3.Chemotherapy treatment of hepatoblastoma in children
International Journal of Pediatrics 2015;42(5):553-555
Hepatoblastoma(HB) is the most common malignant hepatic tumor in the pediatric population,almost exclusively in infants and toddlers, which accounts for 50% ~ 60% of the liver tumors.Now, the comprehensive treatment program of HB in children includes surgery, chemotherapy, radiotherapy, and so on.Liver transplantation and autologous peripheral blood stem cell transplantation could also be applied to advanced or refractory hepatoblastoma in children.The high sensitivity of childhood hepatoblastoma to chemotherapeutic agents,makes chemotherapy as one of the main treatment modalities.This review mainly discusses chemotherapy treatment of HB in children.
4.Etfects of ATP on cerebral blood flow velocity and vascular responses during hyperventilation.
Chinese Journal of Anesthesiology 1994;0(04):-
The effects of ATP on cerebral blood flow velocity and vascular responses to hyperventilation using TCD in the middle cerebral artery were studied in 15 patients. Anesthesia was maintained by infusion of 1% procaine (1ml.kg-1/min) and inhalation of 1% enflurane-40%N2O-O2. Mean blood flow velocity in left middle cerebral artery was measured and cerebral vascular reactivity to hyperventilytion was assessed before and during the administration of ATP.The cerebral reactivity was expressed as the percentage change in mean blood flow velocity per unit change in PETCO2. During the administration of 1%ATP,a 34% reduction in MAP with mean blood flow velocity decreased significantly from 61.5 to 53.1cm/s (P
5.Effect and mechanism of kansui root on microcirculation of pancreatic tissues in rats with severe acute pancreatitis
Chinese Journal of General Surgery 1997;0(06):-
Objective To explore the effect and mechanism of kansui root on microcirculation of pancreatic tissues in severe acute pancreatitis(SAP) rats.Methods SD rats were randomly divided into sham group(S group),SAP group and kansui root therapy group(K group).40 rats in each group.Serum amylase,and thromboxame-B2(TXB_2),6-Keto-F_(1?)(6-Keto PGF_(1?))levels and expressions of cyclooxygenase-2(COX-2)mRNA and protein in pancreatic tissue,microscopy and election microscopy of pancreas,mortality within 72 hour after operation in each group were tested at 2h,6h,12h,and 24h after operation.Results (1)The TXB_2,6-keto PGF_(1?) levels and the ratio of TXB_2/6-Keto-PGF_(1?)(T/P) in SAP group were all(obviously) higher than those in S group(P
6.Inhibitory Action of Salidrose on Hepatic Fibrosis
China Pharmacy 2001;0(11):-
OBJECTIVE:To investigate the inhibitory action of salidrose on hepatic fibrosis of rats.METHODS:CCl 4 -induced hepatic fibrosis rat(n=55)model was built up,then were administrated low,median and high dose of salidrose positive control malotilate,and compared with normal group(n=10),the pathohistological changes were observed after6wk under light microscope and hepatic fibrosis degree was monitored before and after treatment by determining levels of ALT,AST,NO,HA and LN in serum and Hyp and MDA in liver tissue.RESULTS:Salidrose significantly reduced the elevated levels of ALT,AST,NO,HA,LN in serum and the exorbitant Hyp and MDA in liver tissue.Histopathologic examination also showed its significant amelioration effect on experimental liver fibrosis.CONCLUSION:Salidrose has inhibitory action on experimental liver fibrosis to some extent.
7.Effect of high zinc concentrations on contents of iron and zinc and expression of their regulating mRNA in Caco-2 cells
Academic Journal of Second Military Medical University 1982;0(01):-
Objective:To observe the effect of high zinc concentrations on contents of iron and zinc and expression of their regulating mRNA in Caco-2 cells.Methods: Caco-2 cells were used as model of human small intestinal enterocytes and were treated for 24 h with 4,50,100 and 200 ?mol/L of zinc.The zinc and iron contents were determined by atom absorption spectrophotography and the expression of DMT1,IREG1,ZnT1 and hZIP4 mRNA were determined by RT-PCR(the level of GAPDH was taken as internal control).Results: The zinc content in Caco-2 cells was increased 24 h after treatment with zinc,and reached the peak when exposed to 50 ?mol/L zinc.The iron content decreased with the increase of zinc concentration.Zinc supplement increased the expressions of DMT1,IREG1 and ZnT1 mRNA but decreased the expression of hZIP4 mRNA.Conclusion: It is suggested that zinc supplement can increase the contents of zinc but decrease the contents of iron in Caco-2 cells.There may be interactions between iron and zinc in the intestinal lumen.
8.Effects of 1?,25-Dihydroxyvitamin D_3 on the proliferation and apoptosis of K562 leukemia cells and its mechanism
Chinese Pharmacological Bulletin 2003;0(10):-
Aim To investigate effects of 1?,25-Dihydroxyvitamin D_3 on the growth inhibition,proliferation of K562 cells and to explore its mechanism. Methods The expression of vitamin D receptor(VDR) was indentified by indirect immunofluorescent stainings.Cell growth,proliferation,apoptosis and cells cycle were evaluated by MTT assay,acridine orange/ethidium bromide(AO/EB) and flow cytometry PI staining.The enzymatic activity of the caspases-3 class of K562 cells was determined by colorimetric assay.Results ① VDR was present in K562 cell nucleus;② 10~(-8) mol?L~(-1) 1,25(OH)_2D_3 could markedly inhibit K562 cells growth and induce cells apoptosiswith most of cells being arrested in G_2/M phase.The ratio of apoptosis increased from 4.1%(control group) to 26.5%(treatment group),P
9.Observation of clinical effects of three kinds of desensitizer on dentin hypersensitivity
Journal of Chongqing Medical University 1986;0(03):-
Objective:To eveluate the clinical effects of treating dentine hypersensitivity with three kinds of desensitizer(Prime & Bond NT,GLUMA desensitizer and 75% sodium fluoride glycerin).Methods:324 teeth with dentin hypersensitivity were divided into three groups.Group A:Prime & Bond NT adhesive.Group B:GLUMA desensitizer.Group C:sodium fluoride.Results:Instant,effective rates in group A and group B were significantly higher than that in group C(P0.05).After three months,effective rates in group A was significantly higher than that in group B(P
10.Extraction of Schisandra Chinensis (Turcz) by supercritical carbon dioxide
Chinese Traditional Patent Medicine 1992;0(08):-
AIM:To study the extraction of Schisandra Chinensis (Turcz) using supercritical carbon dioxide as extracting ageat. METHODS: The study was carried out through orthogonal test design,the content of schizandrin from Schisandra Chinensis (Turcz) was selected by 3 influencing factors, extract temperature, extract pressure and separation temperature. RESULTS:The optimal condition for the extraction as A3B2C1 Schisandra Chinensis (Turcz) was extracted by supercritical carbon dioxide, extract pressure was 25MPa, extract temperature was 50?C, separation was 7 MPa, separate temperature was 60?C, extract time was 2.5h and runing amount of CO2 was 15kg?h -1. CONCLUSION:It is feasible that supercritical carbon dioxide is used in the preparation of Schisandra Chinensis (Turcz).