not reach 8％.ConclusionOn the basis of theory analyzing,imitating research and field testing,not only can find the couple-items scale has many good features,such as more various show,more excellent disguise,but also can affirm the criterion of 19,which is based on the binary distribution theory,is also appropriate.

Objective To study the effect of millimeter wave radiation on human hepatoma cell. Methods BEL7404 hepatoma cells were cultured in vitro and randomly divided into 4 groups: the control group, the group radiated by millimeter wave for 30 min, the group treated with Fluorouracil(5 FU), and the group radiated by millimeter wave and treated with 5 FU simultaneously at same time. The ability of 35.8 GHz millimeter wave to induce the apoptosis of hepatoma cell was evaluated by analyses of fluorescence microscopy, DNA fragmentation assay and flow cytometry assay. Results BEL7404 cells radiated by the millimeter wave had the typical characteristics of apoptosis. Comparaed with the control group [(3.21? 1.06)%], the apoptosis rates were higher in 30 min radiating or/and 5 FU groups[ (14.33? 2.66)%, (18.58? 2.57)%, (27.91? 3.66)%]. Poly adp ribose polymerase(PARP) was found to be cleavaged in all the cells in millmeter wave radiation or/and 5 FU groups. Conclusion Radiation of 35.8 GHz could induce apoptosis of BEL7404 cell in vitro, and could act synergistcally with 5 FU treatment.

Objective To investigate the feasibility of L-[1- 13 C]phenylalanine breath test to assess liver function and determine the effective parameters of the test for quantitative assessment of liver function in adult. Methods Twelve healthy volunteers served as control group, and 26 liver cirrhotic patients with hepatitis B were divided into three groups, 10 patients in Child-Pugh classification A, 8 in B and 8 in C, An oral dose of 100 mg of nonradiative tracer L-[1- 13 C]phenylalanine were administered to all the subjects. Breath samples were taken before and different intervals within 360 min after administration. 13 CO_2 enrichment was measured by isotope ratio mass spectrometer. Results After the oral administration of L-[1- 13 C] phenylalanine, 13 C excretion reached a peak within 10-30 min. The parameters of 13 CO_2 excretion rate at 30 min ( 13 CO_2ER_ 30 ) , 13 C cumulative excretion of 60 min ( 13 C_ cum60 ), 75 min( 13 C_ cum75 ), 90 min( 13 C_ cum90 ) and 13 CO_2 half excretion time ( t _ 1/2 ) were shown sensitive, which could differentiate significantly the groups( P

Objective To study the correlation between small intestinal bacterial overgrowth (SIBO) and plasma endotoxin,platelet-derived growth factor(PDGF)level,and levels of serum hyaluronic acid (HA),laminin(LN),N-terminal procollagen Ⅲ peptide(PⅢNP)and type Ⅳ collagen(Ⅳ.C). Methods Lactulose hydrogen breath test (LHBT) was performed in 64 patients with chronic liver disease. Plasma endotoxin was measured with Limulus test, plasma PDGF was tested with ABC-enzyme-linked immunosorbent assay,serum HA,LN,PⅢNP and Ⅳ.C were tested by radioimmunoassay(RIA) in the patients. The relationship between SIBO and plasma endotoxin, PDGF and HA,LN,PⅢNP and Ⅳ.C levels were analyzed. Results Positive LHBT suggesting the existence of SIBO were observed in 22 patients(34.4%). Plasma endotoxin[(62?19) pg/ml vs.(31?13) pg/ml] and PDGF [(211?77) pg/ml vs.(136?57) pg/ml] levels were significantly higher in patients with SIBO than those in patients without SIBO (P

AIM: To observe the visual recovery and complications in patients with proliferative diabetic retinopathy(PDR)after vitrectomy combined with phacoemulsification.

METHODS: According to different surgical methods, 95 cases(95 eyes)with PDR were divided into the observation group(50 cases)and the control group(45 cases). The observation group was treated with vitrectomy and phacoemulsification, while the control group was treated with vitrectomy and lensectomy. The visual recovery, changes of intraocular pressure before and after surgery and the complications were compared between the two groups.

RESULTS: The visual acuity of the two groups was significantly improved after surgery(P<0.05), and the improvement in observation group was significantly greater than that in the control group(P<0.05). The intraocular pressure of the two groups was increased significantly at 1, 3 and 6mo after surgery(P<0.05), without significant difference between the two groups(P>0.05). The incidence of iris neovascularization and capsule opacification in the observation group(2%, 6%)were significantly lower than those in the control group(18%, 27%; P<0.05).

CONCLUSION: Vitrectomy combined with phacoemulsification can obviously improve the visual acuity of patients with PDR, and the incidence of complications is low. Although it will lead to an increase in intraocular pressure, it can be improved after symptomatic treatment.

