Objective To investigate the clinical characteristics,management and prognosis of choriocarcinorna in postmenopausal women. Methods Six cases of choriocarcinoma in postmenopausal women were reviewed retrospectively. Results Four cases in the 6 patients presented with abnormal vaginal bleeding. All of the six cases received chemotherapy and four cases underwent hysterectomy. Three cases achieved complete remission,one partial remission,one stable condition,and one disease progression.One case died,two cases lost follow-up and three cases survived as follow-up so far. Conclusion The diagnosis of pregnancy-related disease should be considered in the elderly women presenting with abnormal vaginal bleeding.Once diagnosed,chemotherapy should be given as soon as possible. Satisfactory effects can be achieved with a combination therapy of chemotherapy and surgery.

In the paper, the training situation of clinical pharmacist of Chinese medicine was analyzed through the current work situation of clinical pharmacy of Chinese medicine in China. The lack of staff and low level are the undoubted facts. In order to promote clinical pharmacy of traditional Chinese medicine, clinical pharmacists with high quality should be trained. How to train clinical phar-macists of Chinese medicine with high quality, some suggestions were put forward from two aspects of the college education reform and clinical training:①The existing education system should be reformed to establish reasonable higher education for clinical pharmacy of Chinese medicines, including the reform of education system, the curriculum adjustment, practice direction change and so on;②With the attention of all the parties, practical talents of clinical pharmacy of Chinese medicine should be trained in clinics, including the na-tion should start the training for clinical pharmacist of Chinese medicine, hospitals should train practical clinical pharmacist of Chinese medicine and carry out the corresponding work of clinical pharmacy of Chinese medicine, staff should improve self quality and so on.

Objective:To determine if a group psychotherapy programme offers significant benefits to psycho-logical wellbeing for early and mid-term colorectal cancer patients compared to control group.Methods:Totally 67 patients previously diagnosed with colorectal cancer who participated in this study,34 patients accepted an invitation to join the intervention group,received 10 consecutive,weekly sessions group psychotherapy,and 33 patients served as control subject with usual care only.All participants were assessed at baseline and post-therapy (week 10 follow-ing enrollment or equivalent ).Assessment comprised self-completed Cancer Quality-of-life Core Questionnaire (QLQ-C30),Self-Rating Depression Scale (SDS)and Self-Rating Anxiety Scale (SAS).Results:At baseline, 67participants completed questionnaires,and 60 questionnaires returned at post-therapy,from 30 intervention group participants and 30 control group participants.Baseline scores for SAS and SDS were higher in intervention than control group[(42.0 ±7.7)vs.(36.0 ±6.8),(44.4 ±11.5)vs. (38.7 ±9.0),Ps <0.05],while other itemswere not differ(Ps>0.05 ).Post-therapy,intervention group participants reported significantly scores for anxiety, depression and fatigue compared with controlled group.In the comparison of before-post difference between inter-vention and controlled group,the difference of SAS score,SDS score,role function score and fatigue score showed significant discrepancy,intervention group improve significantly (Ps <0.05 ).Conclusion:Group psychotherapy could improve anxiety and depression,for colorectal cancer patients,and may help to improve quality of life,possi-bly through improving role function and decreasing fatigue symptoms.

Objective To construct human single-chain antibody gene library associated with lung adenocarcinoma, and screen lung adenocarcinoma cell A549-specific antibody. Methods The lymphatic tissue near lung adenocarcinoma was used to construct human single-chain antibody gene library. The specific antibody to lung adenocarcinoma cell A549 was screened from the antibody library. Positive clone bacteria were transformed to E. coli HB2151, and their dissolvability was observed. The soluble scFv was purified by affinity chromatography and its relative molecular mass was determined by SDS-PAGE and Western blotting. The specificity of scFv to human lung adenocarcinoma cells was identified with ELISA. Results The phage antibody library of 4.6?107 was constructed successfully. Single-chain antibody of human lung adenocarcinoma was enriched. The ratio of yield was increased gradually, 181 times higher after 5 rounds of panning than after the first round of panning. SDS-PAGE and Western blotting results showed soluble scFv' molecular mass was about 30 000. ELISA showed dissolved antibody had high specificity to bind A549 cells, not MDA-MB-435. Conclusion The single-chain antibody gene library of human lung adenocarcinoma was constructed successfully, and specific antibodies to lung adenocarcinoma were screened, providing the basis for single-chain antibody radionuclide imaging and treatment.

