Bidding invitation and procurement of medical equipment is one of the most important works in hospital.It can increase the working efficiency to hold the key taches of medical equipment procurement from 4 aspects including the science of decison-making,the comfirmability of bidding invitation,the right choice of the way of bidding,etc.So some important taches of medical equipment procurement are analyzed and discussed and some points for attention are raised.[Chinese Medical Equipment Journal,2008,29(2):76-77]

ncreased. But it remains controversial how vaspin is correlated with insulin sensitivity. More studies based on large population are needed to identify the associ-ation between vaspin and insulin sensitivity.

Astrocyte elevated gene (AEG) -1 is cloned as a human immunodeficiency virus (HIV) -1-inducible and tumor necrosis factor-alpha (TNF-α)-inducible transcript in primary human fetal astrocytes (PHFA) by a rapid subtraction hybridization approach. AEG-1 has been reported to be up-regulated in various types of human cancers. Multivariate analyses indicat that AEG-1 correlates with the ability of growth, invasion, metastasis, angiogenesis and chemoresistance of tumors. AEG-1 over-expression activates the PI3K-Akt,nuclear factor kappa B (NF-κB) and Wnt-β-catenin signaling pathways in several crucial aspects of tumorprogression. AEG-1 represents a viable potential target for the therapy of human cancers.

Objective: To investigate the expression and subcellular localization of G protein-coupled receptor 30 (GPR30) in Ishikawa endometrial adenocarcinoma cells. Methods: The expressions of GPR30 mRNA and protein in Ishikawa cells were detected by RT-PCR, Western-blotting and immunofluorescence assay. The positive expression rate of GPR30 in the cell membrane and cytoplasm was determined by flow cytometry (FCM). The subcellular localization of GPR30 labeled with colloidal gold was observed under a transmission electron microscope. Results: The expressions of GPR30 mRNA and protein were both detectable and predominately localized in the cell membrane and cytoplasm of Ishikawa cells. The positive expression rate of GPR30 protein in the cytoplasm [(20.10±0.13)%] was significantly higher than that in the cell membrane [(8.54±0.17)%] (P < 0.01). The subcellular localization of GPR30 protein was mainly in the mesh-like network of cytoplasm, which may refer to rough endoplasmic reticulum. Conclusion: The expression level of GPR30 is higher in cytoplasm than in cell membrane of Ishikawa cells. GPR30 is predominantely localized in the rough endoplasmic reticulum of Ishikawa cells, which suggests an important role of GPR30 in endometrial adenocarcinoma. Copyright© 2011 by TUMOR.

Dental Implantation ; Humans ; Surgery, Computer-Assisted

OBJECTIVE To investigate the clinical distribution and antibiotics resistance of Pseudomonas aeruginosa nosocomial infection after open-heart operation.METHODS The clinical data of 393 patients with nosocomial infection from Jan 2004 to Dec 2007 were analyzed retrospectively.RESULTS There were 57 infected cases caused by P.aeruginosa,and accounted for 14.5% of all infections during period.All infected patients had serious original heart diseases,and received broad-spectrum antibiotic therapy previously.Forty-two patients developed postoperative cardiorespiratory function failure,and 28 patients needed circulatory support.Thirty-six patients prolonged mechanical ventilation time for over 1 week.Results of susceptibility test showed that ciprofloxacin,levofloxacin and piperacillin/tazobactam were the most active antibiotics,followed by tobramycin,netilmicin,gentamicin,meropenem and imipenem/cilastatin.P.aeruginosa presented high resistance to ceftazidime and cefoperazone/sulbactam.CONCLUSIONS P.aeruginosa is one of the most common pathogenic bacteria after open-heart operations in our hospital and presented multidrug resistance.Rational use of antibiotics is important to reduce drug resistant strains.

Heart ; drug effects ; Heart Failure ; drug therapy ; Humans ; Hydrogen Bonding ; Nitrogen Oxides ; pharmacology ; therapeutic use

Chromosome Aberrations ; Environmental Exposure ; adverse effects ; Humans ; Magnetic Fields

4 mm in diameter, re-operations of ESWL were required until no stones were detected. Results All the patients presented a good tolerability to the treatment and left for home by themselves. With exception of 1 case of failed lithotripsy, the ESWL was successfully accomplished in the rest of 17 cases after receiving 3~9 times of treatment. One or two times of hematuria occurred in 66 times of treatment (66/77, 85.7%). A “stone terrace”, 1.9~5.2 cm in length, was found in 5 cases, 4 of which were cured by re-operation of ESWL and 1 of which underwent ureteroscopic removal of stones. Conclusions Low-energy extracorporeal shock wave lithotripsy is a safe and effective option for patients with renal staghorn calculi.

Objective To explore the effectiveness of modified Sundaresans method, including exposure through the incision involving both of the sternoclavicular joint and lower cervical region with sternum retention, in the treatment for upper thoracic spinal tumor. Methods From January 1999 to October 2003, 14 patients, 9 males and 5 females, aged from 26 to 65 years with a mean of 43 years underwent the modified Sundaresans procedure. The anterior cervico-thoracic incision was made, the medial part of clavicle close to the sternoclavicular joint was removed. After the exposure of the tumor, the involved vertebral body and contiguous discs were resected, the spinal cord was decompressed, then followed by implanting part of resected clavicle or titanium mesh with an anterior locking plate system. Neurological status was graded according to Frankel grading system. Results The duration of follow-up ranged from 6 months to 2 years with an average of 10 months. This approach displayed the upper thoracic region clearly for removal of the tumor and internal fixation,in avoiding the neurological injury. All patients had their neurological improvement at different level. According to Frankel grading system, one patient improved from grade A pre-operation to B post-operation, while another patient from B to D. Of 2 cases with grade C before operation, one improved to D and the other to E. Of 4 patients with grade D, 2 improved to E, while the other 2 left unchanged. There were 6 cases keeping the same grade before and after operation. Resection of sternoclavicular joint almost had no effect on the movement of shoulder. The abduction of the shoulder joint in 14 patients from 90?-130?(average, 125?) preoperatively became 80?-120?(average, 110?) postoperatively. Conclusion The modified Sundaresans method for the upper thoracic tumors has many advantages, such as satisfactory outcome, less invasion and no loss of the shoulder function. It is especially indicated to the tumors, where the spinal compression is located anteriorly.