Objective To explore the role of neutrophil extracellular traps (NETs)-induced endothelial cell damage in the pathogenesis of dermatomyositis (DM)-associated interstitial lung disease (ILD).Methods Serum free DNA and krebs von den lungen-6 (KL-6) level were tested in healthy controls,dermatomyositis patients with or without interstitial lung disease (DM-ILD and DM-NILD).Subjects' peripheral blood neutrophils were stimulated with phorbol 12-myristate 13-acetate (PMA),then human umbilical vein endothelial cells (HUVECs) were co-cultured with NETs.The cell morphology was observed by the inverted phase contrast microscope.Cell viability was detected by cell counting kit-8 (CCK8).Results The concentration of serum free DNA in DM patients [(271.27 ± 76.53) μg/L] was significantly higher than that in health control (HC) [(152.89 ± 37.34) μg/L,P ＜ 0.001].Moreover,free DNA level in DM-ILD patients [(302.67 ± 74.15) μg/L] was higher than that in DM-NILD patients [(235.59 ±63.55) μg/L,P ＜0.005].The concentration of KL-6in DM patients [(3.08 ±2.07) μg/L] was higher than that in HC [(0.87 ± 0.51) μg/L,P ＜ 0.001].Similarly,KL-6 in DM-ILD patients [(4.00 ± 2.44) μg/L] was higher than that of DM-NILD patients [(2.03 ± 0.61) μg/L,P ＜ 0.005].Free DNA and KL-6 were positively correlated (r =0.251,P ＜ 0.05).The survival of endothelial cells in DM group (53 ± 11) ％ was lower than that of HC group [(70 ± 5) ％,P ＜ 0.001].Not surprisingly,the survival of endothelial cells in DM-ILD group (44 ±4) ％ was lower than that in DM-NILD group [(61 ± 8) ％,P ＜ 0.01].Conclusion NETs could play an important role in the pathogenesis of dermatomyositis associated interstitial lung disease,suggesting that NETs may be the potential therapeutic target.

AIM: To study the physiological function changes of the tears film after rebuilding ocular surface with corneal stem cells, and to discuss the validity and the estimate system of rebuilding ocular surface with the corneal stem cells. METHODS: The male New Zealand rabbits were used to establish the alkali burning model in the right eye. The corneal stem cells of the left eye were cultured on the amniotic membrane in vivo, and then transplanted to the right eye. Furthermore, the physiological function changes of the tear film were examined. RESULTS: Compared to the before alkali burning, the ocular surface cell morphology was similar after rebuilding ocular surface with the corneal stem cells, which were cultured on the amniotic membrane in vivo; The tear film breakup time test showed the a remarkable difference between after and before the alkali burning, but the cell modality after rebuilding had no remarkable difference compared to that before the alkali burning. CONCLUSIONS: It's an effective method to rebuild the ocular surface with the corneal stem cells in vivo, the cell framework and the physiological function of the tears film recover well after rebuilding. It may be a validity estimate system of rebuilding ocular surface to analyze framework and configuration of the ocular surface and test the tear film breakup time.

Certification ; China ; Humans ; Occupational Health Physicians

Certification ; Humans ; Occupational Health Physicians

Objective To study the relationship between magnesium level in umbilical vein and mother′s peripheral blood with intrauterine growth retardation (IUGR) and fetal weight. Methods 39 pregnant women with IUGR were randomly divided into 3 groups: Group 1 (n= 14): The patients were treated with 10% glucose 500 ml + danshen compound 14 ml + low molecular weight dextran 500 ml iv; Group 2 (n= 14): in addition to the same treatment as in group 1, 25% magnesium sulfate 20 ml in 5% glucose 500 ml iv was given; Group 3 (n= 11): no treatment was given; and another 12 normal term delivery women were served as control. Magnesium concentration levels were determined in both maternal peripheral blood and their fetal umbilical vein. Results Maternal serum magnesium level was higher in Group 2 (1 06?0 09) mmol/L than that in Group 1 (0 69?0 05) mmol/L (P

