Objective: To establish the quality standard for Ganmaoyangkeling Tablets(Radix Isatidis, Herba Solidaginis, Radix Glycyrrhizae, Rhizoma Zingiberis Recens, etc.). Methods: Radix Glycyrrhizae and Herba Solidaginis were identified by TLC, and the content of quercetinum was determined by HPLC. Results: Quercetinum shows a good linear relationship in the range of 0.05～0.40?g ( r =0.99997), and the average recovery is 101.9%, RSD is 2.07%. Conclusion: These methods is simple, accurate and specific and can be used for the quality control of Ganmaoyangkeling Tablets.

It is the requirement to change the utility reading of medical undergraduates and to realize the functions of medical academic library for medical academic library to popularize reading service .The serial reading populari-zation service practice was thus described in this paper with certain feasible suggestions put forward for the further reading popularization activities.

Attention Deficit Disorder with Hyperactivity ; diagnosis ; therapy ; Humans

Attention Deficit Disorder with Hyperactivity ; epidemiology ; Child ; Comorbidity ; Humans

Objective To measure the serum levels of angiopoietin-like protein (ANGPTL) 3, ANGPTL4 and RANKL in patients with rheumatoid arthritis (RA), and the relationship between ANGPTL3/ANGPTL4 and clinical manifestations were analyzed. Methods Blood samples were drawn from 72 patients with RA and 28 healthy subjects. Serum levels of ANGPTL3, ANGPTL4 and RANKL were tested by enzyme linked immuno-sorbent assay (ELISA). Nonparametric rank sum test was used for the comparisons between groups and Spearmanˊs correlation test was used for correlation analysis. Results ① The serum level of ANGPTL3 was significantly increased in RA group compared to healthy group [800.325(577.477, 1 750.636) pg/ml vs 487.900 (382.340, 565.499) pg/ml, P<0.05, Z=-6.082]. ② The serum level of ANGPTL4 was significantly increased in RA group compared to healthy group [1 036.199(853.347, 1 746.677) pg/ml vs 706.095(558.571, 807.302) pg/ml, Z=-5.962, P<0.05].③ The serum levels of ANGPTL3 and ANGPTL4 in RA patients were increased with the rise of disease activity (DAS 28) [ANGPTL3 in the low disease activity groupwas 457.265 (373.709, 605.296) pg/ml, that of the moderate disease activity group was 785.815(679.156, 1 308.785) pg/ml, that of the high disease activity group was 1 502.038 (817.713, 1 960.493) pg/ml, P<0.05; ANGPTL4 in the low disease activity group was 737.604 (467.040, 918.222) pg/ml, that of the moderate disease activity group was 991.227 (819.456, 1 699.972) pg/ml, and that of the high disease activity group was 1 842.310 (1 252.023, 2 669.902) pg/ml, P<0.05].④ANGPTL3 and ANGPTL4 was positively correlated with RANKL (r=0.554, 0.619, P<0.01).⑤ The serum level of ANGPTL4 was significantly increased in patients with bone destruction, compared to those without bone destruction [1 624.071(949.432, 2 622.371) pg/ml vs 927.590(737.604, 1 409.798) pg/ml, Z=-2.483, P<0.05]. Conclusion The serum levels of ANGPTL3 and ANGPTL4 are increased in RA, and are both positively correlated with disease activity, moreover, ANGPTL4 is associated with pathological bone destruction.

This study revealed that the content of intra and extracellular polysaccharides may be related to the cultural conditions.With decreasing NaHCO 3 or NaNO 3 concentration,the content of intracellular polysaccharide was reduced,Reduction of NaNO 3 concentration still caused the rise of extracellular polysaccharides.The K 2HPO 4 or NaCl concentration also had an effect on the production of both intracellular and extracellular polysaccharides.When PH value of media maintained at 8.0,there was the highest content of intra and extracellular polysaccharides.The same high content also occurred after culture of nine days.In addition the content of intracellular polysaccharide was higher under continuous light than under the light/dark circle.

Objective To investigate the characteristics of mesenteric vascular thrombosis (MVT) in patients with antiphospholipid antibody syndrome (APS).Methods The cases reports about MVT in patients with APS were searched in Pubmed and Chinese biomedical database (1983.1-2007.7) and then were analyzed.Results There were 13 males and 8 females in 21 patients.The average age was (37±17) years (5months～69 years).Three cases (14%) had a history of deep venous thrombosis and 4 (19%) had spontaneous abortions.The course of disease was 4 hours to 4 months.The clinical manifestations included abdominal pain 18 (86%),hemafecia or melaena 4 (19%),vomiting 3 (14%),diarrhea 2 (10%),hematemesis 2(10%).Physical signs included abdominal tenderness in 10(48%),peritoneal irritation signs in 5 (24%),shifting dullness in 3 (14%) anddecreased bowel sounds in 3 (14%).Mesenteric vascular thrombosis were detected through B uhrasonography (3/10,33%),abdominal CT (9/13,69%),MRI (4/4,100%),Doppler ultrasound (4/4,100%),angiography (6/6,100%).Eighteen cases (86%) had positive anti-cardiophospholipin antibody and 14 (67%) were IgG-subtype.Lupus anticoagulants were detected in 4 (19%).Sixteen cases reee-ived exploratory laparotomy,lsehemia or necrosis of intestine were found in 9(56%).In 21 cases,superior mesenteric vein thrombosis,suprior mesenteric artery thrombosis,inferior mesenteric vein thrombosis,inferior mesenteric artery thrombosis were discovered in 17 (81%),4 (19%),0 (0%),1 (5%)patients respectively.Portal vein was also involved in 7 (33%) cases who had superior mesenteric vein thrombosis.Conclusion Superior mesenteric vein is usually involved in patients with APS who have MVT.MVTs are always occurr in middle-age male patients.Some patients have deep vein thrombosis or spontaneous abortion before MVT.The disease may be fulminant or had is insidious in onset.Abdominal pain and intestinal obstruction are the most common manifestations.IgG-subtype anticardiophospholipin antibodiesare the common antibodies discovered in these patients.Combined more than one image methods can detect the thrombi effectively.Anticoagulation and essential exploratory laparotomy are effective interventions in most cases.

