1.RNAi-induced down-regulation of STAT3 expression promotes the apoptosis of Bel-7402 Hepatocarcinoma
Junwei ZHANG ; Yi ZHANG ; Jiajun DU ; Qi LIU
Journal of Chinese Physician 2013;15(9):1195-1198
Objective To explore influence of JAK/STAT3 signaling pathway on the apoptosis of HCC cells.Methods DNA-vector-based RNAi approach silence was used to down-regulate STAT3 expression in Bel-7402 cells.According to the STAT3 cDNA sequence in the GeneBank database,the plasmid pGCsi.U6/neoRFP-STAT3 that was designed for expression of STAT3 siRNA was constructed and synthesized,and then transfected into the Bel-7402 cells with iipofectamine 2000.The apoptotic rate was measured with flow cytometry (FCM) and annexinV/PI apoptosis detection kit staining.The mitochondrial membrane potential (BΨm) was visualized by the JC-1 fluorescence staining and the inverted fluorescence microscope.Moreover,the expression of caspase-3 protein was analyzed by Western blotting.Results The apoptotic ratio of STAT3-siRNA group was (38.82 ± 0.88) %,which was significantly higher than that in other group [control group (9.22 ± 0.38) %,scramble-siRNA group (16.47 ± 1.04) %,P < 0.05].The mitochondrial membrane potential of STAT3-siRNA group observed by the JC-1 fluorescence staining was decreased significantly[(91.33 ± 1.78) %] and [(89.90 ± 1.92) % vs (59.06 ± 1.89) %,P < 0.05].The Western blot results showed that the protein expression of active caspase-3 in STAT3-siRNA group was significantly higher than other groups (0.48 ± 0.05 vs 0.22 ± 0.04 and 0.26 ± 0.06,P < 0.05).Conclusions STAT3 gene silencing significantly improves the apoptotic effect in the Bel-7402 ceils.
2.Research on carotid artery wall elasticity using echo-tracking in children
Yi AI ; Jianguang QI ; Junbao DU ; Wanzhen LI ; Yuli WANG
Chinese Journal of Ultrasonography 2003;0(07):-
Objective To assess the wall elasticity of the common carotid artery using echo-tracking in healthy children and children with hyperlipidemia.Methods The study included 68 normal healthy children and 25 children with hyperlipidemia.All the subjects were 3-18 years old.They were divided into three groups according to ages 3-6 years old group,7-13 years old group and 14-18 years old group.Echo-tracking was used to measure the pressure-strain elastic modulus (E?),stiffness parameter(?)and arterial compliance (AC) of the common carotid artery.Results The average values of E? were different among the three age groups in normal healthy children (all P
3.Acupuncture method of "Huoxue Sanfeng, Shugan Jianpi" for morning blood pressure in patients with cerebral infraction combined with essential hypertension: a randomized controlled trial.
Xinxin GAO ; Fen MA ; Qi ZHAO ; Yi ZHANG ; Yuzheng DU
Chinese Acupuncture & Moxibustion 2016;36(5):459-462
OBJECTIVETo observe the clinical effects of acupuncture method of "Huoxue Sanfeng, Shugan Jianpi" (activating blood and eliminating wind, soothing liver and strengthening spleen) on morning blood pressure in patients with cerebral infraction combined with essential hypertension.
METHODSSixty-eight patients were randomly divided into an observation group and a control group, 34 cases in each one. The patients in the two groups were treated with acupuncture method of "Xingnao Kaiqiao" (consciousness-restoring resuscitation) and oral administration of nifedipine. In addition, patients in the observation group were treated with acupuncture method of "Huoxue Sanfeng, Shugan Jianpi" that met the criteria of standard manipulation, in which bilateral Renying (ST 9), Quchi (LI 11), Hegu (LI 4), Zusanli (ST 36) and Taichong (LR 3) were selected. The treatment was given once a day, five times a week, for totally six weeks. The improvement and control rate of morning blood pressure in the two groups were observed.
RESULTS(1) After treatment, the morning blood pressures were decreased significantly in the two groups (all P < 0.05); after 15 treatments, the reduction of systolic pressure and diastolic pressure in the observation group was superior to that in the control group, but the difference was not significant (both P > 0.05); after 30 treatments, the reduction of systolic pressure and diastolic pressure in the observation group was significantly superior to that in the control group (both P < 0.05). (2) After 30 treatments, the control rate of morning blood pressure in the observation group was significantly higher than that in the control group [82.4% (28/34) vs 58.8% (20/34), P < 0.05].
