Objective:To observe the effect of prolonging life and inhibiting tumor growth of Xiaozhensan on H22 cells-bearing mice and study the mechanism of action. Methods:①H22 cells-bearing mice models were made by subcutaneous injection and were divided into six groups:model group,low,middle and high dosage groups of Xiaozhensan,5-FU group,5-FU plus Xiaozhensan middle dosage group. After 10 days' treatment,the mice were killed and the anti-tumor rates were calculated,to detect the content of IL-2 and observe pathological sections of tumor and expression of Bcl-2 by immunohistochemical method. ②H22 cells-bearing ascites mice models were divided as the above. The living days of the mice were recorded,the prolongation of life span were calculated. Results:Xiaozhensan had the function of inhibiting the growth of tumor,and there was the dependability between quantity and effect. In pathomorphology,the sections of Xiaozhensan groups showed nuclear shrinkage,necrosis,hemorrhage,inflammatory cell infiltration on the periphery,etc. Xiaozhensan could increase the content of IL-2 and inhibit the expression of Bcl-2. Xiaozhensan combined with 5-FU can prolong the life span of the mice bearing ascites tumor. Conclusion:Xiaozhensan had the function of inhibiting the growth of tumor. The mechanism of action may be concerned with these aspects:enhancing the cellullar immunologic response and strengthening the ability to resist tumor,inducing apoptosis and weakening reproductive activity of tumor,etc. Middle dosage group of Xiao Zhen San combined with anticarcinogenic western medicin can prolong the life span of the mice bearing ascites tumor. This study provided dependable experimental documents for clinical application of Xiaozhensan,and had some guiding significance in clinic.

[Objective]This paper mainly introduced Professor YE Jin 's experience in treating girls with precocious puberty from liver syndrome differentiation and characteristics of prescription medication.[Methods] On account of three main symptoms of girls with precocious puberty at first visit, including thelarche, leukorrhea and menses, we explore the relation between liver and the causes of this disease from the viscera-state doctrine as well as the meridian theory. Then introduce Professor YE Jin's methods and empirical medication in treating girls with precocious puberty based on liver. In order to analyse her experience, a clinical case was reported. [Results]Professor YE holds that the pathogenesis of girl 's precociouss puberty closely related to liver. The deficiency of liver-yin and kidney-yin is the basic pathogenesis of precocious puberty, which is often accompanied with liver qi depression or even liver qi depression transforming into fire. On the basis of nourishing yin and purging fire, Professor YE emphasizes treating precocious puberty from liver. In practical terms, flexibly applying the ideas of softening, soothing and clearing liver can effectively improve the clinical symptoms. She makes the selection and compatibility of Chinese medicine comforming to the characteristics of children 's physiology and pathology and values the protection of vital energy. The reported case specifically demonstrates treating a girl 's thelarche from liver syndrome differentiation as well as the empiric therapy. [Conclusion]Professor YE Jin's experience in treating girls with precocious puberty is well worth learning for its significant curative effect.

Objective To determine the effects of down-regulating serine/threonine/tyrosine kinase 1 (STYK1) on the proliferation,apoptosis and invasion and on MEK/ERK and PI3K/AKT signaling pathways in human colorectal cancer cell line HCT-15.Methods The expression of STYK1 protein was downregulated by RNA interference technique in HCT-15 cells.Then,the STYK1 protein expression was detected by Western blotting.CCK-8 assay was used to measure the effect of STYK1 down-regulation on the proliferation of the cells,flow cytometry was employed to detect the cell apoptosis,and Transwell chamber assay was adopted for cell invasion ability.The expression levels of p-ERK1/2 and p-AKT were detected by Western blotting.Results Compared with the siRNA NC group,STYK1 down-regulation significantly inhibited the proliferation (P ＜ 0.01) and invasion (P ＜ 0.01),induced cell apoptosis (P ＜ 0.01),and suppressed the expression levels of p-ERK1/2 and p-AKT in the HCT cells transfected with STYK1 siRNA (P ＜0.01).Conclusion STYK1 down-regulation inhibits the proliferation and invasion and induces the apoptosis in colorectal cancer cells,which may be related to its inhibition on the MEK/ERK and PI3K/AKT signaling pathways.

