Objective To study the technique of using the postauricular revolving door island flap in one-stage reconstruction of concha defect and its efficacy.Methods Fifteen cases of concha de fect were repaired by the postauricular revolving door island flap in one stage reconstruction from 2000-2010.The maximum size of defect was 2.7 cm× 3.0 cm,and the minimum was 1.0 cm×1.3 cm.Results All the island flaps were survived postoperatively,and satisfactory in auricular appearances of all patients by 6 months to 5 years follow-up.Conclusions The revolving door island flaps with abundant blood supply are transferred to reach the auricular defect,which match normal auricular tissue in contour,color,and texture. This flap on the retroauricular area is relatively concealed,and it therefore can be one of the best procedures for repair of the auricular defects,especially concha.

Complex regional pain syndrome (CRPS) is a neuropathic pain disorder, characterized by persistent and severe pain after trauma or surgery. Diagnosis is mainly based on clinical signs and treatment is based on a multidisciplinary approaches, including non-pharmacological approaches and occupational therapy，pharmacotherapy and invasive therapeutics. This article reviewed the current advice of diagnosis and treatment of CRPS.

Attention Deficit Disorder with Hyperactivity ; diagnosis ; therapy ; Humans

China ; Fracture Fixation, Internal ; education ; Humans ; Maxillofacial Injuries ; therapy ; Societies, Medical ; Surgery, Oral ; education

Attention Deficit Disorder with Hyperactivity ; epidemiology ; Child ; Comorbidity ; Humans

China ; Facial Injuries ; surgery ; Humans ; Mouth ; injuries ; Traumatology

Objective:To establish a laser light scattering method for the determination of the particle size distribution of clostridium butyricum enterococcus triple viable powder and compare the results of the sieving method.Methods:The conditions of laser scattering method were as follows:the vibration sampling rate of 80%,the dispersion pressure of 0.05 MPa,the background and sample scan time of 15 s,the shading of 0.5%-5%,the refractive index of the particles of 1.55,the particle absorption rate of 0.01,and the injection volume of 0.1-0.2 g.The eigenvalues of the particle size distribution were determined,which were the particle size cumulative distribution map of 10%,50% and 90% of the particle size value and the volume average particle diameter D.Results:The RSDs of d(0.1),d(0.5) and d(0.9) were less than 5% in the methodology study.The results of laser light scattering method showed that the particle size of 93.3% samples was below 250 μm,that of 64.2% samples was below 150 μm,that of 51.4% samples was below 125 μm,and that of 31.3% samples was below 90 μm.The results of sieving method showed that the particle size of 96.6% was below 250 μm,that of 46.4% samples was below 150 μm,that of 23.5% samples was below 125 μm,and that of 1.4% samples was below 90 μm.Conclusion:Sieving method and laser light scattering method both can characterize the particle size distribution of the sample.The laser light scattering method is simple,accurate and producible,which is suitable for the particle size control of clostridium butyricum enterococcus triple viable powder.

For tic disorders(TD),non -drug therapy including psychotherapy,behavioral intervention,neural regulation have demonstrated efficacy in reducing tic severity,managing co -occurring psychiatric symptoms and impro-ving life quality in addition to pharmacotherapy.Psychotherapy such as supportive psychotherapy,family intervention, school intervention can be major contributors to the development of evidence -based non -drug treatments.Different behavioural therapies that were used included positive reinforcement,extinction,massed negative practice,relaxation therapy,habit reversal training(HRT),exposure with response prevention,self -monitoring,contingency management, cognitive -behavioural therapy,assertiveness training,tension -reduction technique.Overall,HRT is the best -studied and most widely -used technique and there is sufficient experimental evidence to suggest that it is an effective treat-ment.Evidence suggests that exposure with response prevention and self -monitoring are effective,but comprehensive behavioral intervention for tics (CBIT)show more effective and durability of benefit over time.CBIT may promote nor-malization of aberrant cortico -striato -thalamo -cortical associative and motor pathways in individuals with tourette′s syndrome.Based on available evidence,recent published guidelines suggest that CBIT can be considered a first -line treatment for persons with TD.Neural regulation included electroencephalogram biofeedback,deep brain stimulation, transcranial magnetic stimulation are increasingly considered when tics become troublesome or even disabling or self -injurious despite optimal medical therapy.

Aim To investigate effects of 1?,25-Dihydroxyvitamin D_3 on the growth inhibition,proliferation of K562 cells and to explore its mechanism. Methods The expression of vitamin D receptor(VDR) was indentified by indirect immunofluorescent stainings.Cell growth,proliferation,apoptosis and cells cycle were evaluated by MTT assay,acridine orange/ethidium bromide(AO/EB) and flow cytometry PI staining.The enzymatic activity of the caspases-3 class of K562 cells was determined by colorimetric assay.Results ① VDR was present in K562 cell nucleus;② 10~(-8) mol?L~(-1) 1,25(OH)_2D_3 could markedly inhibit K562 cells growth and induce cells apoptosiswith most of cells being arrested in G_2/M phase.The ratio of apoptosis increased from 4.1%(control group) to 26.5%(treatment group),P

Objective To investigate the protein change in immature dendritic cells(DC) exposed to sendai virus replication sequence.Methods The total cellular proteins expressed in DC infected with Sev/△F or SeV were collected and quantitated,then separated by two-dimensional gel electrophoresis and visualized by silver staining.Digital images were analyzed by PDQuest software.The differentially expressed protein spots were picked,digested in gel and subjected to peptide mass fingerprinting using MALDI-TOF MS.Results Protein spots in 2-DE gel for DC treated by Sev/△F were more than that by SeV,among which a lot of proteins increased.Conclusion It was relevant to the replication sequence that proteins decreased in dendritic cells infected with sendai virus vector.