ObjectiveTo develop a new nested PCR to detect human papillomavirus (HPV) DNA in lesions of extragenital Bowen's disease.Methods DNA was extracted from the lesions of 41 patients with extragenital Bowen's disease and suhjected to the amplification of HPV by a nested PCR.Five primers,including CN1FR,CN2FR,CN3FR,CN4FR and CN5FR,were designed and used in the second round PCR.ResultsBased on the ClustalX analysis,69 HPV subtypes,including mucosal types,cutaneous types and epidermodysplasia verruciformis-associated types,could be amplified by using the 5 designed primers.The detection limit varied from 10-3 to 10-2 copies of HPV DNA for this PCR.Of the 41 lesional specimens,5 were positive for HPV DNA,including 3 cases of high-risk HPV types(2 cases of HPV 16,1 case of HPV 33) and 2 cases of cutaneous HPV types(1 case of HPV 27 and 1 case of HPV 76).ConclusionsThe improved nested PCR is highly sensitive and specific for the detection of HPV DNA in lesions of extragenital Bowen's disease.The development of extragenital Bowen's disease may be associated with the infection with high-risk mucosal HPV types in some patients.

Objective:In order to explore the anti inflammatory mechanisms of ? melanocyte stimulating hormone (? MSH), the effects of ? MSH on the production of NO and proinflammatory cytokines in astrocytes induced by LPS were investigated Methods:Rat brain astrocytes cultured in vitro were stimulated with LPS or given ? MSH with LPS stimulation NO produced in astrocytes was tested with Griess reagent IL 1, IL 6 and TNF ? secreted from astrocytes were examined by MTT assay The expression of macrophage migration inhibitory factor (MIF) mRNA was examined with semiquantitative RT PCR analysis Results:The production of NO, IL 1, IL 6, TNF ? and the expression of MIF mRNA were significantly increased in astrocytes stimulated with LPS If giving ? MSH with LPS stimulation, the production of NO, IL 1, IL 6, TNF ? and the expression of MIF mRNA were markedly decreased Conclusion:[WT5”,6BZ]It is suggested that the inhibitory actions of ? MSH on the production of NO and proinflammatory cytokines in astrocytes are related to the inhibitory effects of ? MSH on inflammation in central nervous system

Objective: To investigate the role of T cell in the antitumor immune responses induced by MIF gene-modified tumor vaccine. Methods: MIF gene was transferred into FBL3 erythroleukemia cel l by adenovirus carrier and a new type of tumor vaccine was prepared. The chang es of the number and the function of T cell in spleen and lymph node was observe d. Results: After the mice were immunized with MIF gene-m odified FBL3 vaccine, the number of lymphocyte in spleens and lymph nodes increa sed markedly and the specific CTL activities of splenocytes also increased great ly. FACS analysis showed that the CD3+,　CD4+,　CD8+ T cells and CD28 posi tive cells in draining lymph nodes of MIF-FBL3 group mice increased more marked ly than that of control groups. When the wild type FBL3 cells were injected into the mice immunized with MIF gene-modified FBL3 vaccine, the growth of tumors w ere obviously inhibited and the survival rate of the mice was increased. Conclusion: It is suggested that MIF gene-modified tumor vaccine can induce specific antitumor immune responses mediated by T cells and may be a candidate for gene therapy of tumor.

Objective: To study the effects of TCFC on proliferation and osteogenic action of osteoblast in neonatal rat calvaria cultures in vitro. Methods: Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion. The proliferation and collagen synthesis were assayed by 3H TdR and 3H proline incorporation. The activity of ALP was measured by p nitrophenyl sodium phosphate assay. The ipriflavone was used as positive control drug.Results: TCFC significantly promoted bone cell proliferation, alkaline phosphatase activity and collage synthesis.Conclusion: TCFC had the anti osteoporosis action by promoting osteoblast proliferation, ALP activity and collage synthesis.

Objective To evaluate the effect of enclosed safety blood sampling device in prevention of arterial catheter-related bloodstream infection.Methods Totally 510 patients who received successful radial and brachial artery cannulation in ICU of the Second Hospital of Jiaxing between June and December 2016 were selected according to the table of random number and randomly and equally divided into a control group and an experimental group. The patients in the control group received conventional blood sampling with arterial transducers, while the patients in the experimental group received blood sampling with Safedraw enclosed safety blood sampling devices. Their incidence of complications and AC-BSI related to arterial catheter were observed. Results The incidence of complications related to arterial catheter of the patients in the experimental group was 31.76%, lower than that of the patients in the control group, 42.74% (x2=6.576,P<0.05); the incidence of AC-BSI of the patients in the experimental group was 3.92%, lower than that of the patients in the control group, 8.23% (x2=4.156,P<0.05).Conclusions Enclosed safety blood sampling device used in pressure measurement for artery cannulation can effectively reduce the incidence of complications and AC-BSI related to arterial catheter, thus worthy of clinical expansion.

OBJECTIVETo explore the biomarkers for monitoring trinitrotoluene (TNT) exposure, the relationship between TNT hemoglobin adducts and TNT exposed level.

METHODHemoglobin adducts (4A-Hb and 2A-Hb) were determined by GC-MS in 25 TNT exposed workers. TNT exposed level was evaluated by determining skin contaminated and inhaled TNT levels. The correlation between hemoglobin adducts level and TNT exposed level was analyzed.

RESULTSThere was a correlation between total TNT exposure level, especially skin exposure level, and 4A-Hb or 2A-Hb content. No significant difference was found between the slopes and intercepts of lin ear equation of (4A-Hb) vs TNT exposed level and linear equation of (4A-Hb +2A-Hb) vs TNT exposed level (P > 0.05).

CONCLUSIONSkin contamination is the major role of TNT exposure. TNT exposed level can be evaluated by determining the content of both 4A-Hb and 2A-Hb, and 4A-Hb is more suitable for monitoring TNT exposure.

Environmental Monitoring ; methods ; Hemoglobins ; metabolism ; Humans ; Occupational Exposure ; Skin ; drug effects ; Trinitrotoluene ; metabolism

Objective To explore the effects of multidisciplinary teamwork continuity of care for community-dwelling patients with advanced renal cell carcinoma treated with targeted drugs.Methods A total of 80 patients with stage IV renal cell carcinoma treated in our hospital from February 2014 to February 2017 were enrolled using convenience sampling method,and were randomly divided into the experimental group and the control group using random number table,with 40 cases in each group.Both groups were given routine nursing care,and multidisciplinary teamwork continuity of care was adopted in the experimental group additionally.Results One month after intervention,the patients' score of medication compliance questionnaire in the experimental group was significantly higher than that in the control group(P＜0.05);three months after intervention,the quality of life(QLQ-C30) score of the experimental group was significantly improved than that of the control group(P＜0.05),and the number of rehospitalization was significantly less than that in the control group(P＜0.05).The number and severity of adverse effects in the experimental group were lower than those in the control group,and no case of stopping medication or decreasing doses occurred.Conclusion The multidisciplinary teamwork continuity of care model can enhance health status of community-dwelling patients,improve medication compliance and quality of life,and reduce readmission.

Objective To construct multimedia intensive care knowledge base in DoCare information system, and provide an accurate, rapid and effective method to obtain nursing knowledge and technology for nurses to meet the needs of critical care practice. Methods The specific needs of ICU nurses in the intensive care knowledge base were investigated, and the intensive care knowledge base was constructed combined with other company. Results The main contents of intensive care knowledge base were ICU nursing management system, responsibilities of ICU nurses, ICU workflows, ICU common operating procedures, prevention and treatment of ICU common operating complications, the rescue process of ICU common diseases, knowledge of commonly used drugs in ICU, significance and nursing of common test items in ICU, prevention and control knowledge of hospital-acquired infection in ICU, commonly used nursing assessment tools in ICU and methods of use, differentiation and analysis of common arrhythmia, health education of ICU diseases, various nursing checklists, emergency plans, and ICU new knowledge and related guidelines. The text contents and operation videos were the main forms of the intensive care knowledge base, and the functions were searching, location searching and others. Conclusions The intensive care knowledge base is an effective way for ICU nurses to obtain new knowledge and skills, and make clinical decision quickly and accurately.

Objective To establish the integrated prevention and control guide for multidrug-resistant organism (MDRO), guide nursing staff to strengthen prevention and control for MDRO and to compare the rate of implementation in preventive and control measures along with the discovery rate of MDRO before and after implementing guide.Methods The main problems of prevention and control management for MDRO were analyzed and induced with the methods of literature search, interviews with medical staff in ICU, referring to relevent clinical data and so on. The prevention and control guide was formulated according to expert consensus on MDRO, hospital infection management system of MDRO, clinical practice demand and so on. The prevention and control guide was implemented involving carrying out evidence-base training, building up prevention and control process, quality control and so on from July 2015 to June 2016. All patients of ICU in the Second Hospital of Jiaxing between July 2014 and June 2016 were divided into two groups before (n=622) and after (n=604) implementing prevention and control guide by purposive sampling. The discovery situation of MDRO was investigated. Besides, the implementation situation of preventive and control measures was evaluated by selecting 380 patients and 375 patients from two groups separately.Results After implementing prevention and control guide for MDRO, the rate of implementation in preventive and control measures (90.40%) significantly improved compared to that before implementation (70.00%) along with the discovery rate of MDRO (8.77%) decreased compared to that before implementation (16.08%) with significant differences (P<0.05). Conclusions The application of prevention and control guide for MDRO can effectively decrease the discovery rate of MDRO in ICU, make prevention and control work of MDRO more scientific, standardized, systematized and have a good clinical guidance role for prevention and control for MDRO of critical patients.

Objective To explore the application effect of "world cafe" mode in the summary of nursing quality.MethodsFrom September 2015 to December 2015,the nursing department discussed the topics of nursing quality summary according to the "world cafe" mode,finally the duration of nursing quality discussion, the structural proportion of participants and the satisfaction of all participants were taken as observation indexes to compare with the traditional nursing quality review meeting held during March to June 2015. According to the "world cafe" mode, the discussion table of nursing summary was established nursing quality,the contents of nursing quality discussion were standardized,and participants of the seminar and the time were made. ResultsAfter the application of "world cafe" mode,the total conference time shortened to 1.5 h from 3 h before the application,and the repeat situation of participants′ statement and the rectification views were obviously improved (χ2=34.737,5.963;P<0.05). After the application of "world cafe" mode,participants were mainly clinical nurses,which accounted for 53.4%,and the structural situation of participants was significantly different (χ2=27.086,P<0.05). There were significant differences in satisfaction before and after the application of "world cafe" mode (χ2=4.706,P=0.03).ConclusionsThe application of "world cafe" mode fully shows the divergent thinking of clinical nurses,and embodies the idea of improving nursing quality of nurses at different levels,which can discover the key problem of nursing quality,provide practical measures for the improvement of nursing quality in next stage,set stage for the implementation of nursing work,shorten the conference time and improve the work efficiency and satisfaction of nurses. This method will have broad research and application prospect as a means to improve the nursing quality.