BACKGROUND: Because dopaminergic neurons are particularly sensitive to oxidative stress, microglia is characteristics of being prone to activation, and activated microglia is the main source of oxygen free radical production, so microglia activation is more important in the pathogenesis of Parkinson's disease and illness progress.OBJECTIVE: To summarize the correlation between microglia and Parkinson's disease.METHODS: An online computer-based retrieval was performed by the first author among the Chinese Journal Full-Text Database (CNKI: 2000/2010) and Medline (2000/2010) Database, with key words of Parkinson's disease, microglia in English and Chinese. The correlation between microglia and Parkinson's disease was discussed through two aspects, one is the influence of cell factors and toxic substance produced after microglia activation on Parkinson's disease, the other is the inhibition of microglia and prevention of nerve toxic factors on Parkinson's disease progress.RESULTS AND CONCLUSION: A total of 112 articles were screened out according to inclusion and exclusion criteria, and 27 of them were involved in the analysis. Results showed that microglia activation will damage dopaminergic neurons, and cause Parkinson's disease. And the occurrence and development of Parkinson's disease may further reduce the neurotransmitter dopamine, continue to damage dopaminergic neurons and release the inflammatory factor, thus promoting microglia activation. Inhibition of microglia activation is likely to stop the progress of Parkinson's disease.

Objective:To observe the efficacy and safety of high dose methylprednisolone in the pulse therapy for infantile spasms. Methods:Totally 70 cases of infantile spasms were randomly divided into the conventional dose group and high dose group with 35 ones in each according to the random number table. High dose group was given methylprednisolone pulse therapy for the first 5 days, and then received the conventional dose treatment. The conventional dose group received prednisone tablets orally. The treatment course was 8 weeks. The ECG outcome, efficacy and incidence of adverse reactions were observed and compared between the groups, and the efficacy of pulse therapy for the patients with different causes and different course was also studied. Results:The total effective rate of high dose group was 94. 29%, which was significantly higher than that of conventional dose group (71. 43%), and the difference was statistically significant (P<0. 05). The EEG outcome between the groups had not statistical significance (P>0. 05). The incidence of adverse reactions in high dose group was wigher, while the difference was not significant (P>0. 05). The efficacy of pulse therapy for different causes and different course had no statistically significant difference (P >0.05). Conclusion: High dose methylpred-nisolone pulse therapy can effectively improve clinical efficacy for infantile spasms, which shows no effect on the safety of patients.

OBJECTIVE To study the washing effect of three different methods with the multienzymatic detergent to keep the surgical instruments moist.METHODS The surgical instruments which were selected within five days were washed by three different methods with the multienzymatic detergent to keep moist.The washing effect was compared by eyes and with 5 times magnifying glass and with OB test paper.RESULTS There were prominent difference to washing effect among three methods to keep the surgical instruments moist(P

This article introduces a measurement circuit for Respiratory Mechanics Parameters which is based on differential pressure sensor. The circuit detects the flow and airway pressure signals from the sensors, then more than ten Respiratory Mechanics Parameters are identified. The system meets the clinical need of Respiratory Mechanics measurement, and improves the performance of domestic Patient Monitor.

OBJECTIVE:To explore the therapeutic effect of Chinese herbal compounds Zhiganle on fatty liver induced by high-cholesterol high fat diet in rats and the action mechanism.METHODS:Wistar rats were randomized into 6 groups:normal group,model group,Dongbao gantai group,ZGL groups(high,middle,and low doses).Except the normal group,the other 5 groups of rats were administered with high-cholesterol high fat diet for 12 weeks.On week 13,the ZGL groups were administered intragastrically with Zhiganle(1.5,3.0,4.5 g?kg-1) and rats in Dongbao gantai group with Dongbao gantai(0.3 g?kg-1) qd for 12 consecutive weeks.The hematological indexes were measured and the pathological change in rats was observed.RESULTS:In ZGL groups,serum levels of total cholesterol(T-CH) and triglyceride(TG) and the MDA level in liver were decreased significantly(P

OBJECTIVE: To improve the management level in outpatient dispensary.METHODS: The current management model in each outpatient dispensary of our hospital was introduced;the problems were pointed out and the proposals were put forward.RESULTS & CONCLUSIONS: The current outpatient pharmacy management mode and drug control measures of "individual responsibility,weekly small stocktaking,monthly big stocktaking,and irregular spot checks" is feasible,if optimized further,which would help enhance the working quality and service level in pharmacy.

