Objective:To prepare curcumin liposome and investigate its stability.Methods:Curcumin liposome was prepared by the reversed-phase evaporation method and purified by exclusion chromatography with Sephadex-50.The optimum formation was selected by means of orthogonal design of experiment;Centrifugal acceleration experiment and determination of leakling ration were served to proved the liposome stability.Results:The optimum formula was as follows:the ratio of lecithin to drug was 60∶1;lecithin:cholesterol was 4∶1;pH of PBS was 6.5,ultrasonic time was 5min.The average entrapment efficiency for curcumin was 95.06%.Conclusion:The selected formulation and preparation technique of curcumin liposome is reasonable in prescription,practicable in techniques,high in encapsulation efficiency and good in stability.

A prototype of the hospital medical engineering construction and development is introduced. Functions and tasks of medical engineering are described, and its construction and development are also analyzed. Constructive opinions and views are put forward for further discussion. Detailed descriptions of the medical engineering staffing, equipment configurations are also provided. Issues such as the scientific management system and information technology in the building of archives management, technical and operational training, reward and punishment system, and intiatives of staff are focused on. The management level of medical engineering thus has been furtherly improved.

OBJECTIVE:To study the inhibitory action of Sarcandra glabra injection (ZJF) on tumor growth of SGC-7901 transplantation tumor in nude mice and its induction on apoptosis. METHODS:SGC-7901 transplantation tumor nude mice model was established then the model mice were assigned to blank,positive control,ZJF high-dose and low-dose (3,1.5 g?kg-1) groups. After treatment with ZJF for 14 days,the indexes of thymus and spleen,numbers of peripheral red blood cells,white cells and platelet were measured; the tumor-inhibition rate was computed; the ultrastructure of tumor tissues was observed,and the apoptosis of implanted tumor cells was observed. RESULTS:As compared with blank group,no significant change was noted in the indexes of thymus and spleen and peripheral hemogram. The differences between high-dose ZJF group and low-dose ZJF group were greatly significant in both the inhibition rate of SGC-7901 transplantation tumor (42.8% vs. 39.9%) and the apoptotic rate of tumor tissues [(9.3?0.15)% vs.(8.18?0.08)%].One or more apoptotic bodies formed after medication. CONCLUSION:ZJF can promote the presence of apoptotic body and induce apoptosis thus inhibiting the growth of SGC-7901 transplantation tumor in nude mice; however,it had little impact on the thymus and spleen and peripheral hemogram.

Objective To develop a medicament-induction tumor therapeutic apparatus, which is a new method for curing some kinds of tumors(liver cancer, lung cancer, intestinal tract cancer, cancer of the pancreas, etc.) Methods Based on medicament induction theory and combined with iatrology, electronics and computer technologies, microprocessor was utilized to realize data processing and treatment control. Results The medicament-induction tumor therapeutic apparatus was practical. Conclusion This apparatus is controlled by monolithic integrated circuit to induce medicament into human body through electropores and skin pores.

Objective:By adopting network pharmacology to study the mechanism of Yinchen-Erchen Decoction in treating non-alcoholic steatohepatitis (NASH). Methods:TCMSP was used to collect the chemical constituents of Yinchen-Erchen Decoction, and the effective active ingredients were obtained. Then the target of action was speculated. GeneCards database was used to collect the related genes of NASH. The visualized network of drug-target and disease-target was established by the Cytoscape software. After the analysis of key target genes, PPI network map was established to enrich GO and KEGG pathways based on key target proteins. Results:A total of 45 active ingredients and 114 drug target proteins, 463 NASH related genes and 33 drug and disease targets were obtained from the main chemical constituents of Yinchen-Erchen Decoction. The protein interaction network found that IL6, CASP3, MYC may be the key targets of Yinchen-Erchen Decoction in the treatment of NASH. GO and KEGG enrichment analysis identified 34 and 79 signal pathways related to inflammation, cell apoptosis, diabetes, NAFLD, cancer and so on. Conclusions:Yinchen-Erchen Decoction has multi-pathway and multi-target characteristics in treating NASH. It reveals the key targets, biological processes and signal pathways involved, and provide reference and basis for the following study.

Objective To develop ICU digital system for over-all monitoring and remote monitoring.Methods The programming and design was performed according to the principles of"taking patients as center,clinic as basis,utility as goal,service as aim".Results ICU digital system was composed of digital medical instruments and their clinical Gateway,intensive care information system,bedside integrated clinical information terminals,nurse workstation integrated clinical information terminals,serious patients visit system,little smart local communication system,consultation demonstration teaching system and personal digital assistant clinical information terminal system.Conclusion As one of the important symbols of modern hospital,the ICU digital system integrates the vital signs information of every patient in ICU and implement seamless connection with CIS,LIS,PACS,HIS.The construction of ICU digital system will accelerate the digital hospital's development.

