The paper was designed to consider the effect of vitamin E on metabolism of cyclic nucleotide in cold exposed male rats. The urinary excretion of cyclic nucleotide was investigated by radioimmunoassay after male rats treated with vitamin E in 4℃ exposure. The results showed that urinary excretion of cyclic nucleotide had been increasing and cAMP/cGMP ratio had been not changing in cold exposed male rats. When one week of the experiment vitmin E could increase urinary cAMP excretion more in its treated group than cold exposed group alone (P

Objective:To study the relationship between MMPs and the metastasis and the malignant degree of tumors in children.Methods:The expression of MMPs in different tumor groups was measured by means of immuno-histochemistry.Results:The expressions of MMP-3 in cytoplasm of tumor cells were observed.There was significant difference( P

Animals ; Diagnosis, Differential ; Humans ; Idiopathic Interstitial Pneumonias ; pathology ; Lung ; diagnostic imaging ; pathology ; Lung Diseases, Interstitial ; classification ; pathology ; Pulmonary Fibrosis ; pathology ; Tomography, X-Ray Computed

Animals ; Antigens, Neoplasm ; genetics ; metabolism ; Biomarkers, Tumor ; genetics ; metabolism ; Brain Neoplasms ; metabolism ; Cell Adhesion Molecules ; genetics ; metabolism ; Digestive System Neoplasms ; metabolism ; Female ; Genital Neoplasms, Female ; metabolism ; Glioma ; metabolism ; Head and Neck Neoplasms ; metabolism ; Humans ; Immunotherapy ; Male ; Prostatic Neoplasms ; metabolism ; Signal Transduction

Adenine ; analogs & derivatives ; therapeutic use ; Antiretroviral Therapy, Highly Active ; methods ; HIV Infections ; drug therapy ; prevention & control ; Hepatitis B, Chronic ; complications ; Hepatitis C, Chronic ; complications ; Humans ; Lamivudine ; therapeutic use ; Organophosphonates ; therapeutic use

Crisis Intervention ; Disasters ; Humans ; Stress, Psychological ; prevention & control

China ; Humans ; Mental Health ; Public Health Practice

Brain ; metabolism ; Brain Chemistry ; Creatine ; deficiency ; Humans ; Syndrome

Aim To investigate whether osthole can alleviate neuropathic pain induced by HIV gpl20 and its mechanism. Methods The paw withdrawal threshold and the paw withdrawal latency were observed to assess pain behaviors in four groups of the rats, including sham group, sham combined with osthole treatment group, gpl20 treatment group, and gpl20 combined with osthole treatment group. The protein expression levels of the P2X3 receptor, tumor necrosis factor-a receptor (TNF-aR), ERK, p-ERK in the L4-L6 dorsal root ganglia (DRG) were measured by Western blot. The mRNA expression level of P2X3 receptor was assessed by real-time quantitative polymerase chain reaction ( qPCR). The whole path clamp recording was used to measure HEK293 cell current activated by ATP. Results Osthole attenuated the mechanical and thermal hyperalgesia in gpl20 treated rats and down-regulated P2X3 receptor mRNA and protein expression in L4-L6 DRGs of gpl20 treated rats. Additionally, osthole simultaneously decreased the expression of TNF-ctR protein in L4-L6 DRGs and inhibited the phosphorylated ERK1/2 protein expression. Moreover, osthole reduced ATP activated current of HEK293 cells transfected with hP2X3R. Conclusion Osthole decreases the mechanical and thermal hyperalgesia induced by gpl20 through inhibiting P2X3R in DRG.

Objective To investigate the effects of DNaseⅠ-treated streptococcal antigens on peripheral blood mononuclear cells(PBMC)proliferation in psoriasis patients.Methods PBMC from 15 patients with psoriasis and 10 healthy controls were stimulated with three concentrations(2,10,25?g/mL) of streptococcal antigens(SA), nd DNaseⅠ-treated streptococcal antigens(DNaseⅠ-SA).After 72 h of culture,cellular proliferation was determined by ~3H thymidine incorporation.Results The DNaseⅠ-SA,at all the concentrations tested,was less potent than the corresponding concentrations of SA(P0.05),however,the lower concentrations(2,10?g/mL) of DNaseⅠ-SA were less potent than those of SA(P0.05).Also,the extent of proliferation response to SA and DNaseⅠ-SA was higher in the patients than in the controls(P