Five inner ears were removed from five rabbits under aneasthesia. The hard, thick bones of outer and middle ears were trimmed off, and the auditory ossicles were taken away. The cochleae were fixed with 2％ glutaraldehyde in phosphate buffer for eight hours, then they were decalcified in 10％ EDTA-2Na solution for ten days, and the softened shells of the cochleae were stripped off, and post-fixed in 1％ OsO_4 for an hour, dried at critical point, coated with gold, and observed under SEM.The rabbit cochlea shows two and a half turns, the modiolus is about 3.5mm high, and the organ of Corti lies on the basilar membrane of the spiral lamina. The outer hair cells usually form three parallel rows on the middle and basal turns but occasionally four rows on the top half turn. The inner hair cells form only a single row. Every bunch of the outer hairs consists of about 93-114 stereocilia which together show a "W" shape. Every bunch of inner hairs has about 50 cilia which together show an arch shape. The cilia on the top turn are longer than those on the base. The length and width of the inner and outer hair cells and four other kinds of cells were measured according to photos, and the numbers of these kinds of cells were estimated.Other three cochleae fixed at the time of 1, 2 or 4 hours after death were also observed. In the cochlea fixed four hours after death, the majority of the auditory hairs were found fallen down, which means the fixation of cochlea should be carried out within less than four hours after death.Some of the structural similarities and differences of cochleae among rabbit,human being and guinea pig were also compared in this paper.

Objectives: To investigate the effects of the extract mixture of alfalfa and perilla on lowering serum lipid, protecting liver and aortic wall in rats. Methods: Sixty SD rats were divided into six groups and were fed different diet. After seven or fourteen days, the rats were put to death and measured for serum lipids, cholesterol content of liver and aortic wall, and pathological change of the tissue. Results: There was a significant increase of serum TC, LDL-C, cholesterol content of liver and aortic wall in the high-fat fed rats. Under light microscope, there were lipid droplets and cholesterol crystal in liver of group high-fat. Compared with the group high-fat, the rats had a significant decrease of those measurements in serum or in tissue in the alfalfa and perilla group. Conclusions: The extract mixture of alfalfa and perilla could prevent the increase of serum TC, LDL-C, cholesterol content of liver and aortic wall in high-fat fed rats, and also prevent the fatty infiltration and cholesterol deposit of liver.

Neuroblastoma (NB)is a common malignant solid tumor in childhood,which has various clinical manifestations.It is difficult to diagnose and to prone to early metastasis.It is one of the main diseases causing death in childhood.The treatment of children with high-risk NB is still very challenging.At present,the treatments of NB mainly concentrated in the surgery,chemotherapy,radiation therapy and transplantation technology,etc.In recent years,more studies focus on targeted therapy and molecular biology (genetics) treatment.Targeted therapy is targeted to different specific loci of tumor,so as to kill tumor cells and reduce the impact on the normal tissue,which supplies more safe and effective treatments for children with NB.

Lipocalin-2 is an adipocytokine secreted mainly by adipose tissue. Increasing lines of evidence suggest that lipocalin-2 is an inflammatory factor associated with insulin resistence, obesity and its complications. The precise mechanism of the development of obesity-related disorders induced by lipocalin-2 is not very clear, however, it may be a useful target in drug therapy for obesity-related metabolic and cardiovascular diseases.

Synchronous colorectal liver metastases occurs in 15%-25% of patients with newly diagnosed colorectal cancer. Hepatic resoction has been accepted as the only option that offers long-term survival for patients with liver metastases. However, the optimal timing and sequence of resection for synchronous colorectal liver metastases still remain controversial. Also, the use of neoadjuvant chemotherapy which should be initiated in patients with resectable synchronous liver metastases is not consistent. In order to use evidence-based medicine to clarify indications for one-stage hepatic resection of liver metastases, a multi-center clinical trial was pedormed to normalize the surgical strategy of synchronous liver metastases. In summary, in selected patients, one-stage approach is comparable to the two-stage procedure in mortality and morbidity rates and longterm survival. One-stage procedure should be performed according to the site of primary tumor, age, the volume of residual healthy liver. Preoperative chemotherapy is effective in downstaging the tumor and reducing the resected liver volume, but it also brings damage to the liver and has adverse effect on onestage hepatic resection.

To promote the development of key discipline construction in hospitals,we described the integration of refined management into the construction of key disciplines and different aspects including personnel training,featured specialty,research,and hardware were discussed.We concluded that refined management in essential to the development of hospital,and a mechanism needs to be established to ensure the long-term effect.

