Objective: To establish the quality standard for Ganmaoyangkeling Tablets(Radix Isatidis, Herba Solidaginis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, etc.). Methods: Radix Glycyrrhizae and Herba Solidaginis were identified by TLC, and the content of quercetinum was determined by HPLC. Results: Quercetinum shows a good linear relationship in the range of 0.05～0.40?g ( r =0.99997), and the average recovery is 101.9%, RSD is 2.07%. Conclusion: These methods is simple, accurate and specific and can be used for the quality control of Ganmaoyangkeling Tablets.

It is the requirement to change the utility reading of medical undergraduates and to realize the functions of medical academic library for medical academic library to popularize reading service .The serial reading populari-zation service practice was thus described in this paper with certain feasible suggestions put forward for the further reading popularization activities.

Attention Deficit Disorder with Hyperactivity ; epidemiology ; Child ; Comorbidity ; Humans

Attention Deficit Disorder with Hyperactivity ; diagnosis ; therapy ; Humans

Objective To study effect of celecoxib on Wnt/β-catenin signaling pathway and matrix metabolism of chondrocytes in osteoarthritis (OA).Methods Use a modified way of mechanical fixation to make rabbit OA model.Knee OA separated and extractd rabbit chondrocytes were and then culture in medium.The cultured chondroctyes were divided into the Dimethyl sulfoxide (DMSO) control group and the celecoxib group, which were given 1％ DMSO and 10 μmol/L celecoxib respectively.After 48 hours' intervention, the protein expression and gene expression of Wnt/β-catenin signaling pathway related factors, including β-catenin,matrix metalloproteinase (MMP)-3, MMP-13, collagen Ⅱ were detected.T-test was used for the comparison between the two groups.Results Compared with the control group, the levels of type Ⅱ collagen [(275±19) ng/ml vs (202±12) ng/ml, t=-6.21, P＜0.05] and proteoglycan [(58±12) ng/ml vs (30±3) ng/ml, t=-9.23, P＜0.05] significantly increased in celecoxib group (P＜0.05).Meanwhile, compared with the control group, the levels of β-catenin, MMP-3, MMP-13 mRNA and protein in celecoxib group were significantly reduced (P＜0.05).Conclusion In rabbit OA chondrocytes, celecoxib can inhibit Wnt/β-catenin signaling pathway, reduce downstream factors expression including MMPs, thereby increase the cartilage matrix type Ⅱ collagen and proteoglycan levels.

Objective To assess whether lysyl oxidase like-2 (LOXL2) has a predictive or prognostic value in pulmonary fibrosis mice,and identify the relationship between LOXL2 and transforming growth factor (TGF)-β 1.Methods Male C57BL/6 mice (n=50) were randomly divided into the experimental group (n=40) and controls (n=10).Each mouse in the experimental group was induced by injecting bleomycin into trachea and mice were sacrificed on day 3,7,14 and 28,respectively.The concentrations of LOXL2,TGF-β 1 of the serum and lung homogenates were measured by enzyme-linked immunosorbent assay.One-factor analysis of variance (ANOVA) and nonparametric rank sum test was used for the comparisons between the two groups.Associations between these factors were analyzed by Spearman's test.Results ① The levels of serum and lung homogenates LOXL2 in the experimental group were obviously increased as compared to those in the controls [serum:(56±48) vs (25±23),P＜0.05;lung homogenates:(61±32) vs (22±16),P＜0.05].And in the experimental group,a positive correlation was found between the level of LOXL2 and the radiological index,the pathological score of inflammation and fibrosis.(Serum:r=0.435,0.533,0.335,P＜0.05;lung homogenates:0.675,0.736,0.526,P＜0.05).② The levels of lung homogenates TGF-β 1 in the experimental group were obviously increased as compared to those in the controls [(302±197) vs (64±16),P＜0.05].A positive correlation was found between the level of lung homogenates LOXL2 and the level of lung homogenates TGF-β1 (r=0.520,P＜0.05).Conclusion LOXL2 participates in the pathological process of bleomycin induced pulmonary fibrosis in mice.It predicts pulmonary fibrosis in the preclinical stage and is associated with increased risk for disease progression.The elevated levels of LOXL2 and TGF-β 1 may have some relationship.They both are involved in the disease progression of pulmonary fibrosis.

OBJECTIVE:To observe the effect of the side-hole hollow bone cement nail on promoting bone fracture healing. METHODS:This experiment was performed in the Third Hospital of Hebei North College from July 2006 to December 2008. Femoral neck samples were obtained from 60 adult corpses of which the patients were dead one year at most with the mean age of 70 years. X-ray examination showed a significant osteoporosis,but no other abnormalities. Hollow nail was provided by Sanyou Medical Apparatus Co.,Ltd.,Shanghai; self-cohesion methyl methacrylate bone cement was provided by Dental Material Factory,the Second Medical University of Shanghai. In the maximal axial pulling-out force group,the hollow nails were directly implanted into 10 femoral neck samples after drilling at the axial center. The polymethyl methacrylate bone cement was inserted into other 10 femoral neck samples after drilling at the axial center,and the side-hole hollow nail were then implanted. The pulling out experiment was performed by AGS-10KN material testing machine to measure the maximal axial pulling-out strength of hollow nail and the maximal loading when the nail was loose during anti-bending cycle experiments. RESULTS:① The maximal axial pulling-out force of 10 femoral neck samples implanting the hollow nail was 471.5-1 070.5 N,with the mean value of (855.0 ? 207.5) N; the maximal axial pulling-out strength of femoral neck samples implanting the side-hole hollow nail added by poly-methyl methacrylate bone cement was 2 023.7-3 572.1 N,with the mean value of (2 943.3 ? 535.8) N. The maximal axial pulling-out strength in the combined group was significantly greater than hollow nail implantation group (P

