0.05).We conclude that the urine ?—HCG ELISA has the same value as RIA in the early pregnancy diagnosis. It may be used as a routine method, because it is without radiactivity, and simpler, cheaper, more rapid and practical than RIA, especially it is easy to be generalized in the country.

Objective:To discuss the influence of rosuvastatin intensive lipid-lowering on oxidative stress in the patients with un-stable angina pectoris ( UAP) . Methods:Totally 92 cases of patients with UAP were divided into the observation group and the control group at random with 46 cases in each. The patients in the two groups were given aspirin,β-blocker, nitrate preparations, angiotensin converting enzyme inhibitor ( ACEI) and the other basic medical treatment, and the patients in the control group were additionally giv-en 10mg rosuvastatin, qd, before sleeping, while the patients in the observation group were additionally given 20mg rosuvastatin, qd, before sleeping, and the course of treatment was 8 weeks. The changes of serum SOD, MDA and LPO levels in the two groups before and after the treatment were observed, and the adverse drug reactions ( ADR) were compared as well. Results: The rate of follow-up loss of the two groups had no statistically significant difference (P>0. 05). After the 8-week treatment, the serum MDA and LPO lev-els of the patients were obviously declined, while the SOD levels were obviously increased in the two groups (P<0. 05 or P<0. 01), and the changes in the observation group were more notable than those in the control group (P<0. 05). The incidence of ADR in the two groups had no statistically significant difference (P>0. 05). Conclusion:Rosuvastatin intensive lipid-lowering has high security. The mechanism may closely related to the effects of reducing serum MDA and LPO levels, increasing SOD levels, inhibiting lipid per-oxidation and enhancing antioxidant capacity.

To clone the murine alpha-fetoprotein (AFP) gene, construct the eukaryotic expression vector of AFP and express in CHO cells, total RNA were extracted from Hepa 1-6 cells, and then the murine alpha-fetoprotein gene was amplified by RT-PCR and cloned into the eukaryotic expression vector pcDNA3.1. The recombinant of vector was identified by restriction enzyme analysis and sequencing. After transient transfection of CHO cells with the vector, Western blotting was used to detect the expression of AFP. It is concluded that the 1.8 kb murine alpha-fetoprotein gene was successfully cloned and its eukaryotic expression vector was successfully constructed.
CHO Cells ; Cloning, Molecular ; Cricetinae ; DNA, Complementary ; Eukaryotic Cells/metabolism ; Genetic Vectors ; Reverse Transcriptase Polymerase Chain Reaction ; Transfection ; alpha-Fetoproteins/*biosynthesis ; alpha-Fetoproteins/genetics

Adult ; Female ; Humans ; Intestinal Obstruction ; etiology ; therapy ; Postoperative Complications ; etiology ; therapy ; Rectal Neoplasms ; radiotherapy ; surgery

Objective To evaluate the efficacy of ultrasound-guided transversus abdominis plane (TAP) block for postoperative analgesia in patients undergoing inguinal hernia repair.Methods Forty ASA Ⅰ or Ⅱ patients,aged 18-79 yr,with body mass index ＜ 30 kg/m2,scheduled for elective unilateral inguinal hernia repair combined spinal-epidural anesthesia,were randomly divided into 2 groups (n =20 each):control group (group C) and ultrasound-guided TAP block group (group B).The ultrasound-guided TAP block was performed at the end of surgery and 20 ml of 0.375 ％ ropivacaine was injected in group B,while the equal volume of normal saline was given in group C.Tramadol was injected intravenously when VAS score ≥ 4 after surgery.VAS scores at rest and during activity were recorded at 4,6,24 and 48 h after surgery.The warm block plane on the blocked side was measured at 24 and 48 h after surgery.The overall satisfaction on analgesia was scored and the time when the patients passed the flatus was recorded.TAP block-related side effects were recorded.Results Four patients required tramadol in group C,while no patients required rescue analgesic in group B.Compared with group C,VAS scores were significantly decreased,the overall satisfaction scores were increased (P ＜ 0.05 or 0.01),and no significant change was found in the time when the patients passed the flatus in group B (P ＞ 0.05).The rate of warm plane block on the blocked side was 80％ at 24 h after surgery and there was not warm block plane in patients at 48 h after surgery in group B.There was not warm block plane in patients at 24 and 48 h after surgery in group C.TAP block-related side effects were not found in group B.Conclusion The efficacy of ultrasound-guided TAP block for postoperative analgesia is better in patients undergoing inguinal hernia repair and the safety is higher.

Objective To investigate the expression of ER and PR in hyperplasia of mammary glands and breast cancer. Methods The expression of ER and PR in 68 hyperplasia of mammary glands cases and 168 breast cancer cases were quantified by immunohistochemical method. Results The expression of ER in breast cancer(30%) was significantly higher than that in hyperplasia of mammary glands(10 %)(P ＜0.05) and there was no significant difference in PR between them. The expression of ER in postmenopausal breast cancer was significantly higher than that in postmenopausal hyperplasia of mammary glands (P ＜0.05) and there was no significant difference in ER between two premenopausal groups. The expression of PR in invasive lobular carcinoma was significantly higher than that in invasive ductal carcinoma and other types (P=0.005).Conclusion The expression of ER and PR may identify the characteristics of patholobiology in breast disease.

