The formation of Staphylococcus epidermidis biofilm on the surface of medical biomaterials may resist the antibiotics treatment and cause chronic infection,which has become a research focus in recent years.Multiple genes constitute complex regulatory network which affect the biofilm formation,and play different roles in the different stages of biofilm formation.Accessory gene regulator (agr) is one of the most important genes in the process of biofilm formation.The process of bacterial biofilm formation,research status of regulation mechanism of agr system and its related genes in the formation of biofilm are reviewed,to provide reference of using agr as a target for the treatment of staphylococcus epidermidis biofilm related infections.

Lycopene—a kind of important active compound of caroteinoids, is greatly beneficial to human health with its diverse biological functions. With the elucidation of lycopene biosynthetic pathway and cloning genes of relative enzymes from microorganisms, it is possible to regulate lycopene biosynthesis via genetic engineering. The biosynthesis pathways of lycopene and gene cloning of lycopene biosynthetic enzymes in microorganisms were reviewed, and gene engineering strains documented in previous works including: E.coli and yeast constructed by genetic recombination, mold strains enhanced the ability of producing lycopene by gene manipulation were summarized. At last, compared with the present methods, the problems existed in the process of construction were pointed out.

Aim To study the effects of preconditioning with desflurane, sevoflurane and isoflurane on adenosine triphosphate (ATP) in anoxia-reoxygenation myocardial cells. Methods Rat ventricular myocytes, cultured for 4～5 days, were randomly allocated to five groups: Control group, anoxia-reoxygenation group and groups preconditioned with 1.5 MAC desflurane, sevoflurane or isoflurane following anoxia-reoxygenation. The content of intracellular ATP ,the activities of lactic dehydrogenase(LDH) and creatine kinase(CK), and the cell viability were measured at the end of experiment.Results Preconditioning with 1.5 MAC desfllurane, sevoflurane or isoflurane significantly attenuated the great reduction in ATP and cell viability and the increase of LDH and CK caused by anoxia-reoxygenation. There was a positive correlationship between ATP and cell viability,and a negatiue correlationship between LDH and CK (r was 0.83, -0.87 and -0.82 respectively, P

Adult ; Carcinosarcoma ; pathology ; Female ; Heart Neoplasms ; pathology ; Humans ; Lung Neoplasms ; pathology ; Myocardium ; pathology

RNAi is a process of effective gene silencing. Human papillomaviruses 16 (HPV16), as the most prevalent genotype of cervical cancer, encodes the E6 and E7 oncogenes, which is pivotal for maintenance of malignant phenotypes. Recently, some significant advances have been obtained in RNA interfering effect on HPV16 E6/E7. In vitro, it has been developed more effective vector systems and screened for more specific targeted sequences. In vivo, the corresponding animal models have been established, which lay a good foundation for the clinical research.

Objective To OxplorO thO rOlationship bOtwOOn diffOrOnt vOntilation modO and hypocapnia in prOtOrm infants.Methods From April 2014 to OctobOr 2017,a total of 302 prOtOrm infants with rOspiratory distrOss syndromO in Suining COntral Hospital who rOcOivOd diffOrOnt assistOd vOntilation wOrO sOlOctOd in this rOsOarch. According diffOrOnt vOntilation modO, thOy wOrO dividOd into convOntional mOchanical vOntilation(CMV) group( n=66),thO mOchanical vOntilation modO was CMV, continuous positivO airway prOssurO(CPAP) group(n=149),thO mOchanical vOntilation modO was CPAP, high frOquOncy vOntilation(HFOV) group(n=87),thO mOchanical vOntila-tion modO was HFOV. ThO morbidity of hypocapnia in diffOrOnt groups and prOvOntion mOthods wOrO analyzOd. Results Among 302 childrOn,52casOs had hypocarbonOmia,thO total morbidity of hypocapnia was 17.2%. ThO morbidity of hypocapnia in thO HFOV group was 37.9% (33/87),which in thO CMV group was 21.2% (14/66), which in thO CPAP group was 3.4% (5/149),thO diffOrOncO among thO thrOO groups was statistically significant(χ2 =47.013,P=0.000).Conclusion ThO incidOncO of invasivO vOntilation should bO carOfully considOrOd in prOtOrm infants, OspOcially high frOquOncy vOntilation. To prOvOnt and trOatmOnt of hypocapnia, timOly adjustmOnt of vOntilator paramOtOrs according to blood gas during diffOrOnt vOntilation modO should bO donO.