AIM:To investigate the effect of visual training on the reconstruction of visual function in children with intermittent exotropia.

METHODS: A total of 94 children with intermittent exotropia diagnosed in our hospital from August 2016 to January 2019 were selected and 94 eyes were affected. According to whether the postoperative visual training was divided into two groups, 47 patients(47 affected eyes)were not visually trained after operation and were included in the control group; 47 patients(47 affected eyes)were visually trained 2wk after operation and were included in the observation group. Follow-up for 6mo was performed to observe the recovery of visual function and eye position regression.

RESULTS: Six months after surgery, the proportion of children in the observation group with simultaneous visual function(89%), fusion function(85%)and stereoscopic function(40%)was significantly higher than that in the control group(53%, 47%, 19%), and the orthotopic rate of children in the observation group was significantly higher than that in the control group(91% vs 66%).

CONCLUSION: The binocular visual function training after intermittent exotropia in children can promote the recovery of binocular visual function and reduce the risk of postoperative eye position regression.

The novel 8-Aminoquinoline(8-AQ) derivatives receptor was designed and synthesized.Its struc ture was characterized by NMR and ESI-MS.It was investigated to recognize metal ions such as Cu~(2+), Hg~(2+), Pb~(2+), Zn~(2+), Ni~(2+) and Cd~(2+) via its absorption and fluorescence spectra.The recognition mechanism .and bind ing mode were discussed.The results showed that 8-AQ derivatives 1 coordinated with Cu~(2+) can induce a new absorption peak at 509 nm, which turned the solution to red from colorless.In acetonitrile Hg~(2+) and Cu~(2+) induced dramatic enhancement in the fluorescence of the derivation 1 by 368 and 192 folds.Job plot showed 1:1 stoichiometry between 8-AQ and Cu~(2+) or Hg~(2+).

Objective To study the effect and reliability of venlafaxine on depression and anxiety symptoms in cervical cancer patient,and to assess the overall quality of life before and after treatment.Methods We treated 63 cervical cancer patients with depression by use of venlafaxine for 8 weeks,at the same time,evaluated their emotion state.change of quality of life and the adverse effect with HAMD,HAMA,TESS and laboratory tests.Results After 8 weeks.patients' scores of anxiety and depression decreased significantly from the baseline(P＜0.01),effective rates were 65%and 52%respectively.Three domains of quality of life(physiology,psychology and independence)became much better than those of baseline(P＜0.05).Conclusions This studyshows that venlafaxine can reduce the depression and anxiety symptoms in cervical cancer patients,and the react time is 2 to 4 weeks.Venlafaxine with the treatment of depression and enxiety can improve the quality 0f patients' life.

Objective To observe the impact of heart catheterization on blood and organ function,and create an stable animal model.Methods Ten male Wistar rats were divided into control group undergoing sham operation and experimental group undergoing improved heart catheterization(n=5 in each group).Blood samples were obtained every day from 10 rats before and after operation,and white blood cell(WBC),alanine aminotransferase(ALT),aspartate aminotransferase(AST),blood urea nitrogen(BUN),creatinine(Cr),creatine phosphokinase(CK) and lipopolysaccharide were detected.The pathomorphology of heart,liver and kidney in catheterized rats was observed on postoperative day 7.Results For the catheterized rats,blood cultures were negative of bacteria and the markers above were within normal range except for CK that recovered to normal value in 7 d,while the control rats had no obvious damage.Conclusion Heart catheterization causes no infection and organ function changes in rats.The animal model of heart catheterization for clinical pharmacological research is reliable.

OBJECTIVE:To isolate anti-endotoxin monomer component from Radix Paeoniae Rubra by means of biosen?sor technique.METHODS:The surface of biosensor cuvette was embedded by Lipid A;the screening target was established,tracking the silica gel column chromatogram and the binding ability of effluent component from HPLC with Lipid A with the ultraviolet scan result of the reclaimed material from biosensor as reference;anti-endotoxin monomer component was isolated;the component of monomer and the synthetic action of extrinsic lipopolysaccharides were also assayed by LAL test method.RESULTS:Components binding to Lipid A was reclaimed from cuvetee by biosensor technique,with the wavelength of UV absorption peak at194nm,215nm and275nm respectively.Anti-endotoxin monomers of higher binding activity with Lipid A isolated by HPLC method were1,2,3,4,6—O—pentagalloyl—?—D—glucose(PGG).PGG at concentration of8,4,2?g/ml respectively neutralized68.8%,43.7%and31.4%of LPS at an activity of0.1EU/ml respectively.CONCLUSION:It is fea?sible to isolate anti-endotoxin monomer component from Radix Paeoniae Rubra by means of biosensor technique,which is a fast,accurate and efficient and can be used to isolate anti-endotoxin monomer component from Radix Paeoniae Rubra on a large scale.