Objective To analyze the distribution of common pathogenic bacteria and their drug resistance ,in children with bron‐chial pneumonia ,and provide reference for making antimicrobial treatment strategies .Methods 857 hospitalized pediatric patients with bronchopneumonia from January to December 2014 were enrolled in the study ,bacterial culture and drug susceptibility test were performed ,and the results were analyzed .Results In the 857 pdiatric patients ,the positive rate was 36 .87% (316/857) .A‐mong them ,175 cases were gram positive bacteria(20 .42% ) ,141 cases were gram negative bacteria(16 .45% ) ,140 cases were Streptococcus pneumoniae(16 .34% ) ,70 cases were Haemophilus influenzae(8 .17% ) .The susceptibility of Streptococcus pneu‐moniae to penicillin with enzyme inhibitors ,levofloxacin and vancomycin was the highest(higher than 90 .00% ) ,followed by the third generation cephalosporin(non meningitis) ,to which the sensitivity rate were above 80 .00% .The susceptibility of Haemophi‐lus influenzae to the third generation cephalosporins ,ampicillin/Sulbactam ,levofloxacin and imipenem were the highest(more than 90 .00% ) ,while the resistance to ampicillin and cotrimoxazole was 80 .00% .Conclusion Gram positive bacteria are the most im‐portant pathogenic bacteria ,and Streptococcus pneumoniae is the most common bacteria in bronchopneumonia .In clinical treatment , the results of drug sensitivity test should be paid attention to ,so as to adjust the treatment plan and achieve the ideal effect .

10.3969/j.issn.2095-4344.2013.23.005

BACKGROUND: Intramyocardial transplantation of autologous umbilical cord-derived mesenchymal stem cells for repair of myocardial tissue damage is paid increasing attention in the cardiovascular field. OBJECTIVE: Human umbilical cord mesenchymal stem cells were isolated and cultured. Passage 2 human umbilical cord mesenchymal stem cells were treated with various concentratins of 5-azacytidine (2.5, 5, 10, 20, 40, 80 μmol/L) for 24 hours . After removal of 5-azacytidine, cells were cultured for another 4 weeks. RESULTS AND CONCLUSION: Before 5-azacytidine treatment, filament-like structures or particles were not observed in the cells, but the amount of cytoplasm was less and uniform, nuclear/cytoplasm ratio was high, cells exhibited typical fusiform appearance and grew in a swirl-like manner, and nucleolus was obvious. After treatment with 5-azacytidine for 24 hours, some cells died in each group, and typical fusiform appearance turned into stick-like or column-like appearance, especial y in the 40 and 80 μmol/L 5-azacytidine groups. Reverse transcription-PCR results showed that atrial natriuretic peptide and α-skeletal actin gene expression levels were detected on human umbilical cord mesenchymal stem cells after treatment with 2.5 or 40 μmol/L 5-azacytidine for 4 weeks or with 5, 10, 20 μmol/L 5-azacytidine for 1, 2, 3 and 4 weeks. These findings suggest that 5-azacytidine-induced human umbilical cord mesenchymal stem cells express the specific gene of myocardiocytes.

PurposeTo investigate the independent risk factors of refractory hypertension for effective screening and treatment.Materials and Methods 142 patients with refractory hypertension underwent renal angiography, morbidity of renal artery stenosis (RAS) and angiography results were analyzed using univariate and multivariate Logistic regression.Results Thirty-eight cases of RAS were identiifed with incidence of 26.8%. Univariate analysis indicated that diabetes, peripheral artery disease and coronary heart disease were the predictor for RAS (P<0.05 orP<0.01). Multivariate regression analysis demonstrated that peripheral artery disease (OR 5.011, 95%CI 2.17-8.93,P<0.001) was independent risk factors for RAS. Diabetes and coronary heart disease were not independent risk factors.Conclusion Peripheral artery disease is independent risk factor for RAS among patients with refractory hypertension, which can serve as a screening index of renal angiography or renal angioplasty and stenting for RAS.

[Objective] To compare the effects of Radix Astragali (RA) and triperygium glucosides (TG) on glomerular mesangial cells (GMC) proliferation and interleukin-6 (IL-6) secretion. [Methods] GMC were cultured with in-vitro cell culture technology. Serum containing RA and serum containing TG were prepared with serum pharmacological methods. GMC proliferation was detected by methylthiazolyltetrazolium (MTT) assay and IL-6 level by enzyme-linked immunosorbent assay (ELISA) to evaluate the effect of low-, moderate- and high-dosages of RA-containing serum and TG-containing serum under the condition of GMC over-proliferation. [ Results] Different dosages of RA-containing serum and TG-containing serum inhibited GMC proliferation and IL-6 secretion in a dose-effect manner, and the inhibitory effect of high- and moderate-dosage TG and high-dosage RA was stronger. [Conclusion] Under the condition of in-vitro GMC over-proliferation, RA has the similar inhibitory effects on GMC proliferation and IL-6 secretion with TG. The combined treatment of RA and TG can reduce the dose of TG and probably has an action in increasing effect and reducing toxicity.

Objective To find a useful index for real-time detecting of speech endpoint and improving the performance of speech processing under low SNR by analyzing fluctuation complexity of speech signals. Method The influence of state space partition method, window size and partition numbers on detecting performance was analyzed. The comparison experiments of speech signals corresponding to different SNR and noise type was designed using the measure of complexity behaviors based on the information gain.Result It was found that fluctuation complexity was more effective in detecting low-SNR speech than spectral entropy. Conclusion Fluctuation complexity is a valid feature to make speech/non-speech decision for the low SNR cases. The presented method can achieve robust performance and has a good real-time behavior.