OBJECTIVE: To observe the correlation between the threshold of tone burst auditory brain stem response (Tb-ABR) and ASSR (auditory steady-state response) and the threshold of BA (behavioral audiometry). To investigate the accuracy and clinical value of Tb-ABR and ASSR in pediatric hearing assessment. METHOD: From January, 2014 to December, 2014, 76 children (123 ears) recieved hearing examination in the First Affiliated Hospital of Zhengzhou University. They were classified into three groups according to the hearing level: 23 children (46 ears) with normal hearing ability in group A, 27 children (32 ears) with slightly-moderate sensorineural deafness in group B and 26 children (45 ears) with severe-profound sensorineural deafness in group C. Tb-ABR, ASSR, BA in 4 frequencies (0.5, 1.0, 2.0, 4.0 kHz) were tested and the results were statistically analyzed. RESULT: (1) At the 4 frequencies (0.5-4.0 kHz), we obtained 132 thresholds of Tb-ABR, 144 of ASSR, 152of BA. And 166 thresholds were obtained in Tb-ABR+ASSR+BA in total . (2) The thresholds of Tb-ABR, ASSR and those of BA in all 3 groups had linear relations at 0.5-4.0 kHz. The correlation coefficients of group A were 0.76, 0.82, 0.87 and 0.91; 0.52, 0.57, 0.67 and 0.64. Those of Group B were 0.89, 0.95, 0.98 and 0.95; 0.74, 0.82, 0.87 and 0.90. Those of Group C were 0.91, 0.90, 0.92 and 0.89; 0.93, 0.95, 0.95 and 0.91. CONCLUSION (1) Both ASSR and Tb-ABR can allow reasonably accurate predictions for the pediatric hearing assessment. The correlation between Tb-ABR threshold and BA threshold is higher in normal hearing children and slightly-moderate sensorineural deafness children. The correlation between ASSR threshold and BA threshold is higher in severe-profound sensorineural deafness children. (2) The hearing test combination can evaluate the residual hearing ability for children with severe hearing loss.
Audiometry ; Auditory Threshold ; Child ; Evoked Potentials, Auditory, Brain Stem ; Hearing ; Hearing Loss, Sensorineural ; diagnosis ; Hearing Tests ; Humans

Objective To explore the impact of gratitude on interpersonal disturbance in college students and provide positive energy into the establishment of harmonious campus interpersonal relationship.Methods A total of 530 college students at 5 uni-versities in Guangdong province were surveyed by gratitude questionnaires and comprehensive diagnosis scale of interpersonal rela-tions.Results (1)The rate of interpersonal disturbance in college students was 46.4%.(2)Gratitude interacted with genders and grades(P <0.05 ).The levels of gratitude in male freshmen,sophomores,and seniors were remarkably higher than those in male juniors(P <0.01),and female juniors had a higher level of gratitude than their male counterparts remarkably(P <0.01).Scores of male students were significantly higher than those in female students in interpersonal communication,way of treating people,oppo-site-gender interaction and the total scores of interpersonal disturbance(P <0.01 ).Besides,juniors had a remarkably higher score than freshmen and seniors in the factor of social association and friend making (P <0.05 ).(3)There was a significantly negative correlation between the level of gratitude in college students and the total scores of interpersonal disturbance as well as the four fac-tors of interpersonal communication,social association and friend making,way of treating people,and opposite-gender interaction (P <0.01).Subjects with different levels of gratitude showed marked variation in the total scores of interpersonal disturbance and all its factors(P <0.01).Gratitude displayed a significantly negative predictive effect on the total scores of interpersonal disturbance and all its factors(P <0.01).Conclusion Close relation existed between gratitude of college students and their interpersonal dis-turbance,and gratitude could be used to effectively predict college students′interpersonal disturbance.

Objective To explore the angioarchitectrue characteristics and appropriate treatment strategies of dural arteriovenous fistula (DAVF) in foramen magnum region.Methods The clinical data of patients with DAVF diagnosed by digital subtraction angiography (DSA) were analyzed retrospectively.Results Thirteen patients intraoperative were found fistula and complete resection,patients discharged from hospital,DSA check showed that the DAVF fistula completely disappeared,all patients symptoms were improved to different extents.Conclusions The clinical features,prognosis and treatment methods of DAVF in forament magnum region depends on its angioarchitecture,especially the lesion site and venous drainage,surgical operation can usually find the fistula and can be completely removed.

Objective Geriatric medicine has become an important medical subspecialty,and will have broad prospects for development.By reviewing the brief history of geriatric medicine,it displays its emergence,birth,concept and scope under different background of social civilization and culture between western and eastern countries.Moreover,it presents the development courses of geriatric medicine in terms of clinical medicine,nursing,education,disciplinary development,academic organization,management and innovation among different countries.As the world's population ages,geriatric medicine is facing both great challenges and opportunities.Only by learning the knowledge and experience from the history and renewing ideas,healthcare professionals can promote the comprehensive development of geriatric medicine,which will be full of vigor and vitality.