Objective To observe the effectiveness of high-intensity focused ultrasound(HIFU)in the treatment of lichen sclerosus of vulva.Methods 63 patients with lichen sclerosus of vulva between July 2005 to December 2005 were divided into two groups at random,ultrasound group and control group.Patients in ultrasound group were treated with HIFU.Those in control group were treated with hormone.Results The scores of itch had difference after treatment in both group(P

Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Pruritus Vulvae ; therapy

Bronchiolitis obliterans(BO) is chronic airflow obstruction syndrome characterized by small airway obstruction and airflow limitation.In children, BO is secondary to severe infection of respiratory tract, and its development is related with inflammation and immune response.At present, the diagnosis of BO mainly is depended on the clinical manifestation, lung function and examination results of high resolution computed tomography (HRCT) because of difficulty of lung biopsy.Pulmonary HRCT is a non-invasive examination, and worthy of generalization.Currently, BO has no accepted effective treatment,early diagnosis and treatment can block the progression of BO.Due to lack of realization of BO, doctors and parents fail to give enough attention, BO has a prolonged course and very poor prognosis.

Objective: To research the comparability of water vapor transmission of laminated films for pharmaceutical packaging determined by different instruments, methods and experimental conditions to provide reference and advice for the related inspection personnel.Methods: The principle, test parameters, conditions and scope of application for the testing methods for water vapor transmission in the national standard (GB) and international standards (ISO, ASTM) were compared by using three kinds of instruments based on different testing principles.Results: Different temperature and humidity conditions had great influence on the results of the same instrument.Under the same temperature and humidity, the instrument with different principles had little influence on the test results.Conclusion: Testing personnel should regularly use the standard membrane to calibrate the instrument of the sensor method.The temperature and humidity should be strictly controlled during the test.They should take measurement accuracy, personnel operation and economic loss into consideration to select the suitable method.

BACKGROUND: Rat model of orthotopic liver transplantation is a very valuable model for experimental study in liver transplantation including organ preservation, tissue ischemia-reperfusion injury, allograft rejection and immune tolerance mechanism. Stable liver transplantation animal model is the basis of the related experimental studies. However, its experimental operation is long and boring, especially performed by a single operator under direct vision. OBJECTIVE: To investigate the operation techniques to establish a stable rat model of orthotopic liver transplantation by a single operator under direct vision. METHODS: The orthotopic liver transplantation was performed using two-cuff method in 50 pairs of rats. We exposed the abdominal cavity fully, perfused the donor liver through abdominal aorta without flipping donor liver; suprahepatic inferior vena cava was in vivo cut down using one-step method, without diaphragm ring; the suprahepatic inferior vena cava was anastomosed with single-row suture, and the cuff of portal vein was installed by fixing the blood vessel forceps on rubber. Hepatic artery was not reconstructed. Fluid replacement was administered to maintain hemodynamic stability in rats after operation. RESULTS AND CONCLUSION: The donor operative time was (36.2± 2.5) minutes, donor liver trimming time was (12.2± 1.5) minutes, receptor operative time was (45.6 ± 3.5) minutes, suprahepatic inferior vena cava anastomosis time was (10.1 ± 2.1) minutes, portal vein cuff time was (1.5 ±0.9) minutes, infrahepatic inferior vena cave cuff time was (1.1 ± 0.6) minutes, anhepatic phase was (15.1 ± 2.2) minutes. The success ratio of the operation was 100% and the survival rates within 1 week and 1 month were all 100%. It is indicated that the key factors of a successful model were stable anesthesia, good donor liver perfusion, adequate exposure, skilled microsurgical technology and vascular anastomosis technique.

Objective To explore the expression of drug transporter organic anion transporting polypeptide2(Oatp2) and P-glycoprotein(P-gp) in brains of chronic epileptic rats induced by lithium-pilocarpine.Methods Chronic epilepsy modles were elicited after status epilepticus(SE) induced by lithium-pilocarpine in adult Wistar rats.The expressions of Oatp2 and P-gp were detected by immuno-histochemistry(SABC) method.Results Positive expression of Oatp2 was predominantly in the plexus epithelial cells and(blood-brain) barrier endothelial cells.Weak staining for Oatp2 was also detected in the neurocyte.In contrast,apparent expression of(P-gp) was seen in brain capillary and neurocyte only.Compared with normal control rats,expression of Oatp2 on the plexus epithelial cells and brain capillary in chronic epileptic rats was significantly decreased(P

Multidrug resistance to chemotherapeutic drugs is an important reason for clinical chemotherapy failure. The mechanisms of the classical drug resistance such as P-gp, MRP have been clarified. Hence, it is very important to further study the non-classical mechanism of multidrug resistance. We introduced the effects on MDR, the possible mechanisms of glutathione and related enzymes, as well as the relationship between structure of GSH analogues and the transport activity in this paper.

Objective To study the mechanism of high intensity focused ultrasound (HIFU) and discuss its clinical significance in the treatment of mid-late stage liver cancer. Methods The patients with mid-late stage liver cancer were treated with HIFU. The changes of photography, pathology and immunology after operation were evaluated. Results It was shown that the results of the photographic, pathologic and immunologic examinations changed during the treatment of the patients with HIFU. Conclusion HIFU is effective and safe for the treatment of mid-late stage liver cancer.

This study reviews the researches on self-handicapping in about more than 20 years. It summarizes the practical researches of self-handicapping from 7 aspects: (1)manifestations and types of self-handicapping behaviors;(2)evaluative conditions that typically elicit self-handicapping;(3)motives underlying self-handicapping; (4)factors influencing on self-handicapping; (5)consequences of self-handicapping; (6) measurements of self-handicapping. On this basis, it raises some questions in self-handicappping researches and points out the future direction.