Objective To discuss clinical significance of the neutrophil alkaline phosphatase(NAP) activity in polycythemia pa‐tients .Methods 35 cases of patients with secondary polycythemia(secondary group) and 22 patients with polycythemia vera (poly‐cythemia vera group) were enrolled in the study ,whose peripheral blood smears were stained by using chemical method and the pos‐itive rate and scores of NAP were assessed .In addition to that ,30 cases of healthy people who had underwent physical examination were recruited as control group .Results NAP positive rate and scores of the polycythemia vera group were significantly higher than those of secondary and control group(P<0 .05) ,while no statistical difference was observed between secondary and control group(P>0 .05) .Conclusion NAP score is a good methord to diagnose polycythemia ,which could facilitate rapid ,accurate diagno‐sis and be used as auxiliary indicators in the differential diagnosis of polycythemia .

[abstract] Objective To study the health education on diabetes nurse diabetes management mode at the core of application in the patients with follow-up effect. Methods Our hospital endocrinology in October 2015,participate in blood sugar management company research project(namely,doctors,nurses and patients ofthree Eonline and offline (O2O) integration management system).The project is the specialist education nurses,doctors and patients of organic whole,to form a closed-loop management.Use of the project with mobile tablet (PAD),SMS platform on the first base into a group of patients with type 2 diabetes insulin for 5 minutes on education and baseline data collection,and in the next 15 weeks of diabetes health education nurses via text message and phone tracking in the form of blood sugar and insulin dose data,at the same time for more than three months of education,guidance and management,to help patients with initial foundation of insulin self management knowledge and skills,promote the blood glucose control is at target. Results In 12 weeks of the completion of this project and follow-up,425 patients of FPG success rate is 82.6%. Conclusion Nurse diabetes health education as the core of diabetes management model is feasible in patients with type 2 diabetes, the effect is good,that greatly arouse the subjective initiative of patients;Improve the patients′self-management ability,improving according to the medical behavior;Improved treatment adherence to standard prompt patients FPG.

OBJECTIVE:To observe the protective effect of the traditional Chinese medicine Yiqimingmu oral solution on photochemical damage of retina in rats. METHODS:SD rats were divided into four groups randomly:negative control group, model group, low dose Yiqimingmu oral solution group and high dose Yiqimingmu oral solution group. All the groups except the control one were continually exposed to Lux (1 900?106.9) green fluorescent light for 24 hours to establish retinal photochemical damage model. The low dose and high dose groups were administered intragastrically with Yiqimingmu oral solution (15mL?kg-1?d-1 and 30mL?kg-1?d-1) 7 days before light expose. Normal saline solution was administered intragastrically in control and model group. At 6 hours and 6, 14 days after light expose, the content of malandialdehyde (MDA) and activity of SOD in rats' retina were measured. The histopathologic changes were observed by transmission electron microscopy and light microscopy. RESULTS:At 6 and 14 days after light expose, the activity of SOD in Yiqimingmu solution high dose group were higher than in the model control group(P

A girl who aged eight years and seven months presented with prunosus patches on the right buttock for 8 years,gradual unilateral enlargement of the right lower limb for more than 7 years,and multiple vegetations for 1 year.Dermatological examination showed nevus flammeus and multiple malodorous vegetations over the right lower limb with high skin temperature.The right lower limb was thicker and longer than the left lower limb.X-ray examination,magnetic resonance imaging and Doppler ultrasound examination revealed high-flow vascular malformations.Pathological examination of the vegetations showed vascular proliferation,fibroblast proliferation and erythrocyte extravasation.She was diagnosed as Parkes-Weber syndrome accompanied by pseudo-Kaposi's sarcoma.

