OBJECTIVE:To establish a HPLC method for content determination of ginsenoside Rg1 in Fufangtianqi dropping pills.METHODS:The separation was performed on a Shim-pack VP-ODS column at the 25℃,the mobile phase was composed of a mixture of acetonitrile-water(25∶75),the flow rate was 1.0 ml/min,the ultraviolet wavelength was 210nm,the samplesize was 20?l.RESULTS:The linear range of gindenoside Rg1 was 37.2～186.0?g/ml(r=0.9 999),and the average recovery was 101.01%(RSD=0.90%,n=5).CONCLUSIONS:This method is simple,sensitive and accurate,and can be available for the quality control of Fufangtianqi dropping pills.

Objective To analyze the change in immune function and therapeutic effectiveness of children with im-mune thrombocytopenic purpura and Helicobacter pylori(H. pylori)infection. Methods Eighty hospitalized chil-dren with thrombocytopenic purpura between March 2011 and March 2012 were as observation group,and then sub-divided into infected group and non-infected group according to whether they were infected with H. pylori;80 healthy children were as control group . Lymphocyte subsets ,clinical therapeutic effectiveness and recurrence rate among three groups were compared. Results The positive rate of H. pylori in observation group was significantly higher than control group(55.00% [44/80]vs 2.50% [2/80];χ2= 12.48,P= 0.008). There was significant differences in CD4+,CD4+/CD8+ ,and CD19+T lymphocyte among three groups (all P<0.05);CD4+ and CD4+/CD8+ T lymphocyte in infected group was significantly lower than non-infected group respectively ([13.40±4.65]% )vs [28.56± 3.82]% ;[0.69±0.18]% vs [1.04±0.23]% ),and CD19+T lymphocyte in infected group was significantly higher than non-infected group ([45.21±10.20]% vs [22.05±2.23]% );CD4+ and CD4+/CD8+T lymphocyte in non-in-fected group and infected group was significantly lower than control group ([40.20±3.42]% ,[1.54±0.42]% respec-tively),and CD19+T lymphocyte was significantly higher than control group ([11.02±2.89]% ). The overall therapeu-tic effectiveness in infected group and non-infected group was 90.91% and 91.67% respectively(P>0.05). The recur-rence rate of thrombocytopenic purpura after anti-H.pylori infection therapy in infected group was significantly lower than non-infected group (20.45% vs 30.56% ;χ2= 6.396,P= 0.038). Conclusion Detection of immune function is helpful for clinical diagnosis of H. pylori infection associated with immune thrombocytopenic purpura .

Objective To establish a HPLC method for the content determination of berberine hydrochloride in Xiaozhi Granule. Methods HPLC was performed on ODS column, the mobile phase consisting of acetonitrile-0.5%acetic acid (20∶80)and detection wavelength at 346 nm. Results The linearity of berberine hydrochloride was good in the range of 3.84～38.4?g/mL (r=0.9998), and the average recovery was 100.52 %, RSD=l.08 %(n=5). Conclusion This method was simple, sensitive and accurate , and can be used for the quality control of Xiaozhi Granule.

Objective To establish a method for the content determination of shikonin in Radix Arnebiae Oil by HPLC.Methods The content of shikonin was determined by HPLC and the chromatographic conditions were: Shim-pack VP-ODS column(4.6 mm?250 mm),the mobile phase consisting of methanol-0.025 mol/L H3PO4(85 ∶15),detection wavelength being 516 nm,tempeature at 25 ℃and the flow rate being 20 ?L.Results A good linearity of shikonin was in the range of 11.2 ?g/mL～33.6 ?g/mL(r=0.9998),and the average recovery rate was 101.03 %,RSD=1.91%.Conclusion This method was simple,accurate and with a good reproducibility and can be used for the quality control of Radix Amebiae Oil.

Head and neck surgery is a relatively young discipline. Since 1937,it has made important advances. In the past, the patients had to sacrifice their involved organs in order to survive, but now we have to think not only of the survival rate of the patients,but also should pay more and more attention to improve their quality of life. The plan of treatmant must consider multimodality therapy and function preservation. To attain this goal, we should pay attention to laparoscopic surgery and sentinal node biopsy as well as possibility of gene therapy in head and neck tumors. In addition, we should investigate to application of molecular biology in head and neck surgery.

Consensus ; Europe ; Humans ; Internationality ; Pulmonary Medicine ; Societies, Medical ; United States

Deglutition Disorders ; Humans ; Otorhinolaryngologic Diseases

Objective To discuss the indications and efficacy for treating tibia insertion avulsion fracture of the posterior cruciate ligament(PCL)by the mean of the end of ligament suture via arthroscopy.Methods Twenty-one cases of tibia insertion avulsion fracture of PCL were treated with suture the end of ligament via arthroscopy from June 2007 to June 2009,including 14 males,7 females,with a mean age of 31.5 years(range,13-52 years).The insertion avulsion fragment was fixed to the tibia with two strands of 5Ethibond threads via arthroscopy.Two bone tunnels(2.5 mm in width)were made from the anterior part of the tibia to the posteromedial(at 4:30 toward)and posterolateral(at 7:30 toward)of the tibia bone bed respectively.Then the sutures were pulled out through the two bone tunnels and tied to the door-nail.Reduction and healing of the avulsion fragment,relaxation and mobility degree of knee,and the influence of osteoepiphysis to teenage patients were observed after surgery.KT-2000 value and Lysholm score were calculated.Results All cases were followed up from 10 to 24 months,with an average of 13.5 months.All the fragments were united without displacement in all cases according to X-ray films.One case showed posterior drawer test(+),but the tibia insertion was healed.The knee extension in all cases was unlimited,and 10° to 15° flexion limitation was found in 2 cases.The average range of flexion was 140.5°±3.8°.On average,KT-2000 value was(1.2±0.4)mm,Lysholm score was 95.2±2.7.Conclusion For treating the avulsion fracture of PCL,suture the end of ligament via arthroscopic transfixion can get a reliable fixation and a satisfied function of the knee,especially,which is suitable for the small avulsion fracture fragments,the adolescent patients with premature epiphysis.

ObjectiveThis paper aims to make a research over clinical meaning of VMC with PVB and HRV.Methods22 kids who suffer from VMC with PVB and 28 kids who suffer from HRV and to compare and research their Heart Rate Variability within 24 hours.ResultsThe HRV of the VMC with PVB sufferers had decreased apparently within 24 hours( with discrepancy P ＜0.01 ).ConclusionThere exists apparent DIND in the VMC with PVB sufferers.HRV could be used to check the severness of the disease and prognosis.

Purpose：To determine the treatments and prognostic predictors of malignant melanoma of the head and neck. Methods：Surgical treatment and outcome was analyzed for 28 patients with malignant melanoma of the head and neck at department of the head and neck of our hospital (1984-1994). Results：23 patients underwent local excised as the first treatment, and local recurrence was demonstrated in 8 (34.8%). Margin of resection of primary melanoma was divided into narrow excision (≤2 cm) and wide excision (＞2 cm), and 5-year survival was 58% and 40%(P＜0.01). According to surgical methods, 5-year survival of WE, WE/END and WE/TLND were 80%, 80% and 39%. Conclusions：An excision with 2 cm margin is safe procedure for primary tumor of malignant melanoma of the head and neck. Neck dissection may not be practiced until neck lymph node is positive. Neck dissections differ from the positions of the primary tumors, and the flaps must be raised preferably superficial to the platysma.