Objective To compare the effects of invert sugar versus 10% glucose on the blood glucose concentrations of patients in neurosurgical intensive care unit .Methods This is a prospective ,randomized ,double blind and compared study .A total of 40 in-sulin-requiring neurosurgical critical patients were randomized to receive intravenous ,1 000 mL ,either 10% invert sugar (experi-mental group) or 10% glucose(control group) on their first day when they were admitted to the intensive care unit (ICU ) .Blood glucose concentrations were measured every four hours and liver and kidney function indicators were recorded respectively .We’ve also compared the difference of the blood glucose concentrations changes between the two groups .Results There were significant differences in the glucose concentration between the two treatment groups (P<0 .05) ,whereas no significant differences in the inci-dences of hypoglycemia ,renal failure and hepatic damage were observed .No allergic reactions were found in both groups .Conclusion 10% invert sugar is a better choice for neurosurgical critical patients who need regular insulin infusion to control blood glucose .

Objective To investigate the effect of mycophenolic acid (MPA) on the proliferation of rat pulmonary microvascular endothelial cells (PMVECs) and the effects of MPA on the secretion of endothelin-1 (ET-1) and interleukin-6 (IL-6).Methods PMVECs were cultured by tissue-sticking method.Cells were divided into 5 groups,named as the control group,low-dose MPA group,moderate-dose MPA group,highdose MPA group and super high-dose MPA group,according to the final concentration of MPA (0,0.1,1,10 and 100 μmol/L,respectively).The proliferation of rat PMVECs was detected by cell counting kit-8 (CCK-8).The ET-1 and IL-6 secretion were assessed by radio-immunoassay.One-way analysis of variance was used to detect the differences between the group means.Results The cultured PMVECs of rats in vitro showed fusiform or polygonal in shape,and the expressed factor Ⅷ associated antigen stain was positive.At 24 h,the A values of the 5 groups (from control group to super high-dose MPA group) were 1.15±0.20,1.20±0.13,0.88±0.20,0.62±0.05 and 0.56±0.10,respectively,there was no significant difference in the A values on 450 nm between the control group and the low-dose MPA group (P=0.388),as well as the high-dose MPA group and super high-dose MPA group (P=0.292),but there were significant differences between any other two groups (P＜0.01).At 48 h,the A values of the 5 groups (from control group to super high-dose MPA group)were 2.61±0.21,2.52±0.23,1.99±0.17,0.84±0.16 and 0.65±0.12,respectively,there was no significant difference in the A values on 450 nm between the control group and low-dose MPA group (P=0.094),but there were significant differences in the A values between any other two groups (P＜0.01).At 72 h,the A values of the 5 groups (from control group to super high-dose MPA group) were 2.62±0.24,2.62±0.19,2.37± 0.38,0.79±0.17 and 0.61±0.17,respectively,there was no significant difference in the A values on 450 nm between the control group and low-dose MPA group (P=0.931),but there were significant differences in the A values between any other two groups (P＜0.05).There were no significant differences in the ET-1 concentrations between the control group and low-dose MPA group (P=0.156),and high-dose MPA group and super high-dose MPA group (P=0.262),but there were significant differences in the concentrations between any other two groups (P＜0.01).There were no significant differences in the IL-6 concentrations of the five groups between any of the two groups (P＞0.05).Conclusion MPA can inhibit fetal bovine serum induced rat PMVECs proliferation and the secretion of ET-1 in a dose-dependent manner.

Objective To investigate the relationship between plasma anti-β2-glycoprotein I (anti-β2-GP I )and cardiovascular disease (CVD) in systemic lupus erythematosus (SLE).Methods Eighty-onepatients with SLE [the mean age was (45±18) years old,among whom 73 were female and 8 were male] and20 controls [the mean age was (43±17) years old,among whom 14 were female,and 6 were male] wereenrolled.Plasma anti-β2-GP I was measured by ELLSA.The relationship between plasma anti-β2-GP I level and CVD in SLE patients was investigated with Logistic regression model.T-test,x2 test,Spearman's correlations and Logistic regression analysis were used for statistical analysis.Results Mean plasma anti-β2-GP I increased significantly in SLE group compared to control group [ (29± 19) vs (14±8) U/ml,t=2.035,P＜0.05].The plasma levels of anti-β2-GP I were higher in SLE patients with CVD than those without [(41±25)vs (18±12) U/ml,t=2.038,P＜0.05].Plasma anti-β2-GP I level was positively correlated with triglyceride (r=0.337,P＜0.05) and renal lesions (r=0.489,P＜0.01 ).Plasma anti-B2-GP [ level was negatively correlated with high density lipoproteins (r=-0.385,P＜0.05 ) and complement (r=-0.497,P＜0.05 ) level.Logistic regression analysis showed that plasma anti-β2-GP I (β=0.675,95％CI0.5070.816,P＜0.05) was an independent risk factor for CVD in SLE patients.Conclusion The level of plasma anti-β2-GP I in SLE patients with CVD is high,and it may play a role in the pathogenesis and progression of CVD in SLE patients.