Objective To investigate the effect of mycophenolic acid (MPA) on the endotheline-1 (ET-1) induced proliferation, contraction and migration of pulmonary arterial smooth muscle cells (PASMCs)and to explore the mechanism of MPA on pulmonary arterial hypertension (PAH), and the effect of exogenous guanosine nucleotide reversing anti-proliferative effect of MPA. Paired-samples t-test was used for statistical analysis. Methods MTT test, scarification test, Millicell cell culture insertion and the length of PASMCs mcasured under microscope were used. Results The A values of group ET-1 + low concentration MPA decreased when compared with group ET-1 (0.348±0.036 vs 0.447±0.013, t=6.357, P=0.000) and the A values of group ET-1 + high concentration MPA was further decreased. The A values of group ET-1 + low concentration MPA + guanosine was higher than that of group ET-1 + low concentration MPA (0.390±0.018 vs 0.348 ±0.036, t=2.573, P=0.028). The average migration distance and the average migration numbers of PASMCs of groups MPA was decreased than that of group ET-1. The average cell length of PASMCs of groups MPA was increas ed than that of group ET-1. Conclusion MPA can effectively inhibit the proliferation,contraction and migration of PASMCs by ET-1 induction. The IMPDH may play a role in anti-proliferative effect of MPA on PASMCs, but is unnecessary to be the sole mechanism. These findings has provide new insight into the mechanisms of mycophenolate mofetil in the treatment of PAH.

As a species of acute respiratory tract pathogen in mankind, Chlamydia pneumoniae can cause upper respiratory tract infection, pneumonia, it may also play an important role in bronchial asthma, chronic obstructive pulmonary diseases, sarcoid, lung cancer.

Objective To investigate the effect of mycophenolic acid (MPA) on the proliferation of rat pulmonary microvascular endothelial cells (PMVECs) and the effects of MPA on the secretion of endothelin-1 (ET-1) and interleukin-6 (IL-6).Methods PMVECs were cultured by tissue-sticking method.Cells were divided into 5 groups,named as the control group,low-dose MPA group,moderate-dose MPA group,highdose MPA group and super high-dose MPA group,according to the final concentration of MPA (0,0.1,1,10 and 100 μmol/L,respectively).The proliferation of rat PMVECs was detected by cell counting kit-8 (CCK-8).The ET-1 and IL-6 secretion were assessed by radio-immunoassay.One-way analysis of variance was used to detect the differences between the group means.Results The cultured PMVECs of rats in vitro showed fusiform or polygonal in shape,and the expressed factor Ⅷ associated antigen stain was positive.At 24 h,the A values of the 5 groups (from control group to super high-dose MPA group) were 1.15±0.20,1.20±0.13,0.88±0.20,0.62±0.05 and 0.56±0.10,respectively,there was no significant difference in the A values on 450 nm between the control group and the low-dose MPA group (P=0.388),as well as the high-dose MPA group and super high-dose MPA group (P=0.292),but there were significant differences between any other two groups (P＜0.01).At 48 h,the A values of the 5 groups (from control group to super high-dose MPA group)were 2.61±0.21,2.52±0.23,1.99±0.17,0.84±0.16 and 0.65±0.12,respectively,there was no significant difference in the A values on 450 nm between the control group and low-dose MPA group (P=0.094),but there were significant differences in the A values between any other two groups (P＜0.01).At 72 h,the A values of the 5 groups (from control group to super high-dose MPA group) were 2.62±0.24,2.62±0.19,2.37± 0.38,0.79±0.17 and 0.61±0.17,respectively,there was no significant difference in the A values on 450 nm between the control group and low-dose MPA group (P=0.931),but there were significant differences in the A values between any other two groups (P＜0.05).There were no significant differences in the ET-1 concentrations between the control group and low-dose MPA group (P=0.156),and high-dose MPA group and super high-dose MPA group (P=0.262),but there were significant differences in the concentrations between any other two groups (P＜0.01).There were no significant differences in the IL-6 concentrations of the five groups between any of the two groups (P＞0.05).Conclusion MPA can inhibit fetal bovine serum induced rat PMVECs proliferation and the secretion of ET-1 in a dose-dependent manner.