How to perform the scientific research and training in domestic hospital pharmacy is proposed in present paper. The proposal is in connection with the present stutus of domestic hospital pharmacy and for promoting its development. The significance of scientific research and training is described with emphasizing the points of attention. The general procedure of scientific research and writing of research papers are discussed. The problems that should be deliberated in the field of pharmaceutics, clinical pharmacy and drug therapeutics are more thoroughly enumerated in this paper. On the epuipment of hospital pharmacy, the elopment in mechanization and automation is emphasized. Thus, a good working condition can be established to help specialized construction and to promote the raising of scientific and technical levels.

This paper evaluates the relation between development of hospital pharmacy and modern techenical advance and presents the law of development of hospital pharmacy. We suggest that the available achievements in scientific research should be ac tively utilized so as to bring about a further advance in hospital pharmacy.

AIM: To establish equal proportion twice mixing method and make the mixing rationalized. METHODS: Advance basic configuration about the mixing method was brought forward and then studied on the mixing method by means of mathematics illation and experiment validated. RESULTS: The mixing method has been established and proved effective by experiment. CONCLUSION: The mixing method is a special mixing way that is reasonable and economy that can be used in industrial scale.

For observation on clinical therapeutic effect of modified Wei Er Fang on gastric precanceraus lesion (GPL), 90 cases of GPL were divided into 3 groups, the treatment group treated with modified Wei Er Fang, the control group I treated with Wei Fu Chuan and the control group I with Bismuth subcitrate, 30 cases each group. Results indicated that the total effective rate for clinical symptoms was 96. 67% in the treatment group which was significant different from 60. 00% in the control group I (P 0. 05), but the therapeutic effect on heterotypic proliferation in the treatment group was superior to those in the two control groups. It is showed that modified Wei Er Fang has a better action of reversing gastric precancerous lesion.

Objective To investigate clinical results of bilateral triceps bradhii approach in Gartland Ⅲ supracondylar fracture of humerus in children by comparing with reduction interne and plaster immobilization. Methods 102 children with Gartland Ⅲ supracondylar fracture of humerus,4 ～ 13 years old,from May 2003 to May 2009,were randomly divided into operation group and control group,51 cases separately. The operation group was treated by bilateral triceps bradhii approach, while control group was treated by reduction interne and plaster immobilization. All patients were made function exercise 4 weeks later,X-ray check periodically and followed up about 16 months with angle patronner and elbow joint function recorded. According to Flynn clinical function criteria,all patients were classified and analyzed. Results After follow-up and elbow joint function evaluation,48 cases ( 94. 1% ) show excellent and good results,3 cases(5.9% ) fair and poor in operation group,32 cases(62. 7% ) and 19 cases(37. 3% ) in control group. The differences between two groups was statistically singnificant ( P < 0. 05). Conclusion Comparing with reduction interne and plaster immobilization, bilateral triceps bradhii approach in Gartland Ⅲ supracondylar fracture of humerus in children has good clinical results and may be more effective therapy.

Objective To assess the protective effect of mastoparan-1(MP-1) on acute lung injury in mouse model with endotoxemia(ETM) induced by lipopolysaccharide(LPS),and to investigate the possible mechanism of protcetive effect.Methods The endotoxemia murine model was reproduced by tail vein injection of LPS(5mg/kg) in mice.Animals were randomly divided into normal control group(n=8),endotoxemia group(n=48) and MP-1-treatment group(MP-1 was injected in 3mg/kg at the same time of LPS injection,n=48).Animals of the latter two groups were sacrificed at 2,6,12,24,48 and 72 hours after injection,and then the blood and lung tissue samples were collected.Plasma LPS was assayed using kinetic turbidimetric limulus test,TNF-? and IL-6 were measured by appropriate ELISA kits,TLR4,TNF-? and IL-6 mRNA expressions in lung tissues were analyzed by real-time RT-PCR,myeloperoxidase(MPO) activity of lung tissues was determined by spectrophotometric method,and the pathological changes in lung tissues were observed under microscope.Result The plasma levels of LPS,TNF-? and IL-6 in the mice of endotoxemia group were increased at 2-48 hours after LPS injection(P

