Objective To evaluate the effect of local infiltration anesthesia with different concentrations of ropivacaine on the healing of incision in rats.Methods Seventy-five adult male Sprague-Dawley rats,weighing 220-250 g,were randomly divided into 5 groups (n =15 each):natural healing group (group C),normal saline group (group NS),0.20％ ropivacaine group (group R0.20),0.50％ ropivacaine group (group R0.50) and 0.75％ ropivacaine group (group R075).0.20％,0.05％ and 0.75％ ropivacaine 1.0 ml/kg were injected subcutaneously 10 min before incision in R0.20,R0.50 and R0.75 groups,respectively,while the equal volume of normal saline was given instead in group NS.Five rats in each group were chosen for recording the healing time.Ten rats were taken in each group for recording the length of the incision 72 h after operation.The skin tissues around the incision were removed for determination of fibroblast counts under light microscope,IL-1β content (by ELISA) and the expression of collagen (by immunohistochemisty) and IL-1β mRNA (by RT-PCR).Results Compared with Cgroup,the healing time and length of the incision were significantly shortened,the fibroblast counts and expression of collagen were increased in R0.20 group,the healing time and length of the incision were shortened,the fibroblast counts and expression of collagen were increased,and the expression of IL-1β mRN A and IL-1β content were decreased in R0.50 group,and the healing time and length of the incision were prolonged,the fibroblast counts and expression of collagen were decreased,and the expression of IL-1β mRNA and IL-1β content were decreased in R0.75 group (P ＜ 0.05).Compared with R0.20 group,the healing time and length of the incision were significantly shortened,the fibroblast counts and expression of collagen were increased,and the expression of IL-1β mRNA and IL-1β content were decreased in group R0.50,the healing time and length of the incision were significantly prolonged,the fibroblast counts and expression of collagen were decreased,and the expression of IL-1β mRNA and IL-1β content were decreased in group R0.75 (P ＜ 0.05).Compared with R0.50 group,the healing time and length of the incision were significantly prolonged,the fibroblast counts and expression of collagen were decreased,and the expression of IL-1β mRNA and IL-1β content were decreased in group R0.75 group (P ＜ 0.05).Conclusion Local infiltration anesthesia with 0.20％ and 0.50％ ropivacaine can promote the healing of incision in rats,0.50％ ropivacaine provides better efficacy than 0.20％ ropivacaine,and 0.75 ％ ropivacaine can inhibit the healing process.

Acupuncture Therapy ; Anal Canal ; pathology ; Female ; Humans ; Intervertebral Disc Displacement ; complications ; Middle Aged ; Pain ; etiology ; Rectal Diseases ; etiology ; pathology ; therapy

BACKGROUND: Low power laser irradiation has positive effects on fracture healing, including shortening the time of bone union and enhancement of bone formation. OBJECTIVE: To evaluate the effects of different wave lengths in low power laser on eedy fracture healing. DESIGN, TIME AND SETTING: Randomized, controlled, animal experiment. The study was performed at the Laser Center of Dankook University Medical Center between May 2005 and May 2006. MATERIALS: A total of 60 female English Hartley guinea pigs, 9 weeks old, weighing (300～30) g, were used for this experiment. They were randomly divided into three groups control, 632, and 830 nm groups (n=20). METHODS: The right femoral middle shaft fractures were made with a bone cutter and fixed with intramedullary nails (1.4 mm diameter K-wire). The frequencies of 632 nm and 830 nm semi-conductor laser machines were used. The laser irradiations were started 48 hours after operation vertically at the fracture sites and were applied every two days, at 100 J/cm<'2> of the same irradiation doses and 2.52 cm<'2> of the same irradiation areas in different irradiation groups. Finally, 46 guinea pigs survived, including 14 in control group, 16 in 632 nm group and 16 in 830 nm group. MAIN OUTCOME MEASURES: Guinea pigs were sacdficed at 3 and 6 weeks separately. The bone healing was assessed by the Modified Zorlu Scoring System, including gross, radiologic and histologic examinations. RESULTS: In the gross and radiologic findings, both 632 nm and 830 nm irradiation groups shown a significantly greater rate of callus formation at postoperatively 3 weeks compared to the control group (P< 0.01). Quantity of callus formation in 830 nm group was more than 632 nm group, but there was no significant difference (P > 0.05). The result of histological findings showed a significant increase of osteoblastic proliferation in two irradiation groups than the control group at 3 weeks postoperatively (P < 0.01). Enhancement of osteoblastic proliferation was more obvious in 830 nm group than in 632 nm group at 3 and 6 weeks, but there was no significant difference (P > 0.05).CONCLUSION: Both 632 nm and 830 nm wave lengths are considered to be optimal irradiation wave lengths for eady fracture healing. Moreover, 830 nm irradiation is better than 632 nm irradiation for eady fracture healing.