Objective To investigate the diagnosis and treatment of intestinal vessel malformation(IVMF). Methods A retrospectively studiy on the clinical data of 21 cases of IVMF was made. Results The clinical characterstics of IVMF were long course and hemorrhage. The hemorrhage was intermittent and self-limited, which mostly showed black stool or positive in occult test. Of 15 examined by angiography, pathological changes were found in 12 coses and 1 was suspected. All 21 cases underwent operations, of them,14 cases were found pathological changes by touch, diaphany and methylene blue injection,while the other 7 cases were confirmed by endoscopy during operation. Conclusion Angiography is an effective method. During the operation,diaphany must be regarded as a routine examination,methylene blue injection is simple and effective,and endoscopy is also very important.

Objective To determine the incidence of long bone lesions and clinical characteristic in early newborn with asymptomatic congenital syphilis. Methods The syphilis antibody in 26 infants with asymptomatic congenital syphilis who were diagnosed and treated in our hospital from April 2001 to May 2004 were detected,and the clinical feature and X-ray features were analyzed.Results Twenty-six infants and their mothers all had the antibody positive of syphilis.Treponemal antibody titer were 4 times higher than their mothers in the infants blood serum.Their general conditions showed normal and had no symptoms when they were hospita-(lized).Then,there were 24 cases (92%) with positive physical signs and /or abnormal findings on the liver function tests and blood routine and long-bone radiographs.Long-bone radiographs were abnormal for approximately 38%(10 of 26)of asymptomatic newborns with congenital syphilis.Metaphysitis was shown and looked like sandwich biscuit in X-ray photograph.Conclusions Asymptomatic congenital syphilis has no symptoms in the early stage.Bone X-ray photograph should be included in the assessment of newborns with a positive serology.Further support is needed in the diagnosis and treatment of early newborns with asymptomatic congenital syphilis.

Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia worldwide with a trend of increasing morbidity and prevalence. It usually links to a high disability and mortality rate. Stroke is the most important and severe complication of AF, as a core strategy to prevent it, anticoagulation therapy is recommended to reduce the risk of stroke events. However, management risks including poor treatment adherence, individual difference and bleeding risk can′t be ignored. In the western countries, they have successively established a nurse-led integrated chronic care program for patients with atrial fibrillation. We can use it to guide our future work. Anticoagulation therapy is a long-term managing process. To integrate the existing medical resources, nurses must give their full play to access the deep needs and guide them to learn the core skills of management, thus improving the adherence to anticoagulation therapy.

Objective To observe and analyze curative efficacy of Desloratadine Citrate Disodium in combination with endoscopic sinus surgery and its effects on serum TIgE, ECP and inlfammatory factors in patients with chronic sinusitis.Methods 90 patients with chronic sinusitis who received therapy in our hospital from January 2015 to June 2016 were collected as research objects to be randomly divided into the control group and the observation group equally. The control group was given endoscopic sinus surgery, and the observation group was given Desloratadine Citrate Disodium in combination with endoscopic sinus surgery. Then, visual analogue scale (VAS) was applied to assess the severity of subjective symptoms of the disease. Three months after the surgery, the curative efifcacy, scores of visual analogue scale (VAS) relfecting clinical symptoms, levels of serum TIgE, ECP and inlfammatory factors were compared between the two groups.Results All patients undergone successful surgery. The observation group had a total therapeutic efifciency ratio of 95.6%, which was signiifcantly higher than that of 82.2% in the control group (P < 0.05). In comparison with the control group, the observation group had lower VAS scores of nasal discharge, nasal obstruction, cheek pain, headache, hyposmia [(1.84 ± 0.89) vs (3.12 ± 1.03) points, (1.87 ± 0.81) vs (2.55 ± 1.14) points, (1.24 ± 0.86) vs (1.88 ± 0.79) points, (1.15 ± 0.54) vs (1.97 ± 0.83) points, (1.84 ± 0.73) vs (2.18 ± 0.84) points], lower levels of serum TIgE, ECP [(58.62 ± 7.36) vs (64.39 ± 7.18) kU/L, (4.25 ± 0.84) vs (5.01 ± 1.07) ng/L)] and inflammatory factors of TNF-α, IL-6, IL-8 [(1.27 ± 0.46) vs (2.62 ± 0.53) ng/ml, (5.13 ± 1.24) vs (9.54 ± 2.23) pg/ml, (282.63 ± 32.42) vs (315.84 ± 35.89) ng/L)] with statistical differences (allP < 0.05).Conclusion Desloratadine Citrate Disodium in combination with endoscopic sinus surgery is effective for chronic sinusitis, which can signiifcantly relieve clinical symptoms, reduce levels of serum TIgE, ECP and alleviate inlfammatory reaction.