The perineurial cells of the small nerve branch of the normal human abdominal wall are observed with electron microscope. The fibrous long-spacing bodies (FLS) are found within the interstitial substance of the endoneurium. 1. 2-5 layers of the perineurial cells surround the nerve fasciculus. The perineurial cells are squamous in shape and the cytoplasm contains microfilaments and pinocytotic vesicles. Each perineurial cell has an obvious basement membrane on its basal surface but the fibroblasts, whatever situated in the endoneurium or on the outer surface of the perineurium, has no basement membrane. Numerous desmosome and some gap junctions between the close attached perineurial cells are demostrated. The collagen fibrils between perineurial cells can be often shown. FLS bodies and collagen fibrils about 45 nm in diameter in the interstitial substance of endoneurium inside perineurial cells are demonstrated. In the connective tissue surrounding the outside of perineurial cells, the collagen fibrils of 80 nm in diameter can be seen, but no FLS bodies present there. 2. FLS bodies in the endoneurial matrix can be demostrated. Most of them closely associated with the basement membrane of the Schwann cells surrounding the unmyelinated nerves but no FLS bodies are found associate with those of the myelinated nerves. A few FLS bodies do not relate to basement membrane and they are invested only with the collagen fibrils. In the longitudinal sections, most of FLS bodies are in spindle shape of various sizes, their longitudinal axes parallel to that of the adjoining collagen fibrils. Sometimes the FLS bodies continue with the collagen fibrils are found. Occasionally, FLS body like a bridge locate between two Schwann cells and its dark bands continue to the basement membrane of the Schwann cells. Two or three FLS bodies may fuse together but their cross bands do not registered at the same level. In the oblique sections, FLS bodies are nearly rectangular in shape, with the cross bands shown, and their appearance do not show greaty difference from those in longitudinal sections. The periodicity of FLS bodies is about 133nm in length; the dark band in it is about 53 nm, which is made of the dense granular substance; the light band is about 80 nm, which is made of a network with approximated parallel microfilaments. There are no further crossstriated structures within the periods. Some problems, such as the role of the perineurial cells, the relationship of FLS bodies with basement membrane, are briefly discussed.

Objective:To evaluate the current situation and the developing trend of 5-HT3 receptor antagonists used in 25 hospital of Wuhan during the period of 2003～2005 in order to provide reference for clinical doctors and pharmacists. Method:The 5-HT3 receptor antagonists were statistically analyzed in respect to the purchase sum of money,DDDs and daily costs. Result:During the 3 years, purchase sum of money of 5-HT3 receptor antagonists were yearly increasing. The share of the market of domestic drugs showed an increasing tendency,while the imported drugs decreased year by year. Granisetron, ondansetron and tropisetron were much more frequently used than others. Conclusion:The market of the oral agents of 5-HT3 receptor antagonists will further expand in China.

Objective To explore the distrbution characteristics of glutathione S-transferase T1 and M1 (GSTT1 and GSTM1) gene polymorphism of Daur population in Inner Mongolia region, and provide the relevant data for studying the genotype of minority ethnic group of the Inner Mongolia.Methods Genomic DNA was extracted from peripheral blood and the fragments of GSTM1 and GSTT1 was amplified by internal control allele specific PCR in 220 volunteers who had been residents in Inner Mongolia region. At the same time, the genotype of GSTT1 and GSTM1 was resolved by the density of 2.5% agarose gel electrophoresis.Results In the studied population, the frequencies of GSTM1 (-) and GSTT1 (-) were 50.8% and 71.4% respectively. The frequency of the individuals with combination of GSTM1 (-) and GSTT1 (-) was 31.4%.Conclusion The distribution of GSTM1 and GSTT1 displays gene polymorphism in Chinese Daur population, approximate to Mongolian population in the north of China, but there is significant difference between Chinese Daur population and Han people.

OBJECTIVE:To optimize the extraction technique of tiannian capsules. METHODS:The extraction technique was selected by orthogonal method,with the contents of radix astragali and polycose,as well as the recovery of dry ointment as indicators,the influential factors for the extraction of effective ingredients that included the amount of adding water,length of decocting time and frequencies of decocting were studied.RESULTS:The optimal extraction technique is that medicinal herb is decocted in water 6 times as much as the herb for 3 times with 1 h per time.CONCLUSION:The optimized extraction technique is scientific,economic and practicable,which can be a guideline for manufacturing.