Humanistic principle of education on reader of college library is an important ethics principle. Prehension of this principle is outlined. The practice of humanistic principle of education on reader of college library is recommended and the efficacy is approached. We believe in profiting all function of library in college to bring into full play and training target of college students to come true by assurance of essence of ethics and insisting on humanistic principle of education on reader of college library.

Bio-teehnology has entered all fields of TCD research and development,exerting great function in research and produc- tion of high-quality natural medicinal raw materials and sustainable utilization of Chinese herbs resource.

Objective To assess the quality of life (QoL) of rural elderly persons using the health-related dimensions of the EuroQol-5 (EQ-5D) scale to analyze the scale's reliability and validity.Methods A total of 2575 elderly residents of Tongchun rural community in Kaihua county,Zhejiang Province,were surveyed using the instrument EQ-5D,and the visual analogue scale of EQ.They included 1027 with and 1548 without chronic disease.Results The mean total EQ-5D score was 6.49 ± 1.94 for the chronic disease group and 5.15 ± 0.44 for the healthy group.The corresponding average visual analogue scale (VAS) scores were 78.15 ± 17.26 and 91.47 ± 4.37,showing significant differences.About 75％ of the elderly respondents reported no difficulties in their living.There was strong correlation between the EQ-VAS scores and the scores on all the EQ-5D scale dimensions.Conclusion The health-related quality of life reported by the rural elderly using this scale seems quite good.The EQ-5D scale,with the EQ-VAS scale as a part of it,is one useful and effective tool for evaluating the health-related quality of life of the elderly in rural areas.

The fall risk was evaluated with Modified Falls Efficacy Scale (Chinese version) in 426 elderly hypertensive patients (hypertensive group) and 436 elderly people without hypertension (control group) from rural communities in Zhejiang Quzhou areas.The fall risk scores were compared between hypertensive group and control group ; and also between patients with history of falls and those without falls.The results showed that the average score was 8.62 ± 2.15 in hypertensive group and 9.35 ± 1.35 in control group (P ＜ 0.01).The average score was 6.94 ± 1.85 in hypertensive patients with history of falls (n =112) and 9.22 ± 1.42 in without history of falls (n =314) (P ＜0.01).Our study suggests that there is a higher fall risk in elderly hypertensive patients than in elderly people without hypertension; and fall risk assessment with Modified Falls Efficacy Scale (Chinese version) can be used for prediction of fall for the elderly people in rural community.

Objective To explore the pathological changes of liver fibrosis of patients with schistosomiasis liver fibrosis.Methods Between January 2003 and January 2009,microstructure retrospective observation of liver biopsy pathological paraffin specimens by optical microscope was carried out in 9 cases of patients with schistosomiasis liver fibrosis in Quzhou people's hospital internal medicine.Then those paraffin blocks were changed to resin embedding blocks and superfine slices were made.Ultrastructure observation by electronic microscope was carried out.Results Visible fibrous scar formation and fiber calcified nodule eggs in microscopic structure of liver tissue in 9 cases of patients with schistosomiasis liver fibrosis were observed.Ultrastructural observation of the 9 cases of patients with schistosomiasis liver fibrosis showed that most of the liver cell profile was clear,liver cell nucleus gap expanded,the number of rough endoplasmic reticulum decreased,swollen mitochondria crest occurred,collagen deposited around hepatic sinus and surroundings and collagen fiber bundle around small veins extended to hepatic lobule and surroundings,and calcified nodule eggs were not easy to find.Conclusions Under an electron microscope,diseased tissue cells can be observed at subcellular level or organelle level,which provides more insight into the pathology of schistosomiasis liver fibrosis.Calcified egg nodules are easily found by an optical microscope other than an electron microscopy.The two can complement each other.

Objective To observe the abnormality of lipid metabolism and renal function change in elderly patients with hypertension,and to evaluate the relationship between atherogenic index of plasma(AIP) and glomerular filtration rate(GFR).Methods From July 2015 to June 2017,in Quzhou People's Hospital,182 elderly patients with hypertension were selected as observation group,and 192 elderly healthy people with normal blood pressure were selected as control group.The total cholesterol(TC),triglyeride(TG),high density lipoprotein cholesterol (HDL-C) were detected,and the AIP =1og [TG/HDL-C] was calculated.The GFR was estimated by Cockcroft-Gault equation.The relationship between the estimated GFR (eGFR) and the blood lipid parameters was analyzed.Results The eGFR of the observation group and control group were (84.94 ± 19.49) mL · min-1 · (1.73m2)-1,(154.91 ± 20.44) mL· min-1 · (1.73m2)-1,respectively,the difference between the two groups was statistically significant (t =11.27,P ＜ 0.01).The AIP of the observation group and control group were (0.68 ± 0.03),(-0.22 ± 0.02),respectively,the difference between the two groups was statistically significant (t =3.43,P ＜ 0.01).There was a negative correlation between AIP and eGFR in the elderly patients with hypertension (r =-0.845,P ＜ 0.01).Conclusion The fact shows that abnormality of lipid metabolism in the elderly patients with hypertension is present.AIP can be used as a plasma marker of atherosclerosis and could be used as a useful and important parameter for clinical observation in the elderly hypertensive patients with renal impairment.

