Efforts are being made to build up platforms to support basic research of ophthalmology by organizing capable staff,adequate instruments,technical assistance,and by applying practical evaluation and cultivating healthy cultural environment.While significant progress has been achieved with scientific publications and international academic exchanges,the ophthalmology society still has a long way to go to enhance the basic research in the field of eye science.Utilization of modern biomedical tools,technologies and strategies will undoubtedly further improve the efficacy and production in the area of basic research of ophthalmology in this country.

Purpose：To determine the treatments and prognostic predictors of malignant melanoma of the head and neck. Methods：Surgical treatment and outcome was analyzed for 28 patients with malignant melanoma of the head and neck at department of the head and neck of our hospital (1984-1994). Results：23 patients underwent local excised as the first treatment, and local recurrence was demonstrated in 8 (34.8%). Margin of resection of primary melanoma was divided into narrow excision (≤2 cm) and wide excision (＞2 cm), and 5-year survival was 58% and 40%(P＜0.01). According to surgical methods, 5-year survival of WE, WE/END and WE/TLND were 80%, 80% and 39%. Conclusions：An excision with 2 cm margin is safe procedure for primary tumor of malignant melanoma of the head and neck. Neck dissection may not be practiced until neck lymph node is positive. Neck dissections differ from the positions of the primary tumors, and the flaps must be raised preferably superficial to the platysma.

There was never a time in history that so many disciplines and specialists participate and try to lead the clinical management for acute pancreatitis. Chaos remains at bedside, and episodes of under-intervention and especially over-treatment frequently happened. Physicians are easily confused by questions and controversies in management for acute pancreatitis. We have reviewed the articles in acute pancreatitis and made our recommendations based on the latest evidences.

Acupuncture Therapy ; Adult ; Aged ; Fasciitis ; therapy ; Female ; Humans ; Male ; Middle Aged

Objective To prepare and identify monoclonal antibodies (mAb) against histidine-tag (His-tag ) .Methods Balb/c mice were immunized with polypeptides containing 15 histindine(His)-coupled BSA and this fusion was prepared according to con-ventional methods .Indirect ELISA was used to screen the positive clones and limited dilution for further cloning .After purified with Protein G affinity chromatography ,these antibodies for His-tag were detected for antibody titer ,relative affinity as well as subtypes by using ELISA .The specificity of these mAb was identified by ELISA and Western blotting analysis .Double-antibody sandwich ELISA was composed of these mAb paired with the corresponding recombinant protein of specific antibody ,which was used to de-tect the recombinant protein quantitatively .Results 5 hybridoma cell lines stably secreting anti-His-tag IgG1(κ) were screened .A-mong these antibodies ,1-G2 was of the highest affinity ,reaching 1 .27 × 1010 ,which could combine with different recombinant pro-teins containing His-tag in the experiment of ELISA and Western blotting .1-G2 also could be used to detect recombinant PCT pro-tein containing His-tag quantitatively by using double-antibody sandwich ELISA with antibody of PCT ,the sensitivity of detection reached 4 .6 ng/mL .Conclusion The mAb against for His-tag with high specificity ,affinity and secreted stably are successfully prepared .This prepared antibody could be used in ELISA and Western blotting to detect a variety of His-tag .

Objective To find the optimal stimulation parameters of electroacupuncture for incision pain and visceral dragging pain after surgery.Method Databases including the China National Knowledge Infrastructure (CNKI), WanFang (WF), VIP and PubMed were retrieved by using computer. The eligible literatures related to the clinical and experimental studies on electroacupuncture for post-operative pain were summarized and the frequencies of the involved electroacupuncture parameters were counted.Result For incision pain, electroacupuncture was used after surgery, with continuous wave, usually a low frequency ranged 2~15 Hz and a current of 2~5 mA; for visceral dragging pain, electroacupuncture was used before surgery, with sparse-intense wave, alternate frequencies majorly at 2/100 Hz and a medium or low current intensity within patient’s endurance. Conclusion In clinical application, the elctroacupuncture parameters should be chosen carefully according to the type of post-operative pain.

