Objective: To clarify Radix et Caulis Acanthopanacis Senticosi decoction (DAS) via Chitosan Flocculation. Methods: The operating conditions were optimized and the results were compared with that of ethanol subsiding. Economic budget was carried out. Results: The optimized conditions were: 28.6mL/L Chitosan flocculating agent was added at 30 ?C and pH 5.36. Conclusion: Chitosan coagulating method was economic and efficient on the clarification of DAS and can replace ethanol subsiding method.