Objective To investigate the correlations of extracellular matrix and hepatic ultramicrostructural changes with clinical manifestations in patients with mild chronic hepatitis B (CHB).Methods Patients with chronic HBV infections were enrolled and were divided into mild CHB group (n=66) and HBV carrier group (n=10).Serum samples were collected from patients, and serum HBV markers, HBV DNA load and liver fibrosis indexes were measured.All subjects received liver biopsy, and the tissue samples were observed by light microscope and electron microscope.T test and χ2 test were performed for measurement data and enumeration data, respectively.Spearman test was used for ranked data.Results The differences on ALT and AST levels between mild CHB group and HBV carrier group were significant (t=12.42, 7.06, P＜0.05), but there was no significant difference on HBV DNA load between two groups (t=0.24, P ＞ 0.05).Serum liver fibrosis indexes (hyaluronic acid, type Ⅲ collagen,type Ⅳ collagen and laminin protein) in mild CHB group were not significantly higher than those in HBV carrier group (t=0.45, 0.95, 0.76 and 1.21, P ＞0.05).In mild CHB group, there were 33 patients with ≥G2 and ≥S2, but in HBV carrier group were only 2 patients (χ2=4.17, P ＜ 0.05).Seventeen patients in mild CHB group were with S3-4, while that was not observed in HBV carrier group (χ2=4.75, P ＜0.05).In mild CHB group, hepatic ultramicrostrutural changes on fat storing cell, collagen protein and portal area were correlated with fibrosis grades, and the correlation coefficients were 0.351, 0.675 and 0.301, respectively (P=0.004, 0.000 and 0.014).Conclusion Electron microscope is of higher sensitivity than light microscope in observing hepatic ultramicrostructural changes, which is effective in evaluating the severity of mild CHB.

Objective To identify the risk factors of Burkholderia cenocepacia associated nosocomial lower respiratory tract infections (NLRTIs),and to investigate the drug resistance of Burkholderia cenocepacia strains.Methods A total of 138 patients with Burkholderia cenocepacia associated NLRTIs and 40 patients with non-Burkholderia cenocepacia associated NLRTIs were enrolled in the study.All patients were collected from the Second Affiliated Hospital of Wenzhou Medical University during January 2009 and December 2012.Clinical data and results of drug sensitivity tests were retrospectively reviewed.Chi-square test and Logistic regression analysis were performed to identify the risk factors of Burkholderia cenocepacia associated NLRTIs.Results Logistic regression analvsis showed that combination use of 2 or more antimicrobial agents,mechanical ventilation,stay in intensive care unit (ICU) for more than two weeks,use of antacid H2 antagonist and deep venous puncture were the independent risk factors of Burkholderia cenocepacia associated NLRTIs (OR =6.315,5.957,5.254,4.585 and 2.017,P ＜0.05).Burkholderia cenocepacia strains were sensitive to levofloxacin,ceftazidime and sulfamethoxazole; More than 40％ strains were resistant to cefotaxime,ceftriaxone,cefepime,aztreonam and tetracycline; And nearly 100％ strains were resistant to gentamicin,amikacin and tobramycin.Conclusion Burkholderia cenocepacia associated NLRTIs are more likely to occur in patients with combination use of 2 or more antimicrobial agents,mechanical ventilation,and those who stay in ICU for more than two weeks,or received antacid and deep venous punctures,and most Burkholderia cenocepacia strains are multiple drug resistant.

Objective To study the protein oxidative damage and its possible mechanism caused by multi-walled nanotubes(MWCNTS)in mice.Methods Totally 20 Kun-ming mice were divided randomly into 4 testing groups(n=5 for each group),with 0.1,0.2 and 0.4mg/ml MWCNTS suspension injected groups and saline injected group as control group.After 5 days exposure,the protein carbonyl content was measured by using spectrophotometric DNPH assay to reflect the degree of protein oxidative damage.Results The results showed that the protein carbonyl content in livers in 0.2 and 0.4mg/ml MWCNTS suspension injected groups were significantly higher than that in saline group(P

Objective To explore the effects of different treatments on prognosis of patients with Gynura segetum caused hepatic vein occlusion disease (HVOD).Methods From July 2008 to January 2016,85 patients with Gynura segetum caused HVOD were enrolled and received treatment of liver function protection and microcirculation improvement.According to different treatment options,patients were divided into non-anticoagulation group,nowanticoagulation transfered to transjugular intrahepatic portosystem stent-shunt (TIPS) group,anticoagulation group,anticoagulation transfered to TIPS group and anticoagulation-TIPS step-by-step treatment group.The efficacy of each group was observed.Chi square test was performed for statistical analysis.Results Among 22 patients who received nonanticoagulation treatment,six (27.3％) patients were cured and 14 (63.6％) patients died during the treatment period;besides two (9.1 ％) patients received TIPS because of ineffective treatment and achieved longterm survival.Among 63 patients treated with combination of low-molecular-weight heparin and warfarin,six (9.5％) patients died and 36(57.1％) patients were cured.The cure rate was higher than that of nonanticoagulation group (x2 =5.820,P=0.016).Other 21 patients received TIPS treatment,achieved longterm survival except one patient died from surgical complications.The cure rate of anticoagulation-TIPS step treatment group was 88.9 ％ (56/63) which was higher than that of non-anticoagulation group,and the difference was statistically significant (x2 =31.350,P＜0.01).Conclusions Compared to conventional liver function protection treatment and symptomatic treatment,anticoagulation therapy significantly increases the cure rate of patients with Gynura segetum caused HVOD.Anticoagulation-TIPS step-by-step treatment further improves the cure rate.