Objective To evaluate the effect of captopril and isoflurane preconditioning on cell apoptosis during myocardial ischemia/reperfusion (I/R) in rabbits.Methods Forty New Zealand white rabbits of both sexes,weighing 1.8-2.5 kg,were randomly divided into 5 groups (n =8 each) using a random number table:sham operation group (group S),group I/R,isoflurane preconditioning group (group I),captopril preconditioning group (group C) and captopril and isoflurane preconditioning group (group C + I).The animals were anesthetized with 3％ pentobarbital sodium 30 mg/kg.Myocardial ischemia was induced by occlusion of the left anterior descending branch of coronary artery for 30 min followed by 120 min of reperfusion.1.1％ isoflurane was inhaled for 30 min followed by 15 min washout before myocardial ischemia in group I.Captopril 25 mg/kg was given through a gastric tube into the stomach at 24 h before myocardial ischemia in group C.Captopril 25 mg/kg was given through a gastric tube into the stomach,24 h later 1.1％ isoflurane was inhaled for 30 min followed by 15 min washout,and then myocardial ischemia was performed in group C + I.The rabbits were sacrificed at the end of reperfusion and myocardial specimens were removed for microscopic examination and observation of ultrastructure,and for determination of the expression of Bcl-2 and Bax proteins (by Western blot).The apoptosis rate was detected by flow cytometry.Bcl-2/Bax ratio was calculated.Results Compared with group S,the apoptosis rate was significantly increased,the expression of Bcl-2 and Bax proteins was up-regulated,and Bel-2/Bax ratio was decreased in I/R,I,C and C + I groups (P ＜ 0.05).Compared with I/R,I and C groups,the apoptosis rate was significantly decreased,Bcl-2 protein expression was up-regulated,Bax protein expression was down-regulated,and Bcl-2/Bax ratio was increased (P ＜ 0.05),and the pathological changes were significantly attenuated in group C + I.Conclusion Regulation of Bcl-2/Bax ratio and inhibition of apoptosis in myocardial cells are involved in the mechanism by which isoflurane and captopril preconditioning reduces I/R injury in rabbits.

r cirrhosis. NO and ET-1 are involved corporately in the development of liver cirrhosis, but not the main factors facilitating hypoxemia of liver cirrhosis.

Objective To investigate the effects of 1,25(OH)_2D_3 on cell proliferation and cell cycle progression in mouse osteoblasts.Methods Sterile bones of skull of mouse were taken from 30 newborn mouse,and the osteoblast were separated by enzyme digestion methods.After 1,25(OH)_2D_3 in different concentrations were added into culture medium,the effects of 1,25(OH)_2D_3 on cell proliferation of mouse osteoblasts and on cell cycle progression were examined by mono-nuclear celldirect cytotoxicity assay(MTT)reduction assay and flow cytometry respectively.Results After 24,48,72 h of 1,25(OH)_2D_3 incubation,the cell number of osteoblast had significant difference among groups of 1,25(OH)_2D_3 of 10~(-8),10~(-9),10~(-11)mol/L.Significant differences were found in the cell cycle progression in response to 1,25(OH)_2D_3 treatment from the Gl(84.30?1.90)to the G2-M (7.70?0.667)and S(8.00?1.42)phases when compared with those in the control group. Conclusions Cell proliferation of mouse osteoblasts can be inhibited by 1,25(OH)_2D_3 in a concentration-dependent manner.

Objective To investigate the effect of ketamine, the NMDA receptor antagonist, on inducible nitric oxide gynthase (iNOS) mRNA expression in the spinal cord during the development of morphine tolerance. Methods Thirty Kunming mice weighing 18-22 g were randomly divided into 5 groups ( n = 6 each): A control group received normal saline (NS) only; B chronic morphine tolerance group received subcutaneous morphine 10 mg?kg-1 followed by IP NS after an interval of 30 min, twice a day for 9 days and C, D, E ketamine groups received subcutaneous morphine 10 mg?kg-1 followed by IP ketamine 5 (group C) or 10 (group D) or 20 (group E) mg?kg-1 after an interval of 30 min, twice a day for 9 days. One hour after the last drug administration the animals were decapitated and the lumbar enlargement of the spinal cord was isolated and the iNOS mRNA expression in the spinal cord was detected by RT-PCR. Results Expression of iNOS mRNA was not detectable in group A but increased dramatically in group B. The iNOS mRNA expression was significantly lower in group D and E (ketamine 10 and 20 mg?kg-1 IP) than in group B and C (ketamine 5 mg?kg-1) . Conclusion Ketamine antagonizes the development of chronic morphine tolerance in mice through down-regulation of iNOS mRNA expression in the spinal cord.