CONCLUSIONAcupuncture method of "Huoxue Sanfeng, Shugan Jianpi", characterized with standard manipulation criteria, can effectively control morning blood pressure in patients with cerebral infraction combined with essential hypertension.
Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Blood Pressure ; Cerebral Infarction ; physiopathology ; therapy ; Essential Hypertension ; Female ; Humans ; Hypertension ; physiopathology ; therapy ; Liver ; physiopathology ; Male ; Middle Aged ; Spleen ; physiopathology ; Treatment Outcome
4.Skin prick test of inhalative allergens for patients with allergic rhinitis in Yichang.
Long ZHANG ; Bei HAN ; Zhimao ZHANG ; Airong LIU ; Guobin LIU ; Zhimei DU ; Yi YAO ; Qi QI
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(2):98-101
OBJECTIVE:
To investigate the allergens in patients with allergic rhinitis in Yichang, so that to find common allergens of Yichang and to provide statistic basis for a reasonable prevention and treatment to allergic rhinitis.
METHOD:
1,979 patients with allergic rhinitis in Yichang were detected for allergens by skin prick test and the distribution of positive rates to inhaled allergens was compared between different genders and ages.
RESULT:
1,545 (78.1%) of 1,979 suspected allergic rhinitis patients presented positive reaction. The positive rate in male was significantly higher than in female, and that in juvenile group was significantly higher than in adults. Among positive cases in inhalation group, the most common allergen was flour mite (80.4%), followed by house dust mite (64.9%), cockroach (13.3%) and artemisia pollen (8.2%).
CONCLUSION
The study shows that the flour mite and house dust mite are the most common inhaled allergens causing allergic rhinitis in Yichang. We should pay more attention to the prevention and treatment for the juvenile patients.
Adolescent
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Adult
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Aged
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Allergens
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immunology
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Child
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Child, Preschool
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China
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epidemiology
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Female
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Humans
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Male
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Middle Aged
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Rhinitis, Allergic
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diagnosis
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epidemiology
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immunology
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Skin Tests
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Young Adult
5.High-throughput screening assay for groups of effective components extracted from Xiaoxuming Recipe
Yuehua WANG ; Haixia ZHANG ; Qi LI ; Yi DING ; Juanjuan HU ; Guanhua DU
Journal of Integrative Medicine 2006;4(1):64-7
OBJECTIVE: To present a new approach for the research of effective components extracted from compounds of traditional Chinese medicine and their action mechanisms by high-throughput screening assay. METHODS: We observed the anti-oxidation activities of 240 sequential components (L1-L120 and A1-A120) extracted from Xiaoxuming Recipe, the effects of these components on SH-SY5Y cells with H(2)O(2)-induced and L-glutamic acid-induced damages and the levels of resting calcium ion in neurocytes of rats. RESULTS: Some components (L1-L40, A100-A120) extracted from Xiaoxuming Recipe had the corresponding effects listed above. The combination of these components was regarded as the groups of effective components of Xiaoxuming Recipe in treating sequelae resulting from brain ischemia. CONCLUSION: Xiaoxuming Recipe has protective effect on brain-ischemia-induced damages through the actions of multiple components with multiple targets.
6.Infrared spectroscopic study on water processing of kusnezoff monkshood root
Xin YI ; Hong DU ; Mingxia WU ; Qi YU ; Yuefei HOU ; Wenwen ZHAI ; Suqin SUN ; Jingjuan WANG
International Journal of Traditional Chinese Medicine 2012;34(11):1000-1003
Objective Infrared spectroscopy was used to study on both raw material and different degree water processing varieties extract of Radix Aconiti kusnezoffii,to observe the changes of main toxic components in processing,and thus to improve the quality of Radix Aconiti kusnezoffii processed products.Methods Fu Liye transform infrared spectroscopy was adopted to study the infrared spectrum characteristic of raw materials and different degree water processing varieties of.Radix Aconiti kusnezoffii.Results ①Aconitine and hypaconitine infrared spectrum showed that 1717 cm-1,1727 cm-1 and 1711 cm-1 is the C=O stretching vibration peak.It is a diester alkaloids characteristic peaks; ② although absorption peak of all vesicular samples had a certain change,it still existed diester alkaloids absorption peak,indicating the incomplete hydrolysis; ③ boiled aconite processing methods demonstrated diester alkaloids absorption peak shift in the water sample.Conclusion Diester alkaloids in Radix Aconiti kusnezoffii shows a positive relation with its time soaked in water.
7.Rupture of primary splenic multilocular mucous cyst accompanied by pseudomyxoma peritonei: report of a case.
