Aim To investigate effects of 1?,25-Dihydroxyvitamin D_3 on the growth inhibition,proliferation of K562 cells and to explore its mechanism. Methods The expression of vitamin D receptor(VDR) was indentified by indirect immunofluorescent stainings.Cell growth,proliferation,apoptosis and cells cycle were evaluated by MTT assay,acridine orange/ethidium bromide(AO/EB) and flow cytometry PI staining.The enzymatic activity of the caspases-3 class of K562 cells was determined by colorimetric assay.Results ① VDR was present in K562 cell nucleus;② 10~(-8) mol?L~(-1) 1,25(OH)_2D_3 could markedly inhibit K562 cells growth and induce cells apoptosiswith most of cells being arrested in G_2/M phase.The ratio of apoptosis increased from 4.1%(control group) to 26.5%(treatment group),P

Diplopia ; therapy ; Humans ; Ophthalmologic Surgical Procedures ; Orbital Fractures ; diagnostic imaging ; epidemiology ; surgery ; Prevalence ; Tomography, X-Ray Computed

Hepatoblastoma(HB) is the most common malignant hepatic tumor in the pediatric population,almost exclusively in infants and toddlers, which accounts for 50％ ～ 60％ of the liver tumors.Now, the comprehensive treatment program of HB in children includes surgery, chemotherapy, radiotherapy, and so on.Liver transplantation and autologous peripheral blood stem cell transplantation could also be applied to advanced or refractory hepatoblastoma in children.The high sensitivity of childhood hepatoblastoma to chemotherapeutic agents,makes chemotherapy as one of the main treatment modalities.This review mainly discusses chemotherapy treatment of HB in children.

Objective:To detect the caries activity of orthodontical treated and untreated adult.Methods:The study population consisted of 30 adults whose age are over twenty,the control group includes 30 adults who did not accept any orthodontical treatment.The detective methods were enumeration of streptococcus mutans in saliva,ability of plaque to produce acid and salivary buffering capacity.Student's test was used to analyse the statistical difference.Results:The results showed that the number of S.mutans and lactobacillus on the untreated group are much lower than that of the orthodontical group;the salivary pH,salivary buffering capacity and ability of germ to produce acid are similar between orthodontical treated and untreated adult.Conclusion:This study suggests that the orthodontical treated adult are more sensitive to dental caries than untreated adult.

Objective:To eveluate the clinical effects of treating dentine hypersensitivity with three kinds of desensitizer(Prime & Bond NT,GLUMA desensitizer and 75% sodium fluoride glycerin).Methods:324 teeth with dentin hypersensitivity were divided into three groups.Group A:Prime & Bond NT adhesive.Group B:GLUMA desensitizer.Group C:sodium fluoride.Results:Instant,effective rates in group A and group B were significantly higher than that in group C(P0.05).After three months,effective rates in group A was significantly higher than that in group B(P

AIM:To study the extraction of Schisandra Chinensis (Turcz) using supercritical carbon dioxide as extracting ageat. METHODS: The study was carried out through orthogonal test design,the content of schizandrin from Schisandra Chinensis (Turcz) was selected by 3 influencing factors, extract temperature, extract pressure and separation temperature. RESULTS:The optimal condition for the extraction as A3B2C1 Schisandra Chinensis (Turcz) was extracted by supercritical carbon dioxide, extract pressure was 25MPa, extract temperature was 50?C, separation was 7 MPa, separate temperature was 60?C, extract time was 2.5h and runing amount of CO2 was 15kg?h -1. CONCLUSION:It is feasible that supercritical carbon dioxide is used in the preparation of Schisandra Chinensis (Turcz).

Objective:To investigate the oral health status of elderly people in Chongqing,and research incidence of caries and periodontal diseases in elderly people,in order to supply enough information for constituting programme of oral health care for elderly people. Methods:Based on oral health survey methods set down by World Health Organization and second national oral health survey standards,proportional stratified randomized sampling was employed in 2005. The total 762 samples from three cities and three countries were examined. Results:The coronal caries rate was 75.95%;the mean number of coronal caries was 14.32;the root caries rate was 58.40%,and the mean number of root caries was 4.24. Gingival bleeding rate was 98.82%;periodontal pocket rate,96.06%,and periodontal attachment loss rate,41.77%. Conclusion:Caries prevalence rate is high and periodontal health status is poor. These results suggest that oral care service should be established to improve the oral health status of elderly people in Chongqing and prevent epidemic of oral diseases.

Objective:To observe the effect of high zinc concentrations on contents of iron and zinc and expression of their regulating mRNA in Caco-2 cells.Methods: Caco-2 cells were used as model of human small intestinal enterocytes and were treated for 24 h with 4,50,100 and 200 ?mol/L of zinc.The zinc and iron contents were determined by atom absorption spectrophotography and the expression of DMT1,IREG1,ZnT1 and hZIP4 mRNA were determined by RT-PCR(the level of GAPDH was taken as internal control).Results: The zinc content in Caco-2 cells was increased 24 h after treatment with zinc,and reached the peak when exposed to 50 ?mol/L zinc.The iron content decreased with the increase of zinc concentration.Zinc supplement increased the expressions of DMT1,IREG1 and ZnT1 mRNA but decreased the expression of hZIP4 mRNA.Conclusion: It is suggested that zinc supplement can increase the contents of zinc but decrease the contents of iron in Caco-2 cells.There may be interactions between iron and zinc in the intestinal lumen.

China ; Fracture Fixation, Internal ; education ; Humans ; Maxillofacial Injuries ; therapy ; Societies, Medical ; Surgery, Oral ; education

Objective To discuss the effect on promoting the progress of the stage of labor and the natural delivery as the parturient adopts the different delivery position in the first and the second stage of labor. Methods One hundred and fifty-two cases of delivery primiparas of cephalic presentation whose pregnancy was from 37 to 41 weeks were enrolled and divided into two groups randomly. In the observation group (80 cases) ,the free positions of delivery like walking,squating,lateral position,half lying and others could be selected in the first stage of labor, the semi-reclining position was selected in the second stage of labor and the lithotomy position was adopted when the head was visible on vulvae gapping. The parturient in the control group (72 cases) selected the decubitus (alternation between prostration and lateral position) in the first stage of labor and the parturient selected routine lithotomy position of supine position in the second stage of labor. The progress of labor and the comfort of the parturient in the two groups were observed. Results In the observation group, time spent in the first stage and second stage were (361. 83 ± 58. 02) mins and (36. 39 ± 11. 97) mins, and (398.21 ±59. 72) mins in the total birth process, which were significantly shorter than those of (560.85 ± 131. 98)mins and (58.11 ± 15.44) mins, and (618. 96 ± 139. 61) mins, respectively (t = 12. 24,9. 74 and 12. 90, Ps <0. 01). There were 13 and 25 cases experienced tingle in two hours after delivery, and 2 and 10 cases in 12 hs after delivery, in the observation and control group, respectively. The comfort of parturient in the observation group was higher than that in the control group (x2 = 6. 90, P < 0.01; x2 = 5. 28, P < 0. 05)respectively. Conclusion The free position in the first stage of labor and the semi-reclining position in the second stage of labor can shorten the birth process effectively and relieve the discomfort after the delivery.