Objective:To investigate the effect of flexible visiting policies in ICUs by comparing with restrictive visiting policies.Methods:English database (Pubmed, embase, Cochrane library, scopus, OvidSP, Wiley Online library, Google scholar and web of science) and Chinese database (CNKI, VIP, CBM and Wanfang) are retrieved, the search time is up to February 2020. The included literatures were evaluated quantitatively and qualitatively.Results:The flexible visiting policies can reduce the risk of delirium in ICU patients [RR=0.59, 95%CI=(0.36,0.96)], shorten the length of stay [MD=-0.21, 95%CI=(-0.35,-0.06)], improve patients' anxiety symptoms [MD=-2.2, 95%CI=(-3.8,-0.61)], reduce the incidence of anxiety and depression and improve the nursing satisfaction of patients and their families, without increasing the risk of ICU acquired infection [RR= 0.77, 95% CI= (0.51, 1.16)] and patient mortality (RR= 0.82, 95% CI= (0.53, 1.26)]. Sensitivity analysis suggested that the results were stable.Conclusions:Compared with the current restrictive visitation policies, the flexible visitation system can reduce the risk of delirium, improve anxiety symptoms and depression, and obtain more satisfaction from family members and patients, and It can be tried further.

Objective:To investigate the values of immunophenotype and the Collagen type1 alpha1/Proto-oncogene Proteins c-sis (COL1A1/PDGFB) fusion gene in the diagnosis of dermatofibrosarcoma protuberans (DFSP). Methods:IHC markers and the COL1A1/PDGFB fusion gene were detected by IHC staining and interphase fluorescence in situ hybridization (FISH) in 73 cases previously diagnosed as DFSP. A total of 85 and 10 non-DFSP cases were also included as controls for IHC staining and FISH, respectively. Results:In the 73 DFSP cases, the positive detection rates for immunohistochemical marker vimentin, CD34, CD99, S100, desmin and SMA were 100%, 91.78%, 61.64%, 0, 0, and 6.85%, correspondingly. Protein expression levels in these cases varied from the control group, and CD34 ex-pression was significantly different among the differential diagnoses. The positive detection rate for the COL1A1/PDGFB fusion gene was 86.96%(60/69), whereas the gene expression in the control group was negative. Conclusion:The COL1A1/PDGFB fusion gene is a highly specific and sensitive marker in the diagnosis of DFSP. CD34 is a suitable marker for DFSP.

Objective To observe the clinical efficacy and safety of immediate-effect moxibustion combined with massage for the treatment of knee stiffness after knee ligament reconstruction surgery.Methods A total of 138 patients after knee ligament reconstruction surgery were randomly divided into control group and observation group,69 cases in each group.The control group received joint rehabilitation therapy,which included TDP light radiation,joint exercise by continuous passive motion apparatus,contraction and relaxation of quadriceps femoris muscle and ankle pump.The treatment group was given immediate-effect moxibustion combined with massage based on the treatment for the control group.The treatment lasted from postoperative day 3 to 14.Before and after treatment,Lysholm knee joint scores,joint activity scores and activities of daily living(ADL) scores of the two groups were observed.Results After treatment,the Lysholm knee joint scores,joint activity scores and ADL scores of the two group were obviously increased (P ＜ 0.05 or P ＜ 0.01 compared with those before treatment),and the increase in the observation groups was superior to that in the control group,the differences of the alove indexes except for joint activity scores being statistically significant between the two groups (P ＜ 0.05 or P ＜ 0.01).Conclusion Immediate-effect moxibustion combined with massage is effective on preventing knee from postoperative ankylosis after knee ligament reconstruction surgery.

Objective To study the method of preparation of donor liver in liver transplantation. Methods The methods and skills of donor liver preparation and the anomaly artery reconstruction of graft in 64 cases of orthotopic liver transplantation (OLT) were retrospectively analyzed. Results All allografts had preparation and were suitable for clinical transplantation. Thirteen cases with hepatic artery anatomy variation were found. Among the 13 cases, 5 cases were reconstructed. Splenic artery (3/5) and gastro-duodenal artery (2/5) were typically used for anastomosis of the variant hepatic arteries. No complications resulted from donor liver preparation. Conclusions Correct preparation of the donor hepatic artery and biliary tracts, can decrease the incidence of hepatic artery and biliary tract complications after liver transplantation, and is the key to ensure successful donor liver preparation.