Objective To explore the efficacy and adverse reaction of nedaplatin (NDP)+gemcitabine (GEM)and cisplatin (DDP)+GEM for advanced lung squamous cell carcinoma.Methods A total of 1 01 cases advanced untreated patients from September 201 2 to December 201 3 were randomly divided into 2 groups using random number table method:69 patients in the observation group accepted NDP+GEM treatment and 32 patients in the control group received DDP +GEMtreatment.The objective response rate (RR),disease control rate (DCR ) and progression-free survival (PFS ) and adverse reaction were collected and evaluated. Results RR was 28.6%(1 8/63)in the observation group and 1 5.6%(5/32)in the control group,DCR was 81 .0%(51/63)in the observation group and 68.8%(22/32)in the control group (χ2 =1 .36,P=0.24;χ2 =1 .67,P=0.20).The median PFS was 4.52 months and 4.01 months in the observation group and control group (χ2 =0.09,P=0.73).The major adverse reaction was myelosuppression in both groups (33.3% vs.37.5%,χ2 =0.1 7,P=0.68).The incidence ofⅢ-Ⅳ grade nausea and vomiting was lower in the observation group, compared with the control group (1 4.5%vs.56.3%,χ2 =1 9.02,P=0.05).Conclusion NDP combined with GEM in advanced lung squamous cell carcinoma of the first-line treatment has equivalent efficacy to DDP+GEM, with lower incidence of adverse reaction,which is worthy of further dissemination of research.

Objective : To investigate the effect of teriparatide ( TPTD) on the generation of MC3T3-E1 cells to- wards osteogenic differentiation via the Wnt3a / β-catenin pathway in a high-glucose environment． Methods : The experiment was divided into five groups : low glucose group，low glucose + TPTD group，high glucose group，high glucose + TPTD group，high glucose + TPTD + Wnt3a inhibitor G244-LM group．Cell proliferation activity was de- tected by Calcein-AM and CCK-8 assay，cell mineralized nodule formation was observed by ALP and alizarin red staining，and actin formation was analyzed by immunofluorescence assay. Real-time PCR was performed to detect Wnt3a，β-catenin，Tcf1，OPG and COL Ⅰ mRNA expression. Results : TPTD had no significant effect on the pro- liferative activity of MC3T3-E1 cells under high glucose condition．The ALP staining area，protein activity and aliza- rin red staining area of the cells in the low glucose + TPTD group were higher than those in the other four groups (P ＜0. 05) ; the high glucose group was lower than the low glucose group (P ＜0. 05 ) ; the high glucose + TPTD group was higher than the high glucose group and the high glucose + TPTD + G244-LM group (P＜0. 05) ．The cy- toskeleton in the low glucose + TPTD group was the clearest ; the cytoskeleton was less clear in both the high glucose and high glucose + TPTD + G244-LM groups than in the high glucose + TPTD group．Genes such as Wnt3a，β-cate- nin，Tcf1，OPG and COL Ⅰ had the highest mRNA levels in the cells of the low glucose + TPTD group (P < 0. 05) ; the mRNA levels of all genes were higher in the low glucose group than thosein the high glucose group (P ＜0. 05) ; the mRNA levels of all genes in the cells of the high glucose + TPTD group were higher than those in the high glucose group and the high glucose + TPTD + G244-LM group ( P＜0. 05) ． Conclusion High glucose inhibi- ted osteoblast differentiation，and TPTD promoted osteoblast differentiation in high glucose environment by regula- ting Wnt3a / β-catenin pathway.