Neuroblastoma (NB)is a common malignant solid tumor in childhood,which has various clinical manifestations.It is difficult to diagnose and to prone to early metastasis.It is one of the main diseases causing death in childhood.The treatment of children with high-risk NB is still very challenging.At present,the treatments of NB mainly concentrated in the surgery,chemotherapy,radiation therapy and transplantation technology,etc.In recent years,more studies focus on targeted therapy and molecular biology (genetics) treatment.Targeted therapy is targeted to different specific loci of tumor,so as to kill tumor cells and reduce the impact on the normal tissue,which supplies more safe and effective treatments for children with NB.

Objectives: To investigate the effects of the extract mixture of alfalfa and perilla on lowering serum lipid, protecting liver and aortic wall in rats. Methods: Sixty SD rats were divided into six groups and were fed different diet. After seven or fourteen days, the rats were put to death and measured for serum lipids, cholesterol content of liver and aortic wall, and pathological change of the tissue. Results: There was a significant increase of serum TC, LDL-C, cholesterol content of liver and aortic wall in the high-fat fed rats. Under light microscope, there were lipid droplets and cholesterol crystal in liver of group high-fat. Compared with the group high-fat, the rats had a significant decrease of those measurements in serum or in tissue in the alfalfa and perilla group. Conclusions: The extract mixture of alfalfa and perilla could prevent the increase of serum TC, LDL-C, cholesterol content of liver and aortic wall in high-fat fed rats, and also prevent the fatty infiltration and cholesterol deposit of liver.

Five inner ears were removed from five rabbits under aneasthesia. The hard, thick bones of outer and middle ears were trimmed off, and the auditory ossicles were taken away. The cochleae were fixed with 2％ glutaraldehyde in phosphate buffer for eight hours, then they were decalcified in 10％ EDTA-2Na solution for ten days, and the softened shells of the cochleae were stripped off, and post-fixed in 1％ OsO_4 for an hour, dried at critical point, coated with gold, and observed under SEM.The rabbit cochlea shows two and a half turns, the modiolus is about 3.5mm high, and the organ of Corti lies on the basilar membrane of the spiral lamina. The outer hair cells usually form three parallel rows on the middle and basal turns but occasionally four rows on the top half turn. The inner hair cells form only a single row. Every bunch of the outer hairs consists of about 93-114 stereocilia which together show a "W" shape. Every bunch of inner hairs has about 50 cilia which together show an arch shape. The cilia on the top turn are longer than those on the base. The length and width of the inner and outer hair cells and four other kinds of cells were measured according to photos, and the numbers of these kinds of cells were estimated.Other three cochleae fixed at the time of 1, 2 or 4 hours after death were also observed. In the cochlea fixed four hours after death, the majority of the auditory hairs were found fallen down, which means the fixation of cochlea should be carried out within less than four hours after death.Some of the structural similarities and differences of cochleae among rabbit,human being and guinea pig were also compared in this paper.

Objective To observe and analyze curative efficacy of Desloratadine Citrate Disodium in combination with endoscopic sinus surgery and its effects on serum TIgE, ECP and inlfammatory factors in patients with chronic sinusitis.Methods 90 patients with chronic sinusitis who received therapy in our hospital from January 2015 to June 2016 were collected as research objects to be randomly divided into the control group and the observation group equally. The control group was given endoscopic sinus surgery, and the observation group was given Desloratadine Citrate Disodium in combination with endoscopic sinus surgery. Then, visual analogue scale (VAS) was applied to assess the severity of subjective symptoms of the disease. Three months after the surgery, the curative efifcacy, scores of visual analogue scale (VAS) relfecting clinical symptoms, levels of serum TIgE, ECP and inlfammatory factors were compared between the two groups.Results All patients undergone successful surgery. The observation group had a total therapeutic efifciency ratio of 95.6%, which was signiifcantly higher than that of 82.2% in the control group (P < 0.05). In comparison with the control group, the observation group had lower VAS scores of nasal discharge, nasal obstruction, cheek pain, headache, hyposmia [(1.84 ± 0.89) vs (3.12 ± 1.03) points, (1.87 ± 0.81) vs (2.55 ± 1.14) points, (1.24 ± 0.86) vs (1.88 ± 0.79) points, (1.15 ± 0.54) vs (1.97 ± 0.83) points, (1.84 ± 0.73) vs (2.18 ± 0.84) points], lower levels of serum TIgE, ECP [(58.62 ± 7.36) vs (64.39 ± 7.18) kU/L, (4.25 ± 0.84) vs (5.01 ± 1.07) ng/L)] and inflammatory factors of TNF-α, IL-6, IL-8 [(1.27 ± 0.46) vs (2.62 ± 0.53) ng/ml, (5.13 ± 1.24) vs (9.54 ± 2.23) pg/ml, (282.63 ± 32.42) vs (315.84 ± 35.89) ng/L)] with statistical differences (allP < 0.05).Conclusion Desloratadine Citrate Disodium in combination with endoscopic sinus surgery is effective for chronic sinusitis, which can signiifcantly relieve clinical symptoms, reduce levels of serum TIgE, ECP and alleviate inlfammatory reaction.

Objective To correlate the neutrophil extracellular trap (NET) mitochondrial DNA (mtDNA) and anti-mtDNA antibodies (Abs) with disease activity and clinical features in systemic lupus erythematosus (SLE) patients.Methods We enrolled 102 SLE patients,rheumatoid arthritis (RA) patients (n=30) and healthy donors as controls (n=40).NET were generated from phorbol 12-myristate 13-acetate (PMA)-stimulated peripheral neutrophils.mtDNA levels and the transcriptional levels of five interferon inducible genes(IFIGs)(OAS-1,Mx-1,Ly6e,IFIT1 and IFIT4) were measured by quantitative PCR.Interferon scores (IFN scores) were calculated.Anti-mtDNA Abs were detected by enzyme-linked immunosorbent assay.Spearman's correlation analysis,t test andx2 test were used for statistical analysis.Results mtDNA release by netting neutrophils was greatly enhanced in SLE patients (1 088 000 ±1 133 000) compared with healthy controls (465 900±447 200)(t=2.617,P＜0.01) and significantly correlated with IFN scores (r=0.460 6,P＜0.01).NETs mtDNA in moderate active group (728 300±1 003 000) and severe active group (1 093 000±946 500) were significantly higher than the mild active group (159 500±155 100) (t=2.240,P＜0.05,t=3.894,P＜0.01).Forty-one percent of SLE patients were positive for anti-mtDNA Abs(1.28±0.68),while none of the healthy donors (0.70±0.31) (P＜0.01) and RA controls (0.59±0.18)(P＜0.01) displayed a positive serology response to mtDNA.Addition-ally,the titers of anti-mtDNA Abs were also associated with IFN scores (r=0.292 8,P＜0.05).Anti-mtDNA Abs in moderate active group (1.3±-0.6) and severe active group(1.4±0.7)were significantly higher than the mild active group (0.7±0.4) (t=3.154,3.538,all P＜0.01).The levels of anti-mtDNA Abs significantly correlated with classic an-ti-dsDNA Abs titers measured by Farr assay (r=0.542 9,P＜0.001) and were associated with LN (x2=8.644,P＜0.01).Conclusion mtDNA in NET and anti-mtDNA Abs serve as new biomarkers for disease activity and renal involvement in SLE patients.

Through the network medical teaching and guidance system,the live video of clinical medicine activity can be multicast by MPEG2 code and MPEG4 code simultaneously on the LAN or WAN.It may meet the request of different network bandwidth,realize the bidirectional audio or video frequency at the same time,and satisfy the clinical teaching or guidance need.The client port can be deployed in the random terminal on LAN or WAN,while the user with the authorization can see the high clear surgery image in real time at any place,make communication through the bidirectional audio and video system,and even remotely control the camera's lens angle and focus for perfect video effect.

Objective:To study the bidimensional model of self-esteem and the relationship between self-esteem and self-consistency and congruency.Method:591 college students completed Rosenberg Self-Esteem Scale and Self-Consistency and Congruency Scale. Amos4.0 was applied to analysis the unidimensional model and the bidimensional model of self-esteem, regression between self-esteem and self-consistency and congruency were then analyzed.Results:The bidimesional model of self-esteem fit the data better (?2=126.52,df=34, ?2/df=3.72, CFI=1.00, TLI=0.99, RMSEA=0.07), and dissociation effect between self-consistency and congruency and positive dimension and negative dimension were found.Conclusion:The study tends to support the hypothesis of the bidimensional model of self-esteem.

PurposeTo evaluate the shear wave elastography in diagnosis of intraductal papillomas, benign or malignant breast lesions.Materials and Methods From December 2013 to December 2014, 145 female patients with 155 lesions who underwent ultrasonography and shear wave elastography in general hospital of PLA were retrospectively studied. All the lesions were confirmed by pathology via surgery or biopsy. The performance of the maximum elasticity, the mean elasticity, the minimum elasticity values and the elasticity ratio in diagnosis of benign and malignant tumors were analyzed. The portion of intraductal papillomas in misdiagnosed cases was calculated. The elasticity values of intraductal papillomas were compared with those of other breast benign and malignant lesions.ResultsAmong 155 lesions, pathology confirmed intraductal papillomas in 20 lesions, fibroadenosis in 23 lesions, fibroadenoma in 23 lesions, inflammation in 9 lesions, adenosis with fibroadenoma in 17 lesions, and breast cancer in 63 lesions. The maximum, mean, minimum elasticity values and the elasticity ratio of intraductal papillomas were (84.39±74.08) kPa, (51.33±46.36) kPa, (26.55±24.98) kPa and 2.80±2.18, respectively. The elasticity values of intraductal papillomas were between those of breast cancer and other benign lesions, and there were no statistically significant (P=0.003).ConclusionThe elasticity values of intraductal papillomas were between those of breast cancer and other benign lesions, which may be a potential reason to cause misdiagnosis using shear wave elastography to differentiate breast benign and malignant diseases.

OBJECTIVETo observe treatment efficacy of acupuncture at different distal acupoints for acute lumbar sprain after massage.

METHODSOne hundred and fifty patients with acute lumbar sprain were randomly divided into a Yaotongdian (Extra) group, a Houxi (SI 3) group, a Weizhong (BL 40) group, a Chengshan (BL 57) group and a Shuigou (GV 26) group, total 5 groups, 30 cases in each one. After the same massage treatment and based on groups divided, different distal acupoints above-mentioned were selected to be acupunctured. Visual analogue scale (VAS) scores, lumbar activity and treatment efficacy of patients in 5 groups were evaluated.

RESULTSWith acupuncture at distal acupoints after massage,VAS scores of patients in 5 groups were decreased compared with those after massage treatment (all P<0.05); lumbar activity was all obviously improved (all P<0.01); and cured and markedly effective rates were all increased in comparison with those after massage (all P<0.01). But among 5 groups the differences of VAS scores, lumbar activity and cured and markedly effective rates were not statistically significant(all P>0.05).

CONCLUSIONBased on massage treatment, acupuncture at distal acupoints could further improve the treatment efficacy for acute lumbar sprain, but there is no obvious effective difference among every distal acupoint. As long as choosing acupoints with lower pain threshold and stimulating enough, good efficacy could be acquired for acute lumbar sprain.

Acupuncture Points ; Acupuncture Therapy ; Acute Disease ; therapy ; Adolescent ; Adult ; Aged ; Combined Modality Therapy ; Female ; Humans ; Lumbosacral Region ; injuries ; Male ; Massage ; Middle Aged ; Sprains and Strains ; therapy ; Treatment Outcome ; Young Adult

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Lymphatic Diseases ; etiology ; pathology ; Male