Objective To investigate the effect of surgical intervention combined with steroid therapy on infantile hepatitis(INS) with persistent jaundice.Methods Twenty-two patients (19 males,3 females,aged 2-6 months) with persistent jaundice(therapy group) were admitted into hospital in the period of Jan.2007-Dec.2008.The patients were performed with surgical intervention after they were confirmed with diagnosis as INS.Then,sodium chloride,gentamicin and Dexamethasone were used to irrigate the biliary tract during and after the operation for 14 days.Three days after operation,20 mg,15 mg,10 mg,5 mg of methylprednisolone were administered intravenously to the patients every 3 days,followed with 4 mg/(kg?d) prednisone by oral for 2-3 months.The 17 cases of INS with persistent jaundice were treated with medicine as control(control group).By following-up,the jaundice free and 2 years survival rate of 2 groups were compared by counting the cases of jaundice free and recording the survival time.Results Two cases of 22 patients performed with surgical intervention were diagnosed as biliary atresia and others were INS,90.0% patients were free of jaundice in surgical intervention combined with steroid therapy group,which was higher than that in control group(52.9%,P

Purpose To explore the expression of two epithelial-mesenchymal transition (EMT) regulators,Sry-related high mobility group box 4 (SOX4) and enhancer of zeste homolog 2 (EZH2),in breast cancer tissues and their clinicopathologic significances.Methods The expression of SOX4 and EZH2 was detected by immunohistochemistry of EnVision twostep in paraffin-embedded tissue sections from 241 cases of breast cancer and 126 cases of paracancerous breast tissues.The relationship between their expression and clinicopathologic characteristics was statistically analyzed,and their correlation was also analyzed.Results In breast cancer tissues and paracancerous breast tissues,the high expression rates of SOX4 were 82.2％ and 57.1％ respectively,while the high expression rates of EZH2 were 80.1％ and 47.6％ respectively.Their expression in breast cancer tissues was higher than that in paracancerous breast tissues (P ＜ 0.05).High SOX4 expression was correlated with lymph node metastasis,high TNM stage,and overexpression of HER-2 (P ＜ 0.05),while high EZH2 expression was correlated with high histological grade,lymph node metastasis,ER negativity,EGFR positivity and high poliferation index of Ki67 (P ＜ 0.05).There was a positive correlation between SOX4 and EZH2 expression in breast cancer tissues (rs =0.256,P ＜0.001).Conclusion EMT regulators SOX4 and EZH2 might be responsible for the metastasis of breast cancer,which are upregulated in breast cancer tissues.They may act as potential biomarkers to predict the metastasis of breast cancer.

Based on the analysis on the most common type of scientific research and clinical application of stem cells, including embryonic stem cells , hematopoietic stem cells and mesenchymal stem cells as the breakthrough point, discusses common phenomenon and problems of ethics , scientific research and clinical study the moral life science century new topic, hope scientists, physicians, ethicists, caused by the administrative departments for pub-lic health thought , promote stem cells healthy and steady development .

Objective To study the expression of PTEN,Fas and DCR3 in human gastric cancer and evaluate their effects on carcinogenesis,cell proliferation,invasion and metastasis of gastric carcinoma,as well as clinical significance.Methods The expression of PTEN,Fas and DCR3 were detected by immunohistochemical method (streptavidin-peroxidase) in the tissues of 75 primary gastric carcinomas and 15 gastric ulcers.Results The positive expression rate of DCR3 in gastric carcinoma tissues was significantly higher than that in normal tissues,and the expression of PTEN and Fas was obviously lower than that in normal tissues (P<0.01).The expression of DCR3 was negatively correlated with Fas and PTEN respectively (r=-0.720,P<0.001; r=-0.336,P<0.001).There was a positive correlation between PTEN and Fas.The expression of PTEN,Fas and DCR3 was closely correlated with lymph node metastasis,clinical stage,histological differentiation grade and tumor depth of gastric cancer,but not related to sex,age and tumor size (P>0.05).Conclusion PTEN,DCR3 and Fas have mutual restrictive relationships in the process of invasion and metastasis in gastric cancer.Detecting the expression of PTEN,Fas and DCR3 in gastric cancer is helpful to judge the malignant biological behavior of gastric cancer at a different angle,which may be considered as reliability index in clinic diagnosis,judging curative effect and monitoring the prognosis of gastric carcinoma.