Objective To observe the results of function MRI and perimetry in patients with visual pathway diseases. Methods Three patients (6 eyes) with pituitary adenoma and craniopharyngioma diagnosed via pathological examination and three healthy volunteers aged from 24 to 30 were collected. The best corrected visual acuity was non-light perception-1. 0 in the 6 sick eyes and 1. 0 in the healthy eyes;all the involved individuals had no other ocular diseases except myopia and without any contraindications of MRI. Common tests including the best visual acuity, fundus test by direct or indirect ophthalmoscope, center static visual field tested by Octopus 101 perimeter, program 32, tendency oriented perimetry were performed. The visual stimulation subtended a field of view of about 12 degrees,consisted of high contrast and drifting checkerboards. MRI parameters: GE signa VH/i 3. 0T scanner. Functional data: GRE-EPI sequence, 20 slices lying perpendicular to the calcarine sulcus. Anatomical data was obtained using 3DSPGR sequence to acquire high resolution. The cortical surface was unfolded and then cut and inflated. Functional data was presented to the inflated surface and subsequently analyzed by AFNI software. Results In six eyes, three had temporal defects, two had upper temporal visual field defects, and the other one did not finish the visual field test. The retinotopic representations of health adults were obtained by using the phase-encoded visual stimulation. The Eccentricity coordinate maps showed that foveal representations lay in the occipital poles and the representations appeared further anterior as eccentricity increased. The polar angle coordinate maps showed that early retinotopically organized areas had a representation of visual field. The visual cortex beneath the calcarine sulcus matched with the upper visual field of the opposite side and which upon the calcarine sulcus matched with the under visual field of the opposite side. Less or no visual cortex response was revealed in the patients' function MRI or the response in injury side was vanished. The visual cortex response related with the visual field defects could not be induced in function MRI. Conclusion There is a good correlation between function MRI data and the results of perimetric evaluation. The function MRI can show the visual cortex response correlated with the visual field defects of the patients with visual pathway diseases.

Objective To observe the clinical efficacy of fire-needle therapy plus oral administration ofGuizhi Fuling capsules in treating ovarian cysts.Method Seventy-six patients with ovarian cysts were randomized into a treatment group and a control group, 38 cases each. The treatment group was intervened by fire-needle therapy plus oral administration ofGuizhi Fuling capsules, while the control group was intervened by oral administration ofGuizhi Fuling capsules alone. Before and after the treatment, ultrasound B-scanning was adopted to measure the sizes of ovarian cysts, and the therapeutic efficacies were compared between the two groups.Result The sizes of ovarian cysts were significantly changed after the treatment in both groups (P<0.01). The sizes of ovarian cysts in the treatment group were significantly different from those in the control group after the intervention (P<0.05). The total effective rate was 86.8% in the treatment group versus 65.8% in the control group, and the between-group difference was statistically significant (P<0.05).Conclusion Fire acupuncture plus oral administration ofGuizhi Fuling capsules is an effective approach in treating ovarian cysts.

AIM: To comprehensively investigate the current status of cataract surgery in Leshan city, and find the existing problems according to the survey results as a guide to cataract prevention and treatment in future.
METHODS: We surveyed the status of cataract surgery containing equipments, surgeons and surgery conditions in 17 ophthalmology departments of general hospitals from Leshan including 6 counties, 1 county-level city and 4 districts during 2012. Cataract surgery methods, according to various surgery financial resources, surgical performances and surgical incision, were divided into phacoemulsification and intraocular lens implantation ( Phaco + IOL ) , extracapsular cataract extraction and intraocular lens implantation ( ECCE + IOL ) , and each method contained 2 groups. We analyzed the outcomes following preoperative examination, surgeons, surgical methods, surgical equipments, types of IOL, surgical quantity, surgical results, and intraoperative complication.
RESULTS: In whole city there were 16 operating microscopes, 43 slit lamp microscopes, 12 non-contact tonometers, 1 intraocular lens Master, 8 optical A/B type ultrasonic examination systems, 4 YAG lasers and 12 phacoemulsification instruments. There 15 doctors could complete cataract surgery independently, and 5 of them were phacoemulsification surgeons. The total number of completed cataract surgeries reached 6 211 eyes, containing 3 564 eyes for Phaco+IOL ( 57%) ( preoperative visual acuity ≥0. 3 in Phaco+IOL1 were 1 520 eyes, 24%) and 2 647 eyes for ECCE+IOL ( 43%) ( 1 533 eyes in ECCE+IOL1, 25%). In various groups, off-blindness rate was 94. 80%-100%, off-disability rate was 90. 41%-100%, and 94. 96% - 100% received intraocular lens implantation, intraoperative complication rate was 1. 00%-15. 10%.
CONCLUSION: The distribution of surgeons and equipments in the whole city is unbalanced, and the utilization rate of phaco equipment is low. The levels are different in various hospitals on surgical technique, surgical quantity and surgical results. Phacoemulsification and intraocular lens implantation is considered as the main therapy for cataract, and as a transition to dioptric and personalized cataract surgery. The surgical condition of the city is good, but the proportion of cataract surgery for prevention of blindness in Leshan city is low, and the surgical technique remains to be further improved.