China ; Dental Implants ; Health Services Needs and Demand ; Humans ; Periodontics ; manpower

Objective To determine the function and binding properties of SR-A in HMC.Methods A human mesangial cell line(HMCL) with high expression of type A SCR(HMCL-SCR) was established after stable transfection of expressive vector with cDNA encoding SR-A.Uptake of fluorescence Dil-labeled acetylated low density lipoprotein(Dil-Ac-LDL) and oxidized LDL(Ox-LDL) by HMC were evaluated by fluorescence microscopy,con-focal microscopy and Oil Red "O" staining respectively.SCR mRNA expression was examined with reverse transcription-polymerase chain reaction(RT-PCR).Results More uptake of Ox-LDL and Ac-LDL was observed in HMCL-SCR than that in the untransfected HMCL.Unlabeled Ox-LDL and Ac-LDL at a 40-fold excess concentration competed significantly with the uptake of Dil-Ac-LDL by HMC.Otherwise this phenomenon was not observed when co-cultured with LDL.Conclusion This results suggest that SR-A mediates the uptake of modified-LDL with high affinity and high specificity in human mesangial cells.

OBJECTIVE To strengthen the administrative work of infection control and to better the quality of medical care.METHODS To have a careful analysis of the problems relating to the infection management in grass-roots hospitals and to find the solution.RESULTS To establish an efficient administrative system and sound regulations,to regulate the relationship among the departments involved and to improve the working conditions so as to improve the quality of the infection management as well as to raise the level of medical care.CONCLUSIONS The infection management in grass-roots hospitals needs sound administrative setups and the personnel concerned should be clear about the responsibilities of the infection management.The staff′s awareness of infection prevention and control should be enhanced.The financial input should be increased,the basic facilities should be improved and upgraded,and the management,monitoring and supervision of the antibiotic should be reinforced.

This paper describes how the Institute of Materia Medica set up a special project considering the characteristics of pharmaceutical field,while conformed with the 'National Outline for Medium and Long Term S&T Development' and the Management of Special Research Fund at Central NonprofitResearchInstitutes.Takingtheresponsibilitiesofadministrationandproject implementation,the Institute explored measures in organizing,designing,implementation,and funding project and personnel stimulation.Especially,the Institute addressed the realization of project plan and expected objectives.After several years' experience,it was proved that the special fund is effective in advancement of personnel quality,technology,and the desciplines.It is also proposed that stable long-term support of special fund is essential for the Institute to develop as a united team.

Embedding invisible digital watermark in CT image means to use different kinds of algorithms to embed and store definite and confidential information in original CT image.The copy and internet transmission of the original data can be tracked even after decryption,so that the CT image data can be enciphered effectively.

Congenital heart disease is one of the most common birth defects.In early pregnancy,congenital heart disease screening methods mainly include neck translucency thickness measurement,ductus venosus flow measurement and fetal echocardiography.In the second trimester fetal heart disease most directly by fetal ultrasound examination for diagnosis of heartbeat,besides cardiac anatomic abnormalities,arrhythmia the diagnosis and assessment of heart function also are the important examination content.Focusing on more congenital heart disease related gene researches can provide reliable basis for prenatal diagnosis.Through the fetal congenital heart disease screening intervention,physicians can help early formulation of clinical decision making,alleviate the burden of the families,which has obvious social and economic benefits.

Objective:To study the reversal effect of tetrandrine on multidrug resistance of KBV200 cell line in order to provide experimental data for clinical individual chemotherapy.Methods:MTT reduction assay was used to investgate the inhibition of Adriamycin on KBV200cell line,and the reversal effects of Tetrandrine and Verapamil on the cell line.Results:Verapamil and Tetrandrine had the reveral effects on the cell line,and the reversal indexes of Tetrandrine and Veramil were 22.96 and 9.31,respectively.Conclusion:KBV200 cell line was multidrug resistance to Adriamycin but Tetrandrine had reversal effects on its multidrug resistance.