Wen QI ; Wen-xiu LIN ; Na DU ; Xiao WANG ; Li-yi GU ; Qian-xing ZHANG
Chinese Journal of Pathology 2013;42(2):129-130
Aged
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CDX2 Transcription Factor
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Cysts
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Female
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Homeodomain Proteins
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metabolism
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Humans
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Keratin-20
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metabolism
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Mucous Membrane
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pathology
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Peritoneal Neoplasms
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metabolism
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pathology
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surgery
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Pseudomyxoma Peritonei
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metabolism
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pathology
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surgery
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Rupture
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Splenic Neoplasms
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metabolism
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pathology
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surgery
8. Challenges and prospects for early gastrointestinal cancer screening in China
Academic Journal of Second Military Medical University 2020;41(1):1-5
China is a country with high incidence of gastrointestinal (GI) cancer. Gastric cancer, esophageal cancer and colon cancer seriously threaten the health of people, and leads to heavy medical burdens. This review discusses the current screening protocol of GI cancer and pancreatic cancer. It also interprets the relevant policies issued by the state in recent years, and evaluates the effects of new technologies such as serum pepsinogen combined with gastrin in detecting gastric cancer, fecal and blood gene detection for colon cancer, and "serum liquid biopsy" of pancreatic cancer for early cancer screening. We also point out the difficulties and challenges in cancer screening at early stage, and the significance of promoting early cancer screening to reduce the mortality of GI cancer in China.
9. Switch of focus on treatment of severe acute pancreatitis
Academic Journal of Second Military Medical University 2018;39(3):233-237
Severe acute pancreatitis is a common critical emergency in the digestive system. It is difficult to treat in clinic and has high mortality. According to the new Atlanta classification standard proposed in 2012, the treatment of acute pancreatitis mainly focused on early indentification, maintenance of organ function, anti-inflammatory response, intestinal function maintenance and other measures, so as to improve the success rate of the treatment. As more patients with severe acute pancreatitis have successfully crossed the acute phase, the prevention and treatment of subsequent complications of acute pancreatitis have been increasingly valued. To focus on the “secondary attack” of acute pancreatitis, it is necessary to carry out clinical research on the subsequent complications such as the accumulation of peripancreatic fluid, infectious necrosis, and vascular complications, and to reduce the mortality. This is a change in the focus of acute pancreatitis treatment and a further extension of the concept of multidisciplinary collaboration in acute pancreatitis.
10.Preparation of oral Helicobacter pylori HpaA nucleic acid vaccine with interlukin-2 as adjuvant to prevent Helicobacter pylori infection
Can XU ; Zhao-Shen LI ; Yi-Qi DU ; Zhenxing TU ; Yanfang GONG ; Guoming XU ;
Chinese Journal of Digestion 2001;0(10):-
Objective To construct a nucleic acid vaccine expressing H.pylori HpaA and inter- leukin-2 gene and to identify the immunogenicity of the vaccine proteins in vitro and protection in vivo. Methods The H paA gene fragment was amplified by polymerase chain reaction(PCR) from the genomic DNA of the standard H.pylori strain 17874.Mouse interlukin(IL)-2 gene was amplified from pClneo- IL-2.The HpaA and IL-2 were cloned into pUCmT vector.After DNA sequences of the amplified HpaA gene and IL-2 were confirmed,both were cloned into the eukaryotic expression vector pIRES through a serial of enzyme digestion and ligation reactions.The recombinant plasmids were screened by PCR and restriction enzyme digestion.Then,recombinant pIRES-HpsA-IL-2 was transfected to COS-7 cells using Lipofectamine~(TM)2000.The immunogenicity of HpaA and IL-2 protein was detected by SDS- PAGE and Western blot.The recombinant plasmids were transformed to LB5000 and then to final host SL7207.The recombinant strains were passaged repeatedly.The mice were challenged with H.pylori after 4 weeks of inoculation of nucleic acid vaccine.H.pylori infection was detected by rapid urease test.Results The amplified HpaA gene fragment and IL-2 were confirmed by sequence analysis.The eukaryotic expression vector plRES and the pIRES-HpaA-IL-2 construction were confirmed by PCR and restriction digestion.The expressions of HpaA(30 000) and IL-2(14 000)protein by pIRES-HpaA-IL- 2 were detected by Western blot.The in vivo study showed that 75.0% and 58.4% of mice vaccinated by HpaA-IL-2 and HpaA,respectively,were protected anaigst H.pylory infection,which was signifi- cant different in comparison with PBS control (P