AIM:To study the effect of mistletoe lectins on cell proliferation and apoptosis in HT-29 human colon cancer cell.METHODS:The effect of mistletoe lectins on cell viability in HT-29 cell was evaluated by MTT assay.Cell apoptosis was observed by DNA ladder analysis and Tunel.RESULTS:Mistletoe lectins(eg.,1-4 mg/L) inhibited cell growth in a dose and time dependent way in HT-29 cells.HT-29 cells apoptosis can be induced when exposed to mistletoe lectins(eg.,1-4 mg/L).CONCLUSION:Mistletoe lectins can inhibits proliferation and induces apoptosis in HT-29 cells in vitro.

Humans ; Medicine, Chinese Traditional ; Syndrome ; Temperature

Objective To study the mechanism of senile escaping of benign prostatic hyperplasia(BPH) condition. Methods With polymerase chain reaction based telomeric repeat amplification protocol assay(TRAP),telomerase activity was examined in 13 normal prostate tissues,35 benign prostatic hyperplasia tissues,33 hyperplastic tubercle and diaphragm. Results Telomerase activity was detected in 2 of 13(15%)normal prostate tissues,17 of 35(49%)benign hyperplasia tissues,14 of 33(42%)hyperplastic tubercle tissues and 1 of 33(3%)diaphragm tissues.The positive rate of telomerase in BPH is higher than normal prostate (P

Objective To analyze the main clinical manifestations of intestinal lipoma and discuss the treatment of intestinal lipoma.Methods The clinical data of 19 patients with intestinal lipoma treated in our hospital in recent 15 years were analyzed retrospectively.CT,ultrasonography,and colonoscopy were used in diagnosis.Local intestinal resection by laparoscopy and laparotomy were employde.Results Intestinal lipoma presented with a variety of symptoms including intestinal bleeding,abdominal pain,anemia,intussusception,and bowel obstruction.In 17 of the intestinal lipoma patients,the main clinical manifestations were change of bowel habits,abdominal pain and bloody stool;13 of them had intestinal intussusception or obstruction,and two patients were asymptomatic.Sixteen patients underwent colonoscopy,and seven of them were correctly diagnosed.Seventeen patients received surgical resection and recovered.The diameter of mass was 3-6.5 cm.The pathologic exam showed submucosal lipoma in 12 cases,two cases showed ulcer formation with erosion,2 cases had intermural lipoma and one case had atypical lipoma with potential malignant transformation.Two asymptomatic patients diagnosed by colonoscopic biopsy werre only followed up at regular intervals.Conclusions Imaging diagnosis and colonoscopy are helpful in the preoperative diagnosis of intestinal lipoma.The method of treatment is based on the size of the tumor and presence or absence of pedicle.Endoscopic electrosection is often used for tumor mass less than 3 cm in diameter,otherwise local intestinal resection is the treatment of chocie.

OBJECTIVE:To prepare compound Weifu emulsion and to observe its therapeutic effect on infantile eczema METHODS:The emulsion was prepared with a formulation consisting of Scutellaria,Chinese Angelica,Chuanxiong and dimethyl silicone oil etc and the quality control method was established RESULTS & CONCLUSION:This preparation is stable in quality and feasible in quality control;The effective rate in treating infantile eczema has reached 90% and no obvious adverse reactions were found This emulsion is worthy of recommendation for clinical use

The paper analyzes the application advantages of information technology in medical informatics education.Takes Shanghai Sipo Polytechnic College as an example, it elaborates the specific application of information technology in medical informatics education, including immersion education based on 3D virtual reality technology, two-way video teaching and other aspects.It also gives solutions on current existing problems.

Objective To analyze the causes of surgical errors and complications of lumbar degenerative diseases treated by transforaminal endoscopic spine system(TESSYS),and explore the treatment and prevention measures.Methods From July 2012 to January 2016,the data of 660 patients with lumbar degenerative diseases who treated with TESSYS were analyzed retrospectively,of which 528 patients were lumbar disc herniation with 192 cases of single segment(L4/L5) and 336 cases of multi-segment.Lumbar spinal stenosis in 132 cases,were lateral recess and nerve root canal stenosis.Surgical errors and complications were recorded,and analyzed the reasons and the treatment and preventive measures.Results Intraoperative errors:1 case of sterile film with the dilating catheter went into the surgical area;1 case with wire fracture,and stump retention in vivo;for discography staining,the proportion of mixed solution was not enough,the leakage of excess concentration of methylene blue caused caudal nerve injury in 3 cases;expansion of the intervertebral foramen,the drilling part of the articular process was too deep and too close to the medial wall of the articular process,which caused cutting injury of nerve fiber sheath in 2 cases;after the rupture of the dura mater,the rinse solution was perfused,caused bloating,elevated intracranial pressure,elevated blood pressure and other symptoms of spinal cord hypertension in 3 cases;low dose of local anesthetic into the blood caused toxic reaction in 2 cases;total spinal cord anesthesia with paravertebral nerve root block in 1 case.Postoperative complications:3 cases of nerve root edema;2 cases of free nucleus pulposus tissue;2 cases of local hematoma led to nerve root compression;1 case of intervertebral space infection and 3 cases of local skin neuritis in the puncture area.Conclusion In the process of intervertebral foramen surgery,there are some deficiency on instruments or operating skill.Therefore,rigorous treatment of each link in the perioperative period,is the guarantee of successful surgery.