ObjectiveTo study the characteristics of the relationship between injury and disease in foren-sic identification cases of cervical trauma with cervical vertebra degeneration, and to explore the prob-lems about how to identify the participation rates of injury and disease using the clinical information, forensic examination and imaging examination.MethodsSeventeen forensic identification cases of cervi-cal trauma with cervical vertebra degeneration were collected. The age distributions, injury formations, injury severities and imaging findings of these cases were analyzed and the relationship between injury and disease was evaluated comprehensively.ResultsMiddle-aged and elderly were common in 17 cases and every case was involved with intervertebral disc herniation. The main reasons of injuries were hy-perextension. The degree of injury severity and vertebra degeneration were graded according to the imag-ing findings. The participation rates of injury and disease were also calculated comprehensively.Conclu-sionThe forensic identification cases of cervical trauma with cervical vertebra degeneration should be evaluated with clinical information, forensic examination and imaging finding.

In order to explore the mechanism of cardiac damage by PTH, the release of PGI2 from heart cells was measured. The results showed that bPTH ( 1-34) increased the release of 6-keto-PGFla from heart cells in a dose dependent manner. Calcium inophone A23187 also increased the 6-keto-PGF1? release, while EDTA and verapamil reduced it. These suggest that the PGI2 synthesis in heart cells was affected by intra and extracellular calcium. The significance of bPTH( 1-34) induced increase of PGI2 synthesis might be associated with interference of energy metabolism, and then, cell damage.

Objective To investigate the protective effect of exercise on osteopenia female rats caused by ovariectomy and its mechanism. Methods Twenty-four female rats aged 6 months were randomly divided into 3 groups: control group(CON),ovariectomized model group(OVX) and exercise group(Ex).The ovariectomy operation was performed with rats in OVX and Ex groups. Rats in Ex group were trained on a treadmill at a velocity of 18m/min for 1h, once daily for 5 days a week. Each group of rats were sacrificed at 12 weeks postoperatively and Dual-energy X-ray absorptiometry was carried out to measure the bone mineral density(BMD) of the femur. IGF-1 proteins in trabecular bone of proximal femur were examined by using immunochemical stain and the intensity of IGF-1 was determined by use of imaging analysis techniques. The apoptotic osteocytes in the trabecular bone of proximal femur were detected by using terminal-deoxynucleotidyl mediated nick end labeling (TUNEL) technique. Results The BMD of CON, Ex and OVX group was (0.170?0.011)g/cm2,(0.154?0.013)g/cm2 and(0.167?0.013)g/cm2, respectively. The difference of the BMD level between Ex and CON groups was not significant, however, the BMD level in OVX group was significantly lower than that of the Ex and CON groups (P

Objective To investigate the effects of IL-6/STAT3 activation on the proliferation of biliary epithelial cell following liver transplantation in rat.Methods Based on the classic "two-cuff" technique for orthotopic liver transplantation in rat,end-to-end anastomosis between the common hepatic arteries of donor and recipient by the modified "Gao stent" was performed to reconstruct hepatic artery blood flow.Wistar rats were then randomly divided into CP1h group and CP12h group(grafts were preserved in UW solution at 4℃ for 1h and 12h respectively),RPM group(CP12h group treated by rapamycin,RPM)and C group(control).At 1,3,7,14d after operation,liver IL-6 mRNA expression was analyzed by real-time RT-PCR.STAT3 activation was determined using laser confocal scan microscopy(LSM).BEC proliferation was detected by immunohistochemistry.Results In the CP1h group,expression of IL-6 mRNA was upregulated one day after operation(0.41?0.03),and then fell to the C group level subsequently(0.28?0.03,0.23?0.03 and 0.27?0.05 at 3,7 and 14d after operation).STAT3 activation in the BEC(94?8,61?6,39?4 and 34?3)and proliferation of the BEC following liver transplantation(2.1%?0.3%,5.9%?0.5%,2.6%?0.5% and 2.3%?0.5%)showed a similar trend.Compared with the CP1h and C group,the CP12h group demonstrated a significant and persistent up-regulation of IL-6 mRNA(0.60?0.03,0.73?0.02,0.38?0.02 and 0.30?0.04)and STAT3 activation(167?17,247?13,110?9 and 74?8).BEC proliferation in CP12h group were 7.0%?0.5%,27.8%?1.8%,23.1%?1.6% and 17.8%?1.2%,there was a direct correlation between IL-6 mRNA expression and STAT3 activation(r=0.95,P