Objective To study the effect of homocysteine(Hcy) on the expression of c-fos and c-jun mRNA and neointimal hyperplasia in rat common carotid artery, and to explore the possible mechanism of homocysteine exacerbating neointima formation after balloon injury. Methods The mRNA expression of c-fos and c-jun were detected by semi-quantitative RT-PCR in the common carotid artery. The morphology of arterial intima and media was studied by optical microscopy and image analysing system. Results The mRNA expression of c-fos and c-jun was significantly elevated in the low methionine(LM)〔(1.40?0.21),(1.43?0.25)〕 and high methionine(HM) 〔(1.68?0.27),(1.71?0.30)〕 than that in the control group 〔(1.10?0.15),(1.00?0.13), P

Objective To investigate the up regulation of captopril on the platelet nitric oxide(NO) synthase and clinical application in patients with ischemic stroke.Methods The washed platelets in vitro of the patients with acute ischemic stroke and healthy controls were added with oxidized LDL(oxLDL) and captopril followed by measurement of platelet NO synthase activity.Plasma oxLDL,NO sythase,NO and platelet NO sythase,NO in 40 patients were measured before oral captopril and after oral captropril 1 month,3 months.Results (1)After the washed platelets in vitro were added with oxLDL,the platelet NO synthase activities of the patients and controls were all significantly decreased( P

OBJECTIVE：To c ompare the difference of volatile oil and fatty oil constituents from Cinnamomum migao in different sources. METHODS ：The steam distillation method and Soxhlet extraction mothod were used to extract volatile oil and fatty oil from C. migao in different sources respectively ，and the extraction rates were calculated ；GC-MS was used to analyze volatile oils and fatty oils constituents from C. migao in different sources. The compounds were searched and matched through NIST 17，WILEY 275 databases and mass spectrometry computer date system. The relative percentage content of each constituent was calculated by peak area normalization method. RESULTS ：The extraction rates of the volatile oils from 4 batches of C. migao in different sources were 3.1％，4.5％，6.2％ and 5.5％，respectively；the extraction rates of the fatty oils from C. migao were 6.2％，8.3％，10.5％ and 9.4％，respectively. A total of 87 constituents were identified in 4 batches of volatile oils of C. migao in different sources ，of which 104 constituents were separated from S 1，67 were identified ，and the relative percentage content was 90.172％；102 constituents were separated from S2，73 were identified ，and the relative percentage contentwas 88.836％；77 constituents were separated from S 3，57 were identified ， with a relative percentage content of 93.972％；87 constituents were separated from S 4，60 were m identified，with a relative percentage content of 95.247％ . Among above 87 constituents，48 were monotyloids and their derivat ives，33 were sesquiterpenoids and their derivatives ，4 were aliphatic and 2 were ketones. There were 44 common constituents from the volatile oil of C. migao in different sources ，all of which were terpenoids. The relative percentage content of S 1-S4 were 38.556％，66.776％，88.886％ and 90.115％，respectively. Among 44 common constituents ，the relative percentage content of which were all greater than 1％ were 1，8-cineole（S2： 6.518％；S4：3.850％；S3：1.655％；S1：1.475％；），4-terpineol（S2：1.591％；S4：1.384％；S3：1.193％；S1：1.182％）， α-terpinenol（S3：8.662％；S4：7.173％；S2：6.503％；S1：4.839 ％），δ-cadinene（S3：8.597％；S4：5.329％；S2：2.677％； S1：2.547％），elemol（S3：4.781％；S2：4.113％；S1：2.568％；S4：1.897％）and γ-eudesmol（S2：4.061％；S3：2.167％；S1： 1.575％；S4：1.197％）. A total of 37 constituents were identified in the 4 batches of fatty oil of the C. migao in different sources ， of which 87 constituents were separated from S 1，34 were identified ，and the relative percentage content was 91.072％；69 constituents were separated from S 2，28 were identified ，and the relative percentage content was 90.527％；63 constituents were separated from S 3，23 were identified ，the relative percentage content was 85.297％；71 constituents were separated from S 4，24 were identified ，with relative percentage content of 91.527％. Among above 37 constituents，there were 21 monoterpenes and their derivatives，2 sesquiterpenes，13 aliphatics，and 1 alkane. There were 20 common constituents in fatty oil from C. migao of different sources ，and the relative percentage content in S 1-S4 were 89.667％，89.595％，84.651％ and 90.972％，respectively. Among 20 common constituents ，the constituents with relative percentage content greater than 1％ were methyl caprate （S4： 59.498％；S1：58.733％；S2：57.552％；S3：26.423％）and methyl dodecanoate （S3：31.434％；S2：26.990％；S1：25.095％； S4：24.334％）. CONCLUSIONS ：There are differences in volatile oil and fatty oil constituents of C. migao from different sources ， and the contents of the same constituent were also different.

Objective: To establish a UPLC-MS/MS analysis method for determination of baicalin, geniposide, chlorogenic acid, cholic acid and hyodeoxycholic acid in Qingkailing (lyophilized) for injection in rat plasma, and to investigate the pharmacokinetic behavior of this preparation in normal and cerebral ischemic rats. Method: Rats were randomly divided into normal group and cerebral ischemia model group. The rat model of cerebral ischemia was established by suture embolization. The rats were given by intraperitoneal injection, and normal saline was used as the solvent. Blood samples were taken at the corresponding time points. After treatment, UPLC-MS/MS was used to determine the blood concentration of five components. The main detection conditions were mobile phase of 0.1%formic acid aqueous solution-acetonitrile for gradient elution (0-0.25 min, 90%A; 0.25-1 min, 90%-75%A; 1-2 min, 75%-50%A; 2-2.6 min, 50%-45%A; 2.6-2.65 min, 45%-90%A; 2.65-4.0 min, 90%A), the flow rate of 0.4 mL·min^-1, the column temperature at 40℃, electrospray ionization under negative ion mode. The pharmacokinetic parameters were fitted and the bioavailability was calculated, the differences of treatment process of five components from Qingkailing (lyophilized) for injection in normal and cerebral ischemic rats were analyzed. Result: Compared with the normal group, the area under the curve (AUC_0-t) of geniposide in rats from cerebral ischemia model group decreased significantly after intraperitoneal injection of Qingkailing (lyophilized) for injection (P<0.05), and the time to peak (T_max) of chlorogenic acid in rats from cerebral ischemia model group was significantly earlier than that in the normal group (P<0.01). Pharmacokinetic parameters of baicalin, cholic acid and hyodeoxycholic acid had no significant difference between these 2 groups. Conclusion: Qingkailing (lyophilized) for injection has a certain difference in the treatment process between normal and cerebral ischemic rats, which has certain guiding significance for the clinical treatment of cerebral ischemic diseases with this preparation.