Objective: To analyze the prevalence of reduced visual acuity (VA) and to examine the association between reduced visual acuity and influencing factors among Chinese Han students, so as to provide bases for the prevention and treatment of reduced visual acuity.Methods: Subjects were from 2014 Chinese National Surveys on Students' Constitution and Health (CNSSCH).In this survey, 261 832 Han students aged 7-22 years participated.Unaided distance VA was measured using a retro-illuminated lgMAR chart with tumbling-E optotypes.Ametropia was determined by using serial bronchoscopy.Logistic regression was used to assess the association between reduced visual acuity and influencing factors.ArcMap 10 was used to map the regional distribution of reduced visual acuity.Results: Nationwide, the overall prevalence of reduced visual acuity was 66.6%, and was highest among the urban girls (73.5%).The prevalence of myopia was 60.8% and accounted for more than 90% among the students with reduced visual acuity.Compared with 2010 CNSSCH, the increments of reduced visual acuity were highest in the students aged 13-15 years (6.8 percentage points).The prevalence of reduced visual acuity ranged from 50.6% to 76.2% in each province (autonomous region, municipality) in 2014 CNSSCH.The top three of the prevalence of reduced visual acuity were Jiangsu (76.2%), Zhejiang (76.0%) and Shandong (75.9%), and the lowest three were Hainan (50.6%), Guizhou (53.9%) and Xinjiang (57.6%).Logistic regression showed that the girls, urban students, and students who spent ≥2 h per day in their after school homework were more likely to develop reduced visual acuity, but the students with physical activity time ≥2 h per day were less likely to develop reduced visual acuity.Conclusion: Although the situation was different in each province (autonomous region, municipality), reduced visual acuity has become a public concern affecting the health of students in China.Based on the intervention on the key populations, such as urban girls and pre adolescent students, we should also continue to strengthen the prevention and control for reduced visual acuity among all students.

Objective To define the loci of the mutant gene in the loop-tail mouse.Methods To study the heredity pattern, loop-tail mice were mated with normal C57BL/6J and C3H mice.Their offsprings with loop-tail or normal phenotype were registered respectively.Microsatellite marker D1Mit113 and D1Mit149 were used to locate the mutant gene.Based on fine mapping, the candidate gene Vangl2 was found.Vangl2 gene from the loop-tail mice was amplified by PCR followed by sequencing.Incision enzyme FspBI ( BfaI ) identified the genotype of offspring from loop-tail mice intercrossing.Results Heredity test indicated that the loop-tail phenotype was controlled by a single dominant gene not with 100%penetrance but was affected by genetic background.A C-to-T transversion was at the 1345bp in Vangl2 gene of the loop-tail mice.Conclusions The C-to-T transversion introduces a pre-termination codon of amino acids and causes the phenotype of loop-tail phenotype.None homozygous mice were found in the offsprings, suggesting that the homozygous mice are lethal.

Objective:To investigate the psychological state of the patients from Wenchuan Earthquake treated in some local and military hospitals.Methods:We consulted the general data and pathogenetic condition of 116 patients from the earthquake-stricken area of Sichuan,studied their mental health using the Hamilton Anxiety Scale(HAMA) and investigated their psychological changes during the rescue and treatment with self-designed questionnaires.Results:The earthquake patients manifested obvious anxiety in the initial stage and their mental health was gradually improved towards the end of the rescue process.Conclusion:Wenchuan Earthquake caused great psychological trauma in the earthquake patients,for whom timely psychological intervention is of utmost importance.

OBJECTIVE To analyze situation of clearence of rust in the surgical operation apparatus.METHODS 300 surgical operation apparatus were choosed,divided into the A,B,C groups.For group A,soak method was taken to clear the rust,group B with soak and hand scrub in method,group C with soak.hand scrub and lubricant method.RESULTS The method for group C was most effective.The qualified rate and re-rust rate were 99.0% and 2.0%,respectively.CONCLUSIONS Clearence is a better method for operation apparatus maintanance,but daily cleaness is also important to avoid rusting.

OBJECTIVE To analyze the cost performance of small packs of surgical instruments and to look for their optimizing sterilization schemes.METHODS 720 sample from total surgical instruments were sefested,pressuresteam sterilization ethylene Oxide and hydrogen peroxide plasma sferilization were pexformal and compared.Statistics and analyzing finally under the condition of each kind puts The sterilization effect,cost and the period were analyzed.RESULTS All three methods call guarantee the sterilization effect,but the cost of EO and plasma were more than those of pressuresteam method(452% and 960%).CONCLUSIONS Pressuresteam sterilization show advantages in small packing instruments.