Orphan nuclear receptor steroidogenic factor 1 (SF-1) is a member of the nuclear receptor superfamily. It regulates the expression of multiple genes involved in adrenal and gonadal development,steroidogenesis,and reproductive function. Twenty five different mutations have been described and their clinical features are various. Detection of SF-1 gene may be contribute to the etiological diagnosis for 46, XY disorder of sex development patients.

In recent years,it has been reported that the osteopontin attracts great attention for its roles in tumor growth and metastasis.In many tumors,osteopontin plays an important role in promoting tumor growth,metastasis and enhancing adhesion and migration abilities.In particular,in hepatocellular carcinoma,osteopontin has very satisfactory sensitivity and specificity in early diagnosis and is relatively effective on prognosis prediction.Suppression osteopontin expression will inhibit liver cancer growth and metastasis,indicating that the osteopontin stays a new target for liver cancer treatment and possesses intangible clinical potential.

Objective To investigate the clinical manifestations and imaging features of moyamoya disease (MMD).Method The clinical manifestations and the results of CT angiography ( CTA ) and digital subtraction angiography (DSA) examinations of 162 cases diagnosed with M MD were analyzed retrospectively.Results In 162 patients with MMD, 132 cases (81.48% ) had stroke, in which cerebral infarction and transient ischemic attack (TIA) were 20 cases, intraventricular hemorrhage were 48 cases, cerebral hemorrhage were 52 cases, subarachnoid hemorrhage (SAH) were 10 cases and acute subdural hemorrhage were 2 cases; 8 cases(4.94%) had simple migraine and 22 cases(13.58%) had simple epilepsy.All the patients were with typical imaging findings: ( 1 ) The central branch of the internal carotid artery,bilateral internal carotid artery clinoid segment and proximal arterial stenosis or occlusion was the main performance of MMD.(2) Abnormal vascular network in the skull base was the most feature of MMD changes.(3) The performance of collateral circulation.Conclusions CTA and DSA can be used for the diagnosis of MMD patients.DSA is the gold standard for diagnosis of MMD.

Vildagliptin is a potent and specific inhibitor of dipeptidyl peptidase 4,which has been implicated to enhance and prolong the physiological actions of incretion hormones including glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptide.Vildagliptin is an orally administered drug and has been licensed for the treatment of type 2 diabetes mellitus.Vildagliptin monotherapy or in combination with other drugs can effectively lower glycosylated haemoglobin levels to a great extent.In addition,vildagliptin acts in a glucose-dependent manner,explaining its low risk of hypoglycemia.This drug has been proved well tolerated with few gastrointestinal side effects or oedema.Therefore vildagliptin is a promising new option for type 2 diabetic patients,and these patients would achieve better glycemic control and fewer complications in the long run.

OBJECTIVE: To investigate the effects of Astragalus injection (AI) on basal-like breast cancer cell line MDA-MB-468 and murine bone marrow stromal stem cells (mMSCs). METHODS: MDA-MB-468 cells and primary cultured mMSCs were treated by different concentrations of AI, and with untreated MDA-MB-468 cells as blank control. The morphology of cells was observed by phase-contrast inverted microscope and transmission electron microscopy. Cytotoxic effects of AI on MDA-MB-468 cells and mMSCs were evaluated by cell counting kit-8 (CCK-8) assay. Cell cycle and apoptosis of MDA-MB-468 cells induced by AI were measured by flow cytometry. Lactate dehydrogenase (LDH) activity in supernatants was measured by enzymatic colorimetric method. The expressions of epidermal growth factor receptor (EGFR) and p53 protein in MDA-MB-468 cells were evaluated by streptavidin-biotin-peroxidase complex method. RESULTS: A time-dependent cytotoxic effect of 1 g/ml AI was observed in MDA-MB-468 cells. 1 g/ml AI also had cytotoxic effect on mMSCs, but its effect was not better than cisplatin. 0.1 g/ml AI could promote the proliferation of mMSCs. Different concentrations of AI could all induce the apoptosis of MDA-MB-468 cells. There was no significant difference in LDH activity in the supernatants between blank control group and AI-treated and cisplatin-treated groups. AI could down-regulate the expressions of EGFR and p53 protein. CONCLUSION: The effects of AI on MDA-MB-468 cells and mMSCs are related to the concentration of AI, and its mechanism of inhibiting the proliferation of MDA-MB-468 cells may be due to down-regulation of the expressions of EGFR and p53 protein.

Astrocyte elevated gene (AEG) -1 is cloned as a human immunodeficiency virus (HIV) -1-inducible and tumor necrosis factor-alpha (TNF-α)-inducible transcript in primary human fetal astrocytes (PHFA) by a rapid subtraction hybridization approach. AEG-1 has been reported to be up-regulated in various types of human cancers. Multivariate analyses indicat that AEG-1 correlates with the ability of growth, invasion, metastasis, angiogenesis and chemoresistance of tumors. AEG-1 over-expression activates the PI3K-Akt,nuclear factor kappa B (NF-κB) and Wnt-β-catenin signaling pathways in several crucial aspects of tumorprogression. AEG-1 represents a viable potential target for the therapy of human cancers.

fulminant liver failure(FLF)is a rapid onset life-threatening emerency in which liver dysfunction,liver failure and hepatic encephalopathy occur within 8 weeks in previouslv norillal person.It has been confirmed by available clinical experience and literatures during the past decades that FLF patients are ideal recipients of liver transplantation.However,the prognosis of the FLF patients who accepted liver transplantation differed greatly according to the available reports because of the differences of supportive treatment during waiting period,the criterion for recipient assessment,the determination of operation chance,the use of critical techniques during operation and postoperative management between different transplantation centers.We probe the standardized clinical use of the aspects above mentioned in liver transplantation for FLF,particularly discuss the treatment of complications resulting from coagulopathy,determination of operation chance,significance of standardized etiological treatment and the indication of the use of veno-venous bypass technique during transplantation to provide better treatment strategy and improve the prognosis of FLF patients.

Stress urinary incontinence is a common and frequently disease among middle and old women.However,it did not cause enough attention by patients.In recent years,people have learned more about incontinence mechanism,and designed some tension-free vaginal tape operation.Those operations have showed excellently effects.We introduce some highlights about stress urinary incontinence to help reader understand the disease,such as operation principle,influencing factors about surgery,indications,concomitant disease and surgical treatment for failure cases,etc.

OBJECTIVE To analyze the prevalence of pathogens in liver abscess and their resistance.METHODS All specimens of liquor puris were collected from patients with liver abscess by liver puncture in Union hospital,Fujian Medical University from Jan 2002 to Dec 2007.Pathogens were isolated,and followed by antibiotics resistance assay.RESULTS The key pathogens of liver abscess were Gram-negative bacteria.The main Gram-negative bacteria were Escherichia coli and Klebsiella pneumoniae.The main Gram-positive bacteria were Streptococcus,Enterococcus and Staphylococcus.The resistance of K.pneumoniae was increased in recent years.CONCLUSIONS The bacterial species in the liver abscess are varying in recent six years,the ESBLs are the important factor of the resistance to antibiotics.

Randomly divide the said patients into 2 groups,32 cases in treatment group treated with head skin and abdominal acupuncture,body acupuncture for the control group of 28 cases.The result shows the cure rate is 56.25% for treatment group,37.5% for control group.By comparison,P