[Objective]To investigate the biomechanical properties of transpedicular impacted grafting(TPIG) after bone loss calculation in the treatment of thoracolumbar compression fracture.[Method]Six fresh human thoracolumbar spine(T12-L2) specimens were tested as 6 models: normal control(group A),compression fracture(group B),TPIG after bone loss calculation(group C),transpedicular grafting(TPG)(group D),TPIG after bone loss calculation + pedicle fixation(group E),TPG + pedicle fixation(group F).Biomechanical analysis was carried out to test the intensity,stiffness,stability and torsion properties between different groups.[Result]The intensity,stiffness and stability of vertebral body and intervertebral disc in group E were higher than those in group F,with significant difference(P