Air conditioning system should provide a continuous and stable production environment for drug production and meet the dynamic needs of drug production process. Through special compliance modifications of clean air conditioning system, the operation stability of clean environment can be improved, the temperature and humidity control in clean area in winter can be optimized, the risk of pollution and cross contamination in production process can be reduced, and the clean environment can be ensured to meet the current GMP and WHO compliance requirements. The vaccine filling workshop to be renovated this time in Changchun Institute of Biological Products was built earlier. Although it has been partially renovated and adjusted, many risks related to air conditioning system were found. Therefore, combining the concept of Quality by Design(QbD), this paper applies risk analysis tools to design the air conditioning system compliance renovation scheme of the workshop, throughout the entire process of feasibility study, renovation implementation, system debugging and verification of technical solutions, so as to improve the compliance and reliability of clean environment and the operation stability of clean air conditioning system in the renovated workshop, solve the industry problem of winter cooling in vaccine workshops in northern region, and reduce the investment cost of project renovation and construction, as well as the operation and maintenance cost of clean air conditioning system.

Small ubiquitin-like modifiers (SUMO) are a family of proteins that modulate important functional properties,including protein interaction,subcellular localization,protein dimerization,DNA binding and/or transactivation of transcription factors.It has been suggested that SUMO proteins may play an important role in breast carcinogenesis by sumoylation of estrogen signaling proteins such as co-regulators,and breast cancer-related proteins.

The application of genetic engineering technology in modern agriculture shows its outstanding role in dealing with food shortage. Traditional medicinal plant cultivation and collection have also faced with challenges, such as lack of resources, deterioration of environment, germplasm of recession and a series of problems. Genetic engineering can be used to improve the disease resistance, insect resistance, herbicides resistant ability of medicinal plant, also can improve the medicinal plant yield and increase the content of active substances in medicinal plants. Thus, the potent biotechnology can play an important role in protection and large area planting of medicinal plants. In the development of medicinal plant genetic engineering, the safety of transgenic medicinal plants should also be paid attention to. A set of scientific safety evaluation and judgment standard which is suitable for transgenic medicinal plants should be established based on the recognition of the particularity of medicinal plants.
Genetic Engineering ; Plant Diseases ; genetics ; prevention & control ; Plants, Genetically Modified ; chemistry ; genetics ; growth & development ; metabolism ; Plants, Medicinal ; chemistry ; genetics ; growth & development ; metabolism

Objective To investigate the mechanism of glycoprotein serglycin (SRGN) promoting metastasis of breast cancer cells and the possible mechanism of SRGN expression.Methods Real time quantitative polymerase chain reaction (PCR) and bioinformation retrieval were used to detect the expression of SRGN in lymph node metastasis and non-metastasis breast cancer.MDA-MB-231 shRNA and MCF-7-SRGN of breast cancer stable cell line were established by lentivirus shRNA interferencc and overexpression.Transwell assay was used to test the effect of SRGN on invasion and metastasis of breast cancer cell line in vitro.Western blot assay was used to detect the changes of epithelial-mesenchymal (EMT) related markers.The possible regulatory mechanism of SRGN expression was detected by Western blot assay.Results SRGN expression was significantly increased in lymph node metastasis of breast cancer in clinical specimens.SRGN interference inhibited the invasion and metastasis of tumor cells.SRGN promoted breast cancer cells EMT.Transforming growth factor β1 (TGFβ1) promoted the expression of beta SRGN transcription.Conclusions SRGN can induce the change of EMT in breast cancer cells and promote the invasion and metastasis of breast cancer cells.

Objective To explore the characteristics and laws of burned children,and to find the coping strategies on preventing and reducing the incidence of pediatric burns.Methods The age distribution,burn causes, degree of burn,easy -happening months and on -site treatment of 978 children under 12 years old were retrospective-ly analyzed.Results 978 pediatric burns patients made up 55.4% of all burned patients among which there were 601 male patients and 377 female patients.Most of them were 1 -3 years old.The main reason of burn was hot liquid. Most of them were minor and moderate burned.The easy -happing months were from May to September.Only 11.0%of these children were correctly treated.Most burn children were from countryside.Conclusion The main reasons of children being burned are poor management of hot liquid,carelessness and the indifference of safety consciousness. The cases of burned children are regular.It is important to strengthen the publicity and education of precaution and the consciousness of safety protection of custodian.The publicity of knowledge about burning safety and correct on -site treatment are important for prevention and cure of burned children.

Adolescent ; Arthritis, Juvenile ; blood ; immunology ; pathology ; Child ; Child, Preschool ; Female ; Humans ; Immunoglobulin A ; blood ; Immunoglobulin G ; blood ; Immunoglobulin M ; blood ; Interferon-gamma ; blood ; Interleukin-4 ; blood ; Male

OBJECTIVE To discuss the management experience of protective measures for emergently admitted influenza H1N1 patients in our hospital who were converted from general ward into isolated ward in no more than three hours.METHODS Laying out the guideline of the work,personal training and personal protective measures were taken to manage the staff who working in the isolated ward.RESULTS There was statistically significant difference in hand washing,wearing mask and head wear,taken off gloves,mask and clothes among medical staff who worked in the isolated ward,in comparison with staff from other ward and non-medical staff From 14 points of suruey.there were 8 points were over 80% pass rate.The use of protective equipment was correct.without any cross infection.CONCLUSIONS Management of protective measures plays an important role in control of cross infection in treatment of H1N1 patients.

Objective To investigate the etiology and prognostic factors of acute kidney injury (AKI) in pediatric patients . Methods The clinical data of children patients with AKI in this hospital ,including the clinical features ,causes and outcomes ,were retrospectively analyzed .The relationship between the risk factors and prognosis was analyzed .Results Infants were dominated by the prerenal factors and the other age groups were dominated by the renal factors .The univariate analysis revealed that the AKI stage ,etiology ,mechanical ventilation ,sepsis/septic shock ,MODS ,acidosis ,creatinine initial value ,creatinine peak value and serum potassium were the factors affecting prognosis .The Logistic regression analysis showed that mechanical ventilation ,MODS and sep-sis/septic shock were the independent risk factors affecting prognosis .Conclusion The etiology of AKI in children is diverse and its distribution has the age characteristics .Mechanical ventilation ,MODS and sepsis/septic shock are the independent risk factors af-fecting prognosis .The early diagnosis and the active treatment conduces to improve prognosis .

0.05.Conclusion Using XT-2000i Automated Hematology Analyzer to test the reticulocyte the reproducibility is better,and the linearity can content well with clinic,the rate of carry contamination is low,the stability is better too.Compared XT-2000i with the manual methods,the relativity is better. It can replace the manual methods.

Staphylococcus epidermidis is a commensal bacteria which inhabits on the surface of human skin and mucous membrane.It has been demonstrated that staphylococcus epidermidis is a major opportunistic pathogen that can cause clinical biomaterial related infection and plays an important role in biomaterial implantation associated infections.The main pathogenic factor is the formation of the bacterial biofilm on surface of medical biomaterial.The formation of bacterial biofilm can resist the defense reaction and antibiotic treatment effectively so that leading to the biomaterial implantation associated infection which is difficult to cure thoroughly,and make the infections become chronic,persistent and repetitive.Nowadays,the infections caused by Staphylococcus epidermidis have caused high clinical mortality.Staphylococcus epidermidis biofilm formation,the regulations of intercellular adhesion gene (ica) operon and accessory gene regulator (agr) gene on the formation of biofilm and their functions in clinical biomaterial related infections are reviewed in this article.