16.7mmol/L)48hours after injection.The successful diabetic rats models were randomly divided into diabetic mellitus(DM) group and taurine(TA) group.DM and TA groups were subdivided into DM1,DM2 and TA1,TA2 groups according to the duration of diabetes.The expression of Caspase-3 and NF-?B mRNA were studied by reverse-transcriptase polymerase chain reaction(RT-PCR) method.Results:NO obvious expression of Caspase-3 and NF-?B mRNA were found in the nomal groups compared with DM group after the 4th and 8th week.Under the intervention of taurine,the expression of Caspase-3 and NF-?B mRNA were inhibited compared with those in model.group(P

Objective:To explore the CT manifestations of intervertebral disc calcification(IDC)in children patients and its di-agnostic value in comparison with plain film.Methods:CT and plain film data of sixteen children with IDC were collected.The images were analyzed from the following aspects:counting the involved discs of each patient,locating the calcification in each ill disc and nearby tissue,observing the morphological features of calcification,and vertebral tube involvement.Results:①Fifteen patients had one or more than one cervical disc involved,but in only one patient,calcification involved five thoracic discs.Twenty-six discs were calcified in all,in which cervical disc accounted for 77%,and thoracic disc 23%.Most calcifications were nodular or patchy with different size.Sixteen calcification located at the anterior half segment of involved discs;Eight in the central part;only two at the posterior part.②CT and plain film detected calcification respectively of 26 and 25 discs.The for-mer was superior in displaying the location and appearance of calcification,and disclosing vertebral tube and nearby tissue in-volvement.Conclusion:CT is better than plain film in disclosing the details of ill disc.

Objective:To investigate the clinicopathological characteristics of extraskeletal myxoid chondrosarcoma(EMC).Methods:Nine cases of extraskeletal myxoid chondrosarcoma were studied.Immunohistochemical analysis was performed in all cases and ultrastructural studies were done in 2 extraskeletal myxoid chondrosarcomas.Follow-up information was available for seven patients.Results:There were 7 males and 2 females whose ages ranged from 31 to 69 years(median 52.78 years).Local pain or tenderness and the presence of a palpable mass were the main complaints of the patients.The tumors were located mainly in the lower extremities(66.7%).Most tumors were deep-seated.They usually had a distinct multinodular configuration delineated by fibrous connective tissue.The tumor cells were arranged in delicate intersecting strands,rings,and garlands for the most part.The myxoid matrix was abundant in most cases.Immunohistochemical analysis was performed in all cases and ultrastructural studies were done in 2 extraskeletal myxoid chondrosarcomas.EMC expressed vimentin(100%,9/9),neuron-specific enolase(77.8%,7/9),S-100 protein(66.7%,6/9),synaptop hysin and chromogranin A(22.2%,2/9),None of the tumors expressed EMA and desmin.Ultrastructurally,EMC was characterized by distinct cords of cells immersed in a glycosaminoglycan rich matrix.The cells were rich in mitochondria,had well-developed Golgi apparatus and there were numerous smooth vesicles.In many cells,there were also prominent glycogen deposits and lipid droplets.Some tumor cells had intracisternal microtubules.In one of 2 extraskeletal myxoid chondrosarcomas there were 140～180 nm diameter membrane-bound dense-core secretory granules in cell bodies.Conclusion:Extraskeletal myxoid chondrosarcoma is a rare soft tissue sarcoma characterized by distinctive morphological and cytogenetical features.It was believed to represent a variant of soft-tissue chondrosarcoma owing to its histological resemblance to chondroblastic tissue in the early stages of cartilage development or chondroid tumors such as skeletal chondrosarcoma.However,the chondroid nature has been a subject of controversy,and its line of differentiation remains to be determined.A substantial proportion of EMC show immunophenotypic and/or ultrastructural evidence of neuroendocrine differentiation.EMC has high potential of local recurrence and metastasis,and a high disease-associated death rate.