Apoptosis ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Flavonoids ; pharmacology ; Humans ; Isoflavones ; chemistry ; Leukemia, Promyelocytic, Acute ; pathology ; Tretinoin ; pharmacology

Objective To explore the clinical significance of combined detection of carbohydrate antigen(CA)125,CA153,carcinoembryonic antigen(CEA) and tumor specific growth factor(TSGF) in diagnosis for breast cancer.Methods A total of 125 patients with breast cancer were recruited as objects in this study from march 2015 to march 2016,65 patients in breast cancer group,60 patients in benign breast disease group,meanwhile 55 healthy person were enrolled in the control group.Serum tumor markers such as CA125,CA153,CEA and TSGF were detected and compared in the three groups.Results The serum CA125,CA153,CEA and TSGF levels in the breast cancer group were significant higher than those of benign breast disease group and healthy group,the differences were statistical significant(P<0.05).At the same time,the sensitivity and specificity of joint detection of four kinds of serum tumor marker were 90.2% and 88.9%,which were higher than those of single serum tumor marker detection(χ2=26.12,P<0.05).Conclusion The four kinds of serum tumor markers combined testing not only increases the sensitivity of breast cancer diagnosis,but also improved the specificity of diagnosis of breast cancer.

Objective To explore the risk factors affecting the quality of life (QOL) of patients with obstructive sleep apnea (OSAS) and the influence of nasal continual positive airway pressure (nCPAP) treatment on the QOL of OSAS patients. Methods Measuring the quality of life of 212 patients suffered from OSAS with WHOQOL-BREF and analyzing the influences of the sleepiness, quality of sleep, chronic disease and nCPAP treatment on the QOL of patients with OSAS. Results The scores on physical domain and psychological domain of patients who were sleepiness or had bad quality of sleep were less than that of patients who were non-sleepiness or had a good sleep respectively(P

Objective To compare the treatment accomplishmentsand adverse effects of the mixed- or single-application of etomidate and propofol during modified electroconvulsive therapy (MECT) in schizophrenics with hypertension. Methods Ninety hospitalized schizophrenics with hypertension undergoing MECT were ran-domly assigned to 3 groups: Group EP(etomidate = 0.3 mg/kg and propofol = 1.0 mg/kg), Group E (etomi-date = 0.7 mg/kg) and Group P (propofol = 2.0 mg/kg). Changes of SBP, DBP and HR were recorded before anesthesia, after induction, electrical stimulation instantly and 5 min after electrical stimulation. The average pe-riods of epileptic seizure (ESD), postictal suppression index (PSI) and the adverse reactions were also recorded. Results The ESD and PSI scores were significantly higher in Group EP and Group E than those in Group P (P < 0.05). SBP and DBP in Group E were significantly higher at the electrical stimulation instant than those af-ter induction (P < 0.05). SBP and DBP in Group P were significantly lower after induction than those before anesthesia(P < 0.05). The injection pain of Group EP and Group E were lower than that of Group P(P < 0.05). No significant differences were found among 3 groups in the adverse reactions such as nausea/vomiting anddys-phoria.Conclusion The mixed liquor of etomidate and propofol can extend the time of seizure, reduce the nega-tive reaction of cardiovascular system and adverse reactions during MECT in schizophrenics with hypertension.

Objective To investigate the operative techniques for refractory cholelithiasis. Methods The clinical data of 521 patients with refractory cholelithiasis who had been admitted to People's Hospital of Hunan Province from January 1990 to December 2007 were retrospectively analyzed. Results All patients received surgery. After the operation, 3 patients died of liver and kidney failure, the remaining 518 patients were cured without severe complications. The results of B ultrasound and computed tomography showed residual stones in 78 patients (15.1%). Four hundred and twenty-three patients (81.7%) were followed up for 5 months to 17 years (mean, 7.5 years), and the rate of positive effect was 90.1% (381/423). Conclusions Most of the refractory cholelithiasis can be cured radically. Individualized surgical planning, fine and standard surgical procedure are key to the treatment effect.

Objective To investigate publication of papers by nursing staff in a TCM hospital and to find out the problems as well as countermeasures.Method A retrospective analysis about authors,education,academictitle,length of service and age was done to investigate the classes of published papers from 2008 to 2012.Results The academic publication by the nurses from 2008 to 2012 was increased.Among these articles,the TCM nursing techniques took up the largest ratio,which accounted for 29.12%. There were significant differences in ages,length of service and educational background in authors(P<0.05).Conclusions Nurses’ awareness and capabilities of publishing academic papers needs enhancement as well as training.It may be significant to strengthen their incentives in scientific research by enforcing encouraging policies.

Objective To compare the pharmacokinetics of borneolum syntheticum(BS)and borneol in compound Danshen dropping pills in rats.Method Rats were administrated orally with BS or Danshen dropping pills containing equal amount of borneol in a single dose.The blood contents of borneol at a series of time points were assayed by GC-MS and GC-FID after administration.The drug-time curve was estimated by PK Solution 2.0 pharmacokinetic software(USA).Results The pharmacokinetic parameters are quite different between the single dose of BS and the borneol in the compound Danshen dropping pills:the velocity constants of absorption(2.630h-1),distribution(1.788 h-1)and elimination(0.273 h-1)of BS were much greater than those(1.635h-1,0.784h-1,0.116h-1)of the same quantity of borneol in compound Danshen dropping pills,while the Tmax(0.5 h)of former was shorter than that(0.75 h)in the latter,and the Cmax(16.8 ?g/mL)in the former was about half of the latter(31.02 ?g/mL).And,at the 18h after administration,the former blood concentration(0.043 ?g/mL)was approximately one tenth of the latter.Conclusion It is suggested that we should pay attention to the influence of other ingredients on the main objective component in the study of pharmacokinetics of compound traditional chinese drug.

Objective To analyze the distribution of reproductive tract infection(RTI)pathogens and the drug sensitivity of My-coplasma in infertile women.Methods Experimental examinations of the pathogens related to RTI were performed in 260 cases of infertile women(test group)and compared with 260 cases of pregnant women(control group).Results In test group,the positive rate of RTI pathogens was 61.2%.The top 3 pathogens were Mycoplasma (47.7%),Candida (30.0%)and bacterial vaginosis (BV)pathogens(16.7%).There were significant differences of BV pathogens,Mycoplasma,and pH value between test group and control group(P <0.05).And the differences of other pathogens and the cleanliness were not significant between test group and control group(P >0.05).The drug sensitivity rates of Ureaplasma urealyticum to doxycycline and minocin were above 90%,and which to quinolone was less than 40%.Conclusion BV pathogens and Mycoplasma infection is one of the important factors which could affect women infertility.It is necessary to strengthen the monitoring and rational use of antimicrobial agents.

There are no intuitive and unified standards for selecting features in existing protein gel image point matching method, for which an analysis method based on similarity map is proposed. Firstly, the definition and generation methods of similarity map were presented. Secondly, trait and merits of features such as coordinate similarity, shape-context similarity and morphology similarity were analyzed using similarity map method. Finally, comprehensive utilization of multi-features named product-method which has a better effect than mean-method was proposed based on the results. Many experiments using different 2-DE gel images were carried out to prove the validity of similarity map and product-method. The results showed that similarity map could be used for intuitional, effective analysis of matching performance and to guide the selection and comprehensive utilization of multi-features.

Objective To understand the support effect of the resources and services provided by the library on the scientific research of medical postgraduates. Methods Taking 150 graduate stu-dents in Chongqing Medical University as the research object, through questionnaire survey and inter-view method, the characteristics of medical graduate students' selection of document resources, as well as their application of the resources and services provided by the library were investigated. Results 65% (98/150) of students chose Chinese literature as the preferred source of information, 6% (9/150) of graduate students did not borrow books at the library, 54% (81/150) of graduate students chose library website as the data retrieval entrance at the time of obtaining the preferred document, 9% (14/150) of students did not participate in the library reader training. Conclusion Library resources are still the first choice for the graduate students to obtain the literature, and the library should be improved in the aspects of resource allocation, service mode, in order to meet the needs of graduate students' scientific research.