Objective To investigate related factors of hypersensitivity reactions in patients undergoing paclitaxel chemotherapy for cervical cancer and provide measures for prevention of the side effects.Methods A ret-rospective survey was taken to analyze the clinical data from 186 cervical cancer patients who accepted paclitaxel chemotherapy from January 2013 to December 2014.SPSS 17.0 was used for single factor analysis and multi -factor logistic regression analysis.Results Among the 186 cervical cancer patients enrolled in this study,34 cases occurred hypersensitivity reactions.The incidence rate of anaphylaxis was 18.3%.Both eosinophil count of more than 0.2 × 109 /L(χ2 =4.171,P <0.05)and history of drug allergy(χ2 =10.667,P <0.01)were not merely risk factors of anaphylaxis,but also independent risk factors(OR =4.691,2.257,all P <0.01 ).Conclusion The incidence of hypersensitivity after paclitaxel chemotherapy in cervical cancer patients is high.Eosinophil count ≥ 0.2 ×109 /L before chemotherapy and history of drug allergy are not merely risk factors of anaphylaxis,but also independent risk factors,which require more attention.

Objective To study the effects about using separated bed transfer to handle orthopedic patients.Methods According to the characteristics of carrying orthopedic patients,separated bed transfer was developed and made followed by trials in trial departments.A questionnaire survey of 214 patients and 27 users was completed and the related situation using separated bed transfer to handle orthopedic patients was surveyed,the results were compared with those of dead lift.Results Compared with traditional dead lift,there was obvious difference in the number of handling participators[≥5 persons:6.07％ (13/214) vs.21.96％(47/214);4 persons:35.51％ (76/214) vs.48.13％(103/214)],patients' comfort during handling [28.97％ (62/214) vs.84.11％(180/214)],x2=43.027,154.381,all P ＜ 0.05.Conclusions It can reduce the number of participators and patients' pain when handling patients using separated bed transfer.It is widely used with lower cost,which is worth being promoted in clinic.

Objective To investigate the prevalence rate of nosocomial infections and the use of antibacterial agents in Zhejiang Cancer Hospital.Methods The cross-sectional survey on nosocomial infections was conducted among inpatients in Zhejiang Cancer Hospital on August 11,2011 and June 19,2012.The differences in prevalence rates of nosocomial infections,purposes of antibacterial agents use (for treatment,for prevention,or for both treatment and prevention),methods of medication (single or combined medication),and pathogen detections between 2011 and 2012 were analyzed with x2 test.Results There were 854 and 886 patients enrolled in the study in 2011 and 2012,and the prevalence rates of nosocomial infections were 5.27％ (45/854) and 5.08％ (45/886),respectively (x2 =0.186,P ＞ 0.05).Lower respiratory tract infection was the most common nosocomial infections both in 2011 and 2012,which accounted for 28.89％ and 36.96％ of all infections,respectively; and there was no significant difference in infection sites between 2011 and 2012 (x2 =1.415,P＞0.05).Totally 154 out of 854 patients (18.03％)in 2011 and 186 out of 886 patients (20.99％) in 2012 received antibacterial agents,and single medication was the most common.There were no significant differences in the purposes and methods of medication between 2011 and 2012 (x2 =1.994 and 3.042,P ＞ 0.05).Pathogen detections were performed in 56 out of 59 patients (94.92％) and 57 out of 65 patients (87.69％) who received antibacterial agents for treatment in 2011 and 2012,respectively.The most common pathogens were Escherichia coli,Pseudomonas aeruginosa,and Acinetobacter baumanni.Conclusion The prevalence rate of nosocomial infection in Zhejiang Cancer Hospital is of the satisfactory level,which is due to the good management of antibacterial agents use.

To study the alteration of FHIT gene in tongue carcinoma Tca8113 cell line, total RNA of Tca8113 cells was extracted. The transcript of the FHIT gene of the Tca8113 cell line was detected with nest RT-PCR, and DNA was sequenced. The result showed that abnormal transcript (about 247 bp) of FHIT gene was detected in the Tca8113 cell line. The sequence analysis of the aberrant cDNAs revealed deletions of exons 1-8. Therefore, the deletion of the FHIT gene in Tca8113 cell line might support the hypothesis that the FHIT gene alteration is involved in the development of tongue carcinoma.
Acid Anhydride Hydrolases ; genetics ; Base Sequence ; Gene Deletion ; Genes, Tumor Suppressor ; Humans ; Molecular Sequence Data ; Neoplasm Proteins ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA ; Tongue Neoplasms ; pathology ; Tumor Cells, Cultured

Objective:To study the levels of serum ferritin (SF) in preterm infants of different gestational ages and analyze the influencing factors.Method:From October 2018 to October 2020, preterm infants hospitalized in our hospital were included for prospective study. According to the gestational age, the infants were assigned into 3 groups: 34~36 w group, 32~33 w group and <32 w group. The SF levels were compared among the groups and the influencing factors were analyzed.Result:A total of 919 preterm infants were included, including 481 in the 34~36 w group, 293 in the 32~33 w group and 145 in the <32 w group. The incidence of Cesarean section in the <32 w group was lower than the other two groups ( P<0.001). The incidence of twin birth in the 32~33 w group was higher than the other two groups ( P<0.05). No significant differences existed in gender among the three groups ( P>0.05). The serum SF levels in the 34~36 w group, 32~33 w group and <32 w group were (240.1±167.4), (216.2±137.0) and (204.4±112.8)μg/L, respectively. The serum SF levels in the 34~36 w group were higher than the other two groups ( P<0.05). No significant differences existed in serum SF levels between the 32~33 w group and the <32 w group ( P>0.05). Multiple　linear　regression　analysis　showed　that SF levels were lower in premature infants with small gestational age and Cesarean section delivery ( P<0.05). Conclusion:SF levels in premature infants with small gestational age and Cesarean section delivery are lower and more monitoring of SF are needed.

Objective To measure the reliability and validity of the Chinese version of Patient Activation Measure(PAM)and to explore its applicability in assessment of self-health management among lumbar degenerative disease patients scheduled for lumbar spinal fusion.Methods From January to June 2017,we selected 120 lumbar degenerative disease patients who had a right mind,none communication disorders and were treated at a Class Ⅲ Grade A hospital in Suzhou as well as scheduled for lumbar spinal fusion as subjects by convenience sampling.All of the patients were investigated with the questionnaire involving the general information questionnaire,the Chinese version of PAM and Self-Efficacy for Managing Chronic Disease Scale(SEMCDS).Psychometric characteristics of the Chinese version of PAM were assessed by indexes,such as item analysis,internal consistency reliability,retest reliability,content validity,construct validity and criterion-related validity.Results A total of 120 questionnaires were sent out and all of them were collected with 100.0％for the effective recovery rate.The Chinese version of PAM included 13 items and 4 levels.Its standardized total score ranged from 33 to 100 and the average score was(50.25±11.33).There were 59 patients(49.2％)withthe score less than or equal to 47.0 belong to the first level of activation which indicated they were not aware the importance of self-health management;22 patients(18.3％)the score between 47.1 and 55.1 belong to the second level of activation which indicated they lacked knowledge and confidence of self-health management;31 patients(25.8％)with the score from 55.2 to 67.0 belong to the third level of activation which indicated they began to take action of self-health management;8 patients(6.7％)with the score greater than or equal to 67.1 belong to the fourth level of activation which showed they insisted on life style of self-health management even facing pressure.The Cronbach's α of the whole scale was 0.835.Guttman split-half reliability and retest reliability was 0.736 and 0.878 respectively.The content validity was 0.923 with it of each item ranging from 0.833 to 1.Exploratory factor analysis extracted four common factors which were renamed as the awareness of self-health management for disease,preoperative disease-related knowledge,postoperative prevention ability and the belief to insist on healthy life style transformation according to the rehabilitative characteristics of lumbar spinal fusion with 63.75％for the cumulative variance contribution rate.Spearman rank correlation coefficient in criterion-related validity was 0.689 between the Chinese version of PAM and the SEMCDS.Conclusions The Chinese version of PAM has good reliability and validity.It could be an assessment instrument of self-disease and health management activation for lumbar degenerative disease inpatients scheduled for lumbar spinal fusion in China.Clinic workers could preliminarily screen potential high-risk patients who may undergo unsatisfactory postoperative rehabilitation based on the level of activation and formulate individualized nursing intervention,reasonably distribute medical resources and improve patients satisfaction.

Objective? To understand patient motivation and its influencing factors prior to posterior lumber interbody fusion surgery. Methods? Using convenient sampling method, a total of 200 patients who will undergo posterior lumber interbody fusion surgery due to degenerative disc disease were selected from 3 ClassⅢ Grade A hospitals in Suzhou. General Condition Questionnaire, the Chinese version of Patient Activation Measure (PAM), Hospital Anxiety and Depression Scale were used in the survey. Univariate analysis and multiple linear regression analysis were used to explore the influencing factors of patient motivation. The correlation between patient activation and anxiety and depression were analyzed by Spearman rank correlation. Results? The median and interquartile range of PAM is 48.90 and 10.30. The univariate analysis showed that patient motivation was significantly influenced by patient gender, age, education, marital status, employment status, per-capita income, chronical disease, anxiety and depression (χ2/Z=-3.433, 29.882, 56.322, 8.852, -2.785, 54.092, -3.714, 13.031, 16.712; P＜0.05). Multiple linear regression analysis showed that marital status, education, depression, income, age were the main influencing factors (R2=0.396, P＜ 0.05). Conclusions? Patient motivation needs to be further improved. Intervention strategies can be designed to motivate patients and improve post-operative outcomes.

Objective:To investigate the effect of quality improvement (QI) project on delayed cord clamping (DCC) implementation in very preterm infants.Methods:This study retrospectively collected the clinical data and assessed the QI indices of very preterm infants born in the Women and Children's Hospital of Chongqing Medical University and transferred to the Neonatology Department from January 2017 to January 2021. The indices for QI assessment included three types: (1) process indices: the implementation rate and timing of DCC; (2) outcome indices: hemoglobin level and hematocrit, etc; (3) balancing indices: the proportion of neonates requiring endotracheal intubation in the delivery room and chest compressions, Apgar score body temperature, and blood pH value on admission, etc. There were four phases for the implementation of QI, the pre-QI period (January to December of 2017), the QI period (January to December of 2018), the post-QI period (January to December of 2019), and the sustained-QI period (January 2020 to January 2021). The QI project was performed since August 2018. Control charts or statistical tests were used for statistical analysis.Results:(1) Process indices: After the implementation of the QI project, the practice of DCC increased from 0 to 82.2%, and the timing of umbilical cord clamping was from 0 s to a delay of 47.1 s. (2) Outcome indices: The levels of hemoglobin in the QI period, the post-QI period, and the sustained-QI period were significantly higher than those in the pre-QI period [(202.22±28.84), (210.10±33.52), (210.52±32.27) g/L vs. (187.94±35.29) g/L; F=8.61, P<0.001]. The hematocrit values in the post-QI period and the sustained-QI period were significantly higher than those in the pre-QI period [(58.99±8.71) %, (60.18±8.06)% vs. (55.41±9.17)%; χ2=5.13, P=0.002]. The other indices showed no statistical differences in different phases. (3) Balancing indices: The proportions of neonates receiving endotracheal intubation in the delivery room in the post-QI period and the sustained-QI period were significantly lower than those in the pre-QI period [16.0% (19/119), 13.2% (25/191) vs. 42.3% (30/71); χ2=29.08, P<0.001]. The body temperature on admission increased gradually through the four periods [pre-QI period: 35.3 ℃ (34.5-36.1 ℃); QI period: 36.0 ℃ (34.0-37.7 ℃); post-QI period: 36.0 ℃ (35.6-37.4 ℃); sustained-QI period: 37.0 ℃ (35.9-38.1 ℃); H=277.88, P<0.001]. The blood pH value on admission in the sustained-QI period [7.32 (6.85-7.50)] was significantly higher than those in the other three periods [7.26 (7.07-7.46), 7.26 (7.04-7.43), and 7.25 (6.91-7.49); H=34.46, P<0.001]. Conclusion:The interventions in this QI project significantly increase the practice of DCC and